This presentation is made for S.Y.Bsc. Students.
The presentation includes Wastewater microbiology. The presentation includes information about sources as well as methods of wastewater treatment.
This ppt contains all types of Microbial Bioremediation methods . Everyone can understand clearly . Explaining with neat pictures and animation . Useful for presentation about Microbes in bioremediation . At last it contains a small animated video which helps to get clear view .
•Introduction of bioremediation: Bioremediation refers to the process of using microorganisms to remove the environmental pollutants i.e. toxic wastes found in soil, water, air etc.
•In situ bioremediation:
It involves a direct approach for the microbial
degradation of xenobiotics at the sites of pollution
(soil, ground water).
•Types of in situ bioremediation:
Natural attenuation.
Engineered in situ bioremediation.
- Bioventing, biosparging, bioslurping,
phytoremediation.
•Ex situ bioremediation:
Waste or toxic pollutants can be collected from the polluted sites and bioremediation can be carried out at a designated place or site.
• Types of ex situ bioremediation
Land farming, windrow, biopiles, bioreactors.
•Microorganisms use in bioremediation:
A number of naturally occurring marine microbes
such as Pseudomonas sp. is capable of degrading oil and other hydrocarbons.
•Factors affecting bioremediation:
Nutrient availability, moisture content, pH, temperature, contaminant availability.
•References:
Satyanarayana U. Biotechnology. BOOKS AND ALLIED (P) Ltd.
Sharma P.D. Environmental Microbiology. RASTOGI PUBLICATIONS.
Gupta P.K. Biotechnology and Genomics. RASTOGI PUBLICATIONS.
Dubey R.C. A Textbook of Biotechnology. S Chand And Company Ltd.
Dubey R.C. A Textbook of Microbiology. S Chand And Company Ltd.
Willey/Sherwood/Woolverton. Prescott’s Microbiology. McGRAW-HILL INTERNATIONAL EDITION.
www.sciencedirect.com/bioremediation.
This presentation is made for S.Y.Bsc. Students.
The presentation includes Wastewater microbiology. The presentation includes information about sources as well as methods of wastewater treatment.
This ppt contains all types of Microbial Bioremediation methods . Everyone can understand clearly . Explaining with neat pictures and animation . Useful for presentation about Microbes in bioremediation . At last it contains a small animated video which helps to get clear view .
•Introduction of bioremediation: Bioremediation refers to the process of using microorganisms to remove the environmental pollutants i.e. toxic wastes found in soil, water, air etc.
•In situ bioremediation:
It involves a direct approach for the microbial
degradation of xenobiotics at the sites of pollution
(soil, ground water).
•Types of in situ bioremediation:
Natural attenuation.
Engineered in situ bioremediation.
- Bioventing, biosparging, bioslurping,
phytoremediation.
•Ex situ bioremediation:
Waste or toxic pollutants can be collected from the polluted sites and bioremediation can be carried out at a designated place or site.
• Types of ex situ bioremediation
Land farming, windrow, biopiles, bioreactors.
•Microorganisms use in bioremediation:
A number of naturally occurring marine microbes
such as Pseudomonas sp. is capable of degrading oil and other hydrocarbons.
•Factors affecting bioremediation:
Nutrient availability, moisture content, pH, temperature, contaminant availability.
•References:
Satyanarayana U. Biotechnology. BOOKS AND ALLIED (P) Ltd.
Sharma P.D. Environmental Microbiology. RASTOGI PUBLICATIONS.
Gupta P.K. Biotechnology and Genomics. RASTOGI PUBLICATIONS.
Dubey R.C. A Textbook of Biotechnology. S Chand And Company Ltd.
Dubey R.C. A Textbook of Microbiology. S Chand And Company Ltd.
Willey/Sherwood/Woolverton. Prescott’s Microbiology. McGRAW-HILL INTERNATIONAL EDITION.
www.sciencedirect.com/bioremediation.
Bioremediation of heavy metals pollution by Udaykumar Pankajkumar BhanushaliUdayBhanushali111
Mechanisms and techniques used for Bioremediation which includes phytoremediation, Bacterial & fungal bioremediation. Examples of heavy metal pollution
Basic Knowledge about industrial microorganism. why industry choose microorganism rather than chemical. isolation technique of microorganism. source of microorganisms. Process of using microorganism. Disadvantages of using microorganisms in industry. Process of genetic modification of microorganisms. Storage process of microorganism. preservation methods of microorganism. Reculture methods of microorganism.
microbial degradation in waste managementpgayatrinaidu
What is Waste? Types of Waste
What is untreated waste?
Why do we need to treat waste?
Effects of untreated waste on environment
Methods of waste treatment
What is Microbial Degradation?
Types of microbial degradation
Role of microbial degradation in waste management
Bioremediation of heavy metals pollution by Udaykumar Pankajkumar BhanushaliUdayBhanushali111
Mechanisms and techniques used for Bioremediation which includes phytoremediation, Bacterial & fungal bioremediation. Examples of heavy metal pollution
Basic Knowledge about industrial microorganism. why industry choose microorganism rather than chemical. isolation technique of microorganism. source of microorganisms. Process of using microorganism. Disadvantages of using microorganisms in industry. Process of genetic modification of microorganisms. Storage process of microorganism. preservation methods of microorganism. Reculture methods of microorganism.
microbial degradation in waste managementpgayatrinaidu
What is Waste? Types of Waste
What is untreated waste?
Why do we need to treat waste?
Effects of untreated waste on environment
Methods of waste treatment
What is Microbial Degradation?
Types of microbial degradation
Role of microbial degradation in waste management
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
4. DEFINATIONS :
Technology
•It is derived from
living things and their
natural process.
Biotechnology
•A Technology that
uses biological
systems or living
organisims to create
or modify a product
for a particular
purpose.
•It is used to often in
genetic engineering
technology of the
21st
century
5. GENERAL CATEGORIES OF
BIOTECHNOLOGY
•MEDICAL
•vaccine ,diagnostic ,pharmaceuticals
•INDUSTRIAL
•Enzymes & M.O for processing products
•AGRICULTURAL
•Enhance crops ,feeds & fertilizers
•Enviornmental biotechnology – M.o for bioremediation.
6. WHAT IS POLLUTION ?
Pollution
•Pollution is the introduction of contaminants
into the natural environment that cause
adverse change. Pollution can take the form
of any substance or energy. Pollutants, the
components of pollution, can be either
foreign substances/energies or naturally
occurring contaminants(Hg,Ar,Rn)
7. INTRODUCTION
Industrial biotechnology it is often referred to as White technology is a
collection of scientific techniques and technologies used to improve both efficiency and
enviormental foot print of modern industrial production.
Microbial technology constitutes the core of industrial biotechnology.
Microbioal technology refers to the use of microbes to obtain a product or service of
economic value .it is called as fermentation.
8. ACTIVITES IN INDUSTRIAL MICROBIOLOGY
•Isolation of m.o from nature
•Their screening for product formation
•Improvement of product yields.
•Maintenance of cultures
•Mass cultures using bioreactors
•Recovery of products or services
9. APPLICATION
•Metabolic production
•Anaerobic digestion ( for methane production)
•Waste treatment ( both organic and industrial)
•Production of biocontrol agents
•Fermentation of food products
•Bio based & fuel energy
10. METABOLIC PRODUCTION
•M.o produce a number of metabolites during their growth using cheap substrates.
•Acetone-Butanol
•Alcohol
•Antibiotics
•Vitamins
•Enzymes
•Organic acids
11. BIOMASS PRODUCTION
•M.O employee to enhance the availability of nutrients viz nitrogen and
phosphorous to the crops are called biofertilizers.
•BLUE – GREEN ALGAE AND AZOLLA
•AZOTOBACTER & AZOSPIRILLUM
12. ENVIORNMENTAL BIOTECHNOLOGY
• Environmental biotechnology is biotechnology that is applied to and used to study the natural
environment. Environmental biotechnology could also imply that one try to harness biological
process for commercial uses and exploitation. The International Society for Environmental
Biotechnology defines environmental biotechnology as "the development, use and regulation of
biological systems for remediation of contaminated environments (land, air, water), and
for environment-friendly processes (green manufacturing technologies and sustainable
development)".
• Environmental biotechnology can simply be described as "the optimal use of nature, in the form
of plants, animals, bacteria, fungi and algae, to produce renewable energy, food and nutrients in
a synergistic integrated cycle of profit making processes where the waste of each process becomes
the feedstock for another process".
13. SIGNIFICANCE TOWARDS INDUSTRIAL
BIOTECHNOLOGY
•Consider the effluents of starch plant which has mixed up with a local water
body like a lake or pond. We find huge deposits of starch which are not so
easily taken up for degradation by microorganisms except for a few
exemptions. Microorganisms from the polluted site are scan for genomic
changes that allow them to degrade/utilize the starch better than other
microbes of the same genus. The modified genes are then identified. The
resultant genes are cloned into industrially significant microorganisms and
are used for economically processes like in pharmaceutical industry,
fermentations... etc..
14. •Microbes isolated from pesticide-contaminated soils may capable of utilizing
the pesticides as energy source and hence when mixed along with
bio-fertilizers, could serve as an insurance against increased pesticide-toxicity
levels in agricultural platform.
•Similar situations can be encountered in the case of marine oil spills which
require cleanup, where microbes isolated from oil rich environments like oil
wells, oil transfer pipelines...etc. have been found having the potential to
degrade oil or use it as an energy source. Thus they serve as a remedy to oil
spills.
15. APPLICATIONS
Humans have long been manipulating genetic material through:
• breeding
•modern genetic modification
• crop yield,
• sewage treatment
17. ROLE OF BIOTECHNOLOGY IN SOLVING
ENVIORMENTAL PROBLEMS :
•Man enviorment has deteriorated a lot with grievous consequences.
•Biological agents used such as organisms ,tissues, cells, or isolated enzymes to convert
raw materials to products of greater value or remove waste materials or reduce
enviormental pollution by producing enviormental friendly alternatives and used for
bioremediation of polluted enviorments (soil, air & water ).
•In-situ – bio-stimulation, bio-augumentation phyto –remedation, etc.
•Ex-situ – Activated sludge process for water and waste water treatment ,air purification
systems).
18. EXAMPLES :
Air purification
Bio remediation of polluted soil
and water
Reduction in
enviormental
pollution
•Enviormental
monitoring
19. Air purification :
•This can be achieved by photosynthesis which reduces the
carbondioxide concentration (green house &acid gas)
,afforestation (planting of trees )& activites of microalgae.
Bio remediation of polluted soil and water :
• Many M.o &Higher plants are used for bio adsorption and bio
accumulation of heavy metals like Hg,Pb,Cd etc.
•Bioremediation for higher plants as nicotiana glauca, brassica
junica ,mentha aquatica etc.
20. Reduction in enviormental pollution :
•Biotechnology can be used to mimimize the enviormental pollution by
producing enviormental friendly alternatives such as bio insecticides, bio-
fertilizers, bio degradable plastics , & bio energy.
21. Enviormental monitoring :
M.o, Micro algae, higher plants and other lower animals can be used for
enviormental monitoring .These are very sensitive to certain pollutants and
thus used a s pollution indicator organisms to identify and quantify the effects
of the pollutants.
22. BIOTECHNOLOGY MAKES THE WORLD MORE
SUSTAINABLE :
•As climate change looms over our future, many industries are turning to
biotechnology for solutions to make all aspects of our lives more
sustainable for the environment.
23. Bioplastics :
• Plastic pollution is one of the major environmental issues we’re currently
facing. The waste from petrochemical plastic production plants, as well as the
tonnes of non-biodegradable plastic that is thrown away daily, are huge
problems for the environment. New technologies that incorporate biology in
the production of plastics could offer a more sustainable alternative.
•Ex : coca-cola ,yogurt cups.
24.
25. Enzymatic detergents :
•Stronger and more sustainable detergents are one of the earliest applications of
industrial biotechnology.
•Back in the 60s, Danish biotech giant Novozymes started selling the first
enzymatic detergents.
•They consist of specialized enzymes obtained from microorganisms that are
able to break down molecules behind difficult stains, such as blood and fat.
And unlike chemical alternatives, enzymatic detergents are biodegradable.
A key advantage is that they can work at lower temperatures, therefore
reducing the amount of energy spent on washing clothes. In addition,
enzymatic detergents can be used to clean medical equipment more
thoroughly and efficiently than common cleaning solutions.
26. Biofuels :
•Fossil fuels are the biggest culprit behind air pollution, which is estimated to kill
millions of people each year. In recent years, biofuels produced from crops have
become an increasingly common alternative. However, these crops are starting to
compete for agricultural land, which can contribute to deforestation and rising
food prices.
27. Cultivated meat :
•The meat industry is a huge polluter. Biotechnology could significantly
reduce the use of land, water, and energy by growing meat without the
animal, directly from a small sample of muscle and fat cells. This approach
would also reduce the use of antibiotics in meat production as it can be
created in sterile lab conditions.
•In 2020, Singapore became the first country to approve the
commercialization of a cultivated meat product, developed by US-based
company Eat Just.
28.
29. Flavorings :
•Most flavorings were traditionally extracted from plants. Today, however, many of
them are produced through petrochemical processes. Biotechnology could provide
an environmentally friendly alternative that does not require as much land and
resources as traditional methods.
•For example, traditionally 160,000 oranges are needed to produce just a liter of the
orange flavoring molecule valencene. Instead, bacteria or yeast can be engineered to
produce these molecules in industrial vats, reliably producing large volumes of
virtually any flavoring. A leader in this field is Evolva, in Switzerland, which
produces the natural sweetener as well as orange, vanilla, and grapefruit flavors.
30. •Cosmetics
•Many natural cosmetics contain active ingredients sourced from plants. However, for some
of these ingredients, the amount obtained from a plant can be quite small compared to the
amount of land, water, and energy that are needed to produce it.
•Companies such as Bioeffect in Iceland or Biossance in the US are looking at producing
these compounds more sustainably through microbial fermentation. Using this fermentation
technology, the French biotech company Deinove is able to produce the anti-ageing
compound phytoene in its pure form, with the goal of using it as an ingredient for skincare
products. The firm also does research into new cosmetic ingredients by studying bacteria
that are able to live in the extreme conditions of hot water springs.
31. Clothing :
•Fast fashion is a big sustainability issue. Biotechnology could put a stop to its
environmental impact by replacing polluting chemical processes and making textile
waste recyclable and biodegradable. Enzymes are already used routinely to wash
and bleach clothing and to prevent wool from shrinking. New technologies could
allow us to go further by using microbes to produce textiles.
•That is the case of AM Silk in Germany, which uses bacterial fermentation to
produce spider silk fibers. Among the many applications of this material, the
company is working with Adidas to make a biodegradable running shoe that does
not leave waste behind.
32. CONCLUSION :
•Biotechnology Provides Clean Air, Water and Green Products. New
industrial and environmental biotechnology advances are helping to make
manufacturing processes cleaner and more efficient by reducing toxic
chemical pollution and greenhouse gas emissions.
“ Enviormental pollution is an incurable disease, it can only be prevented” .