Transforming Maize-legume Value Chains –A Business Case for Climate-Smart Ag...CIMMYT
CIMMYT Senior Cropping Systems Agronomist Christian Thierfelder presented on climate-smart agriculture in southern Africa in a webinar titled Climate Resilient Agriculture Success Stories – Making a Case for Scale Up.
Adoption of good agricultural practices for sustainable maize and cowpea prod...Premier Publishers
This paper presents a comparative evaluation of maize and cowpea grain yields of 24 farmers in the Forest-Savannah Transition (Atebubu-Amantin) and Guinea Savannah (West-Mamprusi) zones of Ghana for 2012 and 2014 respectively at the inception and completion of the sustainable intensification of crop-livestock integration project. While only 7 and 2 farmers planted maize in rows at project inception, 12 and 9 farmers were recorded at project completion in Atebubu–Amantin and West–Mamprusi districts respectively. During 2012 cropping season, when farmers had access to fertilizer in the form of subsidy, only a farmer in each district did not apply fertilizer. However, the reverse was observed in 2014 where there was no subsidy. Comparing 2012 and 2014 cropping season results, revealed a 25% and 27% increase in maize grain yield in Atebubu–Amantin and West–Mamprusi districts respectively. Economic analysis revealed a high benefit cost ratio in maize and cowpea production in 2014 than in 2012 for both locations. The study has demonstrated that, enabling environment that encourages access to tools and implements for row planting and fertilizer in the form of subsidy would complement good agronomic technology packages introduced to farmers in order to ensure sustainable cereal and legume production.
Yogendra Katuwal on Influence of N levels on Yield of Improved and Hybrid Ric...Yogendra Katuwal
Not always increasing N dose can be economical. Use efficiency of hybrids and improved varieties vary greatly.
Prepared By:
Yogendra Katuwal a MSc.Ag (Agronomy) student in AFU, Rampur, Nepal.
Transforming Maize-legume Value Chains –A Business Case for Climate-Smart Ag...CIMMYT
CIMMYT Senior Cropping Systems Agronomist Christian Thierfelder presented on climate-smart agriculture in southern Africa in a webinar titled Climate Resilient Agriculture Success Stories – Making a Case for Scale Up.
Adoption of good agricultural practices for sustainable maize and cowpea prod...Premier Publishers
This paper presents a comparative evaluation of maize and cowpea grain yields of 24 farmers in the Forest-Savannah Transition (Atebubu-Amantin) and Guinea Savannah (West-Mamprusi) zones of Ghana for 2012 and 2014 respectively at the inception and completion of the sustainable intensification of crop-livestock integration project. While only 7 and 2 farmers planted maize in rows at project inception, 12 and 9 farmers were recorded at project completion in Atebubu–Amantin and West–Mamprusi districts respectively. During 2012 cropping season, when farmers had access to fertilizer in the form of subsidy, only a farmer in each district did not apply fertilizer. However, the reverse was observed in 2014 where there was no subsidy. Comparing 2012 and 2014 cropping season results, revealed a 25% and 27% increase in maize grain yield in Atebubu–Amantin and West–Mamprusi districts respectively. Economic analysis revealed a high benefit cost ratio in maize and cowpea production in 2014 than in 2012 for both locations. The study has demonstrated that, enabling environment that encourages access to tools and implements for row planting and fertilizer in the form of subsidy would complement good agronomic technology packages introduced to farmers in order to ensure sustainable cereal and legume production.
Yogendra Katuwal on Influence of N levels on Yield of Improved and Hybrid Ric...Yogendra Katuwal
Not always increasing N dose can be economical. Use efficiency of hybrids and improved varieties vary greatly.
Prepared By:
Yogendra Katuwal a MSc.Ag (Agronomy) student in AFU, Rampur, Nepal.
Title: ‘Differentiated Agronomies’ for Sustainable Rice Intensification : Towards an Alternative Policy Framework for Local Food Security in India
Authors: Ravindra Adusumilli, Debashish Sen, Sabarmatee, C. Shambu Prasad, Rob Schipper, Raj Kumar Kumawat
Presented at: First International Conference on Global Food Security
Venue: Noordwijkerhout, The Netherlands, 29th September to 2nd Oct, 2013
Presented at the Pulses for Sustainable Agriculture and Human Health” on 31 May-1 June 2016 at NASC, New Delhi, India. The conference was jointly organised by the International Food Policy Research Institute (IFPRI), National Academy of Agricultural Sciences (NAAS), TCi of Cornell University (TCi-CU) and Agriculture Today.
Options for intensifying cereal-legume cropping systems in West Africa africa-rising
Poster prepared by Asamoah Larbi, Abdul Rahman Nurudeen, Saaka Buah, Roger Kanton2 and Bekele Kotu for the Africa RISING Science for Impact Workshop, Dar es Salaam, 17-19 January 2017
Achievements on pulses & oilseed research in BRAC have been described. #suggested link of my you tube video on mung bean: https://www.youtube.com/watch?v=7iEszjkUab4&t=8s and the link of lentil cultivation: https://www.youtube.com/watch?v=s3DfknzVx_c #also the link of sesame cultivation: https://www.youtube.com/watch?v=BAFjWsPetK8&t=79s
Presenter: Norman Uphoff
Title: Agroecological Strategies for Raising Crop Productivity with Reduced Inputs, with Less Water Requirement, and with Buffering of Climate-Change Stresses
Date: April 10, 2014
Venue: Agricultural Research Center, Sakha, Kafr El-Sheikh, Egypt
Bangladesh though a LDC have generated national database on Land and Soil Resources which is mainly used for agricultural development planning and farmers service. SOLARIS and OFRS are two systems dealing with the NR database to generate output needed by academicians, researchers, extentionists and farmers
Performance of wheat based intercropping as influenced by row proportions und...inventionjournals
A field experiment was conducted on various wheat based intercropping system in different row proportions under rainfed condition of Kaymore plateau at Krishi Vigyan Kendra, Deendayal Research Institute, Majhagawan, Satna in rabi season 2013 and 2014.The intercropping of wheat was done with chickpea, linseed and mustard with row proportions of 2:2, 4:2 and 6:2.The experiment was laid out in 3 replications containing 13 treatment combinations. The observations were recorded at 30,60,90 DAS and at maturity in sole as well as on intercrops. On the basis of results obtained the intercropping of wheat with chickpea proved to be superior in terms of wheat equivalent yield and economics.
Sustainable intensification of groundnut production in northern Ghanaafrica-rising
Poster prepared by Abdul Rahman Nurudeen, Larbi Asamoah, Kizito Fred, Kotu Bekele and Hoeschle-Zeledon Irmgard at the Africa RISING Program Learning Event, 5-8 February 2019.
Farmers’ uptake of improved feed practices and reasons for adoption/ non adop...ILRI
Presented by Gregory Ndwandwa Sikumba at the CLEANED Project East Africa Stakeholder Consultation on Dairy and Environment Nairobi, Kenya, 18 September 2013
Title: System of Rice Intensification
Presented by: Erika Styger
Presented at: Special Exhibit/Event on Rice Production at Agritechnica
Venue and Date: Hannover, Germany November 15, 2013
Presenter: Ram Bahadur Khadka
Title: New Directions for the System of Rice Intensification in Nepal: Mechanization and Biofertilizers
Date: December 9, 2016
Venue: Mann 102, Cornell University, Ithaca, NY
Asia Regional Program Planning Meeting- Achieving self sufficiency in pulse p...ICRISAT
On average, over the last three years Indian’s consumed approximately 22 million tonnes of pulses per annum but produced only 18 million tonnes, leaving a shortfall of 4 million tonnes.Pulse self-sufficiency means food security, greater wealth for Indian farmers and a more favourable balance of trade for the nation.Protein energy malnutrition (PEM) is a major public health problem in India. The prevalence of stunting among under fives is 48% and wasting is 19.8% and with an underweight prevalence of 42.5%, it is the highest in the world.
Intensifying legume/cereal cropping systems in Malawiafrica-rising
Presentation by Regis Chikowo, Christian Thierfelder, Wezi Mhango and Rowland Chirwa at the Africa RISING ESA Project Review and Planning Meeting, Dar es Salaam, Tanzania, 11-12 September 2019.
Crop Nutrient Gap Project
Update presented on September 15, 2017 to CCAFS at Wageningen University and Research Centre.
In association with IFA, University of Nebraska, YARA, and Global Yield Gap Atlas.
Title: ‘Differentiated Agronomies’ for Sustainable Rice Intensification : Towards an Alternative Policy Framework for Local Food Security in India
Authors: Ravindra Adusumilli, Debashish Sen, Sabarmatee, C. Shambu Prasad, Rob Schipper, Raj Kumar Kumawat
Presented at: First International Conference on Global Food Security
Venue: Noordwijkerhout, The Netherlands, 29th September to 2nd Oct, 2013
Presented at the Pulses for Sustainable Agriculture and Human Health” on 31 May-1 June 2016 at NASC, New Delhi, India. The conference was jointly organised by the International Food Policy Research Institute (IFPRI), National Academy of Agricultural Sciences (NAAS), TCi of Cornell University (TCi-CU) and Agriculture Today.
Options for intensifying cereal-legume cropping systems in West Africa africa-rising
Poster prepared by Asamoah Larbi, Abdul Rahman Nurudeen, Saaka Buah, Roger Kanton2 and Bekele Kotu for the Africa RISING Science for Impact Workshop, Dar es Salaam, 17-19 January 2017
Achievements on pulses & oilseed research in BRAC have been described. #suggested link of my you tube video on mung bean: https://www.youtube.com/watch?v=7iEszjkUab4&t=8s and the link of lentil cultivation: https://www.youtube.com/watch?v=s3DfknzVx_c #also the link of sesame cultivation: https://www.youtube.com/watch?v=BAFjWsPetK8&t=79s
Presenter: Norman Uphoff
Title: Agroecological Strategies for Raising Crop Productivity with Reduced Inputs, with Less Water Requirement, and with Buffering of Climate-Change Stresses
Date: April 10, 2014
Venue: Agricultural Research Center, Sakha, Kafr El-Sheikh, Egypt
Bangladesh though a LDC have generated national database on Land and Soil Resources which is mainly used for agricultural development planning and farmers service. SOLARIS and OFRS are two systems dealing with the NR database to generate output needed by academicians, researchers, extentionists and farmers
Performance of wheat based intercropping as influenced by row proportions und...inventionjournals
A field experiment was conducted on various wheat based intercropping system in different row proportions under rainfed condition of Kaymore plateau at Krishi Vigyan Kendra, Deendayal Research Institute, Majhagawan, Satna in rabi season 2013 and 2014.The intercropping of wheat was done with chickpea, linseed and mustard with row proportions of 2:2, 4:2 and 6:2.The experiment was laid out in 3 replications containing 13 treatment combinations. The observations were recorded at 30,60,90 DAS and at maturity in sole as well as on intercrops. On the basis of results obtained the intercropping of wheat with chickpea proved to be superior in terms of wheat equivalent yield and economics.
Sustainable intensification of groundnut production in northern Ghanaafrica-rising
Poster prepared by Abdul Rahman Nurudeen, Larbi Asamoah, Kizito Fred, Kotu Bekele and Hoeschle-Zeledon Irmgard at the Africa RISING Program Learning Event, 5-8 February 2019.
Farmers’ uptake of improved feed practices and reasons for adoption/ non adop...ILRI
Presented by Gregory Ndwandwa Sikumba at the CLEANED Project East Africa Stakeholder Consultation on Dairy and Environment Nairobi, Kenya, 18 September 2013
Title: System of Rice Intensification
Presented by: Erika Styger
Presented at: Special Exhibit/Event on Rice Production at Agritechnica
Venue and Date: Hannover, Germany November 15, 2013
Presenter: Ram Bahadur Khadka
Title: New Directions for the System of Rice Intensification in Nepal: Mechanization and Biofertilizers
Date: December 9, 2016
Venue: Mann 102, Cornell University, Ithaca, NY
Asia Regional Program Planning Meeting- Achieving self sufficiency in pulse p...ICRISAT
On average, over the last three years Indian’s consumed approximately 22 million tonnes of pulses per annum but produced only 18 million tonnes, leaving a shortfall of 4 million tonnes.Pulse self-sufficiency means food security, greater wealth for Indian farmers and a more favourable balance of trade for the nation.Protein energy malnutrition (PEM) is a major public health problem in India. The prevalence of stunting among under fives is 48% and wasting is 19.8% and with an underweight prevalence of 42.5%, it is the highest in the world.
Intensifying legume/cereal cropping systems in Malawiafrica-rising
Presentation by Regis Chikowo, Christian Thierfelder, Wezi Mhango and Rowland Chirwa at the Africa RISING ESA Project Review and Planning Meeting, Dar es Salaam, Tanzania, 11-12 September 2019.
Crop Nutrient Gap Project
Update presented on September 15, 2017 to CCAFS at Wageningen University and Research Centre.
In association with IFA, University of Nebraska, YARA, and Global Yield Gap Atlas.
Genetic Enhancement of Lentil for Adaptation to Various Cropping Systems an...ICARDA
Genetic Enhancement of Lentil for Adaptation to Various Cropping Systems and Nutritional Security in South Asia
2-13 September 2019. New Delhi, India. Over 8,000 participants from all over the world participated in COP14.
Presentation by Ashutosh Sarker
Presented at the Pulses for Sustainable Agriculture and Human Health” on 31 May-1 June 2016 at NASC, New Delhi, India. The conference was jointly organised by the International Food Policy Research Institute (IFPRI), National Academy of Agricultural Sciences (NAAS), TCi of Cornell University (TCi-CU) and Agriculture Today.
Intensification of maize-legume based systems in the semi-arid areas of Tanza...africa-rising
Presented by Ganga Rao, NVRP, Kimaro, A., Makumbi, D., Mponda, O., Msangi, R., Rubanza, C.D., Seetha, A., Swai, E. and Okori, P. at the Africa RISING East and Southern Africa annual review and planning meeting, Lilongwe, Malawi, 3-5 September 2013
Integrated soil fertility management affect profitability of soybean in north...africa-rising
Poster prepared by Larbi, A., AddulRahman, N., Kotu, B., Hoeschle-Zeledon, I., Akakpo, D.B. and Mellon S.B. for the AfrIca RISING West Africa Review and Planning Meeting, Accra, 30 March–1 April 2016
Presented By: Tareke Berhe, Ayele G. Ayetenfisu,
Zewdie Gebretsadik, and Norman Uphoff
Title: The System of Tef Intensification:
Opportunities for greater food security in Ethiopia, and elsewhere, through modifications in crop management
Date: October 13, 2015
Venue: 2nd International Conference on Global Food Security, Cornell University
Effects of soil management and cropping options on yields of drought tolerant...africa-rising
Poster prepared by Gift Ndengu, Powell Mponela., Barthlomew Chataika, Rowland Chirwa and Lulseged Tamene for the Africa RISING Science for Impact Workshop, Dar es Salaam, 17-19 January 2017
Presented by Hongmin Dong and Sha Wei, Chinese Academy of Agricultural Sciences (CAAS), on 28 June 2021 at the Asian Development Bank (ADB) Webinar on Sustainable Protein Case Study: Outputs and Synthesis of Results.
Presented by Ben Lukuyu, Leonard Marwa, Chrispinus Rubanza, Anthony Kimaro and Christopher Mutungi at at the Africa RISING ESA Project Review and Planning Meeting, Dar es Salaam, Tanzania, 11-12 September 2019.
Performance of Hybrid and Conventional Rice Varieties in Sindhsanaullah noonari
The study was design to compare the economic performance of hybrid and conventional rice production, major
objectives of the study were to asses financial gain from hybrid rice comparing with conventional rice and
Taluka Golarchi was selected for the present study where both on hybrid and conventional rice varieties are
grown, primary data on hybrid and conventional rice was collected from the farmers through personal interviews
with the help of specially designed questionnaire. A simple random sampling technique was used to collect the
data. Statistical approaches used to analysis the data. Total costs per hectare of hybrid rice were 148992.23 Rs
per hectare which were more then conventional rice was 140661.68 Rs per hectactare. Major differences in
hybrid rice production cost are related to higher seed prices, slightly higher land management costs. On an
average higher yield (196.14 monds per hectare) was obtained from hybrid rice while conventional rice yield
(140.14 monds per hectare) was less then hybrid rice. There was 16.64 percent increase in hybrid rice yield
comparing with conventional rice which gives additional income to poor farmers, Price gained per mounds was
almost the same in both activities. High profit was observed in hybrid rice and low profit was obtained in
conventional rice. Most of the farmers focused to grow hybrid rice due to high yield.
Keywords: Rice, performance, hybrid, conventional, varieties, Pakistan
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Maize intensification in major production regions of the world
1. Maize intensification in major production
regions of the world: Evidence from a
global multi-location study
T. Satyanarayana and Sudarshan Dutta (IPNI South Asia Program)
T. Scott Murrell and Luís Prochnow (IPNI Global Maize Program)
Kaushik Majumdar (IPNI Asia & Africa)
13th Asian Maize Conference and Expert Consultation on “Maize for Food, Feed,
Nutrition, and Environmental Security, Ludhiana, India, 8-10 October 2018
2. Global maize trends
• Steady increase of yield over time.
– Since the 1960s, yields have been
improving at a rate of 65 kg/ha/yr
(FAO, 2017).
• World’s total maize production
– increasing at a steady rate of 10
million M t/yr until 2004
– shifted to a steeper production of
31 M t/yr (FAO, 2017).
• Maize harvest area expansion
– increasing at a rate of 0.9 M ha/yr prior
to 2007, has now been increasing at
the more rapid pace of 4.7 M ha/yr.
3. Intensification of maize systems
• Ecological Intensification (EI) is
“...a production system that
satisfies the anticipated increase
in food demand while meeting
acceptable standards for
environmental quality”
(Cassman, 1999)
• Key elements of intensification
– Closing exploitable yield gap
– Improving soil quality
– Precision agriculture
Cassman, K.G. 1999. Proc. Natl. Acad. Sci. 96:5952-5959; Evans and Fischer. 1999. Crop Sci. 39:1544
4. 13 Scientists dedicated towards Global Maize Initiative at 20 research
centers in 9 Countries
IPNI Global Maize Initiative
Ecologically Intensifying Maize Based Cropping Systems across the World
6. Avenues for advancing intensification in
maize based systems
• Exploring Maize Intensification with the Global Yield Gap
Atlas
• Approaches for intensification management
– when yield gaps are narrow
– when yield gaps are wide
– increase Nutrient Use Efficiency while maintaining yield levels
– when maize is not the primary crop
– role of precision agriculture in closing maize yield gaps
• Educating Farmers and Crop Advisers about maize
intensification
7. Maize intensification with GYGA
• Estimates of yield
potential and yield gap
for maize in 42
countries.
• The Atlas identify
regions with greatest
potential for
sustainable maize
intensification.
• Maize yield gaps
– 85% in SSA and India
– 15% in USA and
Europe
The Global Yield Gap Atlas (www.yieldgap.org)
8. Maize intensification when yield gaps are wide,
example from India
• Combining 4R with optimal
planting time, planted
population, hybrid selection,
residue management, etc.
– Consistently higher yield (26%)
over FP
– A net return of US$1,080/ha
was obtained with EI, which
was 22% higher than FP
– PFPN was higher in EI (18.7)
than FP (17.1)
– AEN was also higher under EI
(35.7) than with FP (9.1)
9. Maize intensification when yield gaps are wide
• In Muguga, yield across all
treatments were >5 t/ha
– more that 500% higher than the
yield in smallholder farming
systems
– balanced nutrient management
increased grain yield over N+P
by 2-12%
– highlights the need to change the
blanket recommendations
– tailor fertilizer sources to account
for multiple nutrient deficiencies GMP results at Muguga in central Kenya (top)
and Kambiyamwe in eastern Kenya (bottom)
10. Year Observed yield Yw 85% Yw Yield gap
EI FP 0.85Yw-EI 0.85Yw-FP
2009 8.7 7.6 10.6 9.0 0.3 1.4
2010 11.3 11.6 14.2 12.1 0.8 0.5
2011 11.9 10.4 15.9 13.5 1.6 3.1
2012 12.5 12.6 15.7 13.3 0.8 0.7
2013 11.6 11.2 15.0 12.8 1.2 1.6
Mean 11.2 10.7 14.3 12.1 0.9 1.5
Yield data in t/ha; Yw=potential yield of maize based on rainfed conditions by using Hybrid Maize Model; 85% of Yw is
the maximum attainable yield (Source: Zhao and He, 2017)
Maize intensification to maintain yield
while increasing NUE An example from China
Treatment Fertilizer use, kg/ha N timing Hybrid Population/
ha
N P2O5 K2O
EI 180 70 90 3 splits Pioneer 335 65,000
FP 250 145 100 All basal Local variety 50,000
12. Opportunities for intensification approaches
when yield gaps are narrow
• At a rainfed site in Iowa, US
– strip-till maize and no-till soybean in EI over more intensive, full-
width conventional tillage in the FP
– reducing the overall N rate, additional split application
– EI produced comparable maize yields (10.65 t/ha) over
FP (11.02 t/ha)
– AEN improved in EI (35 kg/kg) over FP (25 kg/kg)
• In a clay loam soil at southern Russia
– EI for maize and other rotational crops
– increased maize yield by 9%, soybean yield by 25%, and
chickpea yield by 27%.
– resulted in greater overall system productivity
13. Precision Nutrient management in maize systems
Parameter Unit Effect of NE (NE – FFP)
India Indonesia Philippines
(n = 412) (n = 26) (n = 190)
Grain yield t/ha +1.27 *** +0.92 *** +1.10 ***
Fertilizer cost USD/ha –1 ns +16 ns +37 ***
Gross profit USD/ha +256 *** +234 *** +267 ***
*** significant at P<0.001; ns = not significant
14. Frequency Distribution of Grain Yield
0
2
4
6
8
10
0
2
4
6
8
10
4000
5000
6000
7000
8000
9000
10000
11000
12000
13000
14000
15000
16000
Frequency
Dry matter grain yield (kg DM ha-1)
EI
FP
Average = 8021
Average = 7041
15. Environmental Component of GMP
• Examining the “…minimizing adverse environmental
effects” part of the definition of ecological intensification
• Examined various efficiencies
– Nitrogen:
• Partial factor productivity
• Agronomic efficiency
• Recovery efficiency
– Nitrogen, Phosphorus, Potassium:
• Partial nutrient balance
16. How EI Affected Partial Factor
Productivity of Nitrogen
-50
0
50
100
150
200
250
-50 0 50 100 150 200 250
ChangeinEIgrainyield(%)
Change in N rate used in EI (%)
EI maintained PFP
EI reduced PFP
EI increased PFP
1:1Compared to FP:
PFP =
Grain yield
N applied
17. Frequency Distribution of Agronomic
Efficiency
0
2
4
6
8
10
0
2
4
6
8
10
5
10
15
20
25
30
35
40
45
Frequency
Agronomic efficiency
(kg ha-1 grain DM increase (kg ha-1 N applied)-1
EI
FP
Profitable use of N
Average = 19.0
Average = 19.7
18. How EI Affected Partial Nutrient Balance
of Phosphorus
1:1
0
1
2
3
4
5
6
0 1 2 3 4 5 6
EIPNB(kgP2O5ha-1removed
(kgP2O5ha-1applied)-1)
FP PNB (kg P2O5 ha-1 removed
(kg P2O5 ha-1 applied)-1)
EI reduced PNB
EI maintained PNB
EI increased PNB
PNB =
Removed
Applied
FP builds
fertility
FP depletes
fertility
EI builds
fertility
EI depletes
fertility
19. How EI Affected Partial Nutrient Balance
of Potassium
0
1
2
3
4
5
6
0 1 2 3 4 5 6
EIPNB(kgK2Oha-1removed
(kgK2Oha-1applied)-1)
FP PNB (kg K2O ha-1 removed
(kg K2O ha-1 applied)-1)
EI reduced PNB
EI maintained PNB
EI increased PNB
1:1
PNB =
Removed
Applied
FP builds
fertility
FP depletes
fertility
EI builds
fertility
EI depletes
fertility
20.
21. Questions addressed in the GMP
• IPNI’s mission is focused on the nutrient
management subset (4Rs) of practices in
cropping systems
– we fully appreciate how that subset is not only
interactive internally, but interacts with many other
factors of the production system and that those
other factors can markedly influence the
performance of nutrient inputs.
• How productive and efficient can maize systems become if
our best knowledge and technology are all brought to bear in
meeting sustainability objectives?
• How efficient and effective can nutrients and other inputs and
resources become?