The study evaluates twelve spring wheat genotypes for water use efficiency (WUE) under varying water regimes in Zambia, identifying genotypes such as Sahai I and Loerie II with the highest WUE and grain yield. The research demonstrates significant genetic variation in traits like harvest index and thousand kernel weight, which influence WUE, making them valuable for breeding programs aimed at improving drought resistance. The findings suggest that adopting water-efficient wheat varieties could enhance production amidst increasing irrigation costs and water scarcity.