Abstract— To understand the responses of various soybean (Glycine max) cultivars to ultraviolet-B, Dongnong 42, Zhonghuang 57 and Jin 36 were cultured either under UV-B radiation (10.08 kJ / m-2 UV-B) or without UV-B for 25 days. Under UV-B irradiation, significant difference were observed in plant height, nodes length, contents of soluble sugar, proline, protein, flavionoids, GSH, ASA, relative content of anthocyanins, also the emzymes activities of APX ,SOD and GR. The lowest plant height was presented in Jin 36, followed Zhonghuang 57 and Dongnong 42. The node length of plant decreased from the first node to the fourth one, and the maximal reduction was observed in Jin 36. Contents of protein, flavionoids, GSH, ASA and anthocyanins as well as emzyme activities of APX, SOD and GR significantly increased under UV-B irradiation where content of protein and activities of emzyme APX, SOD and GR were higher in Zhonghuang 57 than those in Dongnong 42 and Jin 36; higher GSH, ASA, flavonoids, as well as the anthocyanins were showed in Donghong 42. Increased proline content was observed in the three cultivars upon UV-B (p<0.05)>Dongnong 42 > Jin36; for the soluble sugar content, the order was Zhongnong 57>Jin 36>Dongnong 42 (p<0.05). Various soybean cultivars showed varied physiological responses upon UV-B.
— In the present work, impact of UV-B radiation (280-
315nm: 0.4 W m-2) on growth, photosynthetic pigments, protein,
ascorbate, proline and lipid peroxidation have been studied in
two cyanobacteria Nostoc muscorum and Synechocystis PCC
6803. UV-B radiation (2 to 6 hrs) leads to 55% inhibition of
growth in Synechocystis PCC 6803 in comparison to control
where as in Nostoc muscorum growth reduces up to 45%. This
UV-B treatment also significantly decreased the contents of
chlorophyll, carotenoids and phycocyanin. Photosynthetic
pigments decreased with increasing doses of UV-B (2 to 6 hrs)
radiation. However, the inhibitory effect in Synechocystis PCC
6803 was more pronounced than in Nostoc muscorum. With
increasing UV-B exposure period, production of ascorbate (19-
45%), proline (12-29%) and lipid peroxidation was significantly
higher in Synechocystis PCC 6803 as compared to control
sample. It was observed that lipid peroxidation enhanced 33 %
than control sample of Synechocystis PCC 6803. Our result shows
that photosynthetic apparatus is the main target of UV-B
radiation causing degradation of photosynthetic pigments. This
study concluded that Synechocystis PCC 6803 was the susceptible
organism for survival in stress condition than Nostoc muscorum.
Application of electrospun nanofibres in food processingsujayasree o.j
Electrospinning is a simple, versatile, and cost-effective technology which generates nano fibers with high surface area to volume ratio.Solution and processing parameters such as viscosity, molecular weight, concentration of the polymer, applied voltage, tip to collector distance, conductivity, etc. significantly affect the fiber morphology and by manipulation of these parameters one can get desired properties for specific application
Spun nano fibers are increasingly being used in a variety of applications such as, tissue engineering scaffolds, wound healing, drug delivery, immobilization of enzymes, as membrane in biosensors, protective clothing, cosmetics, affinity membranes, encapsulation of bioactive component, active packaging, filtration applications etc
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
Exploring the Mycobactericidal potential of vitamin C rich fruits TARUNSAROCH
Mycobacterium tuberculosis, the infective agent of tuberculosis(TB) Causes one of the world’s most devastating global health crisis
The treatment of drug-susceptible TB lasts more than six-months and require the use of drugs to achieve a cure, whereas for XDR treatment lasts for more than1 year
The two most effective first- line TB drugs for drug susceptible TB are isoniazid (INH) and rifampicin(RIF), which has to be taken for six months.
The long treatment period is of major concern as it leads to the development of the drug resistant TB.
The study found that anti-tuberculosis drugs killed more bacteria in laboratory when the mice received vitamin C.
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...iosrjce
Environmental impurities and degrading substances are major causes of pollution. On-going efforts
to cut the world’s carbon emission have been an uphill battle with the unknown. Because of the importance of
some photosynthetic bacteria, viable, efficient and environment-friendly biological remediation researches have
therefore become inevitably important. In search of these criteria, there was the need for the logical merger of
efficacy of the mop-up capability of the biological agents and productive beneficial end-results. We studied the
previously identified thermophilic species of photosynthetic bacterium, Rhodocista pekingensis isolated from a
local hot spring in Malaysia. Inductive Toxic-mopping (IT-m) technique was used to assess the production of
bacteriocarotenoids by metabolic bioconversion process of eight toxic chemical compounds by Rhodocista
pekingensis. Results showed a positive response to four of the toxic chemical compounds with the production of
65±5 mg/L, 63±3 mg/L, 59±3 mg/L and 53±6 mg/L yields of raw bacteriocarotenoid in DMSO, Methanol,
Isopropyl alcohol and Dubai crude respectively when compared with 65±3 mg/l of raw bacteriocarotenoid
produced in the Control experiment. Four of the toxic compounds were efficient to trigger carotenogenic
processes needed in the production of beneficial bacteriocarotenoids, two were mild and two others were
inadequate to trigger a substantial process. The improvement in carotenoid production with the assimilated
toxic chemicals corroborated the efficiency of the Inductive Toxic-mopping Bioremediation pattern and
beneficial convertibility of chemical wastes by Rhodocista pekingensis
— In the present work, impact of UV-B radiation (280-
315nm: 0.4 W m-2) on growth, photosynthetic pigments, protein,
ascorbate, proline and lipid peroxidation have been studied in
two cyanobacteria Nostoc muscorum and Synechocystis PCC
6803. UV-B radiation (2 to 6 hrs) leads to 55% inhibition of
growth in Synechocystis PCC 6803 in comparison to control
where as in Nostoc muscorum growth reduces up to 45%. This
UV-B treatment also significantly decreased the contents of
chlorophyll, carotenoids and phycocyanin. Photosynthetic
pigments decreased with increasing doses of UV-B (2 to 6 hrs)
radiation. However, the inhibitory effect in Synechocystis PCC
6803 was more pronounced than in Nostoc muscorum. With
increasing UV-B exposure period, production of ascorbate (19-
45%), proline (12-29%) and lipid peroxidation was significantly
higher in Synechocystis PCC 6803 as compared to control
sample. It was observed that lipid peroxidation enhanced 33 %
than control sample of Synechocystis PCC 6803. Our result shows
that photosynthetic apparatus is the main target of UV-B
radiation causing degradation of photosynthetic pigments. This
study concluded that Synechocystis PCC 6803 was the susceptible
organism for survival in stress condition than Nostoc muscorum.
Application of electrospun nanofibres in food processingsujayasree o.j
Electrospinning is a simple, versatile, and cost-effective technology which generates nano fibers with high surface area to volume ratio.Solution and processing parameters such as viscosity, molecular weight, concentration of the polymer, applied voltage, tip to collector distance, conductivity, etc. significantly affect the fiber morphology and by manipulation of these parameters one can get desired properties for specific application
Spun nano fibers are increasingly being used in a variety of applications such as, tissue engineering scaffolds, wound healing, drug delivery, immobilization of enzymes, as membrane in biosensors, protective clothing, cosmetics, affinity membranes, encapsulation of bioactive component, active packaging, filtration applications etc
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
Exploring the Mycobactericidal potential of vitamin C rich fruits TARUNSAROCH
Mycobacterium tuberculosis, the infective agent of tuberculosis(TB) Causes one of the world’s most devastating global health crisis
The treatment of drug-susceptible TB lasts more than six-months and require the use of drugs to achieve a cure, whereas for XDR treatment lasts for more than1 year
The two most effective first- line TB drugs for drug susceptible TB are isoniazid (INH) and rifampicin(RIF), which has to be taken for six months.
The long treatment period is of major concern as it leads to the development of the drug resistant TB.
The study found that anti-tuberculosis drugs killed more bacteria in laboratory when the mice received vitamin C.
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...iosrjce
Environmental impurities and degrading substances are major causes of pollution. On-going efforts
to cut the world’s carbon emission have been an uphill battle with the unknown. Because of the importance of
some photosynthetic bacteria, viable, efficient and environment-friendly biological remediation researches have
therefore become inevitably important. In search of these criteria, there was the need for the logical merger of
efficacy of the mop-up capability of the biological agents and productive beneficial end-results. We studied the
previously identified thermophilic species of photosynthetic bacterium, Rhodocista pekingensis isolated from a
local hot spring in Malaysia. Inductive Toxic-mopping (IT-m) technique was used to assess the production of
bacteriocarotenoids by metabolic bioconversion process of eight toxic chemical compounds by Rhodocista
pekingensis. Results showed a positive response to four of the toxic chemical compounds with the production of
65±5 mg/L, 63±3 mg/L, 59±3 mg/L and 53±6 mg/L yields of raw bacteriocarotenoid in DMSO, Methanol,
Isopropyl alcohol and Dubai crude respectively when compared with 65±3 mg/l of raw bacteriocarotenoid
produced in the Control experiment. Four of the toxic compounds were efficient to trigger carotenogenic
processes needed in the production of beneficial bacteriocarotenoids, two were mild and two others were
inadequate to trigger a substantial process. The improvement in carotenoid production with the assimilated
toxic chemicals corroborated the efficiency of the Inductive Toxic-mopping Bioremediation pattern and
beneficial convertibility of chemical wastes by Rhodocista pekingensis
Spectroscopic Characterization of Biofield Treated Metronidazole and TinidazoleMahendra Kumar Trivedi
Metronidazole and tinidazole are widely used antimicrobial drugs against Gram-negative and Gram-positive anaerobic bacteria. The present study was aimed to evaluate the impact of biofield treatment on metronidazole and tinidazole using FT-IR and UV spectroscopy. The study was carried out in two groups i.e. control and treatment. Treatment groups were subjected to Mr. Trivedi’s biofield treatment while no treatment was given to control group. FT-IR spectrum of treated metronidazole showed the impact of biofield treatment on frequency of characteristic functional groups such as C=C (imidazole ring) stretching was appeared at lower frequency i.e. from 1600 cm-1 to 1553 cm-1. Likewise, NO2 asymmetric stretching and C-N symmetric stretching were appeared at higher wave number i.e. 1479 cm-1 to 1501 cm-1 and 1070 cm-1 to 1077 cm-1, respectively. FT-IR spectrum of tinidazole showed shifting in absorption peak of C-N stretching to higher wavenumber from 1002 cm-1 (control) to 1022 cm-1. The wavenumber of aromatic C=C bond (in imidazole) was shifted to lower frequency, which could be due to increases in conjugation effect. Further, increases in wavenumber of NO2 and C-N in treated sample suggested the increased force constant and bond strength as compared to control. Because of higher conjugation effect and increased bond strength, the molecule supposed to be more stable. The UV spectra of both metronidazole and tinidazole showed the similar patterns of lambda max (λmax) with respect to their control. The FT-IR results of both drugs suggest that there was an impact of biofield treatment on atomic level of metronidazole and tinidazole, as compared to control.
Impact of Biofield Treatment on Atomic and Structural Characteristics of Bari...Mahendra Kumar Trivedi
In the present study, the barium titanate powder was subjected to biofield treatment. Further, the control and treated samples were characterized using X-ray diffraction (XRD) and Fourier transform infrared spectrometer (FT-IR) and Electron spin resonance (ESR).
Effects of β-cyclodextrin on in vitro rooting and bulbing of lilium (Lilium l...Premier Publishers
The auxins liability often limits expected effects on in vitro culture of plant tissues. In this regard, β-cyclodextrine was used as protective substance to auxins in order to improve rooting and in vitro bulbing of lily (Lilium longiflorum L.). This oligosaccharide was added at a concentration of 10, 20 and 30 mg/l to the rooting medium containing mineral and vitamin additives of Murashige and Skoog (MS), 1.5 mg/l of indole-3-butyric acid (IBA), 30 g/l of sucrose and 6 g/l of agar. After 30 days of culture, in order to enhance in vitro bulbe formation, a liquid medium supplemented with 6-benzylaminopuine (BAP) was added to form a double phase (solid / liquid) media. The results obtained showed that β-cyclodextrin improves the rooting of lily in vitro shoots. Indeed, the average number of roots increases from 5.64 to 7.20 roots per vitro-plant in the medium supplemented with β-cyclodextrin and root length increased from 1.72 to 2.2 cm compared to control.
Green synthesis of zinc oxide nano particles using flower extract cassia dens...IJERD Editor
Green synthesis of metal nanoparticles is an interesting issue of the nanoscience and
nanobiotechnology. There is a growing attention to biosynthesis the metal nanoparticles using organisms.
Among these organisms, plants seem to be the best and they are suitable for large scale biosynthesis of
nanoparticles. Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than that in
the case of other organisms. The present investigation was carried out to green synthesis of zinc oxide
nanoparticles by using the medicinal plant cassia densistipulata taub. The flower was collected from the campus
of Anantapuramu, Andhra Pradesh and their petals were separated. The petals were taken and cleaned with
dimeneralized water and soaked for an hour on dry cloth to remove moisture from the petals.
Synthesis of Zinc Nanoparticles was done by mixing 5gms of Zinc Nitrate with 50ml of aqueous
extract of cassia densistipulata taub petals. The formation of nanoparticles was monitored by visualizing color
changes and it was confirmed by Electron microscope (SEM), UV-Vis spectrophotometer and Fourier
Transform Infra-Red (FT-IR) spectroscopy. The results of various techniques confirmed the presence Zinc oxide
nanoparticles.
Food irradiation (the application of ionizing radiation to food) is a technology that improves the safety and extends the shelf life of foods by reducing or eliminating microorganisms and insects. Like pasteurizing milk and canning fruits and vegetables, irradiation can make food safer for the consumer
Curcumin extract nanoparticles: preparation, characterization and antimicrobi...Innspub Net
In recent years, synthesized zinc oxide nanoparticles have been increasingly investigated for different medicinal uses. In the present study, we aimed at the biosynthesis of zinc oxide using a curcumin extract. Although, toxic effects of curcumin derivative and zinc oxide nanoparticles in different concentration have been studied specifically on animal models besides the antibacterial activity of synthesized curcumin extract and zinc oxide nanoparticles. The aim of the study was to synthesize extract combined zinc oxide nanoparticles. Methods: The synthesized nanoparticles and extract were characterized for the particle size distribution, morphology, optical properties and surface charge by using UVvisible spectroscopy, dynamic light scattering (DLS), (TEM) and (SEM). Elemental composition and structural properties were studied by energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction spectroscopy (XRD). Results: The synthesized nanoparticles and curcumin were irregular shape and had a size distribution in the range of 50–100 nm. The in vitro toxicity effects of zinc oxide and extract showed no toxic effect with different concentration with antibacterial effect.
Synthesis and Evaluation of the Cytotoxic and Anti-Proliferative Properties o...IJEAB
Doxorubicin (Dox) is a potent chemotherapeutic agent used in the treatment of cancer. In the present study, pH responsive chitosan polymer coated Dox nanoparticle (Composite) was developed to investigate targeted drug delivery against breast cancer. The anticancer drug DOX-ZnO QDs was loaded to the chitosan nanoparticles. The synthesized free and drug loaded nanoparticle were analyzed using Fourier transmission electron microscopy (FTIR) and UV-Visible spectroscopy(UV-Vis). The particle size was measured using Transmission Electron Microscopy (TEM). Further, the composite was evaluated for its anticancer effects. Drug release analysis showed significantly larger amount of drug released in acidic pH of 5.0 compared to pH 7.4. The composite was significantly more cytotoxic to the breast cancer cells MCF-7 and SKBR-3. The composite was however, less toxic to HEK-293 human embryonic kidney cells confirming minimum side effects on normal cells andcytotoxic to tumor cells. DAPI staining showed nuclear degradation in composite treated breast cancer cells. The cellular uptake of the composite was analyzed by confocal microscopy. The composite induced a G0/G1 phase arrest in breast cancer cells and the number of colonies formed by the composite treated breast cancer cells formed less number of colonies compared to free NP. Our results showed that our composite could serve as a promising therapeutic approach to improve clinical outcomes against various malignancies.
It an overall view on two research papers. Biological synthesis of Nano particles from plants and microorganisms
and the synthesis of metallic Nano particles using plant extract
Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract a...ijtsrd
The main objective of the present study is to deal with the green synthesis of Zinc Oxide [ZnO] nanoparticles utilizing aqueous leaf extract of Jasminum Fluminense.` Zinc acetate [Zn(O2CCH3)2(H2O)2] and sodium hydroxide [NaOH] were used as starting materials and Jasminum Fluminense [Nithyamalli] leaf extract is used as precursor in the synthesis of ZnO nanoparticles. The resultant nanopowder was characterized by using Ultraviolet [UV] “ Visible Spectroscopy, Fourier Transform Infrared Spectroscopy [FTIR] and Transmission Electron Microscopic [TEM] studies. Formation of ZnO nanoparticles has been confirmed by UV-visible spectroscopy and the TEM analysis spectacles that the synthesized ZnO nanoparticles are of face centered cubic (fcc) structure and the size is found to be around 20 nm. FTIR spectral analysis indicated the leaf extract acts as the reducing and capping agents on the surface of ZnO nanoparticles. This simplistic and green approach may provide a useful tool to hefty extent in the synthesis of ZnO nanoparticles. These synthesized nanostructures illustrate novel applications in many fields such as cosmetics, optoelectronics, sensors, transducers and biomedical science because it is environmentally friendly and does not involve any harmful substances. S. Amudha"Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract and its Spectral and Optical Characterization Studies" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd12908.pdf http://www.ijtsrd.com/physics/nanotechnology/12908/green-synthesis-of-zno-nanoparticles-using-jasminum-fluminense-leaf-extract-and-its-spectral-and-optical-characterization-studies/s-amudha
Biosynthesis and Characterization of Zinc Oxide Nanoparticles using Onion Bul...ijtsrd
The wide application of nanoparticles stimulates the need for synthesizing them but, the conventional methods are usually hazardous and energy consuming. This leads to focus on œgreen synthesis of nanoparticles which seems to be easy efficient and ecofriendly approach. In this study, the plant mediated synthesis of zinc oxide nanoparticles (ZnO NPs) was carried out using bulb extract of Allium cepa as a reducing agent. The optimized nano zinc thus obtained was quantified and characterized using UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray diffraction, Scanning Electron Microscope (SEM), EDAX and Zeta potential analyses. Further, the synthesized ZnO NPs were tested for antimicrobial activity. N. Tensingh Baliah | S. Lega Priyatharsini"Biosynthesis and Characterization of Zinc Oxide Nanoparticles using Onion Bulb Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd8305.pdf http://www.ijtsrd.com/biological-science/botany/8305/biosynthesis-and-characterization-of-zinc-oxide--nanoparticles-using-onion-bulb-extract/n-tensingh-baliah
Degradation and Microbiological Validation of Meropenem Antibiotic in Aqueous...IJERA Editor
Aqueous UV, UV/H2O2, UV/TiO2 and UV/TiO2/H2O2mediateddegradation/oxidation of the carbapenem antibiotic, meropenem (MERO) was experimentally studied. Degussa P-25 titanium dioxide was used as photocatalyst and UV-light source was used for activation of TiO2.The nanosized titanium dioxide was immobilized on the glass support for improving the efficiency and economics of the photocatalytic processes. The immobilized film of titanium dioxide has been characterized, using X-ray diffraction (XRD) andscanning electron microscopy (SEM).The study of antibiotic degradation was conducted in the specific Batch Photocatalytic Reactor. MERO standard solution was used at 500 μg/ml concentration, which degraded up to 99% of antibiotics. Microbiological assay showed that the loss of antibacterial activity is directly proportional to the time of UV-irradiation. The experiment also showed that the UV-irradiation itself causes the degradation of antibiotics, but in very slow manner in comparison to the photocatalysis process. The experimental study showed that UV/TiO2/H2O2 system is effective and efficient for the treatment of antibiotic waste.
Photosensitization is a treatment involving the interaction of the two nontoxic factors, photoactive compound and visible light, which in the presence of oxygen results in the selective destruction of the target cell.
Different micro‐organisms, such as multidrug‐resistant bacteria, yeasts, microfungi and viruses, are susceptible to this treatment.
Characterization of Manganese doped ZnO (MZO) thin films by Spin Coating Tech...IOSR Journals
Doping is a widely used to improve the structural and optical properties of semiconductors. However deposition route is also very important to get nanostructure with different properties. ZnO nanostructures doped with Mn having 5% doping concentrations by weight percentage have been synthesized in the laboratory using Spin coating technique. Scanning Electron Microscope (SEM) image shows the around one millimeter and X-ray diffractometer studies shows that the average diameter of the particles is 25 nm. From the UV-Vis studies the annealing temperature increases the crystal size decreases and the bandgap values increases accordingly.
Plant Mediated Synthesis of ZnO and Mn Doped ZnO Nanoparticles Using Carica P...IIJSRJournal
In this work, Zinc Oxide (ZnO) and Mn-doped ZnO nanoparticles were green synthesized using Carica papaya extract by the Co-precipitation method. X-ray diffraction (XRD) results revealed the formation of ZnO and Mn-doped ZnO nanoparticles with the wurtzite crystal structure (hexagonal). Due to the presence of dopant Manganese (Mn) the optical spectra showed a redshift in the absorbance spectrum. Structural and optical properties of the end product showed that the manganese ions (Mn2+) substituted the Zinc ions (Zn2+) without altering the Wurtzite structure of ZnO. Fourier Transform Infrared Spectroscopy (FTIR) spectra confirm the presence of metal oxide present in the end product. The antibacterial efficiency of ZnO and Mn-doped ZnO nanoparticles were studied using the agar well diffusion method against Gram-positive and Gram–negative bacteria. It is obvious from the results that Mn doped ZnO nanoparticles exhibit better antibacterial activity than ZnO nanoparticles.
Spectroscopic Characterization of Biofield Treated Metronidazole and TinidazoleMahendra Kumar Trivedi
Metronidazole and tinidazole are widely used antimicrobial drugs against Gram-negative and Gram-positive anaerobic bacteria. The present study was aimed to evaluate the impact of biofield treatment on metronidazole and tinidazole using FT-IR and UV spectroscopy. The study was carried out in two groups i.e. control and treatment. Treatment groups were subjected to Mr. Trivedi’s biofield treatment while no treatment was given to control group. FT-IR spectrum of treated metronidazole showed the impact of biofield treatment on frequency of characteristic functional groups such as C=C (imidazole ring) stretching was appeared at lower frequency i.e. from 1600 cm-1 to 1553 cm-1. Likewise, NO2 asymmetric stretching and C-N symmetric stretching were appeared at higher wave number i.e. 1479 cm-1 to 1501 cm-1 and 1070 cm-1 to 1077 cm-1, respectively. FT-IR spectrum of tinidazole showed shifting in absorption peak of C-N stretching to higher wavenumber from 1002 cm-1 (control) to 1022 cm-1. The wavenumber of aromatic C=C bond (in imidazole) was shifted to lower frequency, which could be due to increases in conjugation effect. Further, increases in wavenumber of NO2 and C-N in treated sample suggested the increased force constant and bond strength as compared to control. Because of higher conjugation effect and increased bond strength, the molecule supposed to be more stable. The UV spectra of both metronidazole and tinidazole showed the similar patterns of lambda max (λmax) with respect to their control. The FT-IR results of both drugs suggest that there was an impact of biofield treatment on atomic level of metronidazole and tinidazole, as compared to control.
Impact of Biofield Treatment on Atomic and Structural Characteristics of Bari...Mahendra Kumar Trivedi
In the present study, the barium titanate powder was subjected to biofield treatment. Further, the control and treated samples were characterized using X-ray diffraction (XRD) and Fourier transform infrared spectrometer (FT-IR) and Electron spin resonance (ESR).
Effects of β-cyclodextrin on in vitro rooting and bulbing of lilium (Lilium l...Premier Publishers
The auxins liability often limits expected effects on in vitro culture of plant tissues. In this regard, β-cyclodextrine was used as protective substance to auxins in order to improve rooting and in vitro bulbing of lily (Lilium longiflorum L.). This oligosaccharide was added at a concentration of 10, 20 and 30 mg/l to the rooting medium containing mineral and vitamin additives of Murashige and Skoog (MS), 1.5 mg/l of indole-3-butyric acid (IBA), 30 g/l of sucrose and 6 g/l of agar. After 30 days of culture, in order to enhance in vitro bulbe formation, a liquid medium supplemented with 6-benzylaminopuine (BAP) was added to form a double phase (solid / liquid) media. The results obtained showed that β-cyclodextrin improves the rooting of lily in vitro shoots. Indeed, the average number of roots increases from 5.64 to 7.20 roots per vitro-plant in the medium supplemented with β-cyclodextrin and root length increased from 1.72 to 2.2 cm compared to control.
Green synthesis of zinc oxide nano particles using flower extract cassia dens...IJERD Editor
Green synthesis of metal nanoparticles is an interesting issue of the nanoscience and
nanobiotechnology. There is a growing attention to biosynthesis the metal nanoparticles using organisms.
Among these organisms, plants seem to be the best and they are suitable for large scale biosynthesis of
nanoparticles. Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than that in
the case of other organisms. The present investigation was carried out to green synthesis of zinc oxide
nanoparticles by using the medicinal plant cassia densistipulata taub. The flower was collected from the campus
of Anantapuramu, Andhra Pradesh and their petals were separated. The petals were taken and cleaned with
dimeneralized water and soaked for an hour on dry cloth to remove moisture from the petals.
Synthesis of Zinc Nanoparticles was done by mixing 5gms of Zinc Nitrate with 50ml of aqueous
extract of cassia densistipulata taub petals. The formation of nanoparticles was monitored by visualizing color
changes and it was confirmed by Electron microscope (SEM), UV-Vis spectrophotometer and Fourier
Transform Infra-Red (FT-IR) spectroscopy. The results of various techniques confirmed the presence Zinc oxide
nanoparticles.
Food irradiation (the application of ionizing radiation to food) is a technology that improves the safety and extends the shelf life of foods by reducing or eliminating microorganisms and insects. Like pasteurizing milk and canning fruits and vegetables, irradiation can make food safer for the consumer
Curcumin extract nanoparticles: preparation, characterization and antimicrobi...Innspub Net
In recent years, synthesized zinc oxide nanoparticles have been increasingly investigated for different medicinal uses. In the present study, we aimed at the biosynthesis of zinc oxide using a curcumin extract. Although, toxic effects of curcumin derivative and zinc oxide nanoparticles in different concentration have been studied specifically on animal models besides the antibacterial activity of synthesized curcumin extract and zinc oxide nanoparticles. The aim of the study was to synthesize extract combined zinc oxide nanoparticles. Methods: The synthesized nanoparticles and extract were characterized for the particle size distribution, morphology, optical properties and surface charge by using UVvisible spectroscopy, dynamic light scattering (DLS), (TEM) and (SEM). Elemental composition and structural properties were studied by energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction spectroscopy (XRD). Results: The synthesized nanoparticles and curcumin were irregular shape and had a size distribution in the range of 50–100 nm. The in vitro toxicity effects of zinc oxide and extract showed no toxic effect with different concentration with antibacterial effect.
Synthesis and Evaluation of the Cytotoxic and Anti-Proliferative Properties o...IJEAB
Doxorubicin (Dox) is a potent chemotherapeutic agent used in the treatment of cancer. In the present study, pH responsive chitosan polymer coated Dox nanoparticle (Composite) was developed to investigate targeted drug delivery against breast cancer. The anticancer drug DOX-ZnO QDs was loaded to the chitosan nanoparticles. The synthesized free and drug loaded nanoparticle were analyzed using Fourier transmission electron microscopy (FTIR) and UV-Visible spectroscopy(UV-Vis). The particle size was measured using Transmission Electron Microscopy (TEM). Further, the composite was evaluated for its anticancer effects. Drug release analysis showed significantly larger amount of drug released in acidic pH of 5.0 compared to pH 7.4. The composite was significantly more cytotoxic to the breast cancer cells MCF-7 and SKBR-3. The composite was however, less toxic to HEK-293 human embryonic kidney cells confirming minimum side effects on normal cells andcytotoxic to tumor cells. DAPI staining showed nuclear degradation in composite treated breast cancer cells. The cellular uptake of the composite was analyzed by confocal microscopy. The composite induced a G0/G1 phase arrest in breast cancer cells and the number of colonies formed by the composite treated breast cancer cells formed less number of colonies compared to free NP. Our results showed that our composite could serve as a promising therapeutic approach to improve clinical outcomes against various malignancies.
It an overall view on two research papers. Biological synthesis of Nano particles from plants and microorganisms
and the synthesis of metallic Nano particles using plant extract
Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract a...ijtsrd
The main objective of the present study is to deal with the green synthesis of Zinc Oxide [ZnO] nanoparticles utilizing aqueous leaf extract of Jasminum Fluminense.` Zinc acetate [Zn(O2CCH3)2(H2O)2] and sodium hydroxide [NaOH] were used as starting materials and Jasminum Fluminense [Nithyamalli] leaf extract is used as precursor in the synthesis of ZnO nanoparticles. The resultant nanopowder was characterized by using Ultraviolet [UV] “ Visible Spectroscopy, Fourier Transform Infrared Spectroscopy [FTIR] and Transmission Electron Microscopic [TEM] studies. Formation of ZnO nanoparticles has been confirmed by UV-visible spectroscopy and the TEM analysis spectacles that the synthesized ZnO nanoparticles are of face centered cubic (fcc) structure and the size is found to be around 20 nm. FTIR spectral analysis indicated the leaf extract acts as the reducing and capping agents on the surface of ZnO nanoparticles. This simplistic and green approach may provide a useful tool to hefty extent in the synthesis of ZnO nanoparticles. These synthesized nanostructures illustrate novel applications in many fields such as cosmetics, optoelectronics, sensors, transducers and biomedical science because it is environmentally friendly and does not involve any harmful substances. S. Amudha"Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract and its Spectral and Optical Characterization Studies" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd12908.pdf http://www.ijtsrd.com/physics/nanotechnology/12908/green-synthesis-of-zno-nanoparticles-using-jasminum-fluminense-leaf-extract-and-its-spectral-and-optical-characterization-studies/s-amudha
Biosynthesis and Characterization of Zinc Oxide Nanoparticles using Onion Bul...ijtsrd
The wide application of nanoparticles stimulates the need for synthesizing them but, the conventional methods are usually hazardous and energy consuming. This leads to focus on œgreen synthesis of nanoparticles which seems to be easy efficient and ecofriendly approach. In this study, the plant mediated synthesis of zinc oxide nanoparticles (ZnO NPs) was carried out using bulb extract of Allium cepa as a reducing agent. The optimized nano zinc thus obtained was quantified and characterized using UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray diffraction, Scanning Electron Microscope (SEM), EDAX and Zeta potential analyses. Further, the synthesized ZnO NPs were tested for antimicrobial activity. N. Tensingh Baliah | S. Lega Priyatharsini"Biosynthesis and Characterization of Zinc Oxide Nanoparticles using Onion Bulb Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd8305.pdf http://www.ijtsrd.com/biological-science/botany/8305/biosynthesis-and-characterization-of-zinc-oxide--nanoparticles-using-onion-bulb-extract/n-tensingh-baliah
Degradation and Microbiological Validation of Meropenem Antibiotic in Aqueous...IJERA Editor
Aqueous UV, UV/H2O2, UV/TiO2 and UV/TiO2/H2O2mediateddegradation/oxidation of the carbapenem antibiotic, meropenem (MERO) was experimentally studied. Degussa P-25 titanium dioxide was used as photocatalyst and UV-light source was used for activation of TiO2.The nanosized titanium dioxide was immobilized on the glass support for improving the efficiency and economics of the photocatalytic processes. The immobilized film of titanium dioxide has been characterized, using X-ray diffraction (XRD) andscanning electron microscopy (SEM).The study of antibiotic degradation was conducted in the specific Batch Photocatalytic Reactor. MERO standard solution was used at 500 μg/ml concentration, which degraded up to 99% of antibiotics. Microbiological assay showed that the loss of antibacterial activity is directly proportional to the time of UV-irradiation. The experiment also showed that the UV-irradiation itself causes the degradation of antibiotics, but in very slow manner in comparison to the photocatalysis process. The experimental study showed that UV/TiO2/H2O2 system is effective and efficient for the treatment of antibiotic waste.
Photosensitization is a treatment involving the interaction of the two nontoxic factors, photoactive compound and visible light, which in the presence of oxygen results in the selective destruction of the target cell.
Different micro‐organisms, such as multidrug‐resistant bacteria, yeasts, microfungi and viruses, are susceptible to this treatment.
Characterization of Manganese doped ZnO (MZO) thin films by Spin Coating Tech...IOSR Journals
Doping is a widely used to improve the structural and optical properties of semiconductors. However deposition route is also very important to get nanostructure with different properties. ZnO nanostructures doped with Mn having 5% doping concentrations by weight percentage have been synthesized in the laboratory using Spin coating technique. Scanning Electron Microscope (SEM) image shows the around one millimeter and X-ray diffractometer studies shows that the average diameter of the particles is 25 nm. From the UV-Vis studies the annealing temperature increases the crystal size decreases and the bandgap values increases accordingly.
Plant Mediated Synthesis of ZnO and Mn Doped ZnO Nanoparticles Using Carica P...IIJSRJournal
In this work, Zinc Oxide (ZnO) and Mn-doped ZnO nanoparticles were green synthesized using Carica papaya extract by the Co-precipitation method. X-ray diffraction (XRD) results revealed the formation of ZnO and Mn-doped ZnO nanoparticles with the wurtzite crystal structure (hexagonal). Due to the presence of dopant Manganese (Mn) the optical spectra showed a redshift in the absorbance spectrum. Structural and optical properties of the end product showed that the manganese ions (Mn2+) substituted the Zinc ions (Zn2+) without altering the Wurtzite structure of ZnO. Fourier Transform Infrared Spectroscopy (FTIR) spectra confirm the presence of metal oxide present in the end product. The antibacterial efficiency of ZnO and Mn-doped ZnO nanoparticles were studied using the agar well diffusion method against Gram-positive and Gram–negative bacteria. It is obvious from the results that Mn doped ZnO nanoparticles exhibit better antibacterial activity than ZnO nanoparticles.
Effects of LED light spectra on active oxygen metabolism and expression of an...Agriculture Journal IJOEAR
Abstract— The effects of various LEDs on active oxygen metabolism and patterns of SOD, POD and CAT isozymes in Houttuynia cordata Thunb. seedlings were investigated. After three weeks of light treatment, the MDA content was higher under blue LED compared with the control (P﹤0.05), while it decreased under white, red and yellow LEDs (P﹤0.05). The content of H2O2 was gradually increased in red, yellow, green and blue LEDs. The production rate of superoxide anion increased under yellow and blue LEDs by contrast with the control (P﹤0.05), and it decreased under white LED (P﹤0.05). LEDs altered the banding patterns of POD enzymes where the more loci of POD isozymes were observed under green and blue LEDs. The increased intensities of Fe-SOD were showed in green and blue LEDs. As for Mn-SOD and CAT enzymes, enhanced intensities appeared in all LED groups compared with the control. Our results indicated that the antioxidant system of Houttuynia cordata seedlings were more sensitive to short light wavelength than the long ones.
ABSTRACT: The Study was undertaken with an objective to develop a protocol for micropropagation of Pongamia pinnata pierre through shoot apex segments shoot of 0.5 to 1.0 cm were collected and used as a explant. The treatment of 1.0 NaOCl (Sodium hypochloride) (W/v) solution 1 minute to 10 minute time duration. These treated explant washed trice with double distilled water and cultured in MS (Murashige and skoog) medium. In this experiment auxin 2, 4-D, NAA and cytokinin BAP, Kinetin were used for optimization of maximum callus induction.
Shoot apex explant culturing callus induction maximum callus is produced when MS medium with 3.0 mg/l, 2, 4-D and BAP 0.5 mg/l, the optimized physical condition has to be maintain throughout the experiment. In this study about 30 to 35% mature sotmatic embryos germinated after sub culture from shoot apex. Different concentration and combination of NAA, IAA, IBA and BAP were used to inducted rooting on MS based medium. When the hight in vitro shoot, were reached up to 8 cm with healthy shooted roots, the plants were ready for hardening. The complete protocol for somatic embryogenesis, shoot induction, root induction up to hardening.
Determination of 8-Hydroxy-2 Deoxyguanosine in Pseudomonas Fluorescens Freeze...Agriculture Journal IJOEAR
Abstract— Oxidative DNA damage is involved in the f cell death induced by freeze-dried powder during storage. Cell 8-hydroxy-2’deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the “freeze-dried bacteria” oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in cell freeze-dried samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 0.5 µmol L-1 to 1.0 nmol L-1, the calibration curve was linear (r2=0.9995) and the limit of detection was 0.05 µmol L-1. The used method did not allow highlighting the presence in the samples of the 8OH within the limits of detection. A more successful method (more sensitive) would be needed to detect possibly the 8OH.
Phytochemical Studies on Linum Usitatissimum Seeds and the Nanoformulation of...Editor IJCATR
The phytochemical investigation of Linum usitatissimum, family Linaceae, resulted in the separation and
identification of a lignane, 8–5' neolignan 1 (dehydrodiconiferyl alcohol-4-β-D-glucoside), in addition to
identification of many compounds by the GC/MS technique. The antimicrobial activities of hexane, methylene
chloride and butanol fractions were comparable to ampicillin. The activities against E. coli were 29.2%, 37.5%,
and 66.7%, respectively; against S. aureus were 45.4%, 36.4% and 63.6%, respectively, and against C. albicans
were 26.9%, 46.1% and 73.1%, respectively. Antioxidant activity was assessed by ABTS method. It decreased
in the following order: ascorbic acid > butanol fraction > methylene chloride fraction > hexane fraction. The
cytotoxicity against HePG2 was found to be “moderate” for butanol and methylene chloride fractions, and
“weak” for the hexane fraction. The cytotoxicity against MCF-7 was found to be “strong” for butanol fraction,
and “weak” for both hexane and methylene chloride fractions. The lignane-rich subfraction, Lu 3d was
incorporated into pluronic nano-micelles using nanoprecipitation technique through a modified procedure.
The physicochemical characteristics of the developed Lu 3d-loaded nano-micelles such as particle size,
potential and morphology were determined using DLS and HR-TEM. The average diameters of the prepared
plain and Lu 3d-loaded pluronic nano-micelles were found to be 207 ± 12 and 225 ± 18 nm, respectively. The
encapsulation of Lu 3d into nano-sized particles has enhanced their aqueous dissolution and consequently
improved their bioavailability. Nanoformulation of Lu 3d led also to a stable colloidal dispersion with a strong
green color, indicating its homogenous distribution in the aqueous medium at a significantly higher
concentration than that obtained using other solvents.
Effects of Fungicides for Non Target Fungi Alternaria cassiaeIJEAB
The fungicides are used to control of pathogenic fungi in several tilth but they can affect negatively the microorganisms diversity of soil. The aim of this research was to evaluate the toxicity and environmental risk of tebuconazoles: captan, tebuconazole and the mixture chlorothalonil + propamocarb hidrochloride for fungi Alternaria cassiae. Each fungicide were performed three experiments in completely randomized design with three repetitions and the growth was evaluated daily. Inhibition concentration (IC50;7d) of tebuconazole was 3.49 mg L-1, the captan was 47.36 mg L-1 and of mixture chlorothalonil + propamocarb hidrochloride, 64.04 mg L-1. Tebuconazole is classified as moderately toxic and sensitivity, captan, low toxicity and sensitivity and the mixture, non toxic and insensitive but only captan showed possibility of adverse effect for A. cassiae.`
Effects of Fungicides for Non Target Fungi Alternaria cassiaeIJEAB
The fungicides are used to control of pathogenic fungi in several tilth but they can affect negatively the microorganisms diversity of soil. The aim of this research was to evaluate the toxicity and environmental risk of tebuconazoles: captan, tebuconazole and the mixture chlorothalonil + propamocarb hidrochloride for fungi Alternaria cassiae. Each fungicide were performed three experiments in completely randomized design with three repetitions and the growth was evaluated daily. Inhibition concentration (IC50;7d) of tebuconazole was 3.49 mg L-1, the captan was 47.36 mg L-1 and of mixture chlorothalonil + propamocarb hidrochloride, 64.04 mg L-1. Tebuconazole is classified as moderately toxic and sensitivity, captan, low toxicity and sensitivity and the mixture, non toxic and insensitive but only captan showed possibility of adverse effect for A. cassiae.
Application of Electrospun nanofibresin food industrysujayasree o.j
Electrospinning is a simple, versatile, and cost-effective technology which generates nano fibers with high surface area to volume ratio
Solution and processing parameters such as viscosity, molecular weight, concentration of the polymer, applied voltage, tip to collector distance, conductivity, etc. significantly affect the fiber morphology and by manipulation of these parameters one can get desired properties for specific application
Spun nano fibers are increasingly being used in a variety of applications such as, tissue engineering scaffolds, wound healing, drug delivery, immobilization of enzymes, as membrane in biosensors, protective clothing, cosmetics, affinity membranes, encapsulation of bioactive component, active packaging, filtration applications etc.
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
In vitro shoot multiplication in okra (Ablemonschus esculentues).TAJNE SACHIN
1: In vitro shoot multiplication in okra (Ablemonschus esculentues).
2: About Okra Plant.
3: Application of Tissue culture in Okra.
4: Objective.
5: Material and Method.
6: Methodology.
7: Treatment schedule.
8: Result of Program
9: Conclusion
10:References
Toxic Effect of Glyphosate-Pesticide on Lipid Peroxidation Superoxide Dismuta...Scientific Review SR
The oxidative stress indices lipid peroxidation (LPO), superoxide dismutase (SOD) and catalase (CAT) in juvenile Clarias gariepinus (average weight 200.15 g) exposed to sub - lethal dose 2.40mg/L and 4.98mg/L of glyphosate was investigated over a period of days 1,5,10 and 15 in three replicates. The colorimetric analysis showed increase in lipid peroxidation from 4.55 ±2.14a1 to 12.12± 10.00a1at 2.40mg/L but remain the same at 4.98mg/L (4.55±2.14a1) compared with control (3.03±0.01a1 to 1.51±2.14b1) from day 1 to 15. The SOD activity decreased significantly with time and concentration compared with control. The Catalase activity at day 15 decreased to 0.17±0.05a1 in 2.40mg/L but further increased to 0.28±0.05b1 in 4.98mg/L compared to 0.28±0.02a1 catalase activity as control. The result suggests that glyphosate induce oxidative stress that may overwhelm the antioxidant system in juvenile catfish especially at higher concentrations with long exposure.
preparation and foliar application of oligochitosanIJEAB
Oligochitosan with weight average molecu-lar weight (Mw) of 5000 g/mol was prepared by gamma Co-60 radiation degradation of 4% chitosan solution containing 0.5% H2O2 at 21 kGy. Nanosilica with size of 10 – 30 nm was synthesized by calcination of acid treated rice husk at 700o C for 2 h. The mixture of 2% oligo-chitosan-2% nanosilica was prepared by dispersion of nanosilica in oligochitosan solution. Oligochitosan, nanosilica and their mixture were characterized by gel permeation chromatography (GPC), transmission electr-on microscopy (TEM), X-ray diffraction (XRD), energy dispersive x-ray spectroscopy (EDX), Ultraviolet-visible spectroscopy (UV-Vis), and Furrier transform infrared spectroscopy (FT-IR). Effect of foliar application of oli-gochitosan and oligochitosan-nanosilica on soybean seed yield was conducted in experimental field. Results indi-cated that soybean seed yield increased 10.5 and 17.0% for oligochitosan and oligochitosan-nanosilica, respect-tively for the control. Radiation degraded oligo-chitosan and its mixture with nanosilica can be potentially used for cultivation of soybean with enhanced seed yield.
Use of stable and radio isotopes to understand the plant physiological processRAHUL GOPALE
Introduction
what is isotope ?
Types of Isotopes
Isotopic Labelling
ADVANTAGES AND DISADVANTAGES OF ISOTOPIC STUDY
APPLICATIONS OF ISOTOPES IN AGRICULTURE
Principle isotopes used in plant-soil studies
Case studies
FUTURE THRUSTS OF ISOTOPIC STUDY
CONCLUSIONS
REFERENCES
Similar to Responses of three soybean cultivars exposed to UV-B radiation (20)
Willie Nelson Net Worth: A Journey Through Music, Movies, and Business Venturesgreendigital
Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
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Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
Our Instagram channel:
@kuddlelifefoundation
Our Linkedin Page:
https://www.linkedin.com/company/kuddlelifefoundation/
and write to us if you have any questions:
info@kuddlelife.org
UNDERSTANDING WHAT GREEN WASHING IS!.pdfJulietMogola
Many companies today use green washing to lure the public into thinking they are conserving the environment but in real sense they are doing more harm. There have been such several cases from very big companies here in Kenya and also globally. This ranges from various sectors from manufacturing and goes to consumer products. Educating people on greenwashing will enable people to make better choices based on their analysis and not on what they see on marketing sites.
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
Prevalence of Toxoplasma gondii infection in domestic animals in District Ban...Open Access Research Paper
Toxoplasma gondii is an intracellular zoonotic protozoan parasite, infect both humans and animals population worldwide. It can also cause abortion and inborn disease in humans and livestock population. In the present study total of 313 domestic animals were screened for Toxoplasma gondii infection. Of which 45 cows, 55 buffalos, 68 goats, 60 sheep and 85 shaver chicken were tested. Among these 40 (88.88%) cows were negative and 05 (11.12%) were positive. Similarly 55 (92.72%) buffalos were negative and 04 (07.28%) were positive. In goats 68 (98.52%) were negative and 01 (01.48%) was recorded positive. In sheep and shaver chicken the infection were not recorded.
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
Responses of three soybean cultivars exposed to UV-B radiation
1. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-2, February- 2016]
Page | 149
Responses of three soybean cultivars exposed to UV-B radiation
Ning Gao1
, Liyan Yang2*
1,2*
College of Life Science, Shanxi Normal University, Linfen, Shanxi 041004, China
Abstract— To understand the responses of various soybean (Glycine max) cultivars to ultraviolet-B, Dongnong 42,
Zhonghuang 57 and Jin 36 were cultured either under UV-B radiation (10.08 kJ / m-2 UV-B) or without UV-B for 25 days.
Under UV-B irradiation, significant difference were observed in plant height, nodes length, contents of soluble sugar,
proline, protein, flavionoids, GSH, ASA, relative content of anthocyanins, also the emzymes activities of APX ,SOD and GR.
The lowest plant height was presented in Jin 36, followed Zhonghuang 57 and Dongnong 42. The node length of plant
decreased from the first node to the fourth one, and the maximal reduction was observed in Jin 36. Contents of protein,
flavionoids, GSH, ASA and anthocyanins as well as emzyme activities of APX, SOD and GR significantly increased under
UV-B irradiation where content of protein and activities of emzyme APX, SOD and GR were higher in Zhonghuang 57 than
those in Dongnong 42 and Jin 36; higher GSH, ASA, flavonoids, as well as the anthocyanins were showed in Donghong 42.
Increased proline content was observed in the three cultivars upon UV-B (p<0.05) where Zhonghuang 57 >Dongnong 42 >
Jin36; for the soluble sugar content, the order was Zhongnong 57>Jin 36>Dongnong 42 (p<0.05). Various soybean
cultivars showed varied physiological responses upon UV-B.
Keywords— UV-B, soybean, cultivars, physiological characters, response.
I. INTRODUCTION
Ultraviolet-B (wavelengths from 280 to 320 nm) radiance resulting from depletion in the stratospheric ozone layer affects
plant growth and metabolism with morphological, physiological, and biochemical changes is well documented (1). UV-B is
recognized as one of the major environmental regulators that control gene expression, celluar and metabolic activities (2).
Plants have adopted the specific ways to deal with UV-B radiation. Leaf thickening is one mechanism which may mitigate
the detrimental effects of UV-B by greater tissue absorption (3). Epidermal flavonoid accumulation is known as another
adaptive mechanism which can alleviate harm on mesophyll that related to photosynthesis (4, 5). Enzymatic and non-
enzymatic antioxidants that defense reactive oxygen species (ROS) induced by enhanced UV-B radiation (6, 7) has been
illustrated too (8). UVR8, plant photoreceptor of UV-B, was a newly discovered mechanism which involved in the UV-B
induced pathway (9-11).
Different species showed various responses, so is the cultivars of the same species (12, 13). It has been well established that
dicotyledonous plants are more sensitive to UV-B than monocotyledons. At the present study, three soybean cultivars
(Glycine max) were exposed to UV-B radiation, the growth and physiological traits were investigated.
II. MATERIAL AND METHOD
2.1 Plant material and treatment
Soybean (Glycine max) seeds were provided by the Wheat Research Institute, Shanxi Academy of Agricultural Sciences
(SAAS), China. They are selected for uniform size and sterilized for 10 min with 0.1% HgCl2 and washed for 20 min with
running water. Five seeds were sow in pots (15×20cm), and there were 6 treatments, with 3 replications each. When seeds
were germinating, irradiation treatment was applied according to the light / dark period as given (Table 1).
UV-B radiation was generated by a filtered lamp (30 W, 297 nm, Qin brand, Baoji Lamp Factory, Baoji City, China.). The
lamps were hung above the plants 40cm, the irradiation was obtained by adjusting the distance between the lamps and the
pots. Daily dose were 0 and 10.08 kJ m-2 UV-B, respectively.
2. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-2, February- 2016]
Page | 150
TABLE 1
LIGHT / DARK TREATMENT
Treatment
UV-B irritation (daily dose) Dark
0 10.08 kJ·m-2
CK1 + - 16 hr·d-1
B1 - + 16 hr·d-1
CK2 + - 16 hr·d-1
B2 - + 16 hr·d-1
CK3 + - 16 hr·d-1
B3 - + 16 hr·d-1
CK1-3 soybean cultivars without UV-B radiation; B1-3: soybean cultivars with UV-B radiation
2.2 Measurement of plant height
Plant height and internode length were measured twice weekly, and three seedlings per replication were randomly selected
from each treatment for the downstream measurements.
2.3 Soluble sugars analysis
Soluble carbohydrates were determined according to the anthrone method (14). One ml of leaf extraction was added to 3 ml
(final volume) solution containing 1.08 M H2SO4, 1.09 mM thiourea and 2.1 mM anthrone. The mixture was heated at
100C for 10 min and the absorbance at 620 nm was recorded. A calibration curve with glucose was a standard.
2.4 Assay of free prolines
Proline content was assayed followed the method described by Bates (15). Briefly, extraction from leaf was mixed with acid-
ninhydrin and glacial acetic acid, and then heated at 100C in a water bath for 10 min, the reaction was terminated on ice
followed by toluene added in. The proline-ninhydrin chromophore in the upper phase was used to measure absorbance based
on dry weight.
2.5 Determination of total protein content
Total protein content was quantified according to Bradford (16) where BSA (bovine serum albumin) was used as the
standard.
2.6 Contents of flavonoids and relative anthocyanins assay
Leaves kept at 55 were frozen with liquid N2 and ground to fine powder then suspended with 60% aqueous alcohol. The
supernatant was used to detect flavonoids. Briefly, 5% NaNO2 was added then the mixture was incubated, followed by
adding Al(NO3)3 and NaOH. Absorbance was recorded and flavonoids were calculated on dry weight base. The relative
anthocyanins content was assayed according to Zhang (17).
2.7 Analysis of SOD, APX, GR activity and ascorbic acid,GSH level
Superoxide Dismutase (SOD) activity was determined based on the photochemical reduction of nitroblue tetrazolium (NBT).
One unit of SOD activity is defined as the amount of enzyme that inhibite the NBT reduction by 50%. Ascorbate peroxidase
(APX) activity was measured according to Nakano and Asada (18).Glutathione reductase (GR) activity and Glutathione
(GSH) content were measured according to Ban Wang et al. (19). Ascorbic acid (ASA) content was determined by
Mukherjee and Choudhouri (20).
2.8 Statistical analysis
Statistical significance is estimated at P < 0.05 followed Duncan‟s multiple range test. All data were given means ± SD.
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III. RESULTS
3.1 Effects of UV-B irradiance on seedling height and node length of soybean
TABLE 2
RESPONSES OF PLANT HEIGHT AND NODE LENGTH UNDER UV-B RADIATION
Varieties
Length of nodes (cm) Plant height
cm1st 2nd 3rd 4th
CK1 10.7±0.41a 8.6±0.52 a 11.2±0.67a 6.6±0.56a 44.5±0.53a
B1 9.5±0.63b 6.7±0.46d 7.9±0.35c 4.5±0.23c 34.3±0.48b
CK2 10.1±0.78a 7.8±0.93c 8.5±0.82b 5.3±0.23b 36.8±0.84b
B2 9.3±0.81b 6.9±0.73d 6.4±0.58d 3.5±0.20d 29.5±0.70c
CK3 9.5±1.12b 8±0.85b 7.7±0.95c 4.0±0.26c 33.8±0.97b
B3 8.5±0.63c 7±0.54d 4.9±0.69d 0.8±0.05e 28.8±0.62c
Different letters indicate significant differences between treatments at 0.05 level according to Duncan’s multiple range
test (n=4), the same below. CK1: Dongnong 42 without UV-B, CK2: Zhonghuang 57 without UV-B, CK3: Jin 36
without UV-B, B1: Dongnong 42 with UV-B; B2: Zhonghuang 57 with UV-B, B3: Jin 36 with UV-B.
UV-B treatment significantly suppressed plant height where they were 22.92%, 19.84% and 14.79% lower in Dongnong 42,
Zhonghuang 57 and Jin 36 than the control (p<0.05). The lowest and the highest seedling height were observed in Jin 36 and
Dongnong 42, respectively.
The dwarf induced by UV-B radiation presented from the first node for each variety. For the first node the reduction level
was Dongnong 42>Jin 36>Zhonghuang 57. The second node was much dwarfed than the first one where they were
decreased by 22.09% in Dongnong 42 group, 24.71% in Zhonghuang 57 and12.50% in Jin 36. Considerable decreasing was
found in the third node where the shortest node of 2.5cm showed in Jin 36. In the fourth node, maximum inhibition showed
where it was 0.8 cm in Jin 36 group (p<0.05)(Table 2).
3.2 Effects of UV-B irradiance on contents of flavonoids, anthocyanins and protein
FIG.1 FLAVONOIDS, ANTHOCYANINS AND PROTEIN CONTENT UNDER UV-B RADIATION
Different letters indicate significant differences between treatments at 0.05 level according to Duncan’s multiple range
test (n=4). D: Dongnong 42; Z: Zhonghuang 57; J: Jin 36
The content of flavonoids in different soybean cultivars varied where Jin 36 >Dongnong 42 > Zhonghuang 57, but it
increased in all groups when exposed to UV-B. The maximum content was observed in Dongnong 42 (1.05mg/g), followed
by Jin 36 (0.64mg/g) and Zhonghuang 57 (0.46mg/g). It raised by 325%, 165% and 61% in Dongnong42, Zhonghuang 57
and Jin 36 groups, respectively (p<0.05).
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The relative content of anthocyanins was significantly increased when exposed to UV-B radiation. It improved 400%, 325%
and 80% in Dongnong 42, Zhonghuang 57 and Jin 36, (p<0.05) respectively. The highest content was showed in Dongnong
42, and it was the lowest in Zhonghuang 57.
After 25 day (from germination) of UV-B irradiation, protein content was significant increased (p<0.05) where they
increased 50.36%, 20.27% and 13.29% in Dongnong 42, Zhonghuang 57 and Jin 36, respectively (p<0.05). Maximum
protein content was found in Zhonghuang 57 whenever with or without UV-B radiation (Fig.1).
3.3 Effects of UV-B irradiation on contents of proline and soluble sugar in various soybean cultivars
FIG. 2 UV-B AFFECTS CONTENTS OF PROLINE AND SOLUBLE SUGAR
Different letters indicate significant differences between treatments at 0.05 level according to Duncan’s multiple range
test (n=4) D: Dongnong 42; Z: Zhonghuang 57; J: Jin 36
Soluble sugar decreased under UV-B radiation and it was not the case for proline. For Dongnong 42, soluble sugar decreased
by 45.09% and proline increased by 17.18% (p<0.05), they were 7.87% lower (p>0.05) and 14.25% higher (p<0.05) in
Zhonghuang 57; 25.14% lower and 32.85% higher in Jin 36 (p<0.05), respectively. The highest proline level and sugar both
appeared in Zhonghuang 57(Fig.2).
3.4 Effects of UV-B irradiation on antioxidative enzyme activities of SOD、GR and APX
After 25 days of UV-B radiation, activities of antioxidative enzyme as SOD, GR and APX in the three cultivars considerably
increased where they increased 45.22%, 62.49% and 96.91% in Dongnong 42 (p<0.05); 39.10%,65.82%,55.44% in
Zhonghuang 57 (p<0.05), 32.39%, 66.02%,48.54% in Jin 36(p<0.05). The highest SOD, GR and APX activity turn up in
Zhonghuang 57 group, and the lowest SOD GR and APX activities occured in Dongnong 42(Fig.3).
FIG.3 ANTIOXIDATIVE ENZYME ACTIVITIES OF SOD、GR AND APX EXPOSED TO UV-B.
Different letters indicate significant differences between treatments at 0.05 level according to Duncan’s multiple range
test (n=4) D: Dongnong 42; Z: Zhonghuang 57; J: Jin 36
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3.5 Effects of UV-B irradiation on GSH and ASA
Significantly increased GSH and ASA were observed in three soybean cultivars under UV-B irradiation where they increased
252% and 83% in Dongnong 42; 145% and 78% in Zhonghuang 57; 68% and 31% in Jin 36, respectively, (p<0.05). The
highest GSH and ASA presented in Dongnong 42, followed by Zhonghuang 57and Jin 36 (p<0.05) (Fig.4).
FIG.4 CONTENTS OF GSH AND ASA EXPOSED TO UV-B
Different letters indicate significant differences between treatments at 0.05 level according to Duncan’s multiple range
test (n=4). D: Dongnong 42; Z: Zhonghuang 57; J: Jin 36
IV. DISCUSSION
Plant height is an important agronomic trait. Reduction in plant height upon to enhanced UV-B has been reported in several
crops (21). Tevini et al. (22) found that indoleacetic acid, a growth hormone, was absorbed in the UV-B range and believed
that UV-B dependent photooxidation might be the reason for the dwarfed shoot. The present study showed UV-B
significantly inhibited the plant height of the three soybean cultivars, especially Dongnong42. Given that nodes were
fundamental part of plant height, the inhibitory occurred in nodes was easy to understand. UV-B seriously inhibited the
fourth node of Jin 36. The first three nodes were more dwarfed in Dongnong 42 than the other cultivars. Besides, the stems
exposed to UV-B irradiation became thin and weak, especially in Zhonghong 57 which might due to the varied substances in
stems and the altered allocation of photosynthate (23).
Plant leaves are the target of solar radiation. Pal et al. indicated leaf orientation might be an important factor accounts for the
sensitivity under UV-B (24). He illustrated that maize was less sensitive to UV-B might due to near-vertical leave than mung
bean whose leaves are horizontal in orientation. Decreased leaf area under supplemental UV-B has been reported for a
number of dicotyledonous species including cucumber, radish, soybean and garden bean (25-27). Murali et al. (3) manifested
that leaf thickness was related to UV-B sensitivity, and he believed thicker leaves could mitigate UV-B radiation through
additional tissue absorbance, thereby prevent a greater proportion of UV from reaching sensitive organelles in mesophyll
tissues. We observed the leaves of three varieties soybean became thicker and smaller after UV-B radiation, which agreed
with the above reports.
Besides leaf, UV-B sensitivity was also correlated with the change of certain biochemical substances in leaves. Most plants
produce more flavonoids upon UV-B radiation (27), though the absolute amounts of constitutive and UV-B-Induced
flavonoids varied greatly from species to species. Flavonoids accumulation occurs under both low and high UV-B radiation.
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Couple of reports argued that the function of phenylpropanoids and flavonoids that accumulated via UV-B-mediated pathway
is to increase ROS scavenging activity (5, 28). It was reported the UV-Induced improved quercetin: kaempferolrol ratio
represented an increase in ROS scavenging activity, rather than an increase in UV absorbance (29) One of flavonoids, the
anthocyanins (ANT), which is highly water soluble pigment and accumulates in vacuole (30), was considered beneficial to
plants for alleviating damage to photosynthetic systems via UV-B absorption (31). It was believed the significantly ANT
accumulation upon heat stress might account for the increased osmotic potential of leaves, so as to increase the water
absorption and reduce transpirational losses (32). Based on this, we speculated the flavonoids accumulated upon UV-B
radiation served for the absorption of UV-B, ROS scavenge, also the osmotic control. Lately study indicated that changes in
ROS and antioxidant metabolism were intrinsic response to UV-B radiation. It was reported high level of UV-B might
elevate ROS level or membrane-localized NADPH-oxidase, and genes expression which may be controlled through UVR8,
COP1 and HY5 (2) Besides, UVR8 improved the expression of chloroplastic proteins such as SIG5 and ELIP, which might
protect photosynthesis by regulating the secondary metabolites and photomorphogenesis (33).
At the present study, significantly improved contents of flavonoids, anthocyanins, GSH, ASA as well as increased APX, NR,
SOD activities were observed in the three soybean cultivars when exposed to UV-B. Slightly higher SOD, GR and APX
activities in Zhonghuang 57 was detected compared with those in Jin36 which might be related to higher protein content.
However, higher levels GSH, ASA, flavonoids and anthocyanins in Dongnong 42 were observed than other cultivars after
UV-B radiation.
Chen and Murata (36) presented that proline, as a molecular chaperone can modify allosteric enzyme in adversity and
protected the electron transfer of mitochondrial complex II. Park et al. (37) indicated proline can effectively protect and
repair PSII damage in adversity. Smirnoff and Cumbes (38) suggested proline could act as active oxygen scavenger. We
speculated the increased proline in three soybean cultivars might be the acclimation to UV-B radiation. Photosynthesis
converts light energy into the chemical of sugar and organic compounds, therefore the levels of sugar in plant was closely
related to photosynthesis. Approximately 80% of the CO2 assimilated during photosynthesis was soluble sugar, which is the
major transport form of organic carbon exported from the source to sink (39). The change of soluble sugar induced by stress
can lead to loss in agricultural production (40). Thereby we deem the reduction of soluble sugar in three soybean cultivars
under the UV-B indicated a greater movement of assimilates from the source to sink which is beneficial to yield formation.
The results are consistent with Ana et al (41).
V. CONCLUSION
Various soybean cultivars showed varied physiological responses upon to UV-B, but the trend is consistent. The result
further showed that enzymatic and non-enzymatic antioxidants SOD, APX, GR, ASA, GSH, increased in leaf of the three
cultivars which developed a strong network defense system through UV-B radiation. Both flavonoids and protein contents
significantly increased which may be related to the increased secondary metabolism of plants. The improvement of osmotic
regulation substances as proline and anthocyanins may be a adjustment of plant to UV-B. The soluble sugar decreased among
the three cultivars which suggested UV-B affect the photosynthesis, not negatively.
ACKNOWLEDGEMENTS
This research project is supported by the Fund Program for the Scientific Activities of Selected Returned Overseas
Professionals in Shanxi Province.
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