Skip to main content
UploadLanguage (EN)Support
BusinessMobileSocial MediaMarketingTechnologyArt & PhotosCareerDesignEducationPresentations & Public SpeakingGovernment & NonprofitHealthcareInternetLawLeadership & ManagementAutomotiveEngineeringSoftwareRecruiting & HRRetailSalesServicesScienceSmall Business & EntrepreneurshipFoodEnvironmentEconomy & FinanceData & AnalyticsInvestor RelationsSportsSpiritualNews & PoliticsTravelSelf ImprovementReal EstateEntertainment & HumorHealth & MedicineDevices & HardwareLifestyle
RB
Uploaded byRakesh Barik
PPTX, PDF6,123 views

Taxol, Vincristine, Vinblastine.pptx pharmacognosy

1) The document discusses the production, estimation, and utilization of taxol, vincristine, and vinblastine, which are phytochemicals obtained from plant sources. 2) Taxol is extracted from the bark of the Pacific Yew tree through methanol extraction and partitioning. HPLC and HPTLC methods are used for its estimation. Taxol is used to treat various cancers. 3) Vincristine and vinblastine are indole alkaloids obtained from the plant Catharanthus roseus through a two-stage plant tissue culture fermentation process. Ethyl acetate extraction is used to isolate the compounds, which are then estimated using HPLC and TLC. They are used

Education◦
Download to read offline
INDUSTRIAL PRODUCTION, ESTIMATION AND UTILIZATION OF PHYTOCONSTITUENTS Taxol, Vincristine & Vinblastine
TAXOL Biological Source: Taxol is a diterpenoid obtained from the bark of Pacific Yew Tree, Taxus brevifolia, belonging to family Taxaceae. 2
TAXOL ⚫Production: Method -1 • Powdered bark extracted with methanol, filtered & evaporated 1 to dryness. 2 • Partitioned with the mixture of carbon tetrachloride & water, filter & evaporated. 3 • Dried CCl4 fraction again extracted with CCl4 : methanol, evaporate to obtain crude taxol. 3
1) The dried ground bark is extracted with methanol or ethanol , and alcohol is removed by concentrating the combined extract. 2) The obtained concentrate is re -extracted with dichloromethane and the solvent extract is concentrated to a powder. 3) This powder is stirred with a mixture of acetone and ligroin (1:1) and filtered to remove the insoluble matter. 4) The filtrate containing taxol is concentrated, dissolved in 30% acetone in ligroin, and applied to a column of Florisil. 5) The taxol fraction from the column is twice purified by crystallisation. 6) The crystalline taxol is subjected to chromatography on a silica column. 7) The purified taxol obtained from the column is crystallised twice. 8) The unseparated mixtures and mother liquors are recycled through the silica column to obtain more pure taxol. Method - 2
HPLC Method Method: Isocratic Detector: Photodiode array detector Stationery Phase: C18 Column Mobile Phase: 0.02 M Potassium dihydrogen phosphate (buffer solution) in water (pH 4.5 adjusted with potassium hydroxide) and acetonitrile in the ratio of 60:40 v/v. Flow rate: 2ml/min. Run time: 8.0 min Detection: 230 nm. Estimation:
TAXOL ⚫ Estimation: HPTLC method Mob phase- chloroform:methanol (7:3v/v) Visualizing agent- vanillin sulphuric acid. ⚫ Utilization: 1. Treatment of ovarian, lung, bladder, esophageal & other types of cancers. 2. Antiproliferative agent. 6
VINCRISTINE & VINBLASTINE ⚫ Biological Source: These are Indole alkaloids obtained from the dried whole plant of Catharanthus roseus or Vinca rosea, belonging to family- Apocynaceae. 7
⚫ Production: Plant tissue culture technique. A two stage fermentation procedure was used. 500 ml Erlenmeyer flasks containing 100 ml medium (0.3%) malt extract, (1.0%) glucose, (0.3%) yeast extract and (0.5%) peptone) were inoculated with 7 days old culture and incubated at 28°C on a rotary shaker (240 rpm) for 4–5 days, which was used as seed culture. 10 ml seed culture was transferred to 500 ml Erlenmeyer flask containing 100 ml production medium called as vinca medium-1 (Glucose: 3%, Succinic acid: 1%, Sodium benzoate: 100 mg, Peptone: 1%, Magnesium sulphate: 3.6 mg, Biotin: 1 mg, Thiamine: 1 mg, Pyridoxal: 1 mg, Calcium pentothenate: 1 mg, Phosphate buffer: 1 ml (pH 6.8), L-Tryptophan: 0.1%, Geranium oil: 0.05%.) which were incubated at 28°C for 20 days as shake culture (II stage), after which it was harvested and used for further study. 9
Culture filtrates and mycelia were separated with the help of muslin cloth and then lyophilized. Lyophilized culture filtrate was extracted using ethyl acetate as a solvent system. The organic layer was separated from the aqueous layer using separating funnel. The extraction was repeated thrice and the solvent was dried using anhydrous sodium sulphate and concentrated under vacuum using rotavapour at 40°C in order to get crude extract. Estimation: ⚫ HPLC : Mob phase- acetonitrile: 0.1 M phosphate buffer. Wavelength- 254nm.
Estimation: ⚫ TLC: Sample: A small amount of crude extract was dissolved in ethyl acetate. Stationery Phase: silica gel-G (0.5 mm thickness) Mobile Phase: Chloroform∶methanol (8∶2) as a solvent system. Detection: Sprayed with ceric ammonium sulphate reagent. Vinca alkaloids spots produced brilliant violet color as well as purple color. Utilization: 1. In chemotherapy regimens 2. Childhood leukemia 3. Immunosuppressant 4. In Hodgkin’s disease