This document summarizes two case studies presented by Merck Serono on using high-throughput cell culture methods to speed up media development. In the first case study, 376 different media blends were tested in parallel fed-batch cultures to identify a new high-performance medium. Data analysis using Excel, design of experiments, and multivariate analysis identified key formulations and components. One new medium showed a 30-60% improvement in titer for three cell lines. The second case study showed that quality attributes of products from 400 micro-scale fed-batch cultures could be analyzed to optimize critical quality attributes. A design of experiments approach tested multiple factors to match quality targets. This enabled confirmation and scale-up of an improved