This document discusses non-migratory bioactive polymers for food packaging. It provides examples of how bioactive peptides and antimicrobial peptides can be covalently linked to packaging polymers to inhibit microbial growth without migrating into the food. Specifically, it mentions how chitosan, UV-irradiated nylon, and nylon treated with laser can all exhibit antimicrobial properties through interactions with microbial membranes that disrupt permeability. Non-migratory bioactive polymers provide benefits like improved stability of bioactive compounds, regulatory advantages over food additives, and enabling minimally processed foods with a longer shelf life.

1. NON MIGRATORY
BIOACTIVE POLYMERS
SUBMITTED TO: SUBMITTED BY:
Ms. NEHA SINGH KUSH VERMA
15/PFT/001

2. INTRODUCTION
• Non-migratory bioactive packaging is a novel
form of active packaging.
• It gives desirable biological response to food
without the active component migrating from
the packaging into the food.

3. • Bioactive peptides were covalently linked to the
free terminus of the PEG chains.
• Antibacterial activity was found for films treated
with antimicrobial peptide.
• No activity was found for lactase attached to PE
films.
• NMBP possess biological activity without the
active components migrating from the polymer
to the substrate.
• Example: inhibition of microbial growth.

4. Types of Non-Migratory
Bioactive Polymers
Chitosan
a) Possesses broad spectrum antimicrobial
activity.
b) Commercially available as an antifungal
coating for fresh fruit.
c) Chitosan is the deacetylated form of chitin.
d) Effective against gram-positive and gram-
negative bacteria.

5. Interaction b/w polycationic chitosan and anions
Change in membrane permeability
Bacterial outer membrane gets disrupted
Leakage of glucose lactate dehydrogenase
(+ve charge exist on amino group of C₂ below pH 6.3)

6. UV irradiated nylon
a) The antimicrobial effect is strongly dependent on
the UV wavelength used, the temperature and
the composition.
b) Example- poly-L-lysine
c) Interaction with negatively charged microbial
membranes leading to membrane disruption.
PEG It posses antimicrobial activity only at
high concentrations. It reduces the adhesion
of proteins and cells to polymers.

7. Nylon
Lazer treatment at 193nm
Amides converted into amines and binds with polymer chain
Interaction with –vely charged microbial membrane leads to
membrane disruption
Leakage of cellular constituents

8. APPLICATION
• In-package processing.
• Antimicrobial packaging .
• Shelf life extension.
• Intelligent Packaging.

9. ADVANTAGES
1. Technical benefits
a) Improved stability and conc. of bioactive
compounds and its effect at a specific area.
b) Immobilization of bioactive compounds.
c) Conc. Of bioactive molecules are higher on
the surface of polymer and headspace.

10. 2. Regulatory advantages
a) A/C to US regulation that there is negligible
migration into a food product is not classified
as a food additive.
b) NMBP don’t need to meet food additive
regulation.

11. 3. Marketting aspects
a) It incorporates non migratory antimicrobials
in packaging material.
b) It provide minimally processed, preservative
free food products.
c) More demand.

12. 4. Food processor’s perspective
a) More stable pdt. with longer shelf life.
b) Using lactase-active packaging, milk could be
packed off a normal production run and a
lactose reduced product obtained after a
short period of storage. Eg- lactose free milk.
c) It has high cost and low sales volume.

13. THANK YOU