This document describes an experiment to determine the unknown concentration of serotonin using a three-point bioassay with an isolated rat fundus strip preparation. The experiment involves constructing dose-response curves for a serotonin standard and test sample, selecting doses that elicit submaximal responses in a 1:2 ratio, and determining the test concentration using the measured responses. Rat fundus tissue is sensitive to serotonin and contracts in a concentration-dependent manner when exposed to increasing doses of the drug. The experiment aims to precisely and reliably estimate the concentration of an unknown serotonin sample through this validated bioassay method.
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 9 Effect of atropine on DRC of acetylcholine using rat ileumVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Atropine stock and std. solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 8 Effect of physostigmine on DRC of acetylcholine using frog rectus abd...VISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Physostigmine stock and std. solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 9 Effect of atropine on DRC of acetylcholine using rat ileumVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Atropine stock and std. solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 8 Effect of physostigmine on DRC of acetylcholine using frog rectus abd...VISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Physostigmine stock and std. solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 4 DRC of acetylcholine using frog rectus abdominis muscleVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 6 Study of effect of drugs on gastrointestinal motilityVISHALJADHAV100
Objective
Principle
Requirements
Preparation of Tyrode solution
Procedure
Kymograph recording of contractions
Observation table
Result and Interpretation
Expt. 2 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 4 DRC of acetylcholine using frog rectus abdominis muscleVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 6 Study of effect of drugs on gastrointestinal motilityVISHALJADHAV100
Objective
Principle
Requirements
Preparation of Tyrode solution
Procedure
Kymograph recording of contractions
Observation table
Result and Interpretation
Expt. 2 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Overview of Discussion-
Renin-Angiotensin system (RAS)
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b)Tissue (local) renin-angiotensin systems
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Other angiotensin peptides
Angiotensin receptors and transducer mechanisms
Actions of angiotensins
Pathophysiological roles of angiotensins
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Introduction
Which are the features of inflammation…?
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How PGs produce PAIN?
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How PGs produces INFLAMMATION?
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Classification of NSAIDs
Mechanism of Action: NSAIDs
Pharmacology of Individual Class of NSAIDs
Choice of NSAIDs
Analgesic combinations
Overview of Discussion-
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Discovery of SP
SP Receptor
Functions mediated by SP
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NK1 receptor antagonists
Overview of Discussion
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Generation and metabolism
Kinin receptors
Actions of kinins
Pathophysiological roles of kinins
Bradykinin antagonists
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Physiologic Distribution of Serotonin
Synthesis, Storage and Destruction
Biosynthesis of 5HT compared to CAs
Serotonin Uptake
5-HT Receptors
Actions
Pathophysiological Roles
Use
Drugs Affecting 5-HT System
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
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Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
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The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Overview on Edible Vaccine: Pros & Cons with Mechanism
Expt. 1 Bioassay of serotonin using rat fundus strip by three point bioassay
1. ExperimentNo. 1
Bioassay of serotonin using rat fundus strip
by three point bioassay.
Mr. Vishal Balakrushna Jadhav
Assistant Professor (Pharmacology)
GES’s Sir Dr. M. S. Gosavi COPER, Nashik-5
2. Overview of Discussion
• Objective
• Principle
• Requirements
• Experimental specifications (conditions)
• Preparation of serotonin stock solution
• Preparation of Krebs solution (PSS)
• Procedure
• Kymographrecording of contractions
• Observation table
• Calculation
• Result and interpretation
2
3. Objective
To determine unknown concentration of serotonin
(5- hydroxytryptamine, 5-HT) using rat fundus strip
by three point bioassay.
3
4. Threepoint bioassay
4
In such a type of multiple point bioassay, two responses of
standard drug (S1 and S2) and one response due to test
sample (T) are taken into consideration. The selection of
two responses of standard should be such that they lie on
the linear portion of the concentration response curve and
also the ratio between the doses should be 1:2. The test
response should be intermediate between two responses
of standard drug.
The precision, reliability and reproducibility of this bioassay are
very high. It is most commonly used for estimating the
concentration of the unknown sample.
Principle
5. 5
Rat fundus is very sensitive tissue for the study of the action
of several naturally occuring substances like 5-
hydroxytryptamine, histamine, ACh and bradykinin. Unlike
the intestinal smooth muscle (ileum), fundus preparation is
slow contracting and slow relaxing type of tissue. It is
generally employed for the bioassay of serotonin. The
fundus (the upper part of the stomach) is grey in colour and
therefore, easily identified from the pylorus (pink in colour).
A zig-zag preparation of fundus strip is prepared so as to
exposed maximum portion of the tissue to drug.
The tissue is sensitive to 1 ng/ml of serotonin, 0.05 ng/ml of
histamine and 0.2-0.5 ng/ml of ACh.
6. 6
Mechanism of contraction
Serotonin acts on 5HT2B- subtype of serotonergic receptor
which function through Gq protein and activate membrane
bound phospholipase C (PLc) → generating inositol
trisphosphate (IP3) and diacylglycerol (DAG), the secondary
messengers release Ca2+ intracellularly → cause
depolarization and thereby contraction of gastric fundus.
Overnight fasted rats are used to record better response of
serotonin on isolated rat gastric fundus .
7. Requirements
Animal: Albino rats (150-200 g, overnight fasted)
Physiological solution: Krebs solution.
Drug- Serotonin (Stock solution: 10 mcg/ml)
Chemical- Fixing solution.
Instruments: Sherrington recording drum , Student organ
bath, Aerator, Insulin or tuberculin syringe to inject drugs
in small fractions, Dissecting board and various dissecting
instruments. Frontal writing lever and stand, Pipette,
Stop watch etc.
Miscellaneous: Kymograph paper, plasticin, clips, and
thread.
7
8. Experimentalspecifications(conditions)
Isolated tissue- Isolated rat fundus strip preparation
Drug- Serotonin (Stock solution: 10 mcg/ml)
Physiological salt solution (PSS)- Krebs solution
Time cycle- Total- 5 minutes, Base line- 30 seconds, Contact
time- 90 seconds, Washing period- 3 minutes
Applied load/ tension- 1 g
Bath capacity- 40 ml
Bath temperature- 37°C
Speed of rotation of drum- 0.25 mm/ second
Magnification value (Mf) = d (F-W)/ d (F-T)
Aeration- Normal air (1- 2 bubbles/ second)
8
10. Preparationof Krebs solution (PSS)
Prepare 1 litre of Krebs solution by dissolving NaCl (6.9
g), KCl (0.35 g), MgSO4.7H2O (0.28 g), NaHCO3 (2.1 g),
KH2PO4 (0.16 g) and glucose (2.0 g) in distilled water.
MgSO4.7H2O should be added at last.
CaCl2 (0.28 g) should be dissolved separately in distilled
water to avoid chances of precipitation of salt.
Mix CaCl2 solution to the higher volume of PSS.
10
11. Procedure
Sacrifice the rat by a blow on the head and carotid bleeding.
Cut open the abdomen and expose the stomach.
Identify the fundus of the stomach (upper greyish part). Incise it
from the junction of pyloric part (pink in colour) and put it in the
dish containing Krebs solution.
Incise the fundus from the lesser curvature and open it
longitudinally. Give alternate zig-zag cuts to make a fundal strip
preparation. Tie both the ends with the thread and mount in the
organ bath containing Krebs solution at 37°C. Aerate the tissue.
Apply 1 g load and allow the preparation to equilibrate for 30
min. Using frontal writing lever with 10-12 magnification, record
the contractions due to increase concentrations of serotonin.
Since the muscle contracts slowly and relaxes slowly, a contact
time of 90 seconds and 5 min time cycle are followed to record
DRCs of stock and test solutions of serotonin.
11
12. Select two doses of standard form the DRC of stock solution of
serotonin eliciting sub-maximal responses and bearing a dose
ratio 1:2 preferentially and designate them as n1 and n2 elicits the
responses S1 and S2 respectively .
Select one dose of test from the DRC of test solution in such a
way that the response due to this dose lies preferentially
between n1 and n2 and designate it as t elicits the response T.
Record 3 sets of responses due to n1, n2 and t adding them to
organ bath in a randomized fashion as per Latin square design
mentioned below. The Latin square design of addition of doses is
followed to ensure good randomization and to account for the
fluctuating sensitivity of the tissue.
12
n1 n2 t
n2 t n1
t n1 n2
Latin Square Design -Three point bioassay
13. Measure the height of various responses to calculate the mean
height of each response (Mean of S1, S2, and T)
Calculate the unknown concentration of serotonin (T) using the
following formula and interpret the result.
Where, n1 = Lower standard dose
n2 = Higher standard dose
t = Test dose
S1 = Mean of responses of n1
S2 = Mean of responses of n2
T = Mean of responses of t
Cs= concentration of serotonin stock (10 μg/ml)
13
16. Kymograph recording of contractions- continue
16
Part III- Recording of responses of n1, n2 & t in three point bioassay.
n1- Lower standard dose (0.4ml)
n2- Higherstandard dose (0.8 ml)
t- Test dose (0.6ml)
n1 n2 t
n2 t n1
t n1 n2
Latin Square Design
Three point bioassay
18. Observation table- Example
18
Sr.
No.
Drug
(Conc.)
Dose
(ml)
Response in
Height
(cm/mm)
1 n1 0.4
2 n2 0.8
3 t 0.6
4 n2 0.8
5 t 0.6
6 n1 0.4
7 t 0.6
8 n1 0.4
9 n2 0.8
Part II- Dose Response Curve (DRC) of Serotonin (T)
(In the way as that of Serotonin stock )
Part III- Recording of responses of n1, n2 & t in three point bioassay.
19. Calculation
19
Magnificationvalue (Mf) = d (F-W)/ d (F-T)
Where-
d (F-W) → distance between fulcrumand stylus (writing tip)
d (F-T) → distance between fulcrumand point of attachment of tissue
Unknown concentration of Serotonin T (μg/ml)
Calculate mean of heightsof standard and test responses due to n1, n2 and t
Unknown concentrationof Serotonin T (μg/ml)
20. Result and interpretation
The concentration response curves (CRCs) or dose response
curves (DRCs) of serotonin stock (10 μg/ml) and test solution
using isolated rat fundus strip preparation was recorded and
interpreted graphically as sigmoid or S-shape curves.
The unknown concentration of serotonin (T) using isolated
rat fundus strip preparation by three point bioassay was
found to be .........μg/ml.
The magnification value was found to be-.........
20