This document summarizes bioassay methods for measuring the potency of insulin and vasopressin, including:
- Rabbit and mouse methods that measure the hypoglycemic or convulsive effects of test samples compared to standards.
- Rat diaphragm and fat pad methods measuring insulin's effects on glucose uptake and fat metabolism.
- Radioimmunoassay, a sensitive technique using radiolabeled antigens to quantify insulin concentrations via competitive binding.
- A suggested method for vasopressin involving cannulation of rats to measure changes in blood pressure after administration of test samples versus a reference standard.
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Isolation, Identification and Analysis of PhytoconstituentsDr. Siddhi Upadhyay
Isolation, Identification and Analysis of Phytoconstituents
a) Terpenoids: Menthol, Citral, Artemisin
b) Glycosides: Glycyrhetinic acid & Rutin
c) Alkaloids: Atropine,Quinine,Reserpine,Caffeine
d) Resins: Podophyllotoxin, Curcumin
Expt. 4 DRC of acetylcholine using frog rectus abdominis muscleVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 8 Effect of physostigmine on DRC of acetylcholine using frog rectus abd...VISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Physostigmine stock and std. solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Liquid oral topic in Industrial Pharmacy contains many topics like solution, elixirs, syrups, emulsion, and suspension. This topic includes general introduction, types, formulation, components, uses, and Quality control tests. These are also beneficial in other subjects like Pharmaceutics.
Insulin bioassay is the measurement of insulin potency by biological activity measurement using a standard biological system. An often used technique is to inject insulin samples into animals, usually rats or rabbits, and then track their blood sugar levels to see how well insulin lowers blood sugar. An alternate strategy is to measure glucose absorption or metabolic reactions using cultured cells sensitive to insulin, including adipocytes or muscle cells. By giving important information on insulin potency, purity, and stability, these tests guarantee the quality of insulin products intended for therapeutic use. Bioassays continue to be necessary for insulin preparation quality control and regulatory clearance even with the development of analytical methods.
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Isolation, Identification and Analysis of PhytoconstituentsDr. Siddhi Upadhyay
Isolation, Identification and Analysis of Phytoconstituents
a) Terpenoids: Menthol, Citral, Artemisin
b) Glycosides: Glycyrhetinic acid & Rutin
c) Alkaloids: Atropine,Quinine,Reserpine,Caffeine
d) Resins: Podophyllotoxin, Curcumin
Expt. 4 DRC of acetylcholine using frog rectus abdominis muscleVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 8 Effect of physostigmine on DRC of acetylcholine using frog rectus abd...VISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Physostigmine stock and std. solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Liquid oral topic in Industrial Pharmacy contains many topics like solution, elixirs, syrups, emulsion, and suspension. This topic includes general introduction, types, formulation, components, uses, and Quality control tests. These are also beneficial in other subjects like Pharmaceutics.
Insulin bioassay is the measurement of insulin potency by biological activity measurement using a standard biological system. An often used technique is to inject insulin samples into animals, usually rats or rabbits, and then track their blood sugar levels to see how well insulin lowers blood sugar. An alternate strategy is to measure glucose absorption or metabolic reactions using cultured cells sensitive to insulin, including adipocytes or muscle cells. By giving important information on insulin potency, purity, and stability, these tests guarantee the quality of insulin products intended for therapeutic use. Bioassays continue to be necessary for insulin preparation quality control and regulatory clearance even with the development of analytical methods.
Screening models for immunomodulatory agents:- Introduction for immunostimulants and immunosuppressant, Models for immunomodulatory agents, Screening for immunostimulants, screening for immunosuppressant
In-vitro and in-vivo methods of diuretics & antihypertensive final.pptxAishwaryaPatil697206
This ppt contains in-vitro and in-vivo preclinical methods of diuretics and antihypertensive drugs. It contains classification as well as mechanism of action.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
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TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
2. Bioassay of insulin
Standard preparation and unit:
It is pure, dry and crystalline insulin. One unit contains 0.04082
mg. This unit is specified by Ministry of Health, Government of
India and is equivalent to international unit.
Preparation of standard solution:
Accurately weigh 20 units of insulin and dissolve it in normal
saline. Acidify it with HCl to pH 2.5. Add 0.5% phenol as
preservative. Add 1.4% to 1.8% glycerin. Final volume should
contain 20 units/ml. Store the solution in a cool place and use it
within six months.
3. Preparation of test sample solution:
The solution of the test sample is prepared in the same way as the
standard solution described above.
1. Rabbit Method:
Selection of rabbits:
They should be healthy, weighing about 1800-3000 gms. They should
then be maintained on uniform diet but are fasted for 18 hrs. before
assay. Water is withdrawn during the experiment.
Standard and Sample Dilutions:
These are freshly prepared by diluting with normal NaCl solution so as
to contain 1 unit/ml. and 2 units/ml.
Doses:
The dose which can produce suitable fall in blood sugar level is
calculated for the standard.
4. Principle:
The potency of a test sample is estimated by comparing the
hypoglycemic effect of the sample with that of the std. preparation of
insulin. Any other suitable method can also be used.
Experimental Procedure:
Animals are divided into 4 groups of 3 rabbits each.
The rabbits are then put into an animal holder.
They should be handled with care to avoid excitement.
First part of the Test:
A sample of blood is taken from the marginal ear vein of each rabbit.
Presence of reducing sugar is estimated per 100 ml. of blood by a
suitable chemical method. This concentration is called ‘Initial Blood
Sugar Level’.
5. The four groups of rabbits are then given sc. injections of insulin as
follows:
• 3 Standard Dilution(I)
• 3 Sandard Dilution (II)
• 3 Test sample dilution(I)
• 3 Test sample dilution(II)
From each rabbit, a sample of blood is withdrawn up to 5 hrs. at the
interval of 1 hr. each. Blood sugar is determined again. This is known
as ‘Final Blood Sugar Level’.
Second part of the test (Cross over test) :
The same animals are used for the second part. The experiment can be
carried out after one week. Again they are fasted and initial blood
sugar is determined. The grouping is reversed, that is to say, those
animals which received the standard are given the test and those
which received the test are now given the standard.
6. Those animals which received the less dose of the standard are given the
higher dose of the test sample and vice-versa. This test is known as
‘Twin Cross Over Test’.
Mean percentage decrease in blood sugar of the first and second part is
calculated.
Mouse Method:
Mice show characteristic convulsions after s.c. inj. of insulin at elevated
temperatures. The percentage convulsions produced by the test and
standard preparations are compared.
Experimental procedure:
Minimum 100 mice weighing between 18-22 gms. of the same strain are
used. They should be maintained on constant diet. They should be
fasted 18 hrs. prior to the experiment.
Standard and sample dilutions:
Dilutions are prepared with sterile saline solution, so as to contain 0.064
units/ml. (std dilution I) and 0.096 untis/ml. (std. dilution II).
Similarly, test sample solutions are also prepared.
7. Mice are divided into 4 groups each containing 25 mice and insulin is injected
s.c. as follows:
25 standard sample dilution
25standard sample dilution
25 test sample dilution
25 test sample dilution
Mice are put in an air incubator at 33oC and observed for one and a half hr. An
air incubator with a glass front provided with six shelves is used.
The temperature is thermostatically controlled.
Two mice are kept in each of the boxes made up of perforated sheets of metal.
The mice which convulse or die are taken out of the incubator and observed.
These mice usually convulse severely but failure of the animal to upright itself
when placed on its back, should as well be considered as convulsion.
Convulsive mice may be saved by an inj. of 0.5 ml. of 5% dextrose solution.
8. Percentage convulsions produced by the test sample are compared with
those of the standard sample.
Those animals which survive may be used again for another expt. after
an interval of one week.
Rat diaphragm method:
In this method increase in glycogen content of the muscle or increase in
glucose uptake by muscle in response to insulin is taken as the index
of potency of insulin.
Rat epididymal fat-pad method:
Here, the ability of insulin to increase CO2 production by the fat-pad is
taken as the parameter for the measurement of potency of the insulin
preparation.
9. Radioimmunoassay:
It is the estimation of the concentration of the substance in a unit quantity
of preparation using radiolabelled antigens.
A number of drugs are estimated now days by radio-immunoassy
methods because these methods are highly specific and highly
sensitive.
The radioimmunoassay of insulin is based on the ability of human insulin
(unlabelled) to displace beef’s insulin (which may be labelled) from
the binding sites (i.e. antibodies). The method involves the following
steps:
I. Bovine insulin is injected into the sheep. After a week the
serum containing antibodies produced against bovine insulin is
collected form the blood of the sheep.
10. II. The serum containing antibodies is exposed to radiolabelled
insulin and the bound vs free ratio is determined.
III. The mixture of labelled antigen-antibodies is then added in
different test-tubes labelled as standard and test. About 6
concentrations of the standards are taken. They are then added
to different tubes and the bound vs free ratio is again
determined using gamma-counter.
IV. Standard curves are determined and the concentration of test
insulin is determinded using this standard curve.
11. Biological Assay for Vasopressor Activity
Principle:
The vasopressor activity is estimated by comparing the activity of the
preparation being examined with that of the Standard Preparation of
arginine vasopressin under the conditions of a suitable method of
assay.
Standard Preparation:
The Standard Preparation is the Ist International Standard for Arginine
vasopressin, established in 1978, consisting of freeze-dried synthetic
arginine vasopressin peptide acetate with human albumin and citric
acid (supplied in ampoules containing 8.20 Units), or another suitable
preparation the potency of which has been determined in relation to
that of the International Standard.
12. Suggested Method:
Inject slowly into the tail vein of a male albino rat weighing about 300 g
a solution of a suitable alpha -adrenoceptor blocking agent, for
example 10 ml per kg of body weight of a solution prepared by
dissolving 5 mg of phenoxybenzamine hydrochloride in 0.1 ml of
ethanol (95%), adding 0.05 ml of 1M hydrochloride acid and diluting
to 5 ml with saline solution. After 18 hours, anaesthetise the rat with
an anaesthetic that will maintain a prolonged and uniform blood
pressure.
After 45 to 60 minutes, tie the rat on its back to the operating table by its
hind legs.
Cannulate the trachea with a short polyethylene tube of external diameter
about 2.5 mm and dissect a carotid artery ready for cannulation.
Then cannulate the femoral vein close to the inguinal ligament.
Retract the abdominal muscles to expose the inguinal ligament.
13. Retract the superficial pudendal vein to one side and dissect the femoral
vein towards the inguinal ligament from the corresponding artery.
When dissecting, a deep branch reaching the femoral vein must be found
and tied off to prevent bleeding during cannulation.
Tie a short polyethylene cannula of external diameter about 1 mm into
the femoral vein by two ligatures and join by a short piece of flexible
tubing to a 1-ml burette with an attached thistle funnel containing
saline solution at about 37o.
Firmly fix a wet absorbent cotton swab to the thigh so as to cover the
incision and cannula.
14. At this stage inject through the venous cannula 200 Units of heparin,
dissolved in saline solution, per 100 g of body weight.
Then tie in a carotid cannula of external diameter about 1 mm and
connect by a column of saline solution containing heparin with a
suitable pressure measuring device such as a mercury manometer of
internal diameter about 2 to 3 mm.