This document discusses various chemical reagents used in chemical analysis and reactions. It provides a classification and overview of common reagents for aldehydes, ketones, quinones, amines, and redox reactions. For each reagent, it describes the basic principle and applications. Some example reagents covered include PDAB, ninhydrin, 2,6-dichloroquinone-4-chloroimide, MBTH, Bratton-Marshall reagent, and 2,3,5-triphenyl tetrazolium chloride. The document is intended as an informational guide on reagents used in chemical and pharmaceutical analysis.
Notes* for the subject 'Advanced Pharmaceutical Analysis'Sanathoiba Singha
As per the syllabus prescribed by Rajiv Gandhi University of Health Sciences, Karnataka, for M. Pharm (Pharmaceutical Analysis) 1st semester.
*not all topics have been included in this collection of notes.
In this slide I have given brief knowledge about types of preservatives. This slide is recommended to students who are new to this particular topic or those who want notes for examination. I hope you will get benefit from this slide. Do comment for any improvement or want slides that i should prepare for you.
Method Validation - Limit of Detection, Quantitation limits and Robustnesslabgo
Prepared By: Shruti Vij (Senior Analyst) , Geeta Mathur(Senior Scientist) ,Khushbu ( Analyst)
This slide show contains detailed explanation of three characteristics of method validation- Limit of detection, Quantitation limits and Robustness. Limit of detection is the minimum amount of substance that can be detected but not measured, quantitation limit is the minimum amount of substance which can be detected and measured. Common approach to these procedures- signal to noise ratio has also been covered. Robustness is a characteristic which determines a method’s reliability when deliberate variations are induced in parameters.
In this slide contains introduction, principle, precautions, solution and assay method for vitamin B series.
Presented by: P. VENKATESH (Department of pharmaceutical analysis),
RIPER, anantapur
As we all know chromatographic fingerprinting of botanicals is a quite recent concept. This presentation will help to the beginners to understand basic thories and fundamantals of thin layer chomatography. The presentation will also provide basic experiemental understanding to perfrom HPTLC fingerprinting of samples/extracts/formulations.
Reagents & reactions in estimation of pharmaceuticalsudaya rajitha
Reagents are used for the qualitative and quantitative determination of the pharmaceutical preparations, as they react with particular groups and increase their detectability by impart colour to the compounds.
Notes* for the subject 'Advanced Pharmaceutical Analysis'Sanathoiba Singha
As per the syllabus prescribed by Rajiv Gandhi University of Health Sciences, Karnataka, for M. Pharm (Pharmaceutical Analysis) 1st semester.
*not all topics have been included in this collection of notes.
In this slide I have given brief knowledge about types of preservatives. This slide is recommended to students who are new to this particular topic or those who want notes for examination. I hope you will get benefit from this slide. Do comment for any improvement or want slides that i should prepare for you.
Method Validation - Limit of Detection, Quantitation limits and Robustnesslabgo
Prepared By: Shruti Vij (Senior Analyst) , Geeta Mathur(Senior Scientist) ,Khushbu ( Analyst)
This slide show contains detailed explanation of three characteristics of method validation- Limit of detection, Quantitation limits and Robustness. Limit of detection is the minimum amount of substance that can be detected but not measured, quantitation limit is the minimum amount of substance which can be detected and measured. Common approach to these procedures- signal to noise ratio has also been covered. Robustness is a characteristic which determines a method’s reliability when deliberate variations are induced in parameters.
In this slide contains introduction, principle, precautions, solution and assay method for vitamin B series.
Presented by: P. VENKATESH (Department of pharmaceutical analysis),
RIPER, anantapur
As we all know chromatographic fingerprinting of botanicals is a quite recent concept. This presentation will help to the beginners to understand basic thories and fundamantals of thin layer chomatography. The presentation will also provide basic experiemental understanding to perfrom HPTLC fingerprinting of samples/extracts/formulations.
Reagents & reactions in estimation of pharmaceuticalsudaya rajitha
Reagents are used for the qualitative and quantitative determination of the pharmaceutical preparations, as they react with particular groups and increase their detectability by impart colour to the compounds.
Paul Graham, the founder of startup incubator YCombinator, put it best when he described LISP as his old company's secret weapon. Think about, if you use all of the same tools as everyone else, how do you expect to achieve better results?
Clojure is a LISP language created in 2009 by Rich Hickey. Built initially on the Java Virtual Machine (JVM) it has since been ported to run on Microsoft and JavaScript. (That's right the browser). Clojure gives you all of the power and stability of the JVM without the clunkiness of Java.
Most developers have never worked with a functional language before and many who have found the use of parenthesis instead of braces intimidating. Don't worry. Once it is broken down to you, I think you will see the beauty of it.
In this fast and fun session, we will build an app using Clojure. We will enhance it, test it and explore why functional is a better programming model than OOPs. We will even explore why such programs are better at multitasking than object oriented ones.
Medical Supplies Budgeting and Consumable Price Benchmarking l MD BuylineMD Buyline
Webinar: Understanding the Impact of Consumable Costs that Exceed Their Medical Capital Investments presented by MD Buyline and hosted by HFMA.
MD Buyline's experts share examples of consumables in the clinical laboratory, reagent rentals, pulse oximetry, bronchial thermoplasty, temperature management and cardiac ablation.
http://info.mdbuyline.com/l/18032/2013-10-01/cbkcp
Application of High Performance Thin Layer Chromatography with Densitometry f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
These are the slides for the talk given in the Clojure Meetup user group. There are notes added about each slides, sadly, the videos can't be played but notes cover what is shown.
If you have any questions, send me an e-mail, you can find it in the last slide.
Industrial enzymes in the synthesis of drugs/ intermediatesPHARMA IQ EDUCATION
Importance of the following enzymes for the synthesis of drugs
1. introduction
2. penicillin acylase
3. lipase
4. oxidoreductase
5. transaminase
6. protease
Conclusion
References
Thanks
Ninhydrin Based Visible Spectrophotometric Determination of GemigliptinRatnakaram Venkata Nadh
A simple method is described to determine the amount of gemigliptin in bulk and tablet formulation by visible spectrophotometry. Basis of the proposed method is the reaction of the primary amine present on gemigliptin with ninhydrin in alkaline pH (alkaline borate buffer) medium to produce a purple color (Ruhemann’s purple) which has maximum absorption at 558 nm. The method was validated as per the current ICH guidelines. The obtained regression equation (y = 0.0148x+0.0011) in the range of 5-30 μg mL-1 has a good correlation coefficient (> 0.999). As the method does not require any separation, it is rapid and simple. The recovery levels of the drug were in the range of 99.73 – 99.96. This method is a green method as it no organic solvents were employed
Ninhydrin Based Visible Spectrophotometric Determination of GemigliptinRatnakaram Venkata Nadh
A simple method is described to determine the amount of gemigliptin in bulk and tablet formulation by visible spectrophotometry. Basis of the proposed method is the reaction of the primary amine present on gemigliptin with ninhydrin in alkaline pH (alkaline borate buffer) medium to produce a purple color (Ruhemann’s purple) which has maximum absorption at 558 nm. The method was validated as per the current ICH guidelines. The obtained regression equation (y = 0.0148x+0.0011) in the range of 5-30 μg mL-1 has a good correlation coefficient (> 0.999). As the method does not require any separation, it is rapid and simple. The recovery levels of the drug were in the range of 99.73 – 99.96. This method is a green method as it no organic solvents were employed
APPLICATION OF TECHNIQUES TO BIOMOLECULES - pdf.pdfHermain Shaikh
Hey, this pdf is all about "Applications and Techniques to Biomolecules" e.g. protein and amino acid analysis, peptide sequencing, the reaction of specific amino acid, colorimetric method, fluorimetric method, and microbiological method.
Method Development and Validation of Clopidogrel Bisulphate by Reverse Phase-...SriramNagarajan15
A new, simple sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Clopidogrel bisulphate was chromatographed on a reverse phase C18column (150 mm x 4.5 mm, i.d 5μm) in a mobile phase consisting of acetonitrile and phosphate buffer (pH: 3.0) in the ratio of 60:40 % v/v. The mobile phase was pumped at a flow rate of 1 ml/min with detection at 224 nm. The detector response was linear in the concentration of 50-150 μg /ml. The limit of detection and limit of quantitation was found to be 1.3 and 4.2 µg/ml, respectively. The intra and inter day variation was found to be less than 2%. The mean recovery of the drug from the solution was 99.79%. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Key words: Clopidogrel bisulphate, RP-HPLC, Validation, Accuracy, Precision.
Method Development and Validation of Clopidogrel Bisulphate by Reverse Phase-...SriramNagarajan15
A new, simple sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Clopidogrel bisulphate was chromatographed on a reverse phase C18column (150 mm x 4.5 mm, i.d 5μm) in a mobile phase consisting of acetonitrile and phosphate buffer (pH: 3.0) in the ratio of 60:40 % v/v. The mobile phase was pumped at a flow rate of 1 ml/min with detection at 224 nm. The detector response was linear in the concentration of 50-150 μg /ml. The limit of detection and limit of quantitation was found to be 1.3 and 4.2 µg/ml, respectively. The intra and inter day variation was found to be less than 2%. The mean recovery of the drug from the solution was 99.79%. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of Clopidogrel bisulphate in bulk drug and pharmaceutical formulation. Key words: Clopidogrel bisulphate, RP-HPLC, Validation, Accuracy, Precision.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
7. To detect indole production in cell.
Rapid identification of bacteria containing tryptophan enzyme system.
Analysis of flavonoids.
Identification of micro organism in urine medium
Study of narcotic drugs
APPLICATIONS
9. APPLICATIONS
Quantitative estimation of antibiotics
Bacitracin
Cycloserine
Polymixin B
Estimation of drugs like
Folic acid
Methyldopa
Cephalenin
Salbutamol
For protein analysis
In bone analysis
In finger print detection
11. Colorimetric determination of pyridoxine( indo phenol ester)
Chloramphenicol can be estimated at 555nm it produce purple color
Procaine HCl at 550nm, it produce pink color.
Folic acid can measure at 550nm.
Sulphonamides can also estimated.
APPLICATIONS
13. It is used in the determination of the following
Phenols
Formaldehyde
Aldehydes
Poly hydroxyl compounds
Primaquine- pink(510nm)
Adrenergic Drugs like Salbutamol, Pholedine
APPLICATIONS
14. In diazonium salt electrophilic reaction takes place
PRINCIPLE:
Sulphonamide diazotization Diazo compound + BMR
coupling
Pink color
Diazo compound BMR coloured complex
BRATTON MARSHALL REAGENT
15. Estimation of sulphonamides
Eg: sulphamethaxazole- yellow color at 445nm
sulphamethazine- pink color
In clinical analysis; solution deproteised with alcohol
chloramphenicol- purple color at 555nm
procaine - pink color at 550nm
Application in quantitative
inorganic analysis of nitrates, nitrite and sulphonamide in blood.
APPLICATIONS
Chloramphenicol
Procaine
16. 2,3,5-TRIPHENYL TETRAZOLIUM CHLORIDE
Redox indicator.
PRINCIPLE:
Steroids(alpha-keto grp)
succinate dehydrogenase
TTC (white colour) 1,3,5-triphenyl formazan (red
colour)
{Water soluble } {water insoluble}
𝑅𝐻2 Dehydrogenase R + 2𝐻+
+2𝑒−
(Substrate) ( Oxidised substrate)
Tri-phenyl formazan is formed and extinction in measured in blank reagent
17. For reducing sugars and also distinguish between
alpha-ketone and simple aldehyde.
For reducing plant and animal tissues.
Molybdenum in the plant extract at 250nm.
Autopsy_ pathology of Heart tissue.
APPLICATIONS
