Sterilization and Disinfection
Sterilization kills all microorganisms including bacterial spores, while disinfection kills most pathogens excluding spores. Dry heat sterilizes through protein denaturation and moisture damage, while moist heat is more effective through coagulation and protein denaturation. Autoclaving at 121°C for 15 minutes is the most effective sterilization method. Membrane filtration below 0.45 μm is used to remove microbes from heat-labile liquids. Disinfectants like alcohol, aldehydes, phenol, hydrogen peroxide, ethylene oxide and halogens are used to disinfect surfaces, with alcohols and aldehydes being
Brief and easily understandable description on measles along with images for undergraduate students. this presentation would help in picturising what measles is.
Brief and easily understandable description on measles along with images for undergraduate students. this presentation would help in picturising what measles is.
this power point is useful to understand the theorical concept of a sterilization & disinfection ,autoclave for nursing students......hope it will be useful for you.
he culture media are classified in many different ways: Based on the physical state Liquid media Solid media Semisolid media Based on the presence or absence of oxygen Anaerobic media Aerobic media Based on nutritional factors Simple media Synthetic media Complex
types of sterilization,heat,cold,boiling,autoclave,hot air oven,disinfectant................................................................................................................................................................................................................................
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...
Sterilization and disinfection
1. Sterilization and Disinfection
Sterilization is defined as the process where all the living microorganisms, including bacterial spores are killed.
Disinfection is the process of elimination of most pathogenic microorganisms (excluding bacterial spores) on
inanimate objects.
Action of heat:
Dry heat acts by protein denaturation,oxidative damage and toxic effects of elevated levels of electrolytes. The
moist heat acts by coagulation and denaturation of proteins. Moist heat is superior to dry heat in action. Temperature
required to kill microbe by dry heat is more than the moist heat.
Thermal death time is the minimum time required to kill a suspension oforganisms at a predetermined temperature
in a specified environment.
Dry heat:
Red heat: Articles such as bacteriological loops, straight wires, tips of forceps and searing spatulas are sterilized by
holding them in Bunsen flame till they become red hot.This is a simple method for effective sterilization of such
articles, but is limited to those articles that can be heated to redness in flame.
Flaming: This is a method of passing the article over a Bunsen flame, but not heating it to redness.Articles such as
scalpels, mouth of test tubes,flasks, glass slides and cover slips are passed through the flame a few times. Even
though most vegetative cells are killed, there is no guarantee that spores too would die on such short exposure.
Incineration: This is a method of destroying contaminated material by burning them in incinerator. Articles such as
soiled dressings; animal carcasses,pathologicalmaterial and bedding etc should be subjected to incineration. This
technique results in the loss of the article, hence is suitable only for those articles that have to be disposed.
Hot air oven: This method was introduced by Louis Pasteur. Articles to be sterilized are exposed to high
temperature (160oC) for duration of one hour in an electrically heated oven. Different temperature-time relations for
holding time are 60 minutes at 160oC, 40 minutes at 170oC and 20 minutes at 180oC.Increasing temperature by 10
degrees shortens the sterilizing time by 50 percent.
Metallic instruments (like forceps, scalpels, scissors),glasswares (such as petri-dishes, pipettes,flasks, all-glass
syringes), swabs,oils, grease, petroleum jelly and some pharmaceutical products.
At temperature below 100oC:
Pasteurization: This process was originally employed by Louis Pasteur. Currently this procedure is employed in
food and dairy industry.
There are two methods of pasteurization,
The holder method (heated at 63oC for 30 minutes) and flash method (heated at 72oC for 15 seconds)followed by
quickly cooling to 13oC.Other pasteurization methods include Ultra-High Temperature (UHT), 140oC for 15 sec
and 149oC for 0.5sec. This method is suitable to destroy most milk borne pathogens like Salmonella, Mycobacteria,
Streptococci, Staphylococci and Brucella, however Coxiella may survive pasteurization.
Efficacy is tested by phosphatase test and methylene blue test.
Vaccine bath: The contaminating bacteria in a vaccine preparation can be inactivated by heating in a water bath at
60oC for one hour. Only vegetative bacteria are killed and spores survive.
2. Serum bath: The contaminating bacteria in a serum preparation can be inactivated by heating in a water bath at
56oC for one hour on several successive days.Proteins in the serum will coagulate at higher temperature. Only
vegetative bacteria are killed and spores survive.
Inspissation: This is a technique to solidify as well as disinfect egg and serum containing media. The medium
containing serum or egg are placed in the slopes of an inspissatorand heated at 80-85oC for 30minutes on three
successive days.
At temperature 100oC:
Boiling: Boiling water (100oC) kills most vegetative bacteria and viruses immediately. Certain bacterial toxins such
as Staphylococcal enterotoxin are also heat resistant and Some bacterial spores can survive; hence this is not a
substitute forsterilization.
At temperature above 100oC:
Autoclave: Sterilization can be effectively achieved at a temperature above 100oC using an autoclave.Water boils at
100oC at atmospheric pressure,but if pressure is raised, the temperature at which the water boils also increases.In
an autoclave the water is boiled in a closed chamber. As the pressure rises, the boiling point of water also raises. At
a pressure of 15 lbs inside the autoclave, the temperature is said to be 121oC. Exposure of articles to this temperature
for 15 minutes sterilizes them. To destroy the infective agents associated with spongiform encephalopathies (prions),
higher temperatures or longer times are used; 135oC or 121oC for at least one hourare recommended.
Advantages of steam: It has more penetrative power than dry air, it moistens the spores (moisture is essentialfor
coagulation of proteins), condensation ofsteam on cooler surface releases latent heat, condensation ofsteam draws
in fresh steam.
Radiation: Two types of radiation are used, ionizing and non-ionizing. Non-ionizing rays are low energy rays with
poor penetrative power while ionizing rays are high-energy rays with good penetrative power. Since radiation does
not generate heat, it is termed "cold sterilization".
Filtration:
Filtration does not kill microbes, it separates themout. Membrane filters with pore sizes between 0.2-0.45 μm are
commonly used to remove particles from solutions that can't be autoclaved.It is used to remove microbes from heat
labile liquids such as serum, antibiotic solutions,sugarsolutions,urea solution etc.
Different types of filters are:
Earthenware filters: These filters are made up of diatomaceous earth or porcelain. They are usually baked into the
shape of candle.
Asbestos filters: These filters are made from chrysotile type of asbestos,chemically composed of magnesium
silicate.
Sintered glass filters: These are made from finely ground glass that are fused sufficiently to make small particles
adhere to each other.
Membrane filters: These filters are made from a variety of polymeric materials such as cellulose nitrate, cellulose
diacetate, polycarbonate and polyester.
Disinfectants:
A 70% aqueous solution is more effective at killing microbes than absolute alcohols. 70% ethyl alcohol (spirit) is
used as antiseptic on skin. Isopropyl alcohol is preferred to ethanol.
Aldehydes:
3. Mode of action: Acts through alkylation of amino-, carboxyl- or hydroxyl group, and probably damages nucleic
acids. It kills all microorganisms, including spores.
Examples: Formaldehyde, Gluteraldehyde
40% Formaldehyde (formalin) is used for surface disinfection and fumigation of rooms, chambers, operation
theatres, biological safety cabinets, wards, sick rooms etc. Fumigation is achieved by boiling formalin, heating
paraformaldehyde or treating formalin with potassiumpermanganate. It also sterilizes bedding, furniture and books.
10% formalin with 0.5% tetraborate sterilizes clean metal instruments.
2% gluteraldehyde (CIDEX) is used to sterilizen thermometers, cystoscopes, bronchoscopes, centrifuges,
anasethetic equipments etc. An exposure of at least 3 hours at alkaline pH is required for action by gluteraldehyde.
2% formaldehyde at 40oC for 20 minutes is used to disinfect wool and 0.25% at 60oC for six hours to disinfect
animal hair and bristles.
Phenol They act as disinfectants at high concentration and as antiseptics at low concentrations.They are
bactericidal, fungicidal, mycobactericidal but are inactive against spores and most viruses.
Hydrogen Peroxide: It is used at 6% concentration to decontaminate the instruments, equipments such as
ventilators. 3% Hydrogen Peroxide Solution is used for skin disinfection and deodorising wounds and ulcers. Strong
solutions are sporicidal.
Ethylene Oxide (EO): It requires presence of humidity. It has good penetration and is well absorbed by porous
material. It is used to sterilize heat labile articles such as bedding,textiles, rubber, plastics, syringes,disposable
petri dishes,complex apparatus like heart-lung machine, respiratoryand dental equipments.Efficiency testing is done
using Bacillus subtilis var niger.
Halogens (Chroline and iodine): They are oxidizing agents and cause damage by oxidation of essentialsulfydryl
groups of enzymes.
Chlorine reacts with water to form hypochlorous acid, which is microbicidal but cyst of E. histolytica, HAV,HEV,
Polio virus and cyst of Giardia are not susceptible.Chlorine gas is used to bleach water (chlorine 33%)
Tincture of iodine (2% iodine in 70% alcohol) is an antiseptic.Iodine can be combined with neutral carrier
polymers such as polyvinylpyrrolidone to prepare iodophores such as povidone-iodine (Betadine). 10% Povidone
Iodine is used undiluted in pre and postoperative skin disinfection.
Note
Prions are resistance to almost all disinfectant except high doses ofSodium Hypochlorite and Gram Positive
bacteria’s are susceptible to almost all disinfectants.
Order of resistance to disinfectant
Prions > Hydrophilic Virus > Fungi > Acid-Fast organisum > Gram Negative bacteria > Lipophilic Virus > Gram
Positive Bacteria.
Sodium Hypochlorite is best disinfectant and Betadine is best disinfectant for skin.
Testing of Disinfectants
Different methods are:
1. Koch’s method Spores of Bacillus anthracis were dried on silk thread
2. Rideal Walker Method estimation of phenol coefficient
3. Chick Martin test
Unlike in Rideal Walker Method where the test is carried out in water, the disinfectants are made to act in the
presence of yeast suspension (or3% dried human feces). Time for subculture is fixed at 30 minutes and the
organism used to test efficacy is S.typhi as well as S.aureus. The phenol coefficient is lower than that given by
Rideal Walker method.
4. Capacity use dilution test (Kelsey-Sykes test)Inoculum of four different test organisms, namely Staphylococcus
aureus,Escherichia coli, Pseudomonasaeruginosa and Proteus vulgaris are added to the disinfectant in three
successive.
5. In-use test:This test is intended toestimate the number of living organism in a vesselof disinfectant in actual use.
MCQ
4. 1) Lysol is a
a) Sterilent b) Disinfectant c) Antiseptic
d) Antifungal agent
2) The technique used to avoid all microorganisms is accomplished by
a) Sterlization b) Disinfection
c) Surgical sterilization d) Disinfection Sterilization
3) Thermal death time is
a) Time required to kill all cells at a given temperature
b) Temperature that kills all cells in a given time
c) Time and temperature needed to kill all cells
d) All of the above
4) Temperature required for pasteurization is
a) Above 150oC b) Below 100oC c) 110oC d) None
5) Temperature in pasteurization is
a) 62.8oC b) 35.7oC c) 68.2oC d) 60.8oC
6) By pasteurization
a) All the microorganisms can be removed b) Only pathogenic forms can be
removed
c) Only spores d) None
7) Which of the following method of sterilization has no effect on spores?
a) Drying b) Hot air oven c) Autoclave d) None of these
8) Autoclaving is carried at
a) Dry heat b) Atmospheric pressure c) 120oC d) All of
these
9) The condition required for autoclave
a) 121oC temp.and 15 lbs. pressure for 20 min.
b) 120oC temp.and 20 lbs. pressure for 30 min
c) 150oC temp.for 1 hr.
d) 130oC temp for 2 hr.
10) Lyophilization means
a) Sterilization b) Freeze-drying
c) Burning to ashes d) Exposure to formation
11) Temperature used for hot air oven is
a) 100oC for 1 hour b) 120oC for 1 hour
c) 160oC for 1 hour d) 60oC for 1 hour
12) Phenol co-efficient indicates
a) Efficiency of a disinfectant b) Dilution of a disinfectant
c) Purity of a disinfectant d) Quantity of a disinfectant
13) Spores are killed by
a)70% alcohol b) Glutaraldehyde
c) Autoclaving d ) Both b and c
14) Glassware are sterilized by
a) Autoclaving b) Hot air over c) Incineration d) None
of these
5. 15) The lowest temperature that kills all microorganisms in a liquid suspension in 10 minutes is known as the
a)Decimal reduction time
b)Thermal death point.
c)Thermal death temperature.
d)Thermal death time.
16) The time required to kill 90% of the microorganisms in a sample at a specific temperature is the
a) Decimal reduction time.
b)Thermal death point.
c)Thermal death temperature.
d)D value.
17) The time in minutes at a specific temperature needed to kill a population of cells is the
a) Decimal reduction time.
b)Thermal death point.
c)Thermal death temperature.
d)F- value.
18) Which of the following is most effective for sterilizing mattresses and plastic Petri plates?
a)Chlorine
b)Ethylene oxide
c) Glutaraldehyde
d) Autoclave
30 INCORRECT
19) Aldehydes,which are most powerful disinfectants
a) Formaldehyde b) Acetaldehyde c) Glutamal aldehyde d) Both
a and c
20)Sweet and salty foods frequently don't require refrigeration to prevent spoilage because they have
a)Insufficient nutrients.
b)Low pH.
c)High concentration of solutes.
d)Toxic alkaline chemicals.
21) All of the following are used in Kelsey-Sykes test except,
a) Staphylococcus aureus b) Escherichia coli
c) Pseudomonas aeruginosa d) Shigella
22) Which one the following is best test to know the activity of an unknown disinfectant?
a) MIC test b) Rideal Walker test
c) Chick Martin test d) ‘in use’test of Maurer
23) Which of the following is best to sterilized heat labile (heat unstable)solution.
a) Dry heat b) Autoclave c) Membrane
filtration d) Pasteurization
6. 24) Which of the following is bactericidal (lethal to bacteria). a) Membrane filtration b)
Ionizing radiation c) Deep freezing d) All of the above
25) The typical termperature for an autoclave (operating at 15pounds per square inch of pressure)is
a) 121oC b)100oC c)63oC d)73oC
26) Phenol co-efficient indicates
a) Efficiency of a disinfectant
b) Dilution of a disinfectant
c) Purity of a disinfectant
d) Quantity of a disinfectant
1) B 2) A 3) B 4) B 5) A 6) B 7) A
8) C 9) C 10) B 11) C 12) A 13) D 14) B
15) B 16) D 17) D 18) B 19) D 20) C 21) D
22) D 23)C 24)B 25) A 26) A