Cells are the basic unit of life and can be prokaryotic or eukaryotic. Cell culture involves growing living cells in a controlled, in vitro environment with optimal temperature, pH, media, and isolation from contamination. There are different types of cell cultures including cell, tissue, and organ cultures. Tumors are uncontrolled cell growth that can be caused by genetic, environmental, or other regulatory body factors. Tumor cell cultures involve sampling tumor cells, initially establishing them in culture, and characterizing tumor cells through methods like DNA profiling, isoenzyme analysis, and studying morphological and cultural features.

3. Cell..??
• Basic unit of Life.
• Prokaryotic cells
• Eukaryotic cells
• Functions of cells.?
• Organs.?
• Organism.?

4. CELL CULTURE
• Culture of living cells in the in vitro condition with
suitable growth media.
• Should need
• Optimum Temperature,
• pH,
• Proper media,
• Incubator
• Out of Microbial contamination , etc.

5. Revision..
• Types of Culture.?
• Cell culture,
• Tissue culture,
• Organ culture
• What is Callus.?
• Dedifferentiation..?
• Differentiation..?
• Redifferentiation.?
• Uni potent.?
• Multi potent.?
• Primary Vs. Secondary culture (Cell lines)..?
• Sensence.?

6. Tumor
• Uncontrolled growth of normal cells.
• Why.?
• Gentical,
• Environmental,
• Other Body regulatory factors.

8. Tumor Cell Culture
• Primary tumor cell culture.?
• Sampling of tumor cells.
• Initial establishment.
• Disaggregation.
• Cancer cell lines.?
• Characterization of Tumor cells.
• Common Problems with Tumor cell culture.

9. Primary tumor cell culture.?
• Sampling of tumor cells.
• Cell banks.
• World federation for Culture – Having 469
centers in world level.
• Isolation of Tumors from Animals.
• Initial Establishment.
• Transfer of Fragment to hood.
• Viable cells. (Remove Necrotic cells)
• PBS wash (To remove Blood)

10. Disaggregation
• Mechanical method &
• Enzymatical method.

11. Mechanical
• Scalpels,
• Pulverize,
• Avoid cluster of cells,
• Centrifuge,
• Inoculate.

12. Enzymatical
• Trypsin,
• Collagenase,
• Elastase,
• Dispase,
• Hyaluronidase,
• Papin.

13. CHARACTERIZATION OF
TUMOR CELLS
• While establishing new cell lines, it is
required.
• Origin of cell lines.
• 3 Methods :
• Access uniqueness
• Specific origin
• General features

14. 1.Access uniqueness
• Using Molecular biological experiments.
• Based on DNA profiles.
• DNA finger printing,
• DNA profiling,

15. 2. Species of Origin
• Iso enzyme analysis
• Cytogenetic studies,
• Antibody detection methods (IFA),
• PCR studies.

16. 3.General features
• Morphological studies,
• Cultural characters,
• Cell cycle characters,
• Cell – Cell communication.

18. My References
1) Methods in Molecular medicine – Cancer
Cell culture – Methods & Protocols by
Simon P.Langdon, Humana press.
2) Primary cell culture guide – Tips &
Techniques – ATCC.