Prosite

Prosite

• 1.
  A Presentation on Prosite in Biotechnology By Rashi Srivastava Roll No.:1900520545007 Institute of Engineering & Technology An Autonomous Constituent Institute of Dr A.P.J. Abdul Kalam Technical University , Lucknow, U.P. February, 2020
• 2.
  Introduction • PROSITE isa method of determining what is the function of uncharacterized proteins translated from genomic or cDNA sequences. • It consists of a database of biologically significant sites and patterns formulated in such a way that with appropriate computational tools it can rapidly and reliably identify which known family of protein (if any) the new sequence belongs to.
• 3.
  Continued... • In somecases the sequence of an unknown protein is too distantly related to any protein of known structure to detect its resemblance by overall sequence alignment, but it can be identified by the occurrence in its sequence of a particular cluster of residue types which is variously known as a pattern, motif, signature, or fingerprint. • Currently, most of the new PROSITE entries are centered around profiles and are developed by the PROSITE collaborators at the SIB Swiss Institute of Bioinformatics in Geneva and Lausanne.
• 4.
  Database convention • Generalstructure: The PROSITE database is composed of two ASCII (text) files. The first file (PROSITE.DAT) is a computer readable file that contains all the information necessary to programs that will scan sequence(s) with patterns and/or matrices. The second file (PROSITE.DOC) contains textual information that fully documents each pattern and profile
• 5.
  Continued. Data file structure:
• 6.
  Methodology Prosite methodology Patterns development Patterns from the literature Stepsin the development of a new pattern Profiles development Repeat identification