Biotechnology in capsule form. Plant biotechnology . Industry related information. Latest info. Different aspects of plant biotechnology. Cell biology. industrial biotechnology.

1. Plant cell biotechnology

2. Agriculture: The Next Revolution
 Biggest industry in the world ($1.3 trillion of products per
year)
 Plant transgenesis allows innovations that are impossible to
achieve with conventional hybridization methods
• Resistant to herbicides
• Pest resistant
• Vaccines

3. Current Uses of Biotechnology
Agriculture
• Transgenic Plants [disease resistance, drought tolerance, nutrient
use efficiency, plant-based products such as vaccines]
• Transgenic Animals
• Transgenic Microbes
Pharmaceutical
• Insulin
• Antibiotics
• Cancer therapy
Others
• Mining
• Petroleum spill clean-up with microbes

4. Unique advantages of plant
Biotechnology:
• The long history of plant breeding provides plant
geneticists with a wealth of strains that can be exploited at
the molecular level
• Plants produce large numbers of progeny; so rare
mutations and recombinations can be found more easily
• Plants have been regenerative capabilities, even from one
cell
• Species boundaries and sexual compatibility are no longer
an issue

5. Methods Used in Plant Transgenesis
• Protoplast Fusion to create plant hybrids
• Leaf Fragment Technique
• Transformation by Gene Gun Method
• Chloroplast Engineering

6. Protoplast Fusion to create plant hybrids
• Degrade cell wall with cellulase
• A cell lacking a cell wall is called a protoplast
• The protoplasts from different species of plants can be fused together
to create a hybrid
• The fused protoplasts grow in nutrient agar for a few weeks
• The colonies are then transferred to media to induce root and shoot
growth

7. Leaf Fragment Technique
• Small discs of leaf incubated
with genetically modified
Agrobacterium Ti plasmid
• Treat with hormones to
stimulate shoot and root
development

8. Transformation by Gene Gun Method
• Blast tiny metal beads coated
with DNA into an embryonic
plant cell

9. Chloroplast Engineering
• More genes can be inserted at
one time
• Genes are more likely to be
expressed
• DNA is separate from the nucleus

10. Transformation by Agrobacterium
Agrobacterium mediate gene transfer Method
• Ti plasmid integrates into the DNA of the host cell, making it an
ideal vehicle for transferring recombinant DNA to plant cells

11. Benefits of Plant Biotechnology
• Increased crop productivity
• Enhanced crop protection
• Improved nutritional value of crop
• Environmental benefits
• Avoiding Chemical herbicide
• Virus resistance

12. THANK YOU