Presumptive tests are used at crime scenes to determine if a sample could be evidence such as blood, semen, or drugs. They are quick, cheap, and easy but not confirmatory. Confirmatory tests are used in a lab to confirm that a sample is a specific type of evidence and are more expensive and time-consuming. Investigators may use lights or chemical reagents like luminol or phenolphthalein to detect blood evidence not visible to the naked eye at a crime scene.

1. Forensic Tests Vide

2. Presumptive Tests
1. Determines if a sample COULD
BE evidence. They are quick,
cheap, and easy tests
2. Usually a simple color change
test. Can be done in the field or
at crime scene
3. Some preliminary tests:
– Blood, semen, bodily fluids
– Illegal drugs
• Examples:
– Luminol (blood)
– Acid phosphatase (semen)
– Cobalt thiocyanate (cocaine)

3. Confirmatory Tests
1. CONFIRMS that the sample IS that specific
evidence. They are expensive, need special
equipment, and can take a long time to
complete
2. Must be done in the laboratory
3. Tests for:
– Blood, semen, urine, bodily fluids
– Illegal drugs
– DNA
– Poisons
1. Example tests:
– Takayama Test (human blood)
– Micrsocope- Christmas Tree stain (semen)
– GC/MS- Gas Chromatography/Mass Spectrometry
(cocaine)

4. Light Source
Investigators will first examine the crime scene to look for areas
that may contain blood. They may use a high-intensity light or
UV lights to help them find traces of blood as well as other bodily
fluids that are not visible under normal lighting conditions.
How is blood evidence detected at a crime scene?
Blood Reagent Tests
These presumptive tests are used to detect blood at crime scenes
based upon the properties of hemoglobin in the blood. Further
tests at the crime lab can determine if it is human blood or not.
Examples:
• Phenolphthalein is a chemical that is still utilized today and is
usually referred to as the Kastle-Meyer test and produces a pink
color when it reacts with hemoglobin. We will use!
•HemaStix is a strip that has been coated with
tetramethylbenzidine (TMB) and will produce a green or blue-
green color with the presence of hemoglobin. We will use!
Kastle-Meyer Test
HemaStix

5. • Luminol We will use
This chemical is used by crime scene investigators to locate traces of
blood, even if it has been cleaned or removed.
Investigators spray a luminol solution is throughout the area under
investigation and look for reactions with the iron present in blood,
which causes a blue luminescence.
One problem is that other substances also react, such as some metals,
rust, paints, cleaning products, and plant materials (such as
horseradish). Another problem is that the chemical reaction can
destroy other evidence in the crime scene.
Luminol
Reaction
LCV or Leuco Crystal Violet, is one type of chemical process that is used for blood
enhancement. Using this test helps to make the blood evidence more visible so it can be
photographed and analyzed.
• Fluorescein
This chemical is also capable of detecting latent or old blood, similar to
luminol. It is ideal for fine stains or smears found throughout a crime
scene. After the solution has been sprayed onto the substance or area
suspected to contain blood, a UV light and goggles are used to detect
any illuminated areas, which appear greenish-white if blood is
present. It may also react to many of the same things as luminol
(copper and bleach).
Fluorescein
Reaction in
UV Light

7. POSITIVE
A blood test is POSITIVE when the test response is
POSITIVEand the sample IS BLOOD.
4. POSITIVE AND NEGATIVE TESTS
NEGATIVE
A blood test is NEGATIVE when the test response is
NEGATIVE and the sample IS NOT BLOOD.
FALSE NEGATIVE
A blood test is NEGATIVE when the test response is
NEGATIVE and the sample IS BLOOD.
FALSE POSITIVE
A blood test is POSITIVE when the test response is POSITIVE
and the sample is NOT BLOOD.