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GENETIC CODE
Dr. Mahesha H B
Associate Professor and Head
Department of Sericulture
Yuvaraja’s College
University of Mysore, Mysuru, India
3 September 2017 1
www.hbmahesh.weebly.com
GENETIC CODE
Central Dogma of Molecular Biology
3 September 2017 2
www.hbmahesh.weebly.com
DNA Amino Acids
General Structure of DNA and Amino Acids showing no specific recognition sites
Hence needs an adaptor molecule i.e., tRNA
3 September 2017 3
www.hbmahesh.weebly.com
tRNA structure and role in translation
3 September 2017 4
www.hbmahesh.weebly.com
tRNA acts as an adaptor
molecule in translation
between DNA amino acids
3 September 2017 5
www.hbmahesh.weebly.com
Genetic code:The sequence of nucleotides that determines the
specific amino acid sequence in the synthesis of proteins.
3 September 2017 6
www.hbmahesh.weebly.com
Codon: A section of DNA (three nucleotide pairs in
length) or RNA (three nucleotide in length) that
codes for a single amino acid.
Or
A sequence of three RNA or DNA nucleotides that
specifies (codes for) either an amino acid or the
termination of translation.
termination of translation.
Or
A base sequence corresponding to a particular
amino acid is called codon.
3 September 2017 7
www.hbmahesh.weebly.com
Genetic Code
3 September 2017 8
www.hbmahesh.weebly.com
Major Properties
1. Triplet:
Gamow 1954 suggested 3 letter code
3 September 2017 9
www.hbmahesh.weebly.com
Genetic code is Non-Overlapping,
if it is overlapping three amino acids
Should have been changed in sickle cell
anemia
Non Overlapping
Non Overlapping
3 September 2017 10
www.hbmahesh.weebly.com
3. Comma less:
Genetic code is comma less
3 September 2017 11
www.hbmahesh.weebly.com
4.Has Polarity:
Genetic code has polarity i.e., 5’ to 3’ direction.
If it reads in opposite direction it yields a different protein
3 September 2017 12
www.hbmahesh.weebly.com
5. Universal: Marshal et al., 1967
In Yeast mitochondria
UGA codes for
tryptophan, although in
the nuclear genes UGA is
a termination codon.
a termination codon.
In the ciliate protozoa
UAA & UAG codes for
glutamine instead of
stop signal
3 September 2017 13
www.hbmahesh.weebly.com
6. Degenerate/Redundant:
Provision or existence of more than one Codon to
perform an activity or function.
3 September 2017 14
www.hbmahesh.weebly.com
7. Non-ambiguous/unambiguous:
Having or exhibiting no ambiguity or
uncertainty; clear
3 September 2017 15
www.hbmahesh.weebly.com
Wobble Hypothesis
Crick – 1966 proposed hypothesis
3 September 2017 16
www.hbmahesh.weebly.com
3 September 2017 17
www.hbmahesh.weebly.com
3 September 2017 18
www.hbmahesh.weebly.com
Acknowledgements
to
INTERNET
FOR
PICTURES
3 September 2017 19
www.hbmahesh.weebly.com

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genetic_code_ppt..pdf

  • 1. GENETIC CODE Dr. Mahesha H B Associate Professor and Head Department of Sericulture Yuvaraja’s College University of Mysore, Mysuru, India 3 September 2017 1 www.hbmahesh.weebly.com
  • 2. GENETIC CODE Central Dogma of Molecular Biology 3 September 2017 2 www.hbmahesh.weebly.com
  • 3. DNA Amino Acids General Structure of DNA and Amino Acids showing no specific recognition sites Hence needs an adaptor molecule i.e., tRNA 3 September 2017 3 www.hbmahesh.weebly.com
  • 4. tRNA structure and role in translation 3 September 2017 4 www.hbmahesh.weebly.com
  • 5. tRNA acts as an adaptor molecule in translation between DNA amino acids 3 September 2017 5 www.hbmahesh.weebly.com
  • 6. Genetic code:The sequence of nucleotides that determines the specific amino acid sequence in the synthesis of proteins. 3 September 2017 6 www.hbmahesh.weebly.com
  • 7. Codon: A section of DNA (three nucleotide pairs in length) or RNA (three nucleotide in length) that codes for a single amino acid. Or A sequence of three RNA or DNA nucleotides that specifies (codes for) either an amino acid or the termination of translation. termination of translation. Or A base sequence corresponding to a particular amino acid is called codon. 3 September 2017 7 www.hbmahesh.weebly.com
  • 8. Genetic Code 3 September 2017 8 www.hbmahesh.weebly.com
  • 9. Major Properties 1. Triplet: Gamow 1954 suggested 3 letter code 3 September 2017 9 www.hbmahesh.weebly.com
  • 10. Genetic code is Non-Overlapping, if it is overlapping three amino acids Should have been changed in sickle cell anemia Non Overlapping Non Overlapping 3 September 2017 10 www.hbmahesh.weebly.com
  • 11. 3. Comma less: Genetic code is comma less 3 September 2017 11 www.hbmahesh.weebly.com
  • 12. 4.Has Polarity: Genetic code has polarity i.e., 5’ to 3’ direction. If it reads in opposite direction it yields a different protein 3 September 2017 12 www.hbmahesh.weebly.com
  • 13. 5. Universal: Marshal et al., 1967 In Yeast mitochondria UGA codes for tryptophan, although in the nuclear genes UGA is a termination codon. a termination codon. In the ciliate protozoa UAA & UAG codes for glutamine instead of stop signal 3 September 2017 13 www.hbmahesh.weebly.com
  • 14. 6. Degenerate/Redundant: Provision or existence of more than one Codon to perform an activity or function. 3 September 2017 14 www.hbmahesh.weebly.com
  • 15. 7. Non-ambiguous/unambiguous: Having or exhibiting no ambiguity or uncertainty; clear 3 September 2017 15 www.hbmahesh.weebly.com
  • 16. Wobble Hypothesis Crick – 1966 proposed hypothesis 3 September 2017 16 www.hbmahesh.weebly.com
  • 17. 3 September 2017 17 www.hbmahesh.weebly.com
  • 18. 3 September 2017 18 www.hbmahesh.weebly.com