1. TPTZ reagent is used to estimate various drugs and compounds based on their ability to reduce ferric ions to ferrous ions which then form a colored complex with TPTZ. 2. The document describes the synthesis and properties of TPTZ reagent and provides procedures to estimate compounds like ethacridine lactate, vitamin E, N-acetyl-L-cysteine, and uric acid using their reaction with TPTZ reagent. 3. The estimation procedures involve the compounds reducing ferric ions to ferrous ions in the first step, followed by the ferrous ions forming a colored complex with TPTZ reagent that can be measured spectrophot