This document discusses cell culture and cell lines. It begins by defining cell lines as permanently established cell cultures that can proliferate indefinitely with fresh medium and space. It then describes the process of establishing cell lines from primary cultures through multiple subculturings. The document outlines different types of tissue cultures including primary, finite, and continuous cell lines. It provides examples of cell lines derived from normal, transformed, and stem cells from both human and animal sources. Finally, it briefly mentions some applications of established cell lines.

1. CELL CULTURE AND ITS
MAINTENANCE:
MADE BY:
VIPIN KUMAR SHUKLA
DEPARTMENT OF BIOTECHNOLOGY.
HIMT GROUP OF INSTITUTIONS , GREATER NOIDA UTTAR PRADESH

2. CONTENTS:
• CELL LINES
• TYPES OF TISSUE CULTURES
• PRIMARY TISSUE CULTURES
• CONTINOUS CELL LINES
• CELL TYPES
• HUMAN CELL LINES
• ANIMAL CELL LINES
• APPLICATIONS

3. CELL LINES:
• It is defined as permanently established cell culture
that will proliferated indefinitely given appropriate
fresh medium and space.
• Cell line defined as the culture developed from single
cell and therefore it consisting of cell with a uniform
genetic make up.
• Cell lines shows a presence of cell linkage either
similar or distinct.
• In this culture stem cells are generally used.

4. CONTD…..
• At first primary culture is done, Later sub-Culturing is
done, then later the primary culture becomes the cell
line may be propagated and sub cultured multiple
times.
• There are some species which may be used for rising
cell lines such as: Rodents etc while other species do
not.
• Cell lines have been produced from avian tissues and
also the development of cell lines from human tissue is
difficult.

5. TYPES OF TISSUE CULTURE:
PRIMARY CONTINOUS
FINITE CELL
LINES
IN-DEFINATE
Normal cells
cultured without any
change in their
division rate.
Single type roughly, thirty times
of division enhanced by growth.
It is near the same as
finite but the cells here
can divide indefinitely
by transformation into
tumor cells, called Cell
Lines.
PRIMARY CONTINOUS

6. CELL LINE:
• CELL LINE
NORMAL
TRANSFORMED
STEM CELLS
It is a single cell
type and taken
from tumor cell
and cultured.
There will be genetic change
I normal cell which become
tumor cell.
They are called as
master cells that
generated other
differentiated cell
types.
NORMAL
CELLS
TRANSFORMED
CELLS
STEM CELLS

7. PRIMARY CULTURE:
• Primary culture is defined as a culture were cells are
removed enzymatically and surgically from an organism
and later placed into a suitable culture medium that will
attach and grow are called as primary culture.
• These primary culture have a finite life span.
• These cultures contain a heterogeneous population of
cells.

8. Contd…..
• Sub culturing of primary cells leads to the generation
of cell lines.
• Cell lines have limited life span, they passage several
times before they become senescent.
• Cells such as Macrophages and neurons do not divide
in-vitro can be used as primary cultures.

9. CONTINOUS CELL LINES:
• Most cell lines grow for a limited number of
generations after which they cease.
• Cell lines which either occur spontaneously or
induced virally are chemically transformed into
continuous cell lines.

10. CHARACTERSTICS OF
CONTINOUS CELL LINES:
• These are more rounded, les adherent, with a higher
nucleus/ cytoplasmic ratio.
• Fast growth.
• Grow in more in suspension conditions.
• Ability to grow up to higher cell density.
• Stop expressing tissue specific genes.

11. CELL TYPES:
• On the basis of morphology ( Appearance and Shape) or
on their functional characteristics. They are divided into
three.
• Epithelial like: Attached to a substrate and appears
flattened and polygonal in shape.
• Lymphoblast like: Cells do not attach, remain in
suspension with a spherical shape.
• Fibroblast like: Cells attached to a substrates, appear
elongated and bipolar.

12. HOW CAN CELL LINE DERIVED:

13. ONE STEM LINE ONE GROWING
EMBRYO:

14. FINITE AND CONTINOUS CELL
LINES:

15. Contd…..

16. Contd…..

17. Animal cell lines and its products:

18. Applications of cell lines: