This document discusses plant breeding and summarizes:
1. The structure and components of flowers including the gynoecium, androecium, perianth, and receptacle. It describes the parts of carpels, stamens, petals, and sepals.
2. Tools used in plant breeding such as forceps, scissors, pointers, tags, and bags that are used for emasculation, crossing, and labeling flowers.
3. Techniques for emasculation and crossing in important crops like maize including hand emasculation, alcohol treatment, and methods for selfing and crossing.
Selfing and crossing techniques in crop plants and Breeders KitRajendragouda Patil
Studying about, Selfing and crossing techniques in crop plants and Breeders Kit,helps to students and researchers to select appropriate techniques for selfing and crossing in different crops.
The plant breeder frequently uses different tools/ instruments and materials to carry out selfing, artificial crossing and for taking field observations.
From the forgoing presentation, it can be concluded that breeding characters viz., flowering period, inflorescence, time of flower opening, time of anther dehiscence, time of stigma receptivity, pollinating agent ,time of visitor of pollinating agent and fruit set (%) in tropical species are required to be studied as they are vital for any improvement and eco-environmental planning purposes. It also throws light on how species adopts itself along with the phenomenon of speciation and reproductive isolation. From these characters we can introduce new variety which is essential for further evaluation and also the identification of the interactions between biological factors, such as animal, plant species, and non-biological factors, like temperature, RH, rain and wind, helps us to elaborate management and conservation plans for the ecosystems of the planet, which have become more and more necessary due to highly increased rate of deterioration of different ecosystems during the last few decades.
Selfing and crossing techniques in crop plants and Breeders KitRajendragouda Patil
Studying about, Selfing and crossing techniques in crop plants and Breeders Kit,helps to students and researchers to select appropriate techniques for selfing and crossing in different crops.
The plant breeder frequently uses different tools/ instruments and materials to carry out selfing, artificial crossing and for taking field observations.
From the forgoing presentation, it can be concluded that breeding characters viz., flowering period, inflorescence, time of flower opening, time of anther dehiscence, time of stigma receptivity, pollinating agent ,time of visitor of pollinating agent and fruit set (%) in tropical species are required to be studied as they are vital for any improvement and eco-environmental planning purposes. It also throws light on how species adopts itself along with the phenomenon of speciation and reproductive isolation. From these characters we can introduce new variety which is essential for further evaluation and also the identification of the interactions between biological factors, such as animal, plant species, and non-biological factors, like temperature, RH, rain and wind, helps us to elaborate management and conservation plans for the ecosystems of the planet, which have become more and more necessary due to highly increased rate of deterioration of different ecosystems during the last few decades.
Cotton Floral biology and breeding techniques.pdfVikraman A
This presentation gives information about breeding techniques in cotton. Conventional and non-conventional methods to produce hybrids in cotton is given crisp to easy understanding purpose
A flower, sometimes known as a bloom or blossom, is the reproductive structure found in flowering plants. The biological function of a flower is to effect reproduction, usually by providing a mechanism for the union of sperm with eggs.
In addition to facilitating the reproduction of flowering plants, flowers have long been admired and used by humans to bring beauty to their environment, and also as objects of romance, ritual, religion, medicine and as a source of food.
In this lesson you will learn about :
1) Flower - Definition & Functions
2) Floral Symmetry
3) Pedicellate & Sessile Flowers
4) Insertion of Floral Leaves on the Thalamus
5) Parts of a Flower
6) Some Important Terms related to Flowers
7) Floral Whorls - Calyx, Corolla, Androecium and Gynoecium
8) Placentation
9) Inflorescenece
I hope this document is helpful to you. Please share the document with your friends if you think this will benefit them. Get ready for the next lesson. Thanks.
Pollen or microspore culture is an in vitro technique by which the pollen grains are squeezed out aseptically from the intact anther and then cultured on nutrient medium.
the microspores, without producing male gametes, develop into haploid embryoids or callus tissue that give rise to haploid plantlets by embryogenesis or organogenesis.
this helps to acquire the whole knowledge about anther and pollen culture.
CROP IMPROVEMENT OF TREE SPICES
Spices are an essential component of human life. Seeds, roots, fruit barks, and other plant substances are used as seasoning. Spices are used to flavor and color food. Spices are widely used in Ayurvedic medicine. Spices are also high in antioxidants, heart protection, fitness regeneration, anti-inflammation, and have a variety of health benefits.
SELF INCOMPATIBILITY IN VEGETABLES
Mode of pollination is very important in plant breeding because it determines the genetic constitution, nature of gene action, ease in pollination control and stability of varieties after release. There are several mechanisms that promote cross pollination, among these self-incompatibility. Self incompatibility is defined as the prevention of fusion of fertile (functional) male and female gametes of the same plant (Gowers, 1989). Self incompatibility is a system where self-recognition and rejection is the rule that prevents inbreeding depression.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Overview on Edible Vaccine: Pros & Cons with Mechanism
Plant breeding practical
1. PLANT BREEDING PRACTICAL MANUAL
1. BOTANICAL DESCRIPTION AND FLORAL BIOLOGY
-Pavan kumar naik
What is a flower?
A flower is a functional unit concerned with reproduction. A flower can be pictured as a very
short stem (the receptacle) which holds the components of the flower in sequence at the very
tip of this stem, so they appear in the centre of the flower, c/a female organs (the gynoecium).
Next to them are the male organs (the androecium) and next to them are the petals (corolla)
and sepals (calyx).
Flower Structure
Gynoecium-(=pistil)- collective term for all the female reproductive organs of a flower
comprising one or more free or fused carpels
Carpel-One of the flower’s female reproductive organs comprising a stigma, a style
and an ovary
1. Stigma- The receptive part of the female reproductive organ on which pollen
germinates.
2. Style-the elongated part of a carpel bearing the stigma, usually at the tip.
3. Ovary-the hollow basal region of a carpel, consisting one or more ovules.
4. Ovules- the structures in the chamber of an ovary containing the egg cell, within the
embryosac.The ovules develop into the seeds after fertilization.
2. Androecium-collective term for all the male reproductive organs of a flower comprising one
or more free or fused stamens.
Stamen-the male reproductive organ of a flower consisting of an anther and a
filament.
1. Anthers- usually bilobed, contains the pollen.
2. Filament-the stalk bearing the anther
Perianth-the floral envelop usually divisible into an outer whorl (calyx) of sepals and inner
whorl of petals (corolla).
Corolla collective term for all the petals of a flower.
1. Petal- a non-reproductive accessory organ of a flower. This is sterile and
usually brightly colored, attracts insect pollinators.
Calyx collective term for all the sepals of a flower.
1) Sepal- a floral leaf or individual segment of the calyx of a flower, generally
green, that forms the outer protective layer of a flower bud.
2) Receptacle- flat, concave or convex part of the stem from which all parts of a
flower arise.
{A complete flower is one with all parts (calyx, corolla, androecium and gynoecium)
present. A flower lacking one or more of these parts is said to be incomplete. A perfect
flower is one with both androecium and gynoecium present. If either are lacking, the
flower is said to be imperfect.}
3. 2. PLANT BREEDERS KIT
FINE POINTED FORCEPS
USE: It is used for incising the floral buds and for removing the anthers from it. E.g.
Tobacco, Sesamum etc.
BUTTER PAPER BAG
USE: It is used for protecting the individual flowers or small twigs during selfing or crossing
e.g. Mungbean, Tur, Cotton, Okra, Brinjal and Earheads of Sorghum, Bajra, Wheat and
Maize.
Being white and semi-transparent it is more suitable than paper bags even enthesis or
blooming can be seen from outside
4. SMALL/ CURVED SCISSOR
USE: For cutting the small florest in cereals and small flowers in the crops like Lucerne,
Guar etc.
LONG STRAIGHT SCISSOR
USE: It is used for clipping, cutting the vegetable parts and large size floral parts in cereals
like Wheta, Sorghum, Bajra and Tobacco.
5. SHARP POINTER
USE: It is used for incising the floral parts and for removing the anthers from the crops like
bajra.
EYE LENS OR MAGNIFYING LENS
USE: For observing the reproductive parts to confirm that there should not be any part of the
anther left on the stigma or stigma is free from any foreign pollens.
6. AUTOMIZER
USE: It is used for spraying the gametocides during emasculation of flowers e.g. 57% ethyl
alcohol can be filled in it for killing the pollens of Lucerne.
U-PINS(U- CLIPS)
USE: It is used for fasting the bags on earheads or flowers to keep the bag in proper position.
7. HAIR BRUSH
USE: It is used for transferring the pollen grains in crops like Castor, Sorghum etc.
ADVANTAGE: Without injuring to stigmas or pollens, pollination is a accomplished very
smoothly.
WASHING BOTTLE
USE : It is used for filling sterilizing agent like alcohol or spirit to sterilize the scissors,
pointers, forceps and brush during crossing work.
8. SMALL WHITE TAG
USE : It is used for identifying the internal flower or a small twig during crossing
programme. The detailed information about crossing is written on it with pencil and then it is
inserted on pedicel or peduncle e.g. Cotton, Bajra, Wheat, Sorghum, Sesamum etc.
ALUMINIUM LABEL WITH WIRE
USE : It is used for tagging the flowers in fruit crops or tree species after crossing. It
is also used for identification of selected trees.
9. MUSLIN CLOTH BAG (LARGE SIZE)
USE : To cover the whole plant while selfing or crossing in the crops like Chillies,
Brinjal etc. In large sized plants like Tur it can be used for protecting individual branch also.
YELLOW SAMPLE BAG
USE : For storing the crossed seeds in small quantity.
10. BROWN PAPER BAG
USE : For selfing Bajra, Wheat, Sorghum, Castor etc.
11. 3. DIFFERENT EMASCULATION AND CROSSING TECHNIQUES IN
IMPORTANT HORTICULTURAL CROPS
Emasculation is the
process of removing
anthers from bisexual
flowers without affecting
the female reproductive
part (pistil).
Emasculation is performed
by plant breeders in
bisexual flowers to obtain
the desired variety of a
plant by crossing a
particular plant with the
desired pollen grain.
To remove the anthers, the
flowers are covered with a bag before they open. This ensures that the flower is
pollinated by pollen grains obtained from desirable varieties only. Later, the mature,
viable, and stored pollen grains are dusted on the bagged stigma by breeders to allow
artificial pollination to take place and obtain the desired plant variety.
Methods of Emasculation
HAND EMASCULATION
In species with large flowers, removal of anthers is possible with the help of forceps.
It is done before anther dehiscence. It is generally done between 4 and 6 PM one day
before anthers dehisce. The corolla of the selected flower is opened with the help of
forceps and the anthers are carefully removed with the help of forceps. Sometimes
corolla may be totally removed along with epipetalous stamens e.g. gingelly. In
cereals, one third of the empty glumes will be clipped off with scissors to expose
anthers.
SUCTION METHOD
It is useful in species with small flowers. A thin rubber or a glass tube attached to a
suction hose is used to suck the anthers from the flowers. The amount of suction used
is very important which should be sufficient to suck the pollen and anthers but not
gynoecium.
12. HOT WATER TREATMENT
In case of hot water emasculation, the temperature of water and duration of treatment
vary from crop to crop. It is determined for every species. For sorghum 42-48OC for
10 minutes is found to be suitable. In the case of rice, 10 minutes treatments with 40-
44OC is adequate.
ALCOHOL TREATMENT
In Lucerne the inflorescence immersed in 57% alcohol for10 second was highly
effective. It is better method of emasculation than suction method.
Selfing and Crossing techniques in important horticultural crops
Selfing
1. Bag the tassel before anthesis with a paper cover. Bagging of tassels should be done
in the previous day evening to avoid contamination from foreign pollen.
2. Cut the tip of the cob before the silks emerge and cover with a paper cover.
3. After 3-4 days, the silks will emerge in the form of a ‘saving brush’ in which the silks
will be of same height and stand erect.
4. Remove the cover of the tassel containing pollen and insert it over the cob after
removing the cob-cover. The inserted cover is then tied.
Crossing technique
Female parent
a. Detassel
b. Cut the tip of the cob before the silks emerge and cover with a butter paper cover.
Male parent
a. Cover the tassel before anthesis begins or as soon as the tassel emerges.
When the silks emerges in the female parent in the form of a brush, pollination is done by
transferring the freshly shed pollen cover form the male parent and inserting it over the cob of
the female parent after removing the cover from the cob.
The details like date of pollination, parentage and breeding programme to be carried out are
clearly written by water proof pencil. The date or pollination will be one day later than the
date of tasselling. Pollination should be completed within one week of silk
emergence. Isolation distance for maize = 400M.
13. 4. BREEDING OBJECTIVES FLORAL BIOLOGY, SELFING-
EMASCULATION CROSSING TECHNIQUES IN TOMATO, BHENDI
AND CHILLIES.
14.
15. BRINJAL
Floral biology
Brinjal flowers are large, violet coloured and solitary or in clusters of two or
more.
Calyx: sepals 5, united
Corolla: petals 5, united, usually cup shaped
• Androecium : stamens 5, alternate with corolla; Gynoecium: carpels are united,
ovary superior. The hypogynus gynoecium is syncarpus.
• The perfect flowers are borne singly and opposite to the leaves. In brinjal,
heterostyly is a common feature. Four types of flowers have been reported
depending on the length of styles, viz. (i) long-styled with large ovary, (ii)
medium-styled with medium size ovary, (iii) Pseudoshort-styled with
rudimentary ovary and (iv) true short-styled with very rudimentary ovary
(Krishnamurthi and Subramaniam, 1954).
16. CHILLI
Habit: Casicum annnum (Capsicum and Chilli) is a perennial herb but grown as
annual.
Root: Tap root numerous profusely branched lateral roots develop. Height
extending to 1 meter.
Shoots: Main shoot is radial but lateral branches are cincinnate.
Leaves: Simple, petiole 0.5-2.5 cm long, lamina broadly lanceolate to ovate,
entire, thin, 1.5 to 12 cm long and 0.5 to 7.5 cm broad, tip acuminate, base acute.
Flowers: Flower of capsicum species are pentamerous but large fruited cultivars
have 5-7 corolla lobes. Usually borne singly and are terminal but dueto
branching they appear to be auxillary, pedicels upto 1.5 cm long.
Calyx: Campanulate, about 2 mm long enlaging and enclosing base of fruits.
Corolla: Rotate, companulate, deeply 5-partile, 8-15 mm in diameter.
Androecium: White or greenish stamens, 5-6 inserted nea base of corolla.
Occasionally anthersare yellow in colour. Stamens alternate with the petals and
correspond with them in number.
Gynoecium: Ovary superior, style simple, white or purple, stigma capitate.
Fruit: Indehiscent, many seeded bery. Variable in size, shape, colour, and degree
of pungency.
Seeds: Seeds 3-5 mm long, pale yellow.
Pollination:
Crossing time: any time of day light hours but best times are in early morning or
in late afternoon. Anthers with two pollen sacs, pollen release is from lateral
sutures longitudinally.
Out crossing: less in bell pepper types and more in chilli types.
According to various reports it varies from 7.62 to 36.8%.
17. 5. BREEDING OBJECTIVES FLORAL BIOLOGY, SELFING- EMASCULATION
CROSSING TECHNIQUES IN BOTTLE GOURD AND RIDGE GOURD.
Diagnostic characters of Bottle gourd and Ridge gourd
Vegetative Characters:
Habit: The plants are mostly succulent, trailing annual or perennial herbs. These
plants climb by means of laterally spirally coiled, simple or branched tendrils.
Roots: These plants have woody tap root system.
Stem: The stem is branched, hairy and five angular with two alternate rings of five
vascular bundles each.
Leaves: The leaves are simple, alternate, long petiole, and cordate. The leaves are
often palmately lobed. The petioles are often hollow and stipules are absent. Leaves
bear tendrils in their axils.
Floral characters:
Inflorescence: Inflorescence is cymose type. It may be axillary and bears a solitary
female flower. The male flowers may be solitary and in the form of racemes. The
plants are monoecious or sometimes dioecious as in Coccinia indica and Momordica
dioca.
Flower: The flowers are yellow or white, unisexual (rarely bisexual), actinomorphic,
pentamerous and epigynous. The thalamus forms a cup above the ovary.
Calyx: The calyx is of 5 sepals forming a tube, which is wholly adnate to the ovary in
female flowers. The aestivation is valvate.
Corolla: The corolla consists of 5 petals which are united to form a tube
(Trichosanthes). The corolla is campanulate (Coccinia, Cucurbita) form. Petals are
white or yellow in color. The petals are free in Luffa, Trichosanthes.
Androecium: The Androecium is present in the male flowers only. In female flowers it
may be represented by staminodes. The Androecium shows much variation. The
simplest condition is where 5 free stamens are present (Luffa cylindrica) with
dithecous/monothecous anthers.
In Lagenaria, Cucumis and Citrullus there are three stamens with one monothecous
anther and other two as dithecous anthers.
18. Gynoecium: The gynoecium is present only in the female flowers. It consists of 3
syncarpous carpels with completely inferior ovary. Ovary becomes spuriously three
loculed. They have only one style with three stigmas.
Fruit: The fruit is fleshy-berry with soft or hard pericarp. This type of fruit is called
pepo.
Seed: The seeds are compressed and non-endospermic.
Breeding objectives floral biology, selfing- emasculation crossing techniques in Mango.
24. 7. CALCULATION OF HETEROSIS , HETEROBELTIONSIS AND
STANDARD HETEROSIS AND INBREEDING DEPRESSION.
1. Average heterosis:
It is the heterosis where F1 is superior to mid parent value. In otherwords superior to
average of two parents.
F1 - MP
--------- x 100
MP
Where F1 = Mean of hybrid
MP = Mid parental value.
(P1 + P2) where P1 = Parent 1
MP = ---------- P2 = Parent 2
This type of heterosis is of no use in agriculture since the superiority is below the
better parent value
2. Heterobeltiosis:
Superiority of F1 over the better parent.
F1 - BP
--------- x 100
BP
Where BP = Mean of Better Parent.
3. Economic heterosis:
Superiority of the F1 compared to the high yielding commercial variety in a particular
crop.
F1 - CV
--------- x 100
CV
25. Where CV = Mean of Commercial Variety.
4. Negative heterosis:
Performance of F1 inferior to better parent / mid parent value. - e.g. Duration.