Oxytocin is a hormone produced in the hypothalamus that stimulates contractions of the uterus during childbirth and the mammary glands to produce milk during breastfeeding. It plays an important role in bonding between mothers and their children. There are four main methods used to test the potency of oxytocin in biological assays: by measuring its ability to decrease blood pressure in chickens, induce contractions in isolated rat uteri, increase milk ejection pressure in lactating rats, and elevate blood pressure through vasopressor activity in rats. Each method involves carefully preparing test animals, administering doses of both a standard and test oxytocin preparation, and recording and statistically analyzing the biological responses.
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
In this slide contains introduction, role, mechanism, and assay of oxytocin.
Presented by: P.PAVAN KALYAN (Department of pharmaceutical analysis ).
RIPER, anantapur
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Isolation, Identification and Analysis of PhytoconstituentsDr. Siddhi Upadhyay
Isolation, Identification and Analysis of Phytoconstituents
a) Terpenoids: Menthol, Citral, Artemisin
b) Glycosides: Glycyrhetinic acid & Rutin
c) Alkaloids: Atropine,Quinine,Reserpine,Caffeine
d) Resins: Podophyllotoxin, Curcumin
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Expt. 9 Effect of atropine on DRC of acetylcholine using rat ileumVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Atropine stock and std. solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Graphical presentation of CRC/ DRC
Result and interpretation
In this slide contains introduction, role, mechanism, and assay of oxytocin.
Presented by: P.PAVAN KALYAN (Department of pharmaceutical analysis ).
RIPER, anantapur
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Isolation, Identification and Analysis of PhytoconstituentsDr. Siddhi Upadhyay
Isolation, Identification and Analysis of Phytoconstituents
a) Terpenoids: Menthol, Citral, Artemisin
b) Glycosides: Glycyrhetinic acid & Rutin
c) Alkaloids: Atropine,Quinine,Reserpine,Caffeine
d) Resins: Podophyllotoxin, Curcumin
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Expt. 9 Effect of atropine on DRC of acetylcholine using rat ileumVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Atropine stock and std. solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Graphical presentation of CRC/ DRC
Result and interpretation
Insulin bioassay is the measurement of insulin potency by biological activity measurement using a standard biological system. An often used technique is to inject insulin samples into animals, usually rats or rabbits, and then track their blood sugar levels to see how well insulin lowers blood sugar. An alternate strategy is to measure glucose absorption or metabolic reactions using cultured cells sensitive to insulin, including adipocytes or muscle cells. By giving important information on insulin potency, purity, and stability, these tests guarantee the quality of insulin products intended for therapeutic use. Bioassays continue to be necessary for insulin preparation quality control and regulatory clearance even with the development of analytical methods.
The use of algorithms & emergency boxes in obstetric emergencyWafaa Benjamin
obstetric hemorrhage Is the major cause of maternal mortality globally.
Substandard management identified as a contributor for maternal mortality in UK in 80% of the cases.
Is the major cause of mortality in Egypt ,according to the last Egyptian Maternal Mortality Report in 2001.
So we need to Work in a team, Do all needed steps, In the proper sequence of the steps,
competent emergency team should have Knowledge ,Skills , Attitude & exposed to regular Labor Ward drills.
Ready available Algorithms & Emergency Boxes are found to be helpful in emergency situations.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
3. Oxytocin
Oxy – Rapid
Tocos- Labor
Synthesized in both sexes, well recognized physiological effects
only in women.
Cyclic Polypeptide hormone - from posterior pituitary gland.
Pituitary gland consist posterior lobe which produce oxytocin
and diuretic hormone.
Neurosecretary product mainly synthesize in the cell bodies of
paraventracular nuclear of the hypothalamus.
4. ROLE OF OXYTOCIN:
• Stimulate the contraction of the uterine smooth muscle &
memory gland.
• Oestrogen progesterone & prolactin – responsible for production
of milk by mammary gland but milk ejection require oxytocin.
• Facilitates the contraction of uterus.
It is presented as a solid or solution in a solvent containing an
appropriate antimicrobial preservative such as 0.2% w/v of
chlorbutol.
Animal species - 90 - 110% stated number of units of oxytocin
activity.
Synthetic: Solid - NLT 560 units/mg Calculated with reference to
the peptide content & when liquid NLT 150 units/ml
5. Oxytocin – Mechanism of action
Neuropeptide made in hypothalamus that stimulates contractions that
expel the infant from uterus.
Responsible for milk letdown & triggered by the nipple stimulation of
suckling.
Called love & bonding hormone. It has a very special affect on mothering.
Psychologically, oxytocin promotes a feeling of well being and tranquility.
It enables the growing sense of love and attachment to the infant. The
more the infant suck the more oxytocin is produced.
In mothers it increases their attachment to their infant, promoting the
feeling of love, and makes her infant more valuable to her.
It also suppresses the fear that would normally cause her to back off from
threat.
6. Biological Assay Of Oxytocin:
Principle:
Potency is determined by comparing its activity
– Depression of BP
– Contraction of Uterus
– Milk Ejection Pressure
– Vasopressor activity
with standard preparation of oxytocin
Standard Preparation:
Consisting free dried synthetic oxytocin peptide with human
albumin citric acid (12.5 units)
Method-A (Depression Of The BP In Chicken)
Test Animals: Cockerel (young male chicken), 1.2 - 2.3 Kg,
Method-B (By Contraction Of The Rat Uterus)
Test animals: Female rat 120 – 200g
Method-C (Milk Ejection Pressure In Lactating Rat)
Test Animals: Lactating rat, 3-21 day after parturition, 300 g
Method-D Vasopressor activity
Test Animals: Male rat 300g
7. Method-A (Depression Of The BP In Chicken)
Test Animals: Cockerel (young male chicken), 1.2 - 2.3 Kg,
Anaesthetized cock-prolonged & constant high B.P
Expose gluteus primus muscle(thigh) & remove politeal artery &
crural vein.
Cannulate the popliteal artery & record B.P response
Cannulate the crural or brachial vein.
Prepare standard solution with saline. Inject 0.1 - 0.5ml
Inject 2 doses of standard solution into cannulate vein is record
B.P response
Dose should cause decrease in B.P (reqd. dose between 20-
100mUnits)
8. Interval between 2 injection, between 3-10mins depend on rate @ which
B.P return normal
Dil. test preparation with saline so as to get same response as standard
The ratio between standard & test should be equal
If animal rapidly becomes insensitive to repeated injection the soln
another must used.
Measure all responses are calculated result of the assay by std statistical
method.
9. Method-B (By Contraction Of The Rat Uterus)
Test animals: Female rat 120 – 200g
Inject 100ug of oestradiol benzoate IM into female rat before the
assay
Immediately before assay confirm by vaginal smear that rate in
oestrus or pre oestrus.
Kill rat & suspend one horn of uterus in organ bath containing a
solution of following Nacl,Kcl,Cacl2, NaHco3, Na2Hpo4, NaH2po4,
Mgcl2, Dextrose
Maintain the bath at temp at of 32 c
Bath liquid required dose between 10-50 units/ml.
Oxygenate solution with mix of 95% of O2, 5% of CO2 record -
contraction of muscle.
10. Record contraction produces by addition of two dose of std. ppn (Reqd.
Dose 10 & 50munits/ml of bath liquid)
when maximum contraction has been reached replace - bath liquid
by fresh solution.
Dose should be added at regular interval[3-5minutes]
Similarly record the contraction of test preparation as standard.
Ratio between two dose of test & two dose of std should be equal.
This ratio kept constant through out the assay.
Measure all response & calculate result of assay by standard
statistical method.
11. Method-C (Milk Ejection Pressure In Lactating Rat)
Test Animals: Lactating rat, 3-21 day after parturition, 300 g
Separate from litter & 30-60 minutes later anaesthetize (IP
Pentobarbitone Na).
Tie rat to an operating table, at 37º, by its hind legs leaving front
legs free.
Cannulate trachea with a short PE tube of i.d. 2.5 mm in such a
manner so as to ensure a free airway; apply artificial respiration
only if necessary.
Cannulate an external jugular or femoral vein with a PE tube of i.d.
0.4 mm filled with saline & closed with a pin.
Shave the skin surrounding the inguinal and abdominal teats and
excise the tip of one teat, preferably the lower inguinal teat.
12. Insert a PE tube of i.d. 0.3 mm & e.d. 0.6 mm, to a depth sufficient
to obtain appropriate measurement of pressure (3-10 mm depth),
into the primary teat duct which opens onto the cut surface and tie
firmly in place with a ligature.
Connect this cannula with a suitable strain gauge transducer
(such as that used for recording arterial BP in rat) and fill with a
3.8% w/v of Na citrate /saline contain 50 Units of heparin Na/ ml
to prevent clotting of milk.
After cannulation, inject 0.05 - 0.2 ml of this solution into teat
duct through transducer to clear milk from tip of the cannula.
(This procedure may be repeated during the assay should
obstruction arise from milk ejected into the cannula).
Clamp the strain gauge so that a slight tension is applied to the
teat and its natural alignment is preserved and connect the gauge
to a potentiometric recorder adjusted to give full-scale deflection for
an increase in milk-ejection pressure of 5.3 kPa.
13. Inject all solutions through the venous cannula using a 1-ml
syringe graduated in 0.01 ml and wash them in with 0.2 ml of
saline.
Prepare a solution of Std. & Test Ppn in saline solution so that the
volume to be injected is between 0.1 - 0.4 ml. Choose two doses of
Std Ppn such that the increase in milk-ejection pressure is about
1.35 kPa for Lr dose and about 2.7 kPa for Hr dose.
As an initial approximation, a lower dose of between 0.1 and 0.4
milli Unit and an upper dose of 1.5 to 2 times this amount may be
tried.
Choose two doses of the Test Ppn with the same inter-dose ratio,
matching effects of doses of the Std Ppn as closely as possible.
Inject four doses (2 doses of Std & 2 doses of Test) at intervals of 3-
5 minutes.
14. 2 doses of Std and 2 doses of test should be given according to
randomized block or a Latin square design & at least four
responses to each -recorded.
Measure all responses & calculate result of the assay by std
statistical methods.
Potency - 90% - 111%. Fiducial limits of error are 80% -
125%stated potency.
15. Method-D Vasopressor activity:
Test Animals: Male rat 300g
NMT 0.5 Unit /20 Units of oxytocic activity - by biological assay for
vasopressor activity- comparing activity of Test & Standard
Preparation of arginine vasopressin Freeze-dried syn. arginine
vasopressin peptide acetate with human albumin & citric acid
(supplied in ampoules containing 8.20 Units)
Inject slowly into tail vein of male albino rat weighing 300g -solution
of a suitable a- adrenoreceptor blocking agent, (10 ml/kg body
weight of solution prepared by dissolving 5 mg of phenoxy
benzamine HCl in 0.1 ml of ethanol (95%) , adding 0.05 ml of 1 M
HCl & dil to 5ml with saline .
After 18 hours, anaesthetize rat - that will maintain -prolonged &
uniform BP.
After 45-60 minutes, tie the rat on its back to the operating table by
its hind legs.
Cannulate trachea with short PE of E.D. 2.5 mm & dissect carotid
artery ready for cannulation.
16. Then cannulate the femoral vein close to the inguinal ligament.
Retract the abdominal muscles to expose the inguinal ligament.
Retract superficial pudendal vein to one side & dissect femoral vein
towards inguinal ligament from corresponding artery.
When dissecting, a deep branch reaching femoral vein must be
found & tied off to prevent bleeding during cannulation.
Tie a short PE cannula of E.D. about 1 mm into femoral vein by
two ligatures & join by a short piece of flexible tubing to a 1-ml
burette with an attached thistle funnel containing saline at about
37º.
Firmly fix wet absorbent cotton swab to thigh so as to cover
incision and cannula. At this stage inject through venous cannula
200 Units of heparin, dissolved in saline /100 g of body weight
17. Then tie in a carotid cannula of E.D. about 1 mm & connect by a
column of saline contain heparin with a pressure measuring device
such as Hg manometer of I.D. about 2-3 mm.
central & peripheral nervous system including both vagus &
associated sympathetic nerves is left intact.
No artificial respiration is necessary.
No air is injected, inject all solutions through venous cannula by
means of a 1-ml syringe & wash in with 0.2 ml of saline from
burette.
Dil extract of Std & Test Ppn with saline so that volume to be
injected is between 0.1 & 0.5 ml.
18. Choose 2 doses of the Std Ppn such that the elevation of the BP is
about 4 kPa for Lr dose & about 7 kPa but always submaximal for
higher, ratio of low to high dose being determined by response &
usually being 3-5. As an initial approximation doses of 3 and 5 M
Units may be tried.
Choose 2 doses of Test ppn with same inter-dose ratio, matching
effects of dose of Std Ppn. Inject doses at intervals of 10 - 15
minutes.
2 doses of Std & 2 doses of Test Ppn should given in randomized
block / Latin square design & 4-5 responses to each recorded.
Measure all responses & calculate result of the assay by Std
statistical methods.