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ISSN No: 2456
International
Research
Green Synthesis of ZnO Nanoparticles using
Leaf Extract and its Spectral and Optical Characterization Studies
Assistant Professor, Department of Physics,
S.D.N.B. Vaishnav College for Women,
ABSTRACT
The main objective of the present study
the green synthesis of Zinc Oxide [ZnO] nanoparticles
utilizing aqueous leaf extract of
Fluminense.` Zinc acetate [Zn(O2CCH3
sodium hydroxide [NaOH] were used as starting
materials and Jasminum Fluminense
leaf extract is used as precursor in the synthesis of
ZnO nanoparticles. The resultant nanopowder was
characterized by using Ultraviolet [UV]
Spectroscopy, Fourier Transform Infrared
Spectroscopy [FTIR] and Transmission Electron
Microscopic [TEM] studies. Formation of ZnO
nanoparticles has been confirmed by UV
spectroscopy and the TEM analysis spectacles tha
synthesized ZnO nanoparticles are of face centered
cubic (fcc) structure and the size is found to be around
20 nm. FTIR spectral analysis indicated the leaf
extract acts as the reducing and capping agents on the
surface of ZnO nanoparticles. This sim
green approach may provide a useful tool to hefty
extent in the synthesis of ZnO nanoparticles.
synthesized nanostructures illustrate novel
applications in many fields such as cosmetics,
optoelectronics, sensors, transducers and biomedica
science because it is environmentally friendly and
does not involve any harmful substances
Keywords: Leaf extract, Nanopowder,
Fluminense and Biomedical science
1. INTRODUCTION
In recent years the growth of hazardless free metal
nanoparticles has become a great challenge. Different
metal nanoparticles can be synthesized but among
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 4 | May-Jun
ISSN No: 2456 - 6470 | www.ijtsrd.com | Volume
International Journal of Trend in Scientific
Research and Development (IJTSRD)
International Open Access Journal
Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense
Leaf Extract and its Spectral and Optical Characterization Studies
Dr. S. Amudha
Assistant Professor, Department of Physics,
S.D.N.B. Vaishnav College for Women, Chennai, Tamil Nadu, India
present study is to deal with
xide [ZnO] nanoparticles
lizing aqueous leaf extract of Jasminum
3)2(H2O)2] and
used as starting
[Nithyamalli]
in the synthesis of
ZnO nanoparticles. The resultant nanopowder was
Ultraviolet [UV] – Visible
Transform Infrared
IR] and Transmission Electron
Formation of ZnO
nanoparticles has been confirmed by UV-visible
spectroscopy and the TEM analysis spectacles that the
icles are of face centered
structure and the size is found to be around
indicated the leaf
as the reducing and capping agents on the
This simplistic and
green approach may provide a useful tool to hefty
synthesis of ZnO nanoparticles. These
synthesized nanostructures illustrate novel
applications in many fields such as cosmetics,
and biomedical
science because it is environmentally friendly and
does not involve any harmful substances.
Nanopowder, Jasminum
In recent years the growth of hazardless free metal
nanoparticles has become a great challenge. Different
metal nanoparticles can be synthesized but among
them Zinc Oxide (ZnO) plays a vital role in chemical
industries due to its anti-corrosive and anti
activities. Metal nanoparticles can be prepared by
both physical and chemical methods such as UV
irradiation, microwave irradiation, chemical
reduction, photochemical method, electron irradiation
and sonoelectrochemical method [1]. But these
methods are costly, labor intensive and hazardous to
environment as well as living organisms and in order
to eliminate this we need an alternative,
environmentally friendly, cost
approach [2]. So green approach of nanoparticle
synthesis has gained great attention among scientists
and literature review divulges that leaves are
generally used for the synthesis of metal nanoparticles
because they act as both reducing and stabilizing
agent.
Generally nanoparticles are the cluster of atoms in
nanometer range. There are two types of nanoparticles
namely metal and metal oxide nanoparticle
them are very important but metal oxide nanoparticle
such as CuO, TiO2 and ZnO have semi
properties. Now-a-days ZnO nanoparticle has
great attention among researchers owing to its
different characteristics such as
conductivity and cytotoxicity [3
synthesis of ZnO nanoparticles from leaves such as
Moringa oleifera, Lemon grass, C
and Catharanthus roseus has already been reported
[4-8]. In our point of view, ZnO nanoparticles from
Jasminum Fluminense using Zinc acetate and Sodium
hydroxide as starting material
first time. Commonly, ZnO is a metal oxide
semiconductor belonging to II
Jun 2018 Page: 258
www.ijtsrd.com | Volume - 2 | Issue – 4
Scientific
(IJTSRD)
International Open Access Journal
Jasminum Fluminense
Leaf Extract and its Spectral and Optical Characterization Studies
Tamil Nadu, India
them Zinc Oxide (ZnO) plays a vital role in chemical
corrosive and anti-bacterial
activities. Metal nanoparticles can be prepared by
both physical and chemical methods such as UV
irradiation, microwave irradiation, chemical
reduction, photochemical method, electron irradiation
and sonoelectrochemical method [1]. But these
s are costly, labor intensive and hazardous to
environment as well as living organisms and in order
to eliminate this we need an alternative,
environmentally friendly, cost-effective and safest
approach [2]. So green approach of nanoparticle
gained great attention among scientists
and literature review divulges that leaves are
generally used for the synthesis of metal nanoparticles
because they act as both reducing and stabilizing
Generally nanoparticles are the cluster of atoms in
nanometer range. There are two types of nanoparticles
tal oxide nanoparticles. Both of
ut metal oxide nanoparticle
ZnO have semiconductor
days ZnO nanoparticle has gained
great attention among researchers owing to its
uch as catalysis, electrical
conductivity and cytotoxicity [3]. The biological
synthesis of ZnO nanoparticles from leaves such as
Lemon grass, Coriandrum sativum
has already been reported
ZnO nanoparticles from
using Zinc acetate and Sodium
as starting material was reported for the
ZnO is a metal oxide
semiconductor belonging to II-VI group having large
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 4 | May-Jun 2018 Page: 259
exciton binding energy of 60 meV and wide band gap
of about 3.4 eV. It has wide application in solar cells,
gas sensors, ceramics, catalysts and used as an
additive in paints, cosmetics, plastics, rubber
manufacturing, electronics, pharmaceuticals,
agriculture and aquaculture [9]. The plant Jasminum
Fluminense belonging to the Olive family called
Oleaceae and it contains about 200 species native to
tropical and warm temperate regions of Eurasia,
Australasia and Oceania. It is a climber plant
belonging to dicotyledon and angiosperm group. The
main aim of the present study is to synthesize Zinc
Oxide (ZnO) nanoparticles using aqueous leaf extract
of Jasminum Fluminense and to evaluate the structure
by various characterization tools such as Ultraviolet
[UV] – Visible Spectroscopy, Fourier Transform
Infrared Spectroscopy [FTIR] and Transmission
Electron Microscopic [TEM] studies.
2. MATERIALS AND METHODS
2.1 Materials Required
Zinc acetate [Zn (O2CCH3)2(H2O)2], Sodium
hydroxide [NaOH] pellet and glasswares were
purchased from Merck and used as received and
Jasminum Fluminense leaves were collected from
SDNB Vaishnav College Campus. The leaves and all
the glasswares were thoroughly washed with double
distilled water before use.
2.2 Preparation of Jasminum
Fluminense Leaf Extract
Fresh leaves of Jasminum Fluminense about 10 g
were collected and washed several times with tap
water and then with distilled water and cut into small
pieces. These leaves were boiled with 100 ml of
double distilled water at 60 C for about 30 minutes.
After boiling, color of the solution changes to light
brown color and it was cooled at room temperature.
This extract was filtered through Whatman Number-1
filter paper and stored in refrigerator for further
characterization studies.
2.3 Green Synthesis of ZnO
Nanoparticles
For the synthesis of ZnO nanoparticles 1mM Zinc
acetate [Zn (O2CCH3)2 (H2O) 2] was dissolved in 50
ml of distilled water and kept in stirrer for 1 hr
respectively. Then 1mM of Sodium hydroxide
[NaOH] Pellets was dissolved in 20 ml of distilled
water and kept in stirrer for 1 hr respectively. Then 25
ml of Jasminum Fluminense leaf extract was added
drop wise to the above mixture and continuously
stirred at 50 C for another 1 hr until the colloidal
solution is obtained. The colour of the resultant
solution changes to light pale yellow colour which
confirms the presence of ZnO nanoparticles. The
precipitate was centrifuged at 10000 rpm at 50 °C for
20 min and powdered specimen was collected. This
yellow coloured sample was dried using a hot air oven
operating at 70 °C for 2 h and crushed using ceramic
mortar and pestle to get fine Zinc Oxide (ZnO)
nanoparticles and stored in air-tight bottles for further
characterization studies [10].
2.4 Characterization
The optical properties of synthesized ZnO
nanoparticles were investigated using UV-visible
8500 spectrophotometer in the wavelength range 250-
1000 nm. The FTIR spectra were recorded using KBr
pellet method by FTIR Spectrophotometer (Bruker,
Tensor 27) at the wave number resolution of 1 cm-1
and with a total number of scans as 32 in the range
4000-650 cm-1
on the transmittance mode. The
morphology was observed by Transmission Electron
Microscopy using Hitachi Model TEM.
3. RESULTS AND DISCUSSION
3.1 UV-visible Spectroscopic Results
Fig. 1 UV-visible spectrum of biosynthesized ZnO
nanoparticles
Figure 1 shows the UV-visible absorption spectrum
obtained for the synthesized Zinc Oxide (ZnO)
nanoparticles recorded between the range 250-1000
nm. It is known that the ZnO nanoparticles have free
electrons due to which Surface Plasmon Resonance
(SPR) absorption band appear at 289.9 nm. This
absorption peak indicates the reduction of Zn2+
ions in
the reaction medium which authenticates the
formation of ZnO nanoparticles [4, 11]. No other
peaks have been observed which confirms the
200 300 400 500 600 700 800 900 1000
0.0
0.1
0.2
0.3
0.4
0.5
Absorbance(A)
Wavelength(nm)
289.9nm ZnOnanoparticles
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 4 | May-Jun 2018 Page: 260
presence of ZnO nanoparticles only. From the UV-
visible graph the energy band gap is calculated using
the formula
The energy band gap value is found to be 4.28 eV
which is more than pure ZnO nanoparticles i.e., 3.4
eV.
3.2 FTIR Spectral Analysis of ZnO Nanoparticles
FTIR spectral analysis was carried out to find the
functional groups present in ZnO nanoparticles and
the FTIR spectra of ZnO nanoparticles is shown in
Figure 2. The spectrum portraying band at 2924 and
2856 cm-1
are due to C-H stretching vibration [12].
The band at 1750 cm-1
corresponds to C=O stretching
[9] whereas the peak at 1561 cm-1
is attributed to
asymmetric stretching band from –COO- groups of
acetate ions respectively [13]. The intense vibrational
band around 1401 cm-1
is assigned to symmetric
stretching of carbonyl side group [14] whereas the
band at 922 and 858 cm-1
may correspond to O-H
bending vibrations of carboxylic acid [15] and C-H
bending vibration [10]. The vibrational band observed
at 708 and 666 cm-1
corresponds to –C=C-H [16] and
M-O stretching of ZnO [17] nanoparticles. The FTIR
spectra thus demonstrates the structural changes
taking place in the ZnO nanoparticles and Jasminum
Fluminense leaf extract by the co-ordination of O-H,
C-H, C=O and –COO- bonds.
Fig. 2 FTIR spectra obtained for biosynthesized ZnO nanoparticles
3.3 TEM Results
Fig. 3 TEM image of synthesized ZnO nanoparticles at different magnifications
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 4 | May-Jun 2018 Page: 261
The morphology and size of Zinc Oxide (ZnO)
nanoparticles examined using Transmission Electron
Microscopic (TEM) analysis as shown in Figure 3.
These images depicts that the nanoparticles are
hexagonal, rod shaped and some of them are spherical
at different magnifications. These spherical structures
indicate the presence of amorphous nature of ZnO
nanoparticles [2]. The average diameter of the ZnO
nanoparticle is found to be 20 nm which is correlated
with the earlier reports [18].
CONCLUSIONS
In this present study eco-friendly, easy synthesis, low-
cost, non-hazardous, organically effective and
innovative approach of the biosynthesized ZnO
nanoparticles using Jasminum Fluminense leaf extract
have been reported. The phytochemicals present in the
leaf extract acts as a biological stabilizing and
reducing agent for the synthesis of metal oxide
nanoparticles. The presence of ZnO nanoparticles was
confirmed using UV-visible, FTIR and TEM studies.
The absorption band observed at 289.9 nm with an
energy band gap of 4.28 eV is confirmed by UV-
visible spectroscopic studies. FTIR result confirms the
presence of functional groups in ZnO nanoparticles.
The particle size was found to be around 20 nm with
hexagonal Wurtzite structure as evidenced from TEM
results. Thus, it is exemplified that the ZnO
nanoparticles synthesized in this research work acts as
a promising candidate for future biological
applications.
REFERENCES
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(2017), 249-252.
8) G. Bhumi, N. Savithramma, “Biological synthesis
of zinc oxide nanoparticles from
Catharanthusroseus G. Don. Leaf extract and
validation for antibacterial activity”, Int. J. Drug
Dev. & Res., Vol. 6, (2014), 208-214.
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nanoparticles synthesized using L. aculeata leaf
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fungal pathogens”, Bull. Mater. Sci., Vol. 39,
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Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract and its Spectral and Optical Characterization Studies

  • 1. @ IJTSRD | Available Online @ www.ijtsrd.com ISSN No: 2456 International Research Green Synthesis of ZnO Nanoparticles using Leaf Extract and its Spectral and Optical Characterization Studies Assistant Professor, Department of Physics, S.D.N.B. Vaishnav College for Women, ABSTRACT The main objective of the present study the green synthesis of Zinc Oxide [ZnO] nanoparticles utilizing aqueous leaf extract of Fluminense.` Zinc acetate [Zn(O2CCH3 sodium hydroxide [NaOH] were used as starting materials and Jasminum Fluminense leaf extract is used as precursor in the synthesis of ZnO nanoparticles. The resultant nanopowder was characterized by using Ultraviolet [UV] Spectroscopy, Fourier Transform Infrared Spectroscopy [FTIR] and Transmission Electron Microscopic [TEM] studies. Formation of ZnO nanoparticles has been confirmed by UV spectroscopy and the TEM analysis spectacles tha synthesized ZnO nanoparticles are of face centered cubic (fcc) structure and the size is found to be around 20 nm. FTIR spectral analysis indicated the leaf extract acts as the reducing and capping agents on the surface of ZnO nanoparticles. This sim green approach may provide a useful tool to hefty extent in the synthesis of ZnO nanoparticles. synthesized nanostructures illustrate novel applications in many fields such as cosmetics, optoelectronics, sensors, transducers and biomedica science because it is environmentally friendly and does not involve any harmful substances Keywords: Leaf extract, Nanopowder, Fluminense and Biomedical science 1. INTRODUCTION In recent years the growth of hazardless free metal nanoparticles has become a great challenge. Different metal nanoparticles can be synthesized but among @ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 4 | May-Jun ISSN No: 2456 - 6470 | www.ijtsrd.com | Volume International Journal of Trend in Scientific Research and Development (IJTSRD) International Open Access Journal Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract and its Spectral and Optical Characterization Studies Dr. S. Amudha Assistant Professor, Department of Physics, S.D.N.B. Vaishnav College for Women, Chennai, Tamil Nadu, India present study is to deal with xide [ZnO] nanoparticles lizing aqueous leaf extract of Jasminum 3)2(H2O)2] and used as starting [Nithyamalli] in the synthesis of ZnO nanoparticles. The resultant nanopowder was Ultraviolet [UV] – Visible Transform Infrared IR] and Transmission Electron Formation of ZnO nanoparticles has been confirmed by UV-visible spectroscopy and the TEM analysis spectacles that the icles are of face centered structure and the size is found to be around indicated the leaf as the reducing and capping agents on the This simplistic and green approach may provide a useful tool to hefty synthesis of ZnO nanoparticles. These synthesized nanostructures illustrate novel applications in many fields such as cosmetics, and biomedical science because it is environmentally friendly and does not involve any harmful substances. Nanopowder, Jasminum In recent years the growth of hazardless free metal nanoparticles has become a great challenge. Different metal nanoparticles can be synthesized but among them Zinc Oxide (ZnO) plays a vital role in chemical industries due to its anti-corrosive and anti activities. Metal nanoparticles can be prepared by both physical and chemical methods such as UV irradiation, microwave irradiation, chemical reduction, photochemical method, electron irradiation and sonoelectrochemical method [1]. But these methods are costly, labor intensive and hazardous to environment as well as living organisms and in order to eliminate this we need an alternative, environmentally friendly, cost approach [2]. So green approach of nanoparticle synthesis has gained great attention among scientists and literature review divulges that leaves are generally used for the synthesis of metal nanoparticles because they act as both reducing and stabilizing agent. Generally nanoparticles are the cluster of atoms in nanometer range. There are two types of nanoparticles namely metal and metal oxide nanoparticle them are very important but metal oxide nanoparticle such as CuO, TiO2 and ZnO have semi properties. Now-a-days ZnO nanoparticle has great attention among researchers owing to its different characteristics such as conductivity and cytotoxicity [3 synthesis of ZnO nanoparticles from leaves such as Moringa oleifera, Lemon grass, C and Catharanthus roseus has already been reported [4-8]. In our point of view, ZnO nanoparticles from Jasminum Fluminense using Zinc acetate and Sodium hydroxide as starting material first time. Commonly, ZnO is a metal oxide semiconductor belonging to II Jun 2018 Page: 258 www.ijtsrd.com | Volume - 2 | Issue – 4 Scientific (IJTSRD) International Open Access Journal Jasminum Fluminense Leaf Extract and its Spectral and Optical Characterization Studies Tamil Nadu, India them Zinc Oxide (ZnO) plays a vital role in chemical corrosive and anti-bacterial activities. Metal nanoparticles can be prepared by both physical and chemical methods such as UV irradiation, microwave irradiation, chemical reduction, photochemical method, electron irradiation and sonoelectrochemical method [1]. But these s are costly, labor intensive and hazardous to environment as well as living organisms and in order to eliminate this we need an alternative, environmentally friendly, cost-effective and safest approach [2]. So green approach of nanoparticle gained great attention among scientists and literature review divulges that leaves are generally used for the synthesis of metal nanoparticles because they act as both reducing and stabilizing Generally nanoparticles are the cluster of atoms in nanometer range. There are two types of nanoparticles tal oxide nanoparticles. Both of ut metal oxide nanoparticle ZnO have semiconductor days ZnO nanoparticle has gained great attention among researchers owing to its uch as catalysis, electrical conductivity and cytotoxicity [3]. The biological synthesis of ZnO nanoparticles from leaves such as Lemon grass, Coriandrum sativum has already been reported ZnO nanoparticles from using Zinc acetate and Sodium as starting material was reported for the ZnO is a metal oxide semiconductor belonging to II-VI group having large
  • 2. International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470 @ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 4 | May-Jun 2018 Page: 259 exciton binding energy of 60 meV and wide band gap of about 3.4 eV. It has wide application in solar cells, gas sensors, ceramics, catalysts and used as an additive in paints, cosmetics, plastics, rubber manufacturing, electronics, pharmaceuticals, agriculture and aquaculture [9]. The plant Jasminum Fluminense belonging to the Olive family called Oleaceae and it contains about 200 species native to tropical and warm temperate regions of Eurasia, Australasia and Oceania. It is a climber plant belonging to dicotyledon and angiosperm group. The main aim of the present study is to synthesize Zinc Oxide (ZnO) nanoparticles using aqueous leaf extract of Jasminum Fluminense and to evaluate the structure by various characterization tools such as Ultraviolet [UV] – Visible Spectroscopy, Fourier Transform Infrared Spectroscopy [FTIR] and Transmission Electron Microscopic [TEM] studies. 2. MATERIALS AND METHODS 2.1 Materials Required Zinc acetate [Zn (O2CCH3)2(H2O)2], Sodium hydroxide [NaOH] pellet and glasswares were purchased from Merck and used as received and Jasminum Fluminense leaves were collected from SDNB Vaishnav College Campus. The leaves and all the glasswares were thoroughly washed with double distilled water before use. 2.2 Preparation of Jasminum Fluminense Leaf Extract Fresh leaves of Jasminum Fluminense about 10 g were collected and washed several times with tap water and then with distilled water and cut into small pieces. These leaves were boiled with 100 ml of double distilled water at 60 C for about 30 minutes. After boiling, color of the solution changes to light brown color and it was cooled at room temperature. This extract was filtered through Whatman Number-1 filter paper and stored in refrigerator for further characterization studies. 2.3 Green Synthesis of ZnO Nanoparticles For the synthesis of ZnO nanoparticles 1mM Zinc acetate [Zn (O2CCH3)2 (H2O) 2] was dissolved in 50 ml of distilled water and kept in stirrer for 1 hr respectively. Then 1mM of Sodium hydroxide [NaOH] Pellets was dissolved in 20 ml of distilled water and kept in stirrer for 1 hr respectively. Then 25 ml of Jasminum Fluminense leaf extract was added drop wise to the above mixture and continuously stirred at 50 C for another 1 hr until the colloidal solution is obtained. The colour of the resultant solution changes to light pale yellow colour which confirms the presence of ZnO nanoparticles. The precipitate was centrifuged at 10000 rpm at 50 °C for 20 min and powdered specimen was collected. This yellow coloured sample was dried using a hot air oven operating at 70 °C for 2 h and crushed using ceramic mortar and pestle to get fine Zinc Oxide (ZnO) nanoparticles and stored in air-tight bottles for further characterization studies [10]. 2.4 Characterization The optical properties of synthesized ZnO nanoparticles were investigated using UV-visible 8500 spectrophotometer in the wavelength range 250- 1000 nm. The FTIR spectra were recorded using KBr pellet method by FTIR Spectrophotometer (Bruker, Tensor 27) at the wave number resolution of 1 cm-1 and with a total number of scans as 32 in the range 4000-650 cm-1 on the transmittance mode. The morphology was observed by Transmission Electron Microscopy using Hitachi Model TEM. 3. RESULTS AND DISCUSSION 3.1 UV-visible Spectroscopic Results Fig. 1 UV-visible spectrum of biosynthesized ZnO nanoparticles Figure 1 shows the UV-visible absorption spectrum obtained for the synthesized Zinc Oxide (ZnO) nanoparticles recorded between the range 250-1000 nm. It is known that the ZnO nanoparticles have free electrons due to which Surface Plasmon Resonance (SPR) absorption band appear at 289.9 nm. This absorption peak indicates the reduction of Zn2+ ions in the reaction medium which authenticates the formation of ZnO nanoparticles [4, 11]. No other peaks have been observed which confirms the 200 300 400 500 600 700 800 900 1000 0.0 0.1 0.2 0.3 0.4 0.5 Absorbance(A) Wavelength(nm) 289.9nm ZnOnanoparticles
  • 3. International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470 @ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 4 | May-Jun 2018 Page: 260 presence of ZnO nanoparticles only. From the UV- visible graph the energy band gap is calculated using the formula The energy band gap value is found to be 4.28 eV which is more than pure ZnO nanoparticles i.e., 3.4 eV. 3.2 FTIR Spectral Analysis of ZnO Nanoparticles FTIR spectral analysis was carried out to find the functional groups present in ZnO nanoparticles and the FTIR spectra of ZnO nanoparticles is shown in Figure 2. The spectrum portraying band at 2924 and 2856 cm-1 are due to C-H stretching vibration [12]. The band at 1750 cm-1 corresponds to C=O stretching [9] whereas the peak at 1561 cm-1 is attributed to asymmetric stretching band from –COO- groups of acetate ions respectively [13]. The intense vibrational band around 1401 cm-1 is assigned to symmetric stretching of carbonyl side group [14] whereas the band at 922 and 858 cm-1 may correspond to O-H bending vibrations of carboxylic acid [15] and C-H bending vibration [10]. The vibrational band observed at 708 and 666 cm-1 corresponds to –C=C-H [16] and M-O stretching of ZnO [17] nanoparticles. The FTIR spectra thus demonstrates the structural changes taking place in the ZnO nanoparticles and Jasminum Fluminense leaf extract by the co-ordination of O-H, C-H, C=O and –COO- bonds. Fig. 2 FTIR spectra obtained for biosynthesized ZnO nanoparticles 3.3 TEM Results Fig. 3 TEM image of synthesized ZnO nanoparticles at different magnifications
  • 4. International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470 @ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 4 | May-Jun 2018 Page: 261 The morphology and size of Zinc Oxide (ZnO) nanoparticles examined using Transmission Electron Microscopic (TEM) analysis as shown in Figure 3. These images depicts that the nanoparticles are hexagonal, rod shaped and some of them are spherical at different magnifications. These spherical structures indicate the presence of amorphous nature of ZnO nanoparticles [2]. The average diameter of the ZnO nanoparticle is found to be 20 nm which is correlated with the earlier reports [18]. CONCLUSIONS In this present study eco-friendly, easy synthesis, low- cost, non-hazardous, organically effective and innovative approach of the biosynthesized ZnO nanoparticles using Jasminum Fluminense leaf extract have been reported. The phytochemicals present in the leaf extract acts as a biological stabilizing and reducing agent for the synthesis of metal oxide nanoparticles. The presence of ZnO nanoparticles was confirmed using UV-visible, FTIR and TEM studies. The absorption band observed at 289.9 nm with an energy band gap of 4.28 eV is confirmed by UV- visible spectroscopic studies. FTIR result confirms the presence of functional groups in ZnO nanoparticles. The particle size was found to be around 20 nm with hexagonal Wurtzite structure as evidenced from TEM results. Thus, it is exemplified that the ZnO nanoparticles synthesized in this research work acts as a promising candidate for future biological applications. REFERENCES 1) Y.Y. Loo, B.W. Chieng, M. Nishibuchi, S. Radu, “Synthesis of silver nanoparticles by using tea leaf extract from Camellia Sinensis”, Int. J. Nanomed., Vol. 7, (2012), 4263-4267. 2) J. Suresh, G. Pradheesh, V. Alexramani, M. Sundrarajan, S.I. Hong, “Green synthesis and characterization of zinc oxide nanoparticle using insulin plant (Costus pictus D. Don) and investigation of its antimicrobial as well as anticancer activities”, Adv. Nat. Sci.: Nanosci. Nanotechnol., Vol. 9, (2018), 015008. 3) L.F.A. Anand Raj, E. Jayalakshmy, “Effect of zinc oxide nanoparticle produced by Zingiber Officinale against pathogenic bacteria”, J. Chem. Pharmaceutical Sci., Vol. 8, (2015), Issue 1. 4) C. Thirunavukkarasu, R. Archana, S. Sharmila, B. Janarthanan, J. Chandrasekaran, “Preparation and characterization of zno nanoparticles using Moringa Oleifera extract by green synthesis method”, Asian Journal of Phytomedicine and Clinical Research, Vol. 4, (2016), 121 - 132. 5) T.S. Anvekar, V. Rajendra Chari, H. Kadam, “Green synthesis of ZnO nanoparticles, its characterization and application”, Mater. Sci. Res. India, Vol.14, (2017), 153-157. 6) S.S.M. Hassan, W.I.M.E. Azab, H.R. Ali, M.S.M. Mansour, “Green synthesis and characterization of ZnO nanoparticles for photocatalytic degradation of anthracene”, Adv. Nat. Sci.: Nanosci. Nanotechnol., Vol. 6, (2015), 045012. 7) S. Goutam, A.K.Yadav, A. Jyoti Das, “Coriander extract mediated green synthesis of zinc oxide nanoparticles and their structural, optical and antibacterial properties”, J. Nanosci. Tech., Vol. 3, (2017), 249-252. 8) G. Bhumi, N. Savithramma, “Biological synthesis of zinc oxide nanoparticles from Catharanthusroseus G. Don. Leaf extract and validation for antibacterial activity”, Int. J. Drug Dev. & Res., Vol. 6, (2014), 208-214. 9) S. Narendhran, R. Sivaraj, “Biogenic ZnO nanoparticles synthesized using L. aculeata leaf extract and their antifungal activity against plant fungal pathogens”, Bull. Mater. Sci., Vol. 39, (2016), 1–5. 10) J. Santhoshkumar, S.Venkat Kumar, S. Rajeshkumar, “Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen”, Resource Efficient Technologies, Vol. 3, (2017), 1–7. 11) S. Kavitha, M. Dhamodaran, Rajendra Prasad, M. Ganesan, “Synthesis and characterisation of zinc oxide nanoparticles using terpenoid fractions of Andrographis paniculata leaves”, Int. Nano Lett. Vol. 7, (2017), 141–147. 12) A. Geetha, R. Sakthivel, J. Mallika, R. Kannusamy, R. Rajendran, “Green synthesis of antibacterial zinc oxide nanoparticles using biopolymer Azadirachta indica Gum”, Oriental J. Chem., Vol. 32, (2016), 955-963. 13) D.S. Chauhan , C.S.A. Gopal, D. Kumar, N. Mahato, M.A. Quraishi, M.H. Cho, “Microwave induced green synthesis of nanostructured ZnO as promising antibacterial agent”, Glob. J. Nanomed., Vol. 2, (2017), 001-005.
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