Bioethics in rDNa technology

1. Submitted to:
Ms.P.Mala
Assistant Professor
Department of Biotechnology
Periyar Maniammai University of
Science & Technology
Recombinant DNA Technology
Topic: Bioethics in rDNA
technology
Present by:
K.Praveena
123011356016
Ms.c.Biotechnology
I-Year
YBT-201

2. INTRODUCTION
• Bioethics is the study of ethical issues arising from advances in biology
and medicine.
• It involves analyzing the moral implications of topics like genetic
engineering , euthanasia , organ donation, and reproductive technologies.
• Bioethicists seek to balance scientific progress with ethical principles,
considering factors like autonomy, beneficence, justice, and non-
maleficence. It's a field that intersects with philosophy, medicine, law, and
other disciplines to guide ethical decision-making in healthcare and
research.

3. PRINCIPLE OF BIOETHICS
• The four main principles of bioethics are:
• Autonomy: Respecting the decision-making capacity of
individuals and allowing them to make choices about their own
healthcare and lives.
• Beneficence: Acting in ways that benefit others and promote
their well-being.
• Nonmaleficence: Avoiding harm to others and minimizing risks
of harm.
• Justice: Ensuring fairness and equality in the distribution of
resources and access to healthcare services.

4. BIOETHICS IN RDNA TECHNOLOGY
The gene of interest is cut from the genome using restriction
enzymes.
It is then joined with a similarly cut DNA molecule,a plasmid .
The plasmid is known as the cloning vectors .
Plasmids are circular molecules found in bacteria that are
separate from the bacterium normal genome .
Plasmids are self-replicating,allowing the new recombinant
DNA molecule to produce its gene product in its news
environment.

5. Plasmids are not only cloning vectors that can be used ,but
they are very common.
Each vector has limitation to the size (in base pairs) of the DNA
fragment that can be cloned .
This technology has been used since the 1970s;it is fairly
common practice in molecular biology labs today.

6. BIOETHICS IN BIOTECHNOLOGY
• Bioethics in biotechnology involves addressing ethical
considerations and dilemmas arising from advancements in
biotechnological research and applications. Some key areas of
focus within bioethics in biotechnology include:
• Genetic Engineering: Ethical concerns surround genetic
manipulation techniques like CRISPR-Cas9, gene editing, and
genetic modification of organisms.
• Biobanks and Big Data: The collection, storage, and use of
biological samples and health data in biobanks raise ethical
concerns related to privacy, consent, data ownership, and the
potential for discrimination or exploitation.

7. • Cloning: The cloning of animals and the potential for human
cloning raise ethical questions related to individual identity,
human dignity, and the instrumentalization of life. Issues of
consent, autonomy, and the welfare of cloned individuals are
also central to ethical discussions in this area.
• Stem Cell Research: Ethical debates in stem cell research
involve the use of embryonic stem cells, adult stem cells, and
induced pluripotent stem cells (iPSCs).
• Synthetic Biology: The engineering of biological systems at
the molecular level raises ethical questions about the creation
of synthetic organisms, biosecurity risks, environmental impact,
and the implications for biodiversity and ecosystems.

8. APPLICATIONS
• Clinical Decision Making
• Research Ethics
• Reproductive Rights
• Genetic Testing and Counseling
• End-of-Life Care
• Public Health
• Environmental Ethics
• Biomedical Research and Innovation

9. ADVANTAGES OF BIOETHICS
• Guidance in Decision Making
• Protection of Rights and Dignity
• Promotion of Beneficence and Nonmaleficence
• Ethical Research Practices
• Addressing Emerging Ethical Challenge
• Ethical Innovation and Progress
• Interdisciplinary Collaboration
• Promotion of Public Discourse and Education
• Promotion of Justice and Equity

10. DISADVANTAGE OF BIOETHICS
• Subjectivity and Interpretation
• Cultural and Moral Diversity
• Complexity and Uncertainty
• Potential for Stifling Innovation
• Conflict with Religious Beliefs
• Resource Allocation Challenges
• Inconsistencies in Application
• Regulatory Challenges

11. CONCLUSION
• In conclusion, while bioethics plays a crucial role in guiding
ethical decision-making in healthcare and biotechnology, it is
not without its challenges and limitations.
• Despite efforts to establish universal ethical standards, cultural
biases, complex debates, conflicts with practical considerations,
moral distress, regulatory challenges, and limited accessibility
remain significant disadvantages of bioethics.
• Nevertheless, recognizing these limitations allows for ongoing
dialogue and refinement of ethical frameworks to address
emerging ethical dilemmas in an ever-evolving technological
and societal landscape.

12. MCQ
1.What is bioethics?
A) Study of biology
B) Study of ethical issues in medicine and biology
C) Study of environmental conservation
D) Study of genetics
2.What can be a potential disadvantage of bioethics in multicultural
societies?
A) Universal acceptance of ethical principles
B) Conflicts between ethical principles and cultural norms
C) Limited accessibility of ethical guidelines
D) Lack of relevance in diverse cultures

13. 3.What term describes the distress experienced by healthcare
professionals when ethical principles clash with real-world
constraints?
A) Ethical conflict
B) Cultural bias
C) Moral distress
D) Professional dilemma
4.Which practical consideration may sometimes conflict with
ethical ideals in healthcare decision-making?
A) Resource allocation
B) Universality of ethical principles
C) Moral absolutism
D) Ethical relativism

14. 5.What is the role of bioethics in healthcare and biotechnology?
A) Setting rigid ethical rules
B) Providing a framework for analyzing ethical dilemmas
C) Ignoring cultural differences
D) Dictating medical practices
ANSWER:
1.B
2.B
3.C
4.A
5.B

15. REFRENCE
• Definition of ETHOS”. Merriam-webster.com. Retrieved 25
December 2022.
• Bracanovic, T (June 2012). “From integrative bioethics to
pseudoscience”. Developing World Bioethics. 12 (3): 148–156.
doi:10.1111/j.1471-8847.2012.00330.x. PMID 22708689.
• Kaçar B (20 November 2020). “If we’re alone in the Universe,
should we do anything about it?”. Aeon. Retrieved 11 December
2020.

16. THANK YOU