Recombinant DNA technology involves combining DNA from different sources by using restriction enzymes and plasmids. This allows scientists to [1] cure or treat diseases by introducing therapeutic genes into organisms, [2] genetically modify foods, and [3] better understand genetics. Bacteria are commonly used because they reproduce quickly and it is easy to introduce foreign DNA. The process involves isolating genes, cutting DNA with restriction enzymes, combining DNA fragments, and inserting the new recombinant DNA into bacteria. This can be used to produce human insulin in E. coli for diabetes treatment.
Genetic engineering principle, tools, techniques, types and applicationTarun Kapoor
Basic principles of genetic engineering.
Study of cloning vectors, restriction endonucleases and DNA ligase.
Recombinant DNA technology. Application of genetic engineering in medicine.
Application of r DNA technology and genetic engineering in the products:
a. Interferon
b. Vaccines- hepatitis- B
c. Hormones- Insulin.
Polymerase chain reaction
Brief introduction to PCR
Basic principles of PCR
Genetic engineering principle, tools, techniques, types and applicationTarun Kapoor
Basic principles of genetic engineering.
Study of cloning vectors, restriction endonucleases and DNA ligase.
Recombinant DNA technology. Application of genetic engineering in medicine.
Application of r DNA technology and genetic engineering in the products:
a. Interferon
b. Vaccines- hepatitis- B
c. Hormones- Insulin.
Polymerase chain reaction
Brief introduction to PCR
Basic principles of PCR
Gene probes, preparation of chimeric DNA molecules, cloningHanzala Rahaman
This is a presentation of Biochemistry and Molecular Biology entitled "Gene probes, preparation of chimeric DNA molecules, cloning" which was fundamental for my Advanced Biochemical Technique course.
Introduction to RDT methods in genetic engineeringCollege
This presentation gives a small review about the RDT based methods used generally in genetic engineering. This presentation include various images about the technique, which will help the user in understanding the concepts easily.
Gene probes, preparation of chimeric DNA molecules, cloningHanzala Rahaman
This is a presentation of Biochemistry and Molecular Biology entitled "Gene probes, preparation of chimeric DNA molecules, cloning" which was fundamental for my Advanced Biochemical Technique course.
Introduction to RDT methods in genetic engineeringCollege
This presentation gives a small review about the RDT based methods used generally in genetic engineering. This presentation include various images about the technique, which will help the user in understanding the concepts easily.
** If this presentation wins, all proceeds will go to ChristmasFuture TM **
This Christmas, change the world for good! ChristmasFuture empowers you to give a new kind of holiday gift—one that helps eradicate extreme poverty and changes the way we all think about giving.
I heard about this contest from slideshare
PPT on the problem of food security in India and related issues such as hunger,famine,public distribution system in india based on the Economics textbook for class 9th from NCERT.
Is the cloning a way for surving of humankind?
The cloning is an extraordinary method used nowadays quite often. But it presents a lot of prejudices and a lot of people think that it is too dangerous. So I have decided to present different types of cloning and their contribution for our life.
At the end, I show some disadvantages as well and I reply to the question asked in the title.
Just discover my work and ask me some questions, because I would like to show you my point of view.
#Scichallenge2017
Source: https://www.slideshare.net/sameer99932/gene-cloning
https://www.slideshare.net/kmm49/stem-cell-research-and-therapeutic-cloning?next_slideshow=1
https://www.neb.com/applications/cloning-and-synthetic-biology
https://www.ncbi.nlm.nih.gov/books/NBK21498/
https://www.neb.com/applications/cloning-and-synthetic-biology/pcr-cloning
http://learn.genetics.utah.edu/content/cloning/whatiscloning/
https://www.google.fr/search?q=cloning+dna&source=lnms&tbm=isch&sa=X&ved=0ahUKEwjLmtuA0vTSAhVCwxQKHTfYDWAQ_AUIBigB&biw=1366&bih=662#imgrc=cwNtgpdBcbf5dM:
https://www.google.cz/search?q=cloning+dna&source=lnms&tbm=isch&sa=X&ved=0ahUKEwj75oyS1vTSAhXHvBQKHd0jAdEQ_AUIBigB&biw=1366&bih=662#tbm=isch&q=cloning+&*&imgrc=dRmArnbnO_G5oM:
https://www.sciencelearn.org.nz/resources/2031-dna-cloning
google pictures
https://www.camoinassociates.com/biotechnology-market-trends-what-economic-developers-need-know-1
https://www.google.cz/search?q=cloning+dna&source=lnms&tbm=isch&sa=X&ved=0ahUKEwj75oyS1vTSAhXHvBQKHd0jAdEQ_AUIBigB&biw=1366&bih=662#tbm=isch&q=biotechnologie&*&imgrc=W7gqplQ-6DXMFM:
https://www.google.fr/search?q=beef+burger+stem+cell&source=lnms&tbm=isch&sa=X&ved=0ahUKEwiCm-ek5fTSAhVrB8AKHXVrBJ8Q_AUIBigB&biw=1366&bih=662#imgrc=iBUVaE_QE9PAfM:
http://learn.genetics.utah.edu/content/cloning/whyclone/
The Discovery of DNA Essay
DNA EXTRACTION Essay
DNA Essay
DNA Essay example
DNA Essay
Dna Replication Essay
Dna Replication Essay examples
Dna Essay
DNA Profiling Essay example
Dna And Sequence Of Dna Essay
Essay On DNA Discoveries
DNA Forensic Essay
DNA testing Essay
Dna And Protein Synthesis Essay
Dna Discovery Essay
Dna Editing Research Paper
Dna Essay
DNA Essay
Dna Essay
3. What the heck is Recombinant
DNA?
Recombinant DNA is what you get when
you combine DNA from two different
sources.
For example:
Mouse + Human DNA
Human + Bacterial DNA
Viral + Bacterial DNA
Human + (other) Human DNA
(It’s sort of like Frankenstein-DNA!)
4. Why Make Recombinant DNA?
Recombinant DNA Technology May
Allow Us To:
• Cure or treat disease
• Genetically modify our foods to increase
flavor, yield, nutritional value or shelf-life
• Better understand human genetics
• Clone cells or organs
5. Molecular Biology’s Best Friends: Bacteria
Why use bacteria?
• They’re relatively simple organisms.
• They reproduce very quickly and asexually (this
means that the “daughter” cells will contain the
exact same DNA as the “parent” cell).
• It’s pretty easy to get DNA back into the bacteria
after you’ve changed it.
• We can mess around with their DNA and kill a
lot of them during our experiments and nobody
gets mad. ;-)
8. Restriction Enzymes:
Molecular Scissors
A restriction enzyme (RE) is a specialized protein that
cuts DNA in a very specific place.
• Different REs cut at different places along the
nucleotide sequence.
10. Restriction Enzymes, Cont.
• Any piece of
DNA cut with
a certain
restriction
enzyme will
“stick” to any
other piece
cut with that
same RE,
even if they
come from
different
sources.
11.
12. What do
you notice
about these
“restriction
sites”
(places
where
restriction
enzymes
cut)?
16. Step 1:
Isolate (find) the human gene
responsible for producing insulin
and decide where you want to put it.
In this case, we decide to put our human
DNA into the plasmid of E. coli, a very
common bacterium.
17. Step 2:
Get the bacterial (plasmid) DNA
out of the E. coli. We do this by basically
exploding them.
Step 3:
Cut your human DNA and bacterial DNA
with the same restriction enzyme.
18.
19. Step 4:
Mix the cut human DNA, which
contains the insulin gene, with the
cut bacterial DNA.
They’ll stick together because they
were cut with the same restriction
enzyme.
20.
21. Step 5:
Get your new recombinant
plasmid back into the bacteria.
This is easy because bacteria
will take in DNA that’s floating
around near them. We call this
“transformation”.
22.
23. Step 6
• Find the bacteria that have taken up your
recombinant plasmid amongst the riff-raff in
the petri dish.
Remember how I
said that many
plasmids contain a
gene that makes
them resistant to
antibiotics? How
might you use that
to find your special
bacteria?
24.
25. Voila!!
Now your E. coli will use its new DNA
to make human insulin!
Because they reproduce so quickly, you’ll soon
have thousands, millions, or billions of human
insulin making machines.
By filtering out the bacteria after they’ve made
insulin, you’ve got clean human insulin that can
be packaged and given to diabetic patients.
YAY!