This document discusses various tests used to evaluate facial nerve function including physical examination, House Brackmann grading scale, topodiagnostic tests, and electrodiagnostic tests. Physical examination evaluates features like facial asymmetry, eyebrow position, and ability to close eyes. Electrodiagnostic tests like electroneuronography, electromyography, and acoustic reflex testing can help localize facial nerve lesions and assess prognosis for recovery. Together these evaluation methods provide information on the severity and location of facial nerve damage.
Spaces of middle ear and their surgical importanceDr Soumya Singh
one of the imp topics in ENT that should be understood very thoroughly if u want to pursue as an otologist.I tried to simplify the topic with simple diagrams and models for better understanding .
This is a presentation I used for my seminar on 'Phonosurgery' on 4th November, 2015. I hope they are useful to you. Constructive as well as Destructive criticism welcomed.
Spaces of middle ear and their surgical importanceDr Soumya Singh
one of the imp topics in ENT that should be understood very thoroughly if u want to pursue as an otologist.I tried to simplify the topic with simple diagrams and models for better understanding .
This is a presentation I used for my seminar on 'Phonosurgery' on 4th November, 2015. I hope they are useful to you. Constructive as well as Destructive criticism welcomed.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
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The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
2. Facial Nerve Test
Detect facial nerve lesion
Measure severity
Localise site of lesion
Assess prognosis for recovery
Assist in decisions
Detect and avoid surgical injury*
3. PHYSICAL
EXAMINATION
Facial assymetry*
Eye brow drop
Loss of forehead *and nasolabial fold
Uncontrolled tears
Inability to close the eyes*
Drooling of corner of mouth
Deviation of angle of mouth to opposite
side*
5. TOPODIAGNOSTIC
TESTS
Lesion below the point at which particular
branch leave the facial nerve trunk will
spare the function subserved by that
branch
Detect only most proximal lesions
Do not give information about precise
level of lesions
7. LACHRYMAL
FUNCTION
Schirmer test
Abnormal if affected side show less than
50% lachrymation compared to normal
side
or
Sum of the length of wetted filter paper
for both eyes is <25mm
Modified schirmer test?
8. STAPEDIAL REFLEX
ABSENT REFLEX OR REFLEX LAST
LESS THAN ½ OF AMPLITUDE OF
NORMAL SIDE IS ABNORMAL
USEFUL TO PREDICT RECOVERY
+VE REFLEX <2 WEEKS OF INJURY
FULL RECOVERY OF NERVE WITHIN
12 WEEKS
9. TASTE FUNCTION
Using pellets with Nacl or
electrogustometry
Two sides of tongue has similar
threshold electric stimulation –normal
Abnormal EGM response-no response
on affected side or threshold difference
is >20dB uA
10. SALVARY FLOW TEST
<45% flow compared to normal side on
using 6%citric acid is abnormal
<25%flow compared to normal side is
indication for surgery
Submandibular scintigraphy-peak count
density,washout ratio<0.8 –incomplete
recovery in long term
Submandibular saliva pH <6.1 -poor
recovery
11. ELECTRODIAGNOSTIC
TESTS
Apply electric stimulation and measure
electromyographic response
Identify patients with degeneration which is
an indication for decompression surgery
12. NERVE EXCITABILITY
TEST
Difference of 2 to 3.5 mA in threshold
between both sides-reliable sign of
severe degeneration
Increasing pulses of electrical
stimulation given
13. MAXIMUM
STIMULATION
TEST
Supramaximal stimulus applied on
unaffected side until no further increase
in response is obtained and same
amount of stimuli applied to affected
side and response is measured as
0%,25%,50%,100%
No response is an indication for
decompression
14. ELECTRONEURONOGRA
PHY
Same as nerve excitability test but here
1 electrode at stylomastoid foramen and
other elctrode at nasolabial groove
Normal response-difference in amplitude
between 2 sides is within 3%
Quantify % of fibres degenerated
Even if facial movement is normal and
ENoG is abnormal degeneration is
present
15. ELECTROMYOGRAPH
Y
Measure spontaneous and voluntary
muscle potentials induced into muscle
Active –stimulus applied and cMAP
measured
Passive-spontaneous cMAP measured
Used for intraoperative facial nerve
monitoring
Most accurate to detect defective
healing
16. ACOUSTIC REFLEX
EVOKED
POTENTIALS
Sound stimus applied and potentials
recorded in scalp electrodes
Impulses after 12-15ms latency is d/t
facial motor pathway activation
Persist even after anaesthesia.
Intraop facial nerve monitoring
18. BLINK REFLEX
Electrical or mechanical stimulation of
supraorbital br of trigeminal nerve elicits
reflex contraction of orbicularis oculi
Intra op facial nerve monitoring
20. TRANSCRANIAL
EXCITATION OF FACIAL
MOTOR CORTEX
Electrodes placed at c/l facial motor cortex
and response measured at muscle supplied
by it as MEP.
Intraop facial nerve monitoring
Entire facial nerve tract is tested