Oxytocin is a hormone produced in the hypothalamus that facilitates childbirth and lactation. Bioassays are used to evaluate the potency of oxytocin in biological products. Three common bioassay methods are described: the isolated uterus method using rat uteri to measure contractions, the chicken blood pressure method measuring oxytocin's effect on blood pressure, and the milk ejection method using lactating rabbits or rats to measure milk flow. Each method involves administering standard and test oxytocin preparations and measuring a physiological response that is compared to evaluate potency. Bioassays provide important quality control for oxytocin products.