This document discusses vitamin B12, also called cobalamin. It provides details about:
1. Vitamin B12 is a water-soluble vitamin that plays a key role in the functioning of the nervous system and formation of red blood cells. It is involved in DNA synthesis and metabolism.
2. Vitamin B12 is not synthesized by animals or plants, but rather by microorganisms. It is produced commercially through bacterial fermentation using microbes like Streptomyces and Propionibacterium.
3. The industrial production process involves growing the microbes in nutrient media containing sources of carbon, nitrogen, salts and cobalt. The fermentation yields 3.3-20 mg/L
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
streptomycin production, uses, disadvantages , medium, inoculum preparation, commercial production, harvest and recovery process, biosynthetic pathway from glucose to streptomycin, flow sheet of streptomycin production by submerged culture method, chemical structure of streptomycin,
which functional unit have antibiotic activity?
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
B12 metabolism..................................... and role of various proteins in b12 metabolism..... necessity of supplementation..........................................
streptomycin production, uses, disadvantages , medium, inoculum preparation, commercial production, harvest and recovery process, biosynthetic pathway from glucose to streptomycin, flow sheet of streptomycin production by submerged culture method, chemical structure of streptomycin,
which functional unit have antibiotic activity?
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
B12 metabolism..................................... and role of various proteins in b12 metabolism..... necessity of supplementation..........................................
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
Primary Metabolite- Vitamin B12 Structure and Sources, General Biosynthesis of Vitamin B12, Fermentation production by Propionibacteria and Pseudomonas, Isolation of Vitamin B12, Fermentation using other substrates, Production of Vitamin B12 derivatives, Antagonists of Vitamin B12
Primary methods of producing vitamin B12 include microbial fermentation using naturally occurring bacteria such as *Propionibacterium* and *Pseudomonas*. Usually used in industrial manufacturing, genetically modified strains of these bacteria increase output. The procedure begins with fermenting a medium high in nutrients, then uses centrifugation, filtration, and chromatography to extract and purify vitamin B12. The finished product is next formulated into meals that have been fortified and dietary supplements. Because vitamin B12 is so important to human health—in particular, to DNA synthesis, red blood cell generation, and brain function—efficient production of the vitamin is essential.
Vitamin B12 biosynthesis is restricted to microorganisms. Most of the steps in the
biosynthesis of vitamin B12 have been characterized in Pseudomonas denitrificans, Salmonella
typhimurium and Propionibacterium freudenreichii. Some authors have reported about the
requirement of more than 30 genes for the entire de novo biosynthesis of cobalamin, which
amounts to about 1 % of a typical bacterial genome. Two different biosynthetic routes for
vitamin B12 exist in nature:
• aerobic, or more precisely an oxygen-dependent pathway that is found in organisms like P.
denitrificans, and
• anaerobic, oxygen-independent pathway investigated in organisms like P. shermanii,
Salmonella typhimurium and Bacillus megaterium.
A broad module on industrial microbiology is summarized with pictures .It includes the production of vitamins,vaccine ,alcohol,vinegar,steroids,amino acids ,antibiotics .it also includes the general idea on history ,media,equipment,fermentation,procedure ,uses of industrial microbiology .The production of wine,beer and vinegar are mine core interest .Hope may help ....Thank you .
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT PRODUCTION OF CITRIC ACID , PENICILLIN, GLUTAMIC ACID , GRISIOFULVIN , VITAMIN B 12
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
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Monitor common gases, weather parameters, particulates.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
2. A vitamin is an organic compound and a vital nutrient that
an organism requires in limited amounts.
They are of great value in the growth and metabolism of the
living cells.
Vitamins are obtained with food, but a few are obtained by
other means ; humans can produce some vitamins from
precursors they consume while certain microorganism
produce vitamins too.
Thirteen vitamins are universally recognized at present,
vitamins are classified by their biological and chemical
activity.
Vitamins can be classified as “Fat soluble vitamins” and
“Water soluble vitamins”
4. Vitamin B12, also called Cobalamin, is a water-
soluble vitamin that has a key role in the normal functioning
of the brain and nervous system, and the formation of red
blood cells.
It is involved in the metabolism of every cell of the human
body, especially affecting DNA synthesis, fatty
acid and amino acid metabolism.
It is synthesized only by microorganisms and not by animals
(including humans) and plants.
People with B12 deficiency may eventually develop
Pernicious anemia.
It is the largest and most structurally complicated vitamin
and can be produced industrially only through bacterial
fermentation-synthesis.
5.
6.
7. Cyanocobalamin, is the industrially produced stable
Cobalamin form which is not found in nature.
Vitamin B12 is entirely produced on a commercial basis by
the fermentation. It is usually manufactured by submerged
culture process. Such a fermentation process is completed in
3-5 days.
Most of the B12 fermentation processes use glucose as a
carbon source.
The microorganisms that maybe employed in the industrial
production process are :
i. Streptomyces griseus
ii. Streptomyces olivaceus
iii. Bacillus megaterium
iv. Bacillus coagulans
v. Pseudomonas denitrificans
vi. Propionibacterium freudenreichii
vii. Propinibacteriun shermanii
8. Step 1 • Formulation of the medium
Step 2 • Sterilization of the medium
Step 3 • Making starter culture
Step 4 • Anaerobic fermentation
Step 5
•Aerobic fermentation
Step 6
•Recovery
9. Production by Streptomyces olivaceus yields about 3.3mg / L
of vitamin B12.
Process :
A. Preparation Of Inoculum :
Pure slant culture of S. olivaceus is inoculated in 100-250ml of
inoculum medium, contained in Erlenmeyer flask.
Seeded flask is incubated on platform of a mechanical shaker to
aerate the system.
This flask culture is then subsequently used to inoculate larger
inoculum tanks.
(2 or 3 successive transfers are made to obtain required amount
of inoculum cultures.)
10.
11. Media used in preparation of inoculum is Bennett’s agar.
Component Amount
(g/L)
Yeast extract 1.0
Beef extract 1.0
N-Z-Amine A
(Enzymatic hydrolase of
casein)
2.0
Glucose 10.0
Agar 15.0
D/W 1000 mL
pH 7.3
12. B. Production Medium :
Consist of carbohydrate, proteinaceous material, and source
of cobalt and other salts.
It is necessary to add cobalt to the medium for maximum
yield of cobalamin.
Cyanide is added for conversion of other cobalamins to
vitamin B12.
Component Amount (%)
Distiller’s Solubles 4.0
Dextrose 0.5 - 1
CaCO3 0.5
COCL2.6H2O 1.5 – 10 ppm
pH 7
13. C. Sterilization of the medium :
Sterilization can be done batchwise of continuously.
Batch – medium heated at 250°F for 1 hour.
Continuous – 330°F for 13 min by mixing with live steam.
D. Temperature , pH , Aeration and Agitation :
• Temperature : A temperature of 80°F in production tank is
satisfactory during fermentation.
• pH : At starting of process pH falls due to rapid
consumption of sugar, then rises after 2 to 4 due to lysis of
mycelium. pH 5 is maintained with H2SO4 and reducing
agent Na2SO4.
• Aeration and Agitation : Optimum rate of aeration is 0.5
volume air/volume medium/min.
14. E. Antifoam agent , Prevention of contamination :
Antifoam agent : Defoaming agents like soya bean oil , corn
oil, lard oil and silicones can be used.
Prevention of contamination : Essential to maintain sterility,
contamination results in reduced yields, equipments must be
sterile and all transfers are carried out under aseptic
conditions.
F. Recovery :
During fermentation, most of cobalamin is associated with
the mycelium; boiling mixture at pH 5 liberates the
cobalamin quantitatively from mycelium.
Broth containing cobalamin is subjected to further process
to obtain crystalline B12.
15. Filtration of broth to remove mycelium.
Filtered broth is treated with cyanide to bring conversion of cobalamin
to cyanocobalamin.
Adsorption of cyanocobalamin from the solution is done by passing it
through adsorbing agents packed in a column.
Cyanocobalamin is then eluted from the adsorbent by the use of an
aqueous solution of organic bases or solutions of Na-Cyanide and Na-
thiocyanate.
Extraction is carried out by countercurrent distribution between cresol,
amylphenol, or benzyl alcohol and water or a single extraction into an
organic solvent (e.g. Phenol) is carried out.
Chromatography on alumina and final crystallization completes the
process.
16. Production by Propionibacterium freudenreichii yields about
20mg/L of vitamin B12.
A. Production media : glucose , corn- steep , betaine , & cobalt.
Betaine -0.5 %
Cobalt – 5µg./ml (excess cause reduced cobalamin
formation)
B. pH -7.5
C. Temperature – 30°C
17. D. Fermentation : It involves to cycles; anaerobic fermentation
cycle of 70 hours and Aerobic fermentation cycle of 50 hours.
Anaerobic fermentation :
Formation of cobinamide occurs.
The pH falls from 7.5 to 6.5 and then rises upto 8.5.
Necessary to add 0.1% of 5, 6 – dimethyl benziminazole to the
production medium.
Aerobic fermentation :
Nucleotide formation takes place.
This nucleotide then links with cobinamide to give cobalamin.
18.
19. Species Medium Aeration Temp.
(°C)
Time
(hour)
Yield
(mg/L)
B.megaterium Molasses ,
mineral
salts, cobalt
Aerobic 30 18 0.45
P.
shermanii
Glucose ,
corn- steep,
ammonia ,
cobalt
pH 7.0
Anaerobic
(3 days),
aerobic (4
days)
30 150 23
B.
coagulants
Citric acid ,
triethanolam
ine , corn –
steep ,
cobalt.
Aerobic 55 18 6.0