This document is a standard operating procedure (SOP) for the haematology lab, detailing the process for estimating complete blood count (CBC) using the Erba H560 autoanalyzer. It outlines responsibilities of technical staff, required materials, and step-by-step procedures for sample analysis and machine maintenance. The SOP is authored by Attuluri Vamsi Kumar and approved by Prof. (Dr.) Ranjay Kumar Chaudary, effective from April 1, 2023.

1. STANDARD OPERATING PROCEDURE (SOP)
Lab Name: Haematology Lab Lab In-charge: Attuluri Vamsi Kumar
Experiment Name:
SOP No.
1
Effective date
01-04-2023
Pages
1
Autor
Mr. Attuluri Vamsi
Kumar
Authorized by
Prof. (Dr). Ranjay
Kumar Chaudary
Version No.
1
Review Period No. of copies
2
Lab
Haematology
Location
D7
1. Scope & application:
This SOP describes the method of estimation of CBC including differential count with the help of autoanalyzer
H560
2. Responsibility:
Technical staff deputed in the haematology lab including students
3. References:
Manual of ERBA H560 autoanalyzer
4. Materials required:
o Sample-EDTA Blood
o Test tubes
o PPE
5. Procedure:
➢ Wear the PPE
➢ Turn on the machine and enter the User ID & password.
➢ Wait until the completion of auto clean procedure.
➢ Ensure that the LED light turned to green (If it is red then identify the error and inform the issue to
lab supervisor)
➢ Select the sample Analysis option on the home screen of H560 autoanalyzer
➢ Take an EDTA vial and label it with patient details including reg number, age, sex, others if requied
➢ Collect venous blood aseptically into the EDTA vial
➢ Remove the cap of the vial and place it near the sample probe of the machine
▪ (Note 1: Ensure that the probe is completely submerged into the blood)
▪ (Note 2: At any instant no air bubble must enter into the probe)
➢ Press the aspirate button either on the touch screen or the button provided near the probe area.
➢ Wait until the probe retract inside the machine.
➢ Remove the vial, place the cap and keep it in the test tube stand.
➢ Wait until the completion of analysis operation by the machine.
➢ Take the print of the results displayed in the screen.
6. Maintenance & cleaning: Shutting down the instrument
➢ Take the ERBA Elite H clean solution from the refrigerator
➢ Select the ERBA logo on the top let corner of the screen
➢ Press the power off button shown at the bottom of the screen
➢ Open the Elite H clean bottle cap and place near the probe ensuring complete submerge of the probe
into the solution.
➢ Click aspirate
➢ Waiting until the finishing of close operation by the machine.
➢ Switch of the instrument.

2. H560 User interface