Signal transduction is the process by which a chemical or physical signal is transmitted through a cell as a series of molecular events, most commonly protein phosphorylation catalyzed by protein kinases, which ultimately results in a cellular response. Proteins responsible for detecting stimuli are generally termed receptors, although in some cases the term sensor is used.The changes elicited by ligand binding (or signal sensing) in a receptor give rise to a biochemical cascade, which is a chain of biochemical events as a signaling pathway.When signaling pathways interact with one another they form networks, which allow cellular responses to be coordinated, often by combinatorial signaling events. At the molecular level, such responses include changes in the transcription or translation of genes, and post-translational and conformational changes in proteins, as well as changes in their location. These molecular events are the basic mechanisms controlling cell growth, proliferation, metabolism and many other processes.In multicellular organisms, signal transduction pathways have evolved to regulate cell communication in a wide variety of ways.
GENERAL IDEA OF SIGNAL TRANSDUCTION
DEFINATION
WHAT DOES THE TERM SIGNAL TRANSDUCTION MEANS
HISTORY
BASIC ELEMENTS IN SIGNAL TRANSDUCTION
TYPES OF SIGNAL TRANSDUCTION
SIGNALLING MOLECULE
RECEPTOR MOLECULE
MODES OF CELL CELL SIGNALING
SECOND MESSENGER
SIGNAL TRANSDUCTION PATHWAY
SOME SIGNALING PATHWAYS
SIGNIFICANCE
CONCLUSION
REFERENCE
GENERAL IDEA OF SIGNAL TRANSDUCTION
DEFINATION
WHAT DOES THE TERM SIGNAL TRANSDUCTION MEANS
HISTORY
BASIC ELEMENTS IN SIGNAL TRANSDUCTION
TYPES OF SIGNAL TRANSDUCTION
SIGNALLING MOLECULE
RECEPTOR MOLECULE
MODES OF CELL CELL SIGNALING
SECOND MESSENGER
SIGNAL TRANSDUCTION PATHWAY
SOME SIGNALING PATHWAYS
SIGNIFICANCE
CONCLUSION
REFERENCE
Cells of multicellular organisms detect and respond to countless internal and extracellular signals that control their growth, division, and differentiation during development, as well as their behavior in adult tissues.
At the heart of all these communication systems are regulatory proteins that produce chemical signals, which are sent from one place to another in the body or within a cell, usually being processed along the way and integrated with other signals to provide clear and effective communication.
Study of cell signaling has traditionally focused on the mechanisms by which eukaryotic cells communicate with each other using extracellular signal molecules such as hormones and growth factors.
Many bacteria, respond to chemical signals that are secreted by their neighbors and accumulate at higher population density. This process, called quorum sensing, allows bacteria to coordinate their behavior, including their motility, antibiotic production, spore formation, and sexual conjugation.
Communication between cells in multicellular organisms is mediated mainly by extracellular signal molecules.
Most cells in multicellular organisms both emit and receive signals. Reception of the signals depends on receptor proteins, usually (but not always) at the cell surface, which bind the signal molecule. The binding activates the receptor, which in turn activates one or more intracellular signaling pathways or systems.
These systems depend on intracellular signaling proteins, which process the signal inside the receiving cell and distribute it to the appropriate intracellular targets.
The targets that lie at the end of signaling pathways are generally called effector proteins, which are altered in some way by the incoming signal and implement the appropriate change in cell behavior.
Depending on the signal and the type and state of the receiving cell, these effectors can be transcription regulators, ion channels, components of a metabolic pathway, or parts of the cytoskeleton.
This Presentation provides an outline knowledge about Cellular Communication, Steps involved, Its Types, Signal Transduction, Secondary Messenger , Receptors with some Interesting Facts and Current Trends. An assignment for the subject, Cellular and Molecular Pharmacology, 1st year M.Pharm, 1st semester.
ntroduction
2. Definition
3. Steps Of Signal Transduction
A) Reception
B) Transduction
C) Induction
4. Important component used in Signal Transduction
A) Calcium ion as second messenger
B) Protein Kinase
Types of Signal Transduction
A) Extra cellular Signal Transduction
B) Intra cellular Signal Transduction
C) Inter cellular Signal Transduction
6. Mechanism of Signal Transduction
A) GPCR pathway
B) RTK pathway
7. Example of Signal Transduction
A) In plants
B) In animals
8. Conclusion
9. Reference…
Cells of multicellular organisms detect and respond to countless internal and extracellular signals that control their growth, division, and differentiation during development, as well as their behavior in adult tissues.
At the heart of all these communication systems are regulatory proteins that produce chemical signals, which are sent from one place to another in the body or within a cell, usually being processed along the way and integrated with other signals to provide clear and effective communication.
Study of cell signaling has traditionally focused on the mechanisms by which eukaryotic cells communicate with each other using extracellular signal molecules such as hormones and growth factors.
Many bacteria, respond to chemical signals that are secreted by their neighbors and accumulate at higher population density. This process, called quorum sensing, allows bacteria to coordinate their behavior, including their motility, antibiotic production, spore formation, and sexual conjugation.
Communication between cells in multicellular organisms is mediated mainly by extracellular signal molecules.
Most cells in multicellular organisms both emit and receive signals. Reception of the signals depends on receptor proteins, usually (but not always) at the cell surface, which bind the signal molecule. The binding activates the receptor, which in turn activates one or more intracellular signaling pathways or systems.
These systems depend on intracellular signaling proteins, which process the signal inside the receiving cell and distribute it to the appropriate intracellular targets.
The targets that lie at the end of signaling pathways are generally called effector proteins, which are altered in some way by the incoming signal and implement the appropriate change in cell behavior.
Depending on the signal and the type and state of the receiving cell, these effectors can be transcription regulators, ion channels, components of a metabolic pathway, or parts of the cytoskeleton.
This Presentation provides an outline knowledge about Cellular Communication, Steps involved, Its Types, Signal Transduction, Secondary Messenger , Receptors with some Interesting Facts and Current Trends. An assignment for the subject, Cellular and Molecular Pharmacology, 1st year M.Pharm, 1st semester.
ntroduction
2. Definition
3. Steps Of Signal Transduction
A) Reception
B) Transduction
C) Induction
4. Important component used in Signal Transduction
A) Calcium ion as second messenger
B) Protein Kinase
Types of Signal Transduction
A) Extra cellular Signal Transduction
B) Intra cellular Signal Transduction
C) Inter cellular Signal Transduction
6. Mechanism of Signal Transduction
A) GPCR pathway
B) RTK pathway
7. Example of Signal Transduction
A) In plants
B) In animals
8. Conclusion
9. Reference…
Cells respond to nutrient deprivation a variety of ways. In addition to global down regulation of cap-dependent protein
synthesis mediated by the GCN2 and mTO RC1 signaling pathways, a catabolic process autophagy is upregulated to
provide internal building blocks and energy needed to sustain viability. It has recently been shown that during nutrient
deprivation tRNAs accumulate in the nucleus, but the functional role of this accumulation remains unknown. This study
investigates whether subcellular localization of tRNAs plays a role in signaling nutritional stress and autophagy. We report
that human fibroblasts that accumulate tRNA in the nucleus due to downregulation of their transportin, Xpo-t, show
reduced mTO RC1 activity and upregulated autophagy. This suggests that sub-cellular localization of tRNAs may regulate
an unicellular response to starvation independently of the cellular nutritional status.
Direct Lineage Reprogramming: Novel Factors involved in Lineage ReprogrammingAhmed Madni
Direct linage reprogramming has got a major focus in biomedical field. The production of specific functional cell type from totally different cell lineage is called lineage reprogramming. In other words, it is induction of functional cell type from another linage without passing through intermediate stage of pluripotent.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
1. Signal Transduction:
Introduction:
Signal transduction is the process by which a chemical or physical signal is transmitted through a cell as a
series of molecular events, most commonly protein phosphorylation catalyzed by protein kinases, which
ultimately results in a cellular response. Proteins responsible for detecting stimuli are generally termed
receptors, although in some cases the term sensor is used.The changes elicited by ligand binding (or
signal sensing) in a receptor give rise to a biochemical cascade, which is a chain of biochemical events as
a signaling pathway.When signaling pathways interact with one another they form networks, which allow
cellular responses to be coordinated, often by combinatorial signaling events. At the molecular level, such
responses include changes in the transcription or translation of genes, and post-translational and
conformational changes in proteins, as well as changes in their location. These molecular events are the
basic mechanisms controlling cell growth, proliferation, metabolism and many other processes.In
multicellular organisms, signal transduction pathways have evolved to regulate cell communication in a
wide variety of ways.
Each component (or node) of a signaling pathway is classified according to the role it
plays with respect to the initial stimulus. Ligands are termed first messengers, while receptors are the
signal transducers, which then activate primary effectors. Such effectors are often linked to second
messengers, which can activate secondary effectors, and so on. Depending on the efficiency of the nodes,
a signal can be amplified (a concept known as signal gain), so that one signaling molecule can generate a
response involving hundreds to millions of molecules. As with other signals, the transduction of
biological signals is characterised by delay, noise, signal feedback and feedforward and interference,
which can range from negligible to pathological. With the advent of computational biology, the analysis
of signaling pathways and networks has become an essential tool to understand cellular functions and
disease, including signaling rewiring mechanisms underlying responses to acquired drug resistance
Pathophysiology Defined by Altered Signal Transduction Pathways: The Role of JAK-STAT and
PI3K Signaling in Leukemic Large Granular Lymphocytes:
2. Signal transduction pathways integrate a variety of micro environmental cues to guide cell function by
regulating gene transcription, cell cycle status, growth, and differentiation. It is well established that
perturbation of these processes plays a key role in hematologic malignancies including lymphomas and
chronic and acute lymphocytic leukemias. Altered intracellular signaling pathways have been proposed to
mediate many biological properties of T cell large granular lymphocytic leukemia (T‑LGL), a disorder
characterized by a clonal proliferation of CD8 T cells resulting in immune‑mediated cytopenias,most
commonly neutropenia. Since T‑LGL offers a unique opportunity to study signal transduction in the
pathologic clonal cytotoxic T cell (CTL) compared to normal CTL, we have investigated a potential
imbalance in T‑LGL pro‑survival signaling to define the mechanisms underlying the semi‑autonomous
proliferation leading to leukemia. Increased activity of the PI3K‑AKT signaling axis in T‑LGL cells
appears to operate in conjunction with or parallel to increased STAT3 activation in these cells to inhibit
the apoptotic program. Thus, the ability to define pathophysiology at the molecular level opens new
avenues for targeted therapeutics. The well‑orchestrated induction of signal transduction pathways defines
cellular behavior through complex interactions that are only beginning to be worked out in experimental
systems. Despite the daunting task of comprehending all aspects of the entire networked system, focused
approach of interrogating specific signaling pathways,depending on the hypothesis being tested can
provide useful information regarding pathophysiology and yield potential therapeutic targets. The highly
dynamic nature of the immune system, with a multitude of interconnected accessory receptor and
cytokine receptor signaling pathways, is an ideal setting to test this premise.
Nature of Clonal Expansion of in CTL from T‑LGL and their in Normal Counterparts
Within the cell‑mediated arm of the host immune response, cytotoxic CD8 T cells (CTL) have a
well‑defined role in targeted killing of malignant and virus‑infected cells. CTL activation is initiated via T
cell receptor signaling, and augmented through a multitude of accessory receptor‑ligand signaling systems
resulting in the production of cytokines and release of cytotoxic factors. In the waning phase of an
immune response, signal transduction pathways are initiated to instruct the vast majority of activated CD8
T cells directed against inciting antigens to undergo apoptosis.Failure of this signaling system due to
genetic lesions has been linked to lymphoproliferative disorders.Thus, it is rational to link malfunction of
apoptotic mechanisms to autoimmune disease. In certain individuals, a T cell clone may emerge that is
largely resistant to the factors driving a return to the homeostatic set point, thus setting the stage for a
neoplastic process. T cell large granular lymphocytic leukemia (T‑LGL) is a suitable model to study
physiologic and pathophysiologic regulation of CTL clonal expansion and persistence.
Transduction in T‑LGL T‑LGL is a clonal lymphoproliferative process of CD8 cytotoxic T cells, often
arising in the context of various autoimmune conditions and resembling a natural CTL response
to viral infection.A key aspect of this disease is the failure of previously activated cytotoxic T cells to
undergo apoptosis, despite appropriate expression of Fas and FasL. However, the T‑LGL cells are
relatively insensitive to anti‑Fas or FasL induced apoptosis.A molecular mechanism for apoptotis
resistance was recently proposed with the finding that STAT3 is constitutively phosphorylated at tyrosine
705 in T‑LGL, resulting in increased DNA binding activity and transcription of the anti‑apoptotic protein
Mcl‑1.14 Since T‑LGL is in large part a disease characterized by failure to undergo homeostatic apoptosis
by a single (or few) CTL clones, we considered additional pro‑survival pathways that may be aberrantly
activated in these cells and analyzed a possible link between insensitivity to Fas‑induced apoptotic
signaling and increased activation of STAT3. The involvement of phosphatidylinositol‑3 kinase (PI3K)
signal transduction pathway in the LGL dysregulation was considered likely because of its ability to
inhibit Fas signaling15 and its role in regulating STAT3 serine phosphorylation in primary T cells,16
believed to be required for optimal transcriptional activation.We found increased PI3K‑AKT pathway
activity in T‑LGL cells compared to T cells from healthy donors, suggesting inappropriate activation of
this pro‑survival signaling pathway. A proposed mechanism for JAK‑STAT and PI3K pathway crosstalk
3. in T cells involves the ERK mitogen activated protein kinase pathway (MAPK).We have previously
shown that optimal phosphorylation of ERK in primary T cells is dependent on the prior activation of the
PI3K‑AKT pathway. Thus increased PI3K‑AKT activation may indeed be decreasing the threshold for
ERK activation in T‑LGL cells and positively influencing JAK‑STAT signaling . However, it is also
possible that these two pathways are acting in parallel to enhance cell survival.A key question that needs
to be addressed is how the PI3K‑AKT pathway is aberrantly activated in the T‑LGL cells. A simplistic
way of approaching this question is to consider the opposing effects of activating factors that induce
pathway activation and inhibitory factors that dampen pathway activation. For instance, LGL patients
show a constitutively upregulated production of IL‑18, RANTES, and MIP‑1b, which are known to
activate the PI3K pathway.Thus, it is possible that increased PI3K‑AKT pathway activation is drivenin
part by increased cytokine receptor signaling.
Since our recent publication in Blood describing Src family kinase (SFK) dependent PI3K‑AKT pathway
activation in T‑LGL,we have identified potential defects in the negative regulation of this signaling
network as well, based on results from gene expression profiling (manuscript submitted). In particular, we
have observed decreased expression of three genes in particular that relate to control of SFK activity
(PAG1) and PI3K‑AKT activation (PTEN and PIK3R1). Thus, it is possible that the normal “brakes”
regulating the pro‑survival signaling network are not functioning optimally . Since signal transduction
pathways operate as cascades, it is conceivable that a small perturbation in the proximal part of the
pathway, due to slightly decreased inhibition, will be amplified in the downstream elements regulating
survival versus apoptosis (such an effect may be further exaggerated in the context of continuous cytokine
receptor activation, or persistent antigenic drive, e.g., during an autoimmune process). The role of
increased SFK activity in T‑LGL, and its requirement for the increased activity of the PI3K‑AKT
pathway, is interesting in light of long‑sought association of T‑LGL with a viral infection.Several viruses
have demonstrated an ability to induce PI3K‑AKT.
activity in an SFK dependent fashion. This effect was associated with the ability of virus molecular
machinery to inhibit host cell apoptosis. While no definitive viral etiology has been linked with T‑LGL,
this area is under active investigation. The possibility that SFK activation plays a role in T‑LGL
pathogenesis is extended by our recent observation that mRNA expression of phosphoprotein associated
with glycolipid‑enriched domains (PAG) is decreased in these cells compared to healthy individuals
(unpublished observations). Consequently one could hypothesize that the reduced levels of PAG will
diminish the membrane recruitment of Csk, a kinase that associates with PAG and phosphorylates the
inhibitory C‑terminal tyrosine in SFKs24 . Thus, the SFK activity in glycolipidenriched domains, also
referred to as lipid rafts, of T‑LGL cells would be missing an important inhibitory signal and thus have a
lower threshold for activation. Elucidating the relationship of PAG, Csk, and the different phosphorylated
forms of SFK (positive regulatory site in the kinase loop domain and negative regulatory Csk‑targeted site
near the C‑terminus) in lipid rafts may help to explain the mechanism for the increased SFK activation we
see in T‑LGL. In addition, we are investigating the spatial context in which lipid raft associated SFK
interact with and positively influence PI3K and/or AKT kinase activity, either directly or via inhibition of
PTEN. It is likely that a greater understanding of this networking interaction (SFK pathways interacting
with the PI3K pathway) will shed light on the mechanisms by which the anti‑apoptotic PI3K pathway
may be regulated in normal and disease states. The decreased PTEN expression in T‑LGL cells, noted
above, is an interesting finding in that this is a known mechanism for neoplastic cells to thwart the normal
balance of PI3K signaling.In conjunction with any negative regulation by increased SFK activity,25
decreased PTEN could markedly hinder the ability to keep PI3K dependent pathways in check. A less
obvious mechanism for a relative increase in PI3K activity might be decreased expression of p85a
(PIK3R1), a regulatory subunit of class IA PI3K enzymes. While the class I PI3K enzymes function as
heterodimers of regulatory and catalytic subunits, it has been shown that decreased expression of the
regulatory subunit can actually increase the activation of PI3K.For example, in wild‑type fibroblasts, the
4. p85 subunit was more abundant than p110, leading to competition between the p85 monomer and the
p85‑p110 dimer for receptor binding sites and diminished PI3K signaling. When p85 was reduced by
genetic means, PI3K signaling was enhanced, suggesting that a new balance between the lone regulatory
subunit and the p85/p110 dimer had been reached.Consequently, aberrant activation of PI3K‑AKT
pathway in T‑LGL cells in the context of decreased PIK3R1 expression, may suggest yet another
defective negative regulatory pathway, allowing pro‑survival signaling to continue with less inhibitory
input. We are currently studying the potential crosstalk between the PI3K‑AKT pathway and the
JAK‑STAT pathway in T‑LGL. While the published role of ERK as an intermediary in this regard is
appealing, the role of STAT3 (S727) phosphorylation in CTL activation needs to be better defined.
Notably, in other tissues, (e.g., mammary epithelial cells), the relationship between STAT3 activation and
PI3K‑AKT is quite different: mammary gland involution is dependent on STAT3 mediated apoptosis, due
in part to PI3K‑AKT inhibition.
This is in stark contrast to T‑LGL cells, in which STAT3 drives transcription of
the anti‑apoptotic protein Mcl‑1, highlighting how signaling pathways interact to direct effector function
in a cell-specific manner.14 Inhibition of the JAK‑STAT pathway via the tyrphostin AG490 resulted in
apoptosis of the T‑LGL cells, illustrating the importance of the cellular context in which signal
transduction pathways are networked together. Thus, evolutionary processes have selected for modular
signaling pathways that can be assembled in unique spatial and temporal configurations to achieve cell
and tissue‑type dependent responses. Aberrant survival signaling in T‑LGL cells serve an important
reminder that pathologic activation of signal transduction pathways is not necessarily due to
overexpression of signaling components, such as that classically seen in Her2/Neu amplification in breast
cancer,or to genetic lesions creating increased intrinsic enzymatic activity, such as the recently described
JAK2 V617F mutation in myeloproliferative disorders.
We believe that it is important to consider a loss of the inhibitory factors that are
required to maintain a dynamic homeostasis. Decreased expression of adaptor molecules that possess no
intrinsic enzymatic activity themselves, such as PAG, may be easily overlooked, but can play an
important role in regulating the overall signaling network. Decreased expression of the PTEN protein, or
a mutation resulting in decreased phosphatase activity is an obvious clue that the PI3K‑AKT pathway
may lack an important regulatory input. However, downmodulation of the p85a subunit of the PI3K
heterodimeric enzyme may result in a decreased ratio of free p85aa to p85a in complex with the catalytic
subunit. This subtle loss of PI3K regulation at the level of initial recruitment to activated receptors may
have profound functional consequences.
Conclusion:
Signal transduction, and ultimately signaling networks, becomes more sophisticated, so to must our
appreciation for the myriad controls that operate on many levels. The fact that signaling pathways may be
differentially constructed and regulated in various tissue types may at first glance seem to defy our ability
to understand these pathways in any meaningful detail. However, we believe that this provides an
excellent opportunity to observe how different modular signaling components can be used to integrate
common signals into unique cellular and tissue responses,and ultimately delineate novel targets for
emerging therapies.
5. Signal transduction by the epidermal growth factor receptor after functional desensitization of the
receptor serine protein kinase activity:
Introduction:
Previous work identified a protein kinase activity that phosphorylates the epidermal growth factor (EGF)
receptor at Thr"'. An assay for this protein kinase activity present in homogenates prepared from A431
human epidermoid carcinoma cells was developed using a synthetic peptide substrate corresponding to
residues 663-681 of the EGF receptor (peptide T669). Here we report that a greater initial rate
of T669 phosphorylation was observed in experiments using homogenates prepared from EGF- or
phorbol ester-treated cells compared with control cells. EGF and 4p-phorbol 12- myristate 13-acetate
(PMA) caused a 6-fold and a 2-fold increase in protein kinase activity, respectively. A kinetic analysis of
T669 phosphorylation demonstrated that the increase in protein kinase activity observed was accounted
for by an increase in Vmax. To examine the interaction between protein kinase C and signal transduction
by the EGF receptor, the effect of pretreatment of cells with PMA on the subsequent response to EGF was
investigated. Treatment of cells with PMA caused >90% inhibition of the EGF-stimulated tyrosine
phosphorylation of the EGF receptor and abolished the EGFstimulated formation of soluble inositol
6. phosphates. In contrast, PMA was not observed to inhibit the stimulation of T669 protein kinase activity
caused by EGF. Thus, the apparent functional desensitization of the EGF receptor caused by PMA
does not inhibit signal transduction mediated by the T669 protein kinase. Our results demonstrate that
EGF receptor transmodulation alters the pattern of signal-transduction pathways that are utilized by the
EGF receptor. Transmembrane signaling by the epidermal growth factor (EGF) receptor is acutely
regulated by protein kinase C. Activation of protein kinase C by treatment of cells with phorbol ester
tumor promoters causes an inhibition of the high-affinity binding of EGF to cell surface receptors and
causes an inhibition of the receptor tyrosine protein kinase activity . This process of acute regulation of
EGF receptor function has been termed transmodulation .
The molecular mechanism that accounts for transmodulation and the role of
transmodulation in signal transduction by the EGF receptor are not understood. However, signaling by
the transmodulated EGF receptor has been studied in detail. It has been demonstrated that EGF-stimulated
phosphatidylinositol turnover and the generation of soluble inositol phosphates are inhibited by
incubation of cells with phorbol ester . Phorbol ester also inhibits the increase in cytosolic Ca2+ caused
by EGF without affecting basal cytosolic Ca2+ levels . Furthermore, the EGF-stimulated Na+/H+
exchange across the plasma membrane is inhibited by phorbol ester .
Together, these data indicate that protein kinase C causes an acute desensitization of the
function of the EGF receptor. Recently we described a protein kinase activity in cell homogenates that
phosphorylates the EGF receptor at threonine- 669 . An assay for this protein kinase activity was
developed using a synthetic peptide substrate corresponding to residues 663-681 of the EGF receptor
(peptide T669). Treatment of cells with EGF caused a marked increase in the level of T669 protein kinase
activity detected compared with control cells . The stimulation of T669 protein kinase activity caused by
EGF indicates that this enzyme may have an important function in signal transduction. The purpose of the
experiments described in this report was to examine the role of protein kinase C in the regulation of T669
protein kinase activity by EGF.
Conclusion:
Transmodulation ofthe EGF receptor has been reported to cause the desensitization of EGF receptor
function . The observation reported here that the transmodulated EGF receptor is capable of signal
transduction (mediated by the T669 protein kinase) indicates that transmodulation does not result in the
complete desensitization of EGF receptor function. Instead, the results of this study demonstrate that EGF
receptor transmodulation changes the pattern of signal-transduction pathways that are utilized by the EGF
receptor. This conclusion may be physiologically significant because EGF receptor transmodulation
caused by platelet- derived growth factor results in the synergistic stimulation of the growth of cells
cultured with EGF.
7.
8. G protein–coupled receptors:
1.G protein–coupled receptors (GPCRs) are a family of integral transmembrane proteins that possess
seven transmembrane domains and are linked to a heterotrimeric G protein. With nearly 800 members,
this is the largest family of membrane proteins and receptors in mammals. Counting all animal species,
they add up to over 5000.
2.Mammalian GPCRs are classified into 5 major families: rhodopsin-like, secretin-like, metabotropic
glutamate, adhesion and frizzled/smoothened, with a few GPCR groups being difficult to classify due to
low sequence similarity, e.g. vomeronasal receptors.Other classes exist in eukaryotes, such as the
Dictyostelium cyclic AMP receptors and fungal mating pheromone receptors.Signal transduction by a
GPCR begins with an inactive G protein coupled to the receptor; the G protein exists as a heterotrimer
consisting of Gα, Gβ, and Gγ subunits.
3.Once the GPCR recognizes a ligand, the conformation of the receptor changes to activate the G protein,
causing Gα to bind a molecule of GTP and dissociate from the other two G-protein subunits. The
dissociation exposes sites on the subunits that can interact with other molecules.
4.The activated G protein subunits detach from the receptor and initiate signaling from many downstream
effector proteins such as phospholipases and ion channels, the latter permitting the release of second
messenger molecules.
9. 5.The total strength of signal amplification by a GPCR is determined by the lifetimes of the ligand-
receptor complex and receptor-effector protein complex and the deactivation time of the activated
receptor and effectors through intrinsic enzymatic activity; e.g. via protein kinase phosphorylation or b-
arrestin-dependent internalization.A study was conducted where a point mutation was inserted into the
gene encoding the chemokine receptor CXCR2; mutated cells underwent a malignant transformation due
to the expression of CXCR2 in an active conformation despite the absence of chemokine-binding. This
meant that chemokine receptors can contribute to cancer development.
Tyrosine, Ser/Thr and Histidine-specific protein kinases:
1.Receptor tyrosine kinases (RTKs) are transmembrane proteins with an intracellular kinase domain and
an extracellular domain that binds ligands; examples include growth factor receptors such as the insulin
receptor.To perform signal transduction, RTKs need to form dimers in the plasma membrane;the dimer is
stabilized by ligands binding to the receptor.
2.The interaction between the cytoplasmic domains stimulates the autophosphorylation of tyrosine
residues within the intracellular kinase domains of the RTKs, causing conformational changes.
Subsequent to this, the receptors' kinase domains are activated, initiating phosphorylation signaling
cascades of downstream cytoplasmic molecules that facilitate various cellular processes such as cell
differentiation and metabolism.
3.Many Ser/Thr and dual-specificity protein kinases are important for signal transduction, either acting
downstream of [receptor tyrosine kinases], or as membrane-embedded or cell-soluble versions in their
own right. The process of signal transduction involves around 560 known protein kinases and
pseudokinases, encoded by the human kinome
4.As is the case with GPCRs, proteins that bind GTP play a major role in signal transduction from the
activated RTK into the cell. In this case, the G proteins are members of the Ras, Rho, and Raf families,
referred to collectively as small G proteins. They act as molecular switches usually tethered to membranes
by isoprenyl groups linked to their carboxyl ends.
5.Upon activation, they assign proteins to specific membrane subdomains where they participate in
signaling. Activated RTKs in turn activate small G proteins that activate guanine nucleotide exchange
factors such as SOS1. Once activated, these exchange factors can activate more small G proteins, thus
amplifying the receptor's initial signal.
6. The mutation of certain RTK genes, as with that of GPCRs, can result in the expression of receptors
that exist in a constitutively activated state; such mutated genes may act as oncogenes.Histidine-specific
protein kinases are structurally distinct from other protein kinases and are found in prokaryotes, fungi,
and plants as part of a two-component signal transduction mechanism: a phosphate group from ATP is
first added to a histidine residue within the kinase, then transferred to an aspartate residue on a receiver
domain on a different protein or the kinase itself, thus activating the aspartate residue.