2. T.C.
Refers to technique of
growing plant
cells, tissues, organs, seeds
or other plant parts in a
sterile environment on a
nutrient medium In vitro
3. History
Haberlandt Realized that asepsis was necessary when culture
media enriched with organic substances metabolized by
microorganisms
In 1898 he cultured single cells on Knop’s salt solution
In his cultures the cell were able to synthesize starch as well
as increase in size
He failed in his goal to induce cell division
4. History
Despite of failures Haberlandt proposed that plant cells
are totipotent
Totipotency is ability of individual cells to express the
phenotype of whole plant from which it is derived
Haberlandt is regarded as father of plant tissue culture
5. History
Hanning(1904) Embryo culture of selected
crucifers
Robbins (1922), Kotte In Vitro culture of root tips
Laibach (1925) Use of embryo culture technique
in inter-specific crosses of Linum
6. History
White (1934-37) Successful culture of tomato roots
importance of vitamins in growth media
Snow, Gautheret (1934-1939) Importance of auxin
as growth regulator
Gautheret, Nobecourt, White (1939) Successful
establishment of continuously growing cultures.
7. History
Van Overbeek (1941) Use of coconut milk
containing a cell division factor first time in Datura
Skoog and Tsui (1951), Miller et al (1955), In vitro
cell differentiation, discovery of Kinetin
Morel and Martin (1952) Application of micro
grafting to obtain virus free plants
8. History
Muir (1953) Isolation and culture of single cells
from plants
Skoog and Miller (1957) Discovery of principle of
hormonal control of the organ formation in tissue
culture
9. History
Reinert and Steward (1958-59) First report on
somatic embryogenesis
Jones et al (1960) Hanging drop method of cell
culture
Bergmann (1960) Bergmann cell culture
technique
10. History
E C Cocking (1960) Isolation of protoplasts by
enzymatic degradation method
Murashige and Skoog (1962) Development of
MS media
Guha and Maheshwawari (1964) Production of
First haploid plant by anther culture
11. History
Power et al (1970) Protoplast fusion
Takabe et al (1971)Rgeneration of first plant
from protoplast
Carlson et al (1972) First report on inter-specific
hybridization through protoplast fusion
