Sterilization is a process that kills all microorganisms through various means like heat, chemicals, irradiation, etc. Dry heat sterilization uses high temperatures without moisture to destroy microbes. Common dry heat methods include hot air ovens, flaming, and incineration. Hot air ovens circulate hot air above 170°C for at least 60 minutes to ensure sterilization through protein denaturation and cell membrane disruption. Flaming uses the flame of a Bunsen burner to heat items red hot above 250°C, while incineration reaches temperatures over 870°C to sterilize through complete burning. Dry heat requires higher temperatures and longer times than wet heat sterilization methods.
it is related with medical laboratory instrumentation and explains in very good way that what is hot air oven and its principle, working and all about it
Terminology
Introduction of Disinfectants
Classification of Disinfectants
Mode of action of Disinfectants
Factors affecting Disinfection
Evaluation of Anti-microbial agents and Disinfectants
it is related with medical laboratory instrumentation and explains in very good way that what is hot air oven and its principle, working and all about it
Terminology
Introduction of Disinfectants
Classification of Disinfectants
Mode of action of Disinfectants
Factors affecting Disinfection
Evaluation of Anti-microbial agents and Disinfectants
Sterilization (or sterilisation) referring to any process that eliminates (removes) or kills (deactivates) all forms of life and other biological agents (such as prions, as well as viruses which some do not consider to be alive but are biological pathogens nonetheless), including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes the basic knowledge on sterilization and tests for sterility.
Autoclave, types of autoclave, horizontal autoclave, vertical autoclave, vacuum type autoclave, pressure cooker type autoclave. their purpose, precaution, etc....
This ppt includes all the key points of process of sterilization and its different techniques like physical,chemical,thermal,etc. sterilization is very important topic to go through during education as well as during practice to maintain a nice infection free environment of your health care office or clinic.
Sterilization Process and methods of sterilizationShahnawaz Ahmad
Presented by Shahnawaz Ahmad.
Various methods of sterilization used in microbiology or other field.
contents ;
terms used in sterilization
types of sterilization
physical method
chemical method
radiation
filtration
gaseous.
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes principle and working of hot air oven and autoclave.
Presentation showing various methods used for confirmation of sterilization processes. This includes various methods used for confirmation of sterilization done by filtration sterilization, Thermal sterilization, radiation sterilization, gaseous sterilization etc.
Classification and mode of action of disinfectants PHARMACEUTICAL MICROBIOLOG...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-III Classification and mode of action of disinfectants. DISINFECTANT
Definition: Ideal properties of disinfectants: CLASSIFICATION OF DISINFECTANTS: Based on consistency 1. Liquid (E.g., Alcohols, Phenols) 2.Gaseous (Formaldehyde vapor, Ethylene oxide). Based on spectrum of activity 1. High level disinfectant
2. Intermediate level disinfectant
3. Low level disinfectant .Based on mechanism of action: 1.Action on membrane2.Denaturation of cellular proteins 3.Damage to nucleic acids 4.Oxidation of essential sulfhydryl groups of enzymes 5.Alkylation of amino-, carboxyl- and hydroxyl group. MODE OF ACTION AND APPICATION OF DISINFECTANT
Acid and alkalies
Halogens
Heavy metals
Phenols and its derivatives
Alcohol
Aldehydes
Dyes:
Quaternary ammonium compounds
Detergents and soaps.
When fresh liquid medium is inoculated with a given number of bacteria and incubated for sufficient period of time, it gives a characteristic growth pattern of bacteria.
If the bacterial population is measured periodically and log of number of viable bacteria is plotted in a graph against time, it gives a characteristic growth curve which is known as growth curve or growth cycle.
Sterilization (or sterilisation) referring to any process that eliminates (removes) or kills (deactivates) all forms of life and other biological agents (such as prions, as well as viruses which some do not consider to be alive but are biological pathogens nonetheless), including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes the basic knowledge on sterilization and tests for sterility.
Autoclave, types of autoclave, horizontal autoclave, vertical autoclave, vacuum type autoclave, pressure cooker type autoclave. their purpose, precaution, etc....
This ppt includes all the key points of process of sterilization and its different techniques like physical,chemical,thermal,etc. sterilization is very important topic to go through during education as well as during practice to maintain a nice infection free environment of your health care office or clinic.
Sterilization Process and methods of sterilizationShahnawaz Ahmad
Presented by Shahnawaz Ahmad.
Various methods of sterilization used in microbiology or other field.
contents ;
terms used in sterilization
types of sterilization
physical method
chemical method
radiation
filtration
gaseous.
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes principle and working of hot air oven and autoclave.
Presentation showing various methods used for confirmation of sterilization processes. This includes various methods used for confirmation of sterilization done by filtration sterilization, Thermal sterilization, radiation sterilization, gaseous sterilization etc.
Classification and mode of action of disinfectants PHARMACEUTICAL MICROBIOLOG...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-III Classification and mode of action of disinfectants. DISINFECTANT
Definition: Ideal properties of disinfectants: CLASSIFICATION OF DISINFECTANTS: Based on consistency 1. Liquid (E.g., Alcohols, Phenols) 2.Gaseous (Formaldehyde vapor, Ethylene oxide). Based on spectrum of activity 1. High level disinfectant
2. Intermediate level disinfectant
3. Low level disinfectant .Based on mechanism of action: 1.Action on membrane2.Denaturation of cellular proteins 3.Damage to nucleic acids 4.Oxidation of essential sulfhydryl groups of enzymes 5.Alkylation of amino-, carboxyl- and hydroxyl group. MODE OF ACTION AND APPICATION OF DISINFECTANT
Acid and alkalies
Halogens
Heavy metals
Phenols and its derivatives
Alcohol
Aldehydes
Dyes:
Quaternary ammonium compounds
Detergents and soaps.
When fresh liquid medium is inoculated with a given number of bacteria and incubated for sufficient period of time, it gives a characteristic growth pattern of bacteria.
If the bacterial population is measured periodically and log of number of viable bacteria is plotted in a graph against time, it gives a characteristic growth curve which is known as growth curve or growth cycle.
Sterilization
It is defined as the process by which an article, surface or medium is freed of all living microorganisms either in vegetative or spore state.
Disinfection
It is destruction or removal of all pathogenic organisms or organisms capable of producing infections but not necessarily spores.
There have many methods of sterilization.,there have also sterilization with aseptic techniques for help to education. Easy to learn about of sterilization, and how to sterile easy to learn way. So i hope you'll enjoy and learn easily and to read about this slide.
share - Lions, tigers, AI and health misinformation, oh my!.pptxTina Purnat
• Pitfalls and pivots needed to use AI effectively in public health
• Evidence-based strategies to address health misinformation effectively
• Building trust with communities online and offline
• Equipping health professionals to address questions, concerns and health misinformation
• Assessing risk and mitigating harm from adverse health narratives in communities, health workforce and health system
DISSERTATION on NEW DRUG DISCOVERY AND DEVELOPMENT STAGES OF DRUG DISCOVERYNEHA GUPTA
The process of drug discovery and development is a complex and multi-step endeavor aimed at bringing new pharmaceutical drugs to market. It begins with identifying and validating a biological target, such as a protein, gene, or RNA, that is associated with a disease. This step involves understanding the target's role in the disease and confirming that modulating it can have therapeutic effects. The next stage, hit identification, employs high-throughput screening (HTS) and other methods to find compounds that interact with the target. Computational techniques may also be used to identify potential hits from large compound libraries.
Following hit identification, the hits are optimized to improve their efficacy, selectivity, and pharmacokinetic properties, resulting in lead compounds. These leads undergo further refinement to enhance their potency, reduce toxicity, and improve drug-like characteristics, creating drug candidates suitable for preclinical testing. In the preclinical development phase, drug candidates are tested in vitro (in cell cultures) and in vivo (in animal models) to evaluate their safety, efficacy, pharmacokinetics, and pharmacodynamics. Toxicology studies are conducted to assess potential risks.
Before clinical trials can begin, an Investigational New Drug (IND) application must be submitted to regulatory authorities. This application includes data from preclinical studies and plans for clinical trials. Clinical development involves human trials in three phases: Phase I tests the drug's safety and dosage in a small group of healthy volunteers, Phase II assesses the drug's efficacy and side effects in a larger group of patients with the target disease, and Phase III confirms the drug's efficacy and monitors adverse reactions in a large population, often compared to existing treatments.
After successful clinical trials, a New Drug Application (NDA) is submitted to regulatory authorities for approval, including all data from preclinical and clinical studies, as well as proposed labeling and manufacturing information. Regulatory authorities then review the NDA to ensure the drug is safe, effective, and of high quality, potentially requiring additional studies. Finally, after a drug is approved and marketed, it undergoes post-marketing surveillance, which includes continuous monitoring for long-term safety and effectiveness, pharmacovigilance, and reporting of any adverse effects.
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
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Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
1. An Assignment on
Sterilization by
Dry Heat
Submitted by
Roll:-14308
Reg:-1086
Date of Submission:-18/03/21
Submitted to
Mr Mohammad Kamal Hossain
Assistant professor
Department of Pharmacy,
University of Science and Technology
Chittagong (USTC)
Department of Pharmacy
Course Name: Applied Microbiology
Course No:-PHR-308
2. Sterilization refers to any process that removes, kills, or deactivates all forms
of life (in particular referring to microorganisms such
as fungi, bacteria, spores, unicellular eukaryotic organisms such as Plasmodium,
etc.) and other biological agents like prions present in a specific surface, object or
fluid, for example food or biological culture media. Sterilization can be achieved
through various means, including heat, chemicals, irradiation, high pressure,
and filtration. Sterilization is distinct from disinfection, sanitization,
and pasteurization, in that those methods reduce rather than eliminate all forms of
life and biological agents present. After sterilization, an object is referred to as
being sterile or aseptic.
Importance of sterilization:-
To prevent contamination in sterile products
To prevent transmission of pathogenic microorganisms which are
responsible for causing disease in plants, animals and human beings
To prevent decomposition and spoilage of food and food products
To prevent the contamination of unwanted microbes in pure cultures
and other microbiology experiments performed for research studies
To prevent unwanted microbial contamination in antibiotic,
enzyme, vitamins, fermentation and other industries process
To prevent contamination in aseptic areas/instruments which are used for
the preparation of sterile dosage forms and sterility testing.
Introduction
4. Dry Heat:-
Microorganisms are capable of causing infection are constantly present in
the external environment and on the human body.
Microorganisms are responsible for contamination and infection.
The aim of sterilization is to remove or destroy the microorganisms from
materials or from surfaces.
Denaturation of proteins
Interference with protein synthesis
Interruption of DNA synthesis
Oxidative damage of cell
Disruption of cell membranes
Dry Heat
Flaming Incinaration Hot Air Oven
Why we need Sterilization
How can microorganisms be killed
5. Fig;-Protein Denaturation
Contact time
Physico-chemical environment (e.g. pH)
Presence of organic material
Temperature
Type of microorganism
Number of microorganisms
Material composition
• Heat is the most reliable and rapid method of sterilization
Mechanism:
Protein denaturation
oxidative damage
Factors that influence efficacy of disinfection/sterilization
DRY HEAT STERILIZATION
6. Toxic effect of elevated levels of electrolytes.
Time required for sterilization is inversely proportional to the temperature
of exposure. This can be expressed as thermal death time, which is the
minimum time required to kill a suspension of microorganisms at a
temperature and specific conditions.
• Hot air ovens are electrical devices used in sterilization.
• The oven uses dry heat to sterilize articles.
• Generally, they can be operated from 50 to 300 C (122 to 572 F) .
• There is a thermostat controlling the temperature.
• This is the most widely used method of sterilization by dry heat.
• Items:glassware, forceps, scissors, scalpels, all-glass syringes,
swabs, liquid paraffin, dusting powder, fats, grease.
• (Materials should be properly arranged to allow free circulation of air)
Hot Air Oven
8. Component of Hot Air Oven:-
An insulated chamber surrounded by an outer case containing electric
heters.
A fan to ensure even distribution of air
Shelves
Thermocouples
Temperature
Door locking controls.
Precautions:
Should not be overloaded.
Arranged in a manner which allows free circulation of air.
Material to be sterilized should be perfectly dry.
Test tubes, flasks etc. should be fitted with cotton plugs.
Paper wrapping of the items should be done.
Rubber materials and inflammable materials should not be kept
inside.
The oven must be allowed to cool for two hours before opening, since
glassware may crack by sudden cooling.
Sterilization control:
Effectiveness of sterilization can be monitored by:
Biological indicators: — Paper strips with 106 Spores of Clostridium tetani
or Bacillus subtilis placed with other material. Later culture the strips in
thioglycollate broth at 370C for 5 days. Growth indicates failure
of sterilization.
Thermocouples: records the temperature by a potentiometer.
Browne's tube: contains a heat sensitive dye which turns green after being
exposed to 1600C for 60 minutes or 1500C for 115 minutes.
9. Advantages:-
• They do not require water and there is not much pressure build up within
the oven, unlike an autoclave, making them safer to work with.
• Suitable and easy to be use in a laboratory environment.
• They are much smaller than autoclaves but can still be as effective.
Disadvantages:
• long heating time, high temperature.
• As they use dry heat instead of moist heat, some organisms like prions, may
not be killed by them every time.
Items are held in the flame of Bunsen burner either for long time or short
time
longer time exposure in flame till they become red hot(250-3000C)
done for inoculating wires or loops, tips of forceps, etc.
Shorter period of time without allowing the items to become red hot: done
for fragile items ,e.g. glass slides, mouth of test tubes and flask.
Temperature (c) Time(in minutes)
170 60
160 120
150 150
140 180
Flamming
10. Fig:-Flamming
It is used for disposal of biomedical waste materials.
Materials are reduced to ash by burning.
It burns( sterilize) the anatomical waste , animal carcasses
,pathological waste and contaminated cloths by providing a very high
temperature(870- 1,2000C).
Incinaration
11. Fig:- Incinaration
:
Sterilization is a process or killing all microorganisms (including,spores) on
or in a material or object.
The factorsüat determine the type of sterilization or disinfecting process to
be used include time, temperature ,stage of growth of the organism ,nature
of the medium in which the organism is suspended (air, gas ,liquid) and the
number of organism present.
Sterilization and disinfection can be achieved by using heat, filtration,
chemical or radiation etc.
Overall, heat is the best means of sterilization, but other methods are used
for heat labile objects.
Summery
12. Dry heat requires more time than wet heat to kill organisms ,boiling kills
most vegetative cells but not bacterial spores & pressure cookers
&autoclaves achieve sterilization
Remington. The science and practice of pharmacy, 21st edition volume-1,
Page no.776-801.
Ananthanarayan ,Paniker's. Textbook of microbiology, (8th edition) page
no.30-38.
Tortora, Microbiology an introduction. (9th edition) page no. 188-197.
Purohit S S, Microbiology fundamentals and applications. (6th
edition) page no.354-366.
Michael J. Pelzar, Microbiology.(5th
edition).McGraw Hill, page no.474-
491.
Hugo and Russell 's, Pharmaceutical Microbiology. page no.336-345.
Dr. R. C. Dubey, Dr. D. K. Maheshwari, A textbook of microbiology. page
no. 110.546-549.
References