Somatic cell nuclear transfer (SCNT) is a cloning method that involves removing the nucleus from a somatic cell and placing it in an egg cell. The key steps are removing the nucleus from the organism being cloned and an egg cell, placing the organism's nucleus into the egg cell, stimulating the egg cell with a shock, and allowing it to divide. SCNT is mostly used for animal cloning and produced Dolly the sheep, but has limitations as it requires many attempts and does not perfectly transfer all DNA.

2. Somatic Cell Nuclear Transfer
Josh Reichman

4. What is it?
Somatic cell nuclear transfer (SCNT) is a
method of cloning an organism that involves
removing the nucleus from a somatic cell and
putting it in an egg cell.

5. What is it?
Somatic cell nuclear transfer (SCNT) is a
method of cloning an organism that involves
removing the nucleus from a somatic cell and
putting it in an egg cell.

8. The Steps
1. Nucleus is taken out of the
organism that is being
cloned.
2. Nucleus of an egg cell is
removed.
3. The organism's nucleus is
placed into the egg cell.
4. The new nucleus get
reprogrammed as if it were
an egg cell.
5. The egg cell is stimulated
with a shock
6. The egg cell starts to divide.

9. The Steps
1. Nucleus is taken out of the
organism that is being
cloned.
2. Nucleus of an egg cell is
removed.
3. The organism's nucleus is
placed into the egg cell.
4. The new nucleus get
reprogrammed as if it were
an egg cell.
5. The egg cell is stimulated
with a shock
6. The egg cell starts to divide.

10. The Steps

13. Notable Uses
Somatic cell nuclear transfer is mostly
used in animal cloning. One of the most
famous clones, Dolly the Sheep, used
this method of cloning.

14. Notable Uses
Somatic cell nuclear transfer is mostly
used in animal cloning. One of the most
famous clones, Dolly the Sheep, used
this method of cloning.

17. Limitations
• It takes a lot of tries to get a successful
clone
• The procedure is manual
• Some of the wanted DNA is not transferred
• Some of the original egg's DNA is left
behind
• Results in a close, but not perfect clone

20. Controversy
1. Cloning of humans
2. The requirement of eggs
from a female

23. Good and Bad
Good Bad
Simple process It's not perfected
Used in many Not all DNA is
experiments transferred
Can be done reproductively or Not all egg DNA is
therapeutically
removed
Could be used to Requires lots of
cure diseases tries

26. Gallery

27. Thank you for your
time