Oxidative stress is a metabolic process causing imbalance between prooxidants and antioxidants favouring abundance and overbearing effects of the prooxidants. When it occurs, there is disruption in redox signals, biochemical pathways, and control leading to impaired systemic functions. It is an implicated biological mechanism behind pathological development and diseases conditions including reproductive inefficiency, cardiovascular diseases, aging, alcohol-related diseases, adult respiratory distress, atherosclerosis, and inborn error of metabolism. In animal production poor reproductive performance currently, threaten livestock productivity. Several efforts toward overcoming this challenge (poor reproductive performance) have culminated to identification of oxidative stress as the culprit because animals’ productivity is impaired either directly or indirectly by oxidative stress. In this paper, we reviewed oxidative stress as a biochemical process affecting reproduction and also hypothesized that oxidative stress is a nexus between functional biochemical process and improved productivity. Therefore, the study concluded that the abatement of oxidative stress is possible by exploring natural sources of antioxidants for the promotion of productivity through optimized reproductive index.
E-screen assay validation: evaluation of estrogenic activity by MCF7 cell cul...Agriculture Journal IJOEAR
— Natural and synthetic estrogens have been detected in rivers, lakes and estuaries in many parts of the world. Primary sources of these compounds are domestic and industrial effluents, which are not deleted after the water treatment. Estrogen has been the endocrine disruptor most researched to be very active biologically and be the etiologic agent of diverse types of cancer and other conditions such as endometriosis, precocious puberty, feminization, masculinization, sterility. In this context, we use water of 36 natural reservoirs or dams, in a bioassay to characterize their estrogenicity in culture of MCF7 cells and obtained high concentration of estrogen in samples taken in Ibiúna and Equestrian Santo Amaro / SP. However, certain concentration in our samples for most water samples from different regions was very close to the limit of quantification by bioassay and estrogen was in fmol. It has been shown that e-screen assay with MCF7 cells is a sensitive and stable tool for quantitative analysis of estrogenicity of water and can easily be developed and implemented for routine for estrogen quantification also in animal food and man, aqueous and plastics etc. Keywords— endocrine disrupters, estrogen, breast cancer cells, (MCF7) bioassay: E-screen assay
Examining Neurobehavioral Toxicity of Patulin in Adult ZebrafishQuang Nguyen
The content of this PowerPoint is strictly for the purpose of submission to the Sigma Xi Research Showcase. Please do not quote/cite/reference materials in this file in its entirety. I am not responsible for any misrepresentation of its reproduction. Any reproduction must have the author's written approval.
Otherwise, I hope you enjoy the slides.
Check out my page at http://patulinzebrafish.tumblr.com/
Some chemicals, both natural and man-made, can interfere with the hormonal system. They are called 'endocrine disruptors’. The most controversial issue is whether low level exposures to such chemicals can have adverse effects. Have endocrine disruptors affected wild life and our hormonal system? How much do we know so far?
What are Endocrine-disrupting chemicals (EDCs)?
What products contain endocrine disruptors?
How do endocrine disruptors work?(its Mechanisms of Action).
How are people exposed to endocrine disruptors?
Endocrine disrupting chemicals and their heath effects.
Pesticides:( DDT),human health consequences of exposure to DDT,and its scientific evidence and examples.
Steps to reduce exposure to endocrine disruptors
E-screen assay validation: evaluation of estrogenic activity by MCF7 cell cul...Agriculture Journal IJOEAR
— Natural and synthetic estrogens have been detected in rivers, lakes and estuaries in many parts of the world. Primary sources of these compounds are domestic and industrial effluents, which are not deleted after the water treatment. Estrogen has been the endocrine disruptor most researched to be very active biologically and be the etiologic agent of diverse types of cancer and other conditions such as endometriosis, precocious puberty, feminization, masculinization, sterility. In this context, we use water of 36 natural reservoirs or dams, in a bioassay to characterize their estrogenicity in culture of MCF7 cells and obtained high concentration of estrogen in samples taken in Ibiúna and Equestrian Santo Amaro / SP. However, certain concentration in our samples for most water samples from different regions was very close to the limit of quantification by bioassay and estrogen was in fmol. It has been shown that e-screen assay with MCF7 cells is a sensitive and stable tool for quantitative analysis of estrogenicity of water and can easily be developed and implemented for routine for estrogen quantification also in animal food and man, aqueous and plastics etc. Keywords— endocrine disrupters, estrogen, breast cancer cells, (MCF7) bioassay: E-screen assay
Examining Neurobehavioral Toxicity of Patulin in Adult ZebrafishQuang Nguyen
The content of this PowerPoint is strictly for the purpose of submission to the Sigma Xi Research Showcase. Please do not quote/cite/reference materials in this file in its entirety. I am not responsible for any misrepresentation of its reproduction. Any reproduction must have the author's written approval.
Otherwise, I hope you enjoy the slides.
Check out my page at http://patulinzebrafish.tumblr.com/
Some chemicals, both natural and man-made, can interfere with the hormonal system. They are called 'endocrine disruptors’. The most controversial issue is whether low level exposures to such chemicals can have adverse effects. Have endocrine disruptors affected wild life and our hormonal system? How much do we know so far?
What are Endocrine-disrupting chemicals (EDCs)?
What products contain endocrine disruptors?
How do endocrine disruptors work?(its Mechanisms of Action).
How are people exposed to endocrine disruptors?
Endocrine disrupting chemicals and their heath effects.
Pesticides:( DDT),human health consequences of exposure to DDT,and its scientific evidence and examples.
Steps to reduce exposure to endocrine disruptors
Aging is the progressive accumulation of damage to an organism over time leading to disease and death. Aging research has been very intensive in the last years aiming at characterizing the Pathophysiology of aging and finding possibilities to fight age-related diseases. Various theories of aging have been proposed. In the last years advanced glycation end products (AGEs) have received particular attention in this context. AGEs are formed in high amounts in diabetes but also in the physiological organism during aging. Higher levels of diabetic complications are due to poor glycemic control. The incidence and prevalence of diabetes mellitus is rising. About 50% of people with diabetes mellitus are unaware of their condition. Pharmacotherapy and Therapeutic lifestyle change (Diet, Regular exercises, Sunshine, Vitamin D and Calcium normal levels) should be the cornerstone of diabetes management.
Presentation 27 June - 1 July 2016. Asaba, Nigeria. Workshop organised by the PAEPARD supported consortium: Knowledge transfer towards cost–effective poultry feeds production from processed cassava products to improve the productivity of small-scale farmers in Nigeria.
An Introduction to the Health Effects of Endocrine Disrupting Chemicals (EDCs)
by @toxipedia
* Toxipedia website;
http://www.toxipedia.org/display/toxipedia/Endocrine+Disruptors
* Endocrine Disruptors: Sexy Stuff:
http://desdaughter.wordpress.com/2012/12/16/endocrine-disruptors-sexy-stuff/
* All our posts about Endocrine Disruptors:
http://desdaughter.wordpress.com/tag/endocrine-disruptors/
Intermittent fasting had a strong anti inflammatory effect beside the many other benefits. Intermittent fasting is an eating pattern and Interventional strategy where in individuals are subjected to varying periods of fasting. It doesn’t specify which foods you should eat but rather when you should eat them. Intermittent fasting (IF) is an eating pattern that cycles between periods of fasting and eating. It’s currently very popular in the health and fitness community. Recently attracted attention because:
1- Its Evidence-Based Health Benefits
2- Its potential for correcting metabolic Abnormalities
3- Better adherence than other methods
Biological diversity, or biodiversity, is the scientific term for the variety and variability of life on Earth. Biodiversity is the key indicator of the health of an ecosystem. Every living thing, including man, is involved in these complex networks of interdependent relationships, which are called ecosystems.
Like all healthy ecosystems, Richness of microbiota species characterizes the GI microbiome in healthy individuals. Conversely, a loss in species diversity is a common finding in several disease states. Microbiota Biodiversity helps us : 1- Combat aggressions from other microorganisms, 2- Maintaining the wholeness of the intestinal mucosa. 3- Plays an important role in the immune system, 4- Performing a barrier effect.5- A healthy and balanced gut microbiota is key to ensuring proper digestive functioning. A gut out of balance means a body out of balance which means illness including Inflammation, Allergies, Infections, Nutrient deficiencies, Weight Gain, Asthma-allergies – Autoimmunity
• Arthritis, Metabolic Bone disease, Skin problems e.g. eczema, rosacia, Mood disorders - Cognitive decline-Alzheimers and Cancer.
Endocrine Disruptors: Child healths
Vichit Supornsilchai, MD, PhD
Endocrine Unit, Department of Pediatric,
Faculty of Medicine,
King Chulalongkorn Memorial Hospital
Epigenetics, the microbiome and the environmentfathi neana
An epigenome consists of a record of the chemical changes to the DNA and histone proteins of an organism. These changes can be passed down to an organism's offspring via transgenerational epigenetic inheritance. Epigenetics, Gut microbiome and the Environment interplay like a vicious triad.
1- The epigenome is highly sensitive to external environment
2- The epigenome is highly sensitive to internal environment (Microbiome)
3- The microbiome (internal environment) is affected by the external environment
Care of the microbiome seems to be a personal issue but as it is affected by the external environment the issue must be global and a worldwide campaign have to be started.
Nutrients present in Nutrease powder play an important role in maintaining the normal functions of the human body. The major nutrients present in Nutrease powder include Natural carbohydrates, proteins, lipids, vitamins, and minerals. Besides these, there are some bioactive food components known as “phytonutrients” that play an important role in human health. They have tremendous impact on the health care system and may provide medical health benefits including the prevention and/or treatment of disease and various physiological disorders such as Andropause or Male menopause. Phytonutrients play a positive role by maintaining and modulating immune function to prevent specific diseases. Being natural products, they hold a great promise in clinical therapy. Phytonutrients in Nutrease powder are the plant nutrients with specific biological activities that support human health. Some of the important bioactive phytonutrients in Nutrease powder include polyphenols, terpenoids, resveratrol, flavonoids, isoflavonoids, carotenoids, limonoids, glucosinolates, phytoestrogens, phytosterols, anthocyanins, and probiotics. They play specific pharmacological effects in human health such as anti-microbial, anti-oxidants, anti-inflammatory, anti-allergic, anti-spasmodic, anti-cancer, anti-aging, hepatoprotective, hypolipidemic, neuroprotective, hypotensive, diabetes, osteoporosis, CNS stimulant, analgesic, protection from UVB-induced carcinogenesis, immuno-modulator, and carminative. This article reviews the current available scientific literature regarding the effect of Nutrease powder as an effective supplementation in Male & Female Fertility.
Aging is the progressive accumulation of damage to an organism over time leading to disease and death. Aging research has been very intensive in the last years aiming at characterizing the Pathophysiology of aging and finding possibilities to fight age-related diseases. Various theories of aging have been proposed. In the last years advanced glycation end products (AGEs) have received particular attention in this context. AGEs are formed in high amounts in diabetes but also in the physiological organism during aging. Higher levels of diabetic complications are due to poor glycemic control. The incidence and prevalence of diabetes mellitus is rising. About 50% of people with diabetes mellitus are unaware of their condition. Pharmacotherapy and Therapeutic lifestyle change (Diet, Regular exercises, Sunshine, Vitamin D and Calcium normal levels) should be the cornerstone of diabetes management.
Presentation 27 June - 1 July 2016. Asaba, Nigeria. Workshop organised by the PAEPARD supported consortium: Knowledge transfer towards cost–effective poultry feeds production from processed cassava products to improve the productivity of small-scale farmers in Nigeria.
An Introduction to the Health Effects of Endocrine Disrupting Chemicals (EDCs)
by @toxipedia
* Toxipedia website;
http://www.toxipedia.org/display/toxipedia/Endocrine+Disruptors
* Endocrine Disruptors: Sexy Stuff:
http://desdaughter.wordpress.com/2012/12/16/endocrine-disruptors-sexy-stuff/
* All our posts about Endocrine Disruptors:
http://desdaughter.wordpress.com/tag/endocrine-disruptors/
Intermittent fasting had a strong anti inflammatory effect beside the many other benefits. Intermittent fasting is an eating pattern and Interventional strategy where in individuals are subjected to varying periods of fasting. It doesn’t specify which foods you should eat but rather when you should eat them. Intermittent fasting (IF) is an eating pattern that cycles between periods of fasting and eating. It’s currently very popular in the health and fitness community. Recently attracted attention because:
1- Its Evidence-Based Health Benefits
2- Its potential for correcting metabolic Abnormalities
3- Better adherence than other methods
Biological diversity, or biodiversity, is the scientific term for the variety and variability of life on Earth. Biodiversity is the key indicator of the health of an ecosystem. Every living thing, including man, is involved in these complex networks of interdependent relationships, which are called ecosystems.
Like all healthy ecosystems, Richness of microbiota species characterizes the GI microbiome in healthy individuals. Conversely, a loss in species diversity is a common finding in several disease states. Microbiota Biodiversity helps us : 1- Combat aggressions from other microorganisms, 2- Maintaining the wholeness of the intestinal mucosa. 3- Plays an important role in the immune system, 4- Performing a barrier effect.5- A healthy and balanced gut microbiota is key to ensuring proper digestive functioning. A gut out of balance means a body out of balance which means illness including Inflammation, Allergies, Infections, Nutrient deficiencies, Weight Gain, Asthma-allergies – Autoimmunity
• Arthritis, Metabolic Bone disease, Skin problems e.g. eczema, rosacia, Mood disorders - Cognitive decline-Alzheimers and Cancer.
Endocrine Disruptors: Child healths
Vichit Supornsilchai, MD, PhD
Endocrine Unit, Department of Pediatric,
Faculty of Medicine,
King Chulalongkorn Memorial Hospital
Epigenetics, the microbiome and the environmentfathi neana
An epigenome consists of a record of the chemical changes to the DNA and histone proteins of an organism. These changes can be passed down to an organism's offspring via transgenerational epigenetic inheritance. Epigenetics, Gut microbiome and the Environment interplay like a vicious triad.
1- The epigenome is highly sensitive to external environment
2- The epigenome is highly sensitive to internal environment (Microbiome)
3- The microbiome (internal environment) is affected by the external environment
Care of the microbiome seems to be a personal issue but as it is affected by the external environment the issue must be global and a worldwide campaign have to be started.
Nutrients present in Nutrease powder play an important role in maintaining the normal functions of the human body. The major nutrients present in Nutrease powder include Natural carbohydrates, proteins, lipids, vitamins, and minerals. Besides these, there are some bioactive food components known as “phytonutrients” that play an important role in human health. They have tremendous impact on the health care system and may provide medical health benefits including the prevention and/or treatment of disease and various physiological disorders such as Andropause or Male menopause. Phytonutrients play a positive role by maintaining and modulating immune function to prevent specific diseases. Being natural products, they hold a great promise in clinical therapy. Phytonutrients in Nutrease powder are the plant nutrients with specific biological activities that support human health. Some of the important bioactive phytonutrients in Nutrease powder include polyphenols, terpenoids, resveratrol, flavonoids, isoflavonoids, carotenoids, limonoids, glucosinolates, phytoestrogens, phytosterols, anthocyanins, and probiotics. They play specific pharmacological effects in human health such as anti-microbial, anti-oxidants, anti-inflammatory, anti-allergic, anti-spasmodic, anti-cancer, anti-aging, hepatoprotective, hypolipidemic, neuroprotective, hypotensive, diabetes, osteoporosis, CNS stimulant, analgesic, protection from UVB-induced carcinogenesis, immuno-modulator, and carminative. This article reviews the current available scientific literature regarding the effect of Nutrease powder as an effective supplementation in Male & Female Fertility.
The biology of Aging in Insects From Drosphila to other insects and back.pptxArchana Ramanji
This particular presentation describes aging in insects and explains the mechanisms underlying it, particularly in Drosophila including eusocial insects.
Who this is for: Health professionals.
Description: Dr. Sheila Bushkin-Bedient will be speaking on the prenatal origins of disease and why we should be focusing on studying possible connections between unconventional natural gas extraction and diabetes, obesity, and cancer.
About the Speaker: Sheila Bushkin is a member of the Institute of Health and the Environment at the State University at Albany, and Concerned Health Professionals of New York. She has been a member of the Medical Society of the State of New York for 15 years. Her specific areas of interest involve environmental health issues, chronic diseases, health concerns of older adults, and CME for physicians.
Plant Evolution
Plant Distribution Essay
Essay on Plant hormones
Plant Products Essay
Invasive Plants Essay
Ecology And Plant Ecology
Plant And Animal Cells Essay
Essay about Power Plant
Essay about Plant Cell
The Tea Plant Essay
Plant Reproduction Essay
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.
Antioxidant potentials of tannic acid on lipid peroxidation induced by severa...Premier Publishers
Various prospective studies have indicated the antioxidant potency of tannic acid in several models. However, there is no clear-cut evidence revealing that the reported antioxidant properties of tannic acid remains potent regardless of the lipid sources and pro-oxidants employed for the oxidative assault. Hence, this study sought to investigate the antioxidant properties of tannic acid against cerebral and hepatic lipid peroxidation induced by several pro-oxidants (Iron (II) sulfate, Sodium nitroprusside, cyclophosphamide and acetaminophen) in vitro. Rats were decapitated under mild ether anesthesia and the tissues were rapidly dissected, placed on ice, weighed and immediately homogenized in cold 50 mM Tris-HCl, pH 7.4 (1/10, w/v). The homogenates were centrifuged for 10 min at 4000 g to yield a pellet that was discarded and a low-speed supernatant (S1). Our results indicated that Fe (II) showed the highest pro-oxidative effects in both tissues lipids. Furthermore, tannic acid demonstrated potent inhibitory effects against lipid peroxidation in both tissues lipids regardless of the pro-oxidant employed. To this end, there is a dire need to exploit the protective benefits of tannic acid as a potential exogenous antioxidant against lipid peroxidation with a view to providing solution to the global oxidative stress menace.
The role of antioxidant supplement in immune system, neoplastic, and neurodegenerative disorders: a point of view for an assessment
of the risk/benefit profile. Nutritional Journal
Gene–environment interactions in development and disease lovely - 2016 - wi...BARRY STANLEY 2 fasd
ETHANOL: THE EMPEROR OF ALL TERATOGENS
An account of gene/environment interaction in relation to the petro chemical age, and the historical significance of alcohol as teratogen.
Protective effect of garlic extract against maternal and fetal cerebellar dam...Prof. Hesham N. Mustafa
Background: In spite of its industrial usefulness and varied daily uses, lead (Pb) pollution is a widespread ecological problem that faces the humans in the 21th century. Pb was found to produces a wide range of toxic effects including neurotoxicity especially to the developing and young offspring. Recently, the utilisation of herbal plants has received a significant attention where there has been rising awareness in their therapeutic use; among these is the garlic. In light of the above, the current study is designed experimentally in female pregnant rats in order to investigate the beneficial role of garlic extract in the protection from the maternal and foetal cerebellar damage produced by administration of different doses of Pb during pregnancy.
Materials and methods: Positively pregnant female rats were divided into five groups; one control group, two Pb-treated groups (exposed to 160 and 320 mg/kg b.w. of Pb, respectively) and two groups treated with both Pb and garlic (exposed to Pb as previous groups together with 250 mg/kg b.w./day of garlic extract). Treatments started from day 1 to day 20 of pregnancy, where the mother rats of different experimental groups were sacrificed to obtain the foetuses. Pb level in the maternal and foetal blood and cerebellum was estimated by spectrophotometry. Specimens of the cerebellum of different mother and foetal groups were processed to histological and immunohistochemical staining for microscopic examination.
Results: The results showed that administration of Pb to pregnant rats resulted in a dose-dependent toxicity for both mothers and foetuses in the form of decrease in maternal weight gain, placental and foetal weights, brain weight and diminished foetal growth parameters, which were prominent in rat's group treated with larger dose of Pb. In Pb-treated rats, Pb level in blood and cerebellum was high when compared with the control group. The histopathological examination of the cerebellum of treated dams and foetuses showed marked alterations mainly in the form of Purkinje cell degeneration and lack of development of foetal cerebellum. Co-treatment of garlic extract along with Pb resulted in a significant decrease in Pb levels as compared with those treated with Pb alone with improvement of the histopathological changes.
Conclusions: This study was useful in evaluating the hazardous effects of uncontrolled use of Pb in general and in assessing the developmental and neurotoxicity of foetuses due to exposure during pregnancy in particular. Co-administration of garlic has beneficial effects in amelioration of Pb-induced neurotoxicity and reversing the histopathological changes of the cerebellum of mother rats and foetuses. (Folia Morphol 2018; 77, 1: 1-15).
Keywords: Purkinje cells; garlic; glial fibrillary acidic protein; lead.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
2. Sikiru, et al.: Oxidative Stress and Reproductive Ineffciencies: The Science, Evidences, and Solutions
AEXTJ/Jan-Mar-2018/Vol 2/Issue 1 18
against foreign agents at low concentrations.
However, they become harmful and cause disease
conditions when there is overproduction which
reduces natural body antioxidants capacity. As
part of the immune system complex, prooxidants
use their reactive capacities to inhibit pathogenic
organisms’ activities hence keeping diseases
development at bay – this is the mechanism of
action of macrophage cells in the neutrophils. At
cellular level, prooxidants over production alter
roles of genetic functionalities by destruction of
purines and pyrimidine bases causing genetic
mutation and errors of genetic expression.[3]
Reproduction is the most important system ensuring
procreation of living organisms. In both animals
and humans, reproduction ensures giving birth to
offspring and increasing population. Reproduction
apart from leading to parturition holds the key to
increase population and productivity of animal
species. Through increased population and
productivity, supply of animal products for human
consumption, and industrial uses are sustained.
Reproduction generally can be regarded as set of
activitiesinsequentialorderleadingtotheemergence
of new young offspring of a given species of living
organism and increase existence of living organisms
under different environmental conditions.[33]
Reproduction has both biological and economic
benefits in livestock production enterprises
because it brings forth younger offspring of an
animal species for production of consumable and
industrial animal products as well as conservation
of the animal species. In promotion of reproduction
in animals; reproductive performance including
conception rates, gestation length, sexual
receptivity, litter size, parturition loss or survival,
weaning weight, litter weight, litter growth rates,
and milk production capacity are economic
parameters that are continuously on improvement
in animal production enterprises. In rabbits
production, for example, they are very important
parameters for reproductive performance
evaluation because they determine profitability,
products yield and are major factors determining
enterprise profitability and production objectives.
FUNCTIONAL BIOCHEMICAL
PROCESS OF OXIDATIVE STRESS
Oxidative stress can be traced back to oxygen
which is one of the most valuable elements for
survival of living organisms (aerobic) because it
is the main element for respiration. It is, however,
paradoxical that the same oxygen could be toxic
through production of its derivatives known
as prooxidants which includes ozone-oxygen,
singlet oxygen, superoxide, and hydrogen
peroxide. Formation of all these oxygen
derivatives changes oxygen an element hitherto
known to be beneficial, to become hazardous and
damaging to body systems. Although this does
not occur under normal body functioning; in
any situation of shift in balance of prooxidants
and antioxidant defense, either by exogenous or
internal factors leading to overproduction of the
prooxidants, it results into a condition known as
oxidative stress – condition which is causal, or at
least an ancillary factor in the pathology of many
diseases.[37]
Biologically, oxygen is sourced as a waste product
from the process of photosynthesis in green
plants by absorption of energy from sunlight for
production of plant foods. This is the process that
guarantees availability of oxygen for respiration
in humans and animals. Oxygen is the only
element with the most appropriate physical state,
satisfactory solubility in water and with desirable
combination of kinetic and thermodynamic
properties to serve as biological oxidant.
However, there is high cost associated with the
use of oxygen as a biological oxidant which is its
reduction to formation of prooxidants and other
biological damaging species. These prooxidants
and other damaging species are responsible for
damage at both cellular and systemic levels in both
humans and animals because they cause oxidative
damage.[29]
Oxidative damage is associated with energy
demanding activities whereby prooxidants
because of their strong reactivity pick up useful
biomolecules in the animals system thereby
causing damage and unhealthy state. Other
high energy demanding activities such as
thermoregulation also cause oxidative damages
but to a lesser extent compared with level of
damages associated with energy demanding
activities of reproduction. For instance,
studies have revealed that oxidative damage
in lactating animals was higher than oxidative
damage for thermoregulation by non-breeding
animals which is also an energy focus activity
in animals. Since it is not economical and even
hardly feasible to raise animals without them
reproducing, overcoming challenges of oxidative
3. Sikiru, et al.: Oxidative Stress and Reproductive Ineffciencies: The Science, Evidences, and Solutions
AEXTJ/Jan-Mar-2018/Vol 2/Issue 1 19
damages associated with reproduction is highly
desirable.[14,27,45]
Oxidative balance as presented in Figure 1
when triggered cause damages in reproductive
activities in animals and human being. Major
problems of reproductive performance associated
with oxidative stress damage are infertility in
female human subjects while in male subjects;
it reduces fertilizing capabilities of sperm.[9]
All
damages associated with oxidative stress can
be summarized to inhibit production of primary
and accessory reproductive cells, reproductive
organs dysfunction, inhibition of embryonic
development, and growth. Unexplained infertility
in human female is associated with oxidative
stress based on reports from studies revealing
higher concentration of reactive oxygen species
(ROS) and malondialdehyde conjugated diene
but lower antioxidants concentration in peritoneal
fluids.[32,42]
Oxidative stress because of its roles as an important
functional biochemical process involved in
reproduction got serious attention of researchers
who conducted several research on oxidative
stress effects on reproduction; summary of some
of the works is as summarized in Table 1.
PHYSIOLOGICAL SIGNIFICANCE
OF OXIDATIVE STRESS ON
REPRODUCTIVE PERFORMANCE
Production of ROS trigger imbalance between
antioxidants and prooxidants which cause high
damages in reproductive activities not in animals
alone but also in human being. Major problems
of reproductive performance including infertility
in female human subjects; reduced fertilizing
capabilities of sperm in male have been linked
with negative effects of oxidative stress. All
damages associated with oxidative stress can be
summarized to inhibit production of primary and
accessory reproductive cells, reproductive organs
functionalities, embryonic development, and
growth. Unexplained infertility in human female
participants has been linked to oxidative stress
based on reports from studies which revealed
higher concentration of ROS concentration in
and malondialdehyde conjugated diene but lower
antioxidants peritoneal fluids.
ROS cause oxidative stress which has strong
relevancy in reproductive inefficiencies in both
humans and animals. In male organisms, it
affect both seminal qualities and reproductive
functions of the spermatozoa while in female
organisms, it affect suitability of reproductive
environment for oocytes maturation and
development of embryo. As ROS increases more
than the natural balance of antioxidants body
system naturally increase effort to overcome
this situation and this has been reported to lead
to aging because of damages inflicted on vital
biological components which naturally supports
and promote structural integrity of the body.
This is an indication again that reproduction is a
trade-off for aging.[33]
Oxidative stress occasioned by elevated levels of
free radicals negatively impact on performance
of animals reproduction, especially during
gestation and lactation periods. Workers stated
that the impact is as a result of increased need
for nutrients for the growing fetus, and growth
and development of mammary structures for
milk production which all require high nutrients
that is usually limited due to poor intake
by animals during these periods. Oxidative
stress physiologically reduces the secretions
of gonadotropin-releasing hormones by the
hypothalamus and subsequently limiting the flow
of luteinizing and follicle-stimulating hormones
by the pituitary and sex steroids by the gonads.
This physiological failures cause reduced fertility
rates and retardation of embryo growth.[9]
Figure 1: Oxidative stress balance[46]
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Table 1: Some literature evidence on oxidative stress effects on reproduction
Title Summary Authors
Birds sacrifice oxidative protection for reproduction ROS are unavoidable by-products of oxidative metabolism and
these by-products through damages on DNA, lipids and cellular
proteins and cause aging
[33]
Constraint and cost of oxidative stress on reproduction:
Correlative evidence in laboratory mice and review of the
literature
Evolutionary ecology pointed that current and residual
reproductive values are negatively linked by the so-called
cost of reproduction among which oxidative stress is the most
prominent
[7]
Good genes, oxidative stress, and condition-dependent sexual
signals
Oxidative stress through generation of free radicals modulate
reproductive immunity
[41]
Oxidants, antioxidants, and the degenerative diseases of aging Oxidative metabolism is essential part of life but it has
expensive trade-off causing damages to DNA, protein, and lipid.
It is a major cause of aging and degenerative diseases
[15]
Oxidative stress and its implications in female infertility – a
clinician’s perspective
ROS have a role in the modulation of gamete quality and gamete
interaction
[9]
Oxidative stress and its role in female infertility and assisted
reproduction: Clinical implications
ROS are involved in physiological functions and act as
mediators in
various signaling processes
[36]
Oxidative stress and living cells ROS are onslaught causing molecular damages in cells of plants,
animals, and man
[20]
Oxidative stress as a mediator of life history trade-offs:
Mechanisms, measurements, and interpretation
Oxidative stress is underlying mechanism for understanding the
concept of life-history. Managing oxidative stress is likely to be
a major determinant of life histories, as virtually all activities
generate ROS
[30]
Increased susceptibility to oxidative stress as a proximate cost of
reproduction
Investment in current reproduction is usually paid regarding
reduced future reproduction and increased mortality;
reproduction decreases antioxidant defenses, illustrating that
oxidative stress represents a cost of reproduction
[16]
Oxidative stress in an ART setting Increase in levels of ROS without a concomitant rise in
antioxidant defenses leads to oxidative stress causing cellular
damages on lipid, protein, and DNA which are all associated
with suboptimal ART success rates
[12]
Oxidative stress, antioxidants, and animal function Reactive oxygen metabolites generated during body metabolism
can enter into reactions that, when left uncontrolled, can impair
the performance of dairy cows thereby reducing productivity. To
optimize performance, oxidative stress in high producing cows
must be controlled by supplying all known antioxidant nutrients
and by minimizing effects of substance or condition stimulation
generations of ROS
[28]
Oxidative stress, prooxidants, and antioxidants: The interplay Oxidative stress can be viewed as an imbalance between
prooxidants and antioxidants in the body. It is an important
biochemical process in body growth and development; genomic
evidence showed oxidative stress relationship with disease
development, incidence of malignancies and autoimmune
disorders, increased susceptibility to bacterial, viral, and
parasitic diseases
[8]
Oxidative damage increases with reproductive energy
expenditure and is reduced by food-supplementation
Life-history theory identified the process of reproduction to
negatively affect survival. Costs of reproduction are thought to
be physiologically based, but the underlying mechanisms remain
poorly understood; meanwhile oxidative stress is implicated as
the underlying mechanism
[34]
Role of oxidative stress and antioxidant supplementation in
pregnancy disorders
Oxidative stress is widely implicated in failed reproductive
performance, including infertility, miscarriage, diabetes-related
congenital malformations, and preeclampsia
[25]
The effects of oxidative stress on female reproduction: A review Oxidative stress adversely affects sperm quality, oocytes
production, and functions. Environmental effects of pollution
can trigger oxidative stress while antioxidant supplementation
may be effective in controlling generation of ROS for improved
reproduction
[10]
Fecundity and survival in relation to resistance to oxidative
stress in a free-living bird
Resistance to oxidative stress is a major determinant for high
reproductive output regarding survival to breeding season and
size of egg clutches in free-living birds in the wild
[31]
ROS: Reactive oxygen species, ART: Assisted reproductive techniques
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GENETIC EVIDENCE OF OXIDATIVE
STRESS EFFECTS ON REPRODUCTIVE
PERFORMANCE
During oxidative stress condition, there is usually
elevated levels of ROS, and this is reported to
cause genomic instability through manipulation
of deoxyribonucleic acid coding, and it also
reduce activities of protective antioxidants on
cells, thereby resulting into cellular destruction
and or damage.[2]
According to[44]
there is cellular
destruction and damage on DNA, protein, and
lipids molecules as a result of oxidative stress.
Also[5,13]
reported that there are dysfunctions of
structures in spermatozoa of luekocytospermic
patients; they justified this observation on
overproduction of prooxidants which originally
are meant for building defense against infections
during spermatogenesis.
High levels of ROS are also reported to induce
oxidative damage of DNA in sperm plasma
membrane, mitochondrion, and nuclear genome.
The danger in this damage is not associated
with poor reproductive performance alone
but is capable of causing cancer and inherited
infertility in the off-springs.[4]
This damage impact
negatively on life and survival of the spermatozoa,
inability of sperm to fertilize and destruction of
oocytes in case there was fertilization. Oxidative
stress is also implicated in DNA fragmentation - a
condition common in subfertile human beings.
This fragmentation causes mutation in offspring
because the fragmentation is hardly repaired
ahead of fertilization. According to the study of[14]
oxidative stress affect reproductive performance
through damage in different cellular components
and biomolecules at all levels of reproduction
from fertilization, to maintenance of pregnancy
and lactation period in both humans and animals;
some of these biomolecules are cellular genetic
materials.
As ROS increases in the body of animal models
and human subject more than the natural balance
of antioxidants, body system naturally increases
effort to overcome this situation, and this has
been reported to lead to aging because of damages
inflicted on vital biological components which
naturally supports and promote structural integrity
of the body.This damages include damage on DNA
by destruction of purine and pyrimidine bases,
destruction of protein, and lipids biomolecules
through fast reactions between the reactive
species and the biomolecules such as proteins,
carbohydrates, and lipids. This is an indication
again that reproduction is a trade-off for aging.
To inhibit aging as result of reproduction, there
is need for reducing rates of oxidative stress in
food-producing animals such as for sustainable
production of meat that is safer for human
consumption and public health friendliness.
INFLUENCE OF OXIDATIVE STRESS
ON LIVESTOCK PRODUCTIVITY
Suboptimal livestock productivity is closely
linked to oxidative stress because oxidative stress
promotes a decrease in residual reproductive
value of animals with age advancement. In
livestock production, optimal productivity is a
complex of reproductive efforts as it involves
animal physiological maturity or aged before the
animal can be productive.[33]
In dairy production
for example, cows’ ability to produce the
right quantity and quality of milk depend on
reproduction because the animal is required to
get fertilized and calve before it can produce milk
during lactation. Similarly, in poultry production,
hen day production capacity of laying birds
depends on reproductive maturity of the birds. In
rabbit and pig production, frequency and number
of off-spring which are major factors determining
productivity depends on reproductive maturity
and optimum performance of animals.
All the indicators listed above require huge
biological investment from animals for them to
be productive. However, as animals attempt to
invest for future reproductive output; oxidative
stress presents itself as a limiting factor because
it mediates between reproductive output and
resources allocation for reproduction, especially
energy. Animal species based on resources
allocation for reproduction are categorized to
be short live with high reproductive rates and
long live with low reproductive rates. This
phenomenon is termed livelihood trade-off for
reproduction. Studies supporting this hypothesis
was demonstrated to be scientific through the work
of[33]
in Zebra finches whereby increased oxidative
stress was reported with increase brood size and
vice-versa. Oxidative stress will definitely occur
as animals increase effort to reproduce because
since reproduction is an energy focus activity;
continuous demand for increasing energy for
metabolic activities will result into development
of oxidative stress through accumulation of
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non-reduced oxygen from cellular oxidative
phosphorylation.
In actively producing animals, oxidative stress
limits reproductive and productive output
through reproductive failures. An example of
reproductive failure causing loss due to oxidative
stress is impaired uterine contractibility. Uterine
contraction which is an important process for
embryo implantation in animals when impaired,
inhibit the process of parturition because the
normal process of uterine smooth muscle
contraction becomes hampered. In ewe and cows,
impaired uterine contraction was reported to
decrease transportation of sperm for fertilization
process, and in a condition of poor sperm
transportation, conception rates decrease is a
common condition.
ANTIOXIDANT TREATMENT EFFECTS
ON OXIDATIVE STRESS
In both animals and humans, there is an internal
systemofantioxidantsdefensewhichputoxidative
stress under check at all times. This system is
composed of bioactive compounds internally
produced by animals or man in vivo or supplied
externally to the animals. These antioxidants
are categorized broadly as enzymatic or non-
enzymatic antioxidants. Enzymatic antioxidants
include catalase (CAT), superoxide dismutase
(SOD), and glutathione peroxidase (GPx)
while nonenzymatic include malondialdehyde,
glutathione, tocopherol, astaxanthin, carotenoids,
fucoxanthin, and vitamins. Antioxidants either
enzymatic or nonenzymatic can be from natural or
synthetic sources and can be applied to suppress
activities of ROS in natural reproductive or
assisted reproductive activities.[17,21,39]
Exogenous antioxidant supplementation is a
common practice for fertility treatment in man
and it improves reproductive performance. In
male human fertility patients; using of Vitamin
E was reported for improvement of post-thaw
sperm motility and improvement of DNA
integrity. Also[40]
reported the use of Vitamin
E infertility patient and in cryopreservation
of sperm of male fertility patients.[19]
Reported
Vitamin C for reduction of malondialdehyde
level – an indication of a reduction in sperm
lipids peroxidation, reduction of DNA structural
damage, and improved sperm progressive
motility as well as viability of sperm all resulting
into improved fertility in human fertility patients.
These researchers concluded that exogenous
supplementation of antioxidants is a proven
means of reducing oxidative stress associated
with reproductive performance and the need to
discover different sources of antioxidants capable
of improving reproductive performance.
In their study reported potency of micronutrients
supplementation for control of oxidative stress in
female human subjects as highly successful.[26]
The study concluded that additional treatment
with micronutrients, starting 3 months before
in vitro fertilization cycles, protects the follicular
microenvironment from oxidative stress, thus
increasing the number of good quality oocytes
recovered at the pickup. Common micronutrients
reported as antioxidants include selenium,
manganese, and zinc.
While exploring phytochemical sources for
antioxidants capable of reducing oxidative stress,
reported that Telfairia occidentalis aqueous
extract ameliorates oxidative stress in brain of
rats exposed to gamma rays.[2]
The study reported
better levels of both enzymatic and nonenzymatic
antioxidants in the brain of rats treated with the
aqueous extracts after different durations of
exposure to gamma rays (source of oxidative
stress).
Turmeric powder for preservation of rabbit
meat; the turmeric powder proved to be a strong
antioxidant source because it prevented lipids
peroxidation in rabbit meat even more than
synthetic antioxidant source-like ascorbic acid
used in the preservation of rabbit meat. This
work contributed turmeric as a natural source
of antioxidants in widely consume products
such as meat burger as alternative for synthetic
preservatives which have in them potential toxic
compounds.[47]
Supplementation of pomegranate
peel in rabbit diet is a source of natural antioxidant
and concluded that it improved reproductive
performance of rabbit due to protective action
of antioxidants against lipid oxidation in the
cell membrane of the pomegranate.[24,45]
The
studies recommended using pomegranate peel
for newborns, which exhibit a greater sensitivity
to oxidative damage than adults, and for the
development of the immune system in young
animals.
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MICROALGAE AS SOURCE OF DIETS
AND ANTIOXIDANTS FOR LIVESTOCK
PRODUCTION
Exploration of natural antioxidant sources against
oxidativedamagesinanimalscanserveaslivestock
production system to beat because it is capable of
raising animal’s productivity above suboptimal
levels, especially at critical physiological states
of pre-pubertal, gestation and lactation periods.
Natural antioxidants in animal nutrition will
be playing similar roles antioxidant-rich foods
including onions, garlic, tomatoes, pepper, and
orange are playing in human nutrition. In human
nutrition, these foods in addition to their regular
supply of nutrients are playing roles of protection
against microbial damage and supply of bioactive
compound preventing oxidative damages in
human body system.
Microalgae including Chlorella vulgaris,
Dunaliella, and Spirulina have been explored for
production of functional bioactive compounds
including vitamins, carotenoids, phycocyanin,
astaxanthin, and other photosynthetic pigments.
Inclusion of microalgae at 10% showed positive
effects on growth performance and feed utilization
efficiency in broiler chickens. Inclusion of algae
at 2.5% and 10% resulted in positive effects on
growth performance by increasing weight gain,
nutrient utilization, feed digestibility, carcass
quality, physiological functions, and intestinal
microbial environment.[48]
Chemical composition of microalgae can be
efficiently use for improvement of animal
product quality and can as well partially replace
conventional dietary protein sources in animal
diets because in a study, researchers replaced
soybean at 0%, 20%, 40%, 60%, and 80% with
C. vulgaris for production of diets fed to broilers
chicken. It was proved that inclusion of the algae
as a replacement for soybean is feasible since
it contains approximately 60% crude protein
with balanced amino acids as well as vitamins,
minerals, and antioxidants. Inclusion at 20% in
the study was reported tolerable by the chicken
and improved the chicken performance.[49]
In a similar study, C. vulgaris a microalgae
was also explored as a source of nonchemical
antibiotics and growth promoter; the researchers
reported that the algae is an alternative antibiotic
source and growth promoter in chicken because
it promoted production performance, immune
system, and intestinal microflora of the chicken. It
was also reported that even at very small quantity,
C. vulgaris significantly influence performance
and improved physiological activities in chicken
because it increases weight gain and phagocytic
activity of white blood cells as well as lymphatic
tissue development.[22,23]
Influence of selenium-
enriched with C. vulgaris on performance of
broiler chickens was also investigated, and the
study showed positive effect of the preparations
(0.3 mg of Chlorella enriched selenium per Kg
of diets) on body weight gain and activity of
antioxidant enzyme GPx.[18]
In addition, inclusion
of C. vulgaris at 0.07%, 0.14%, and 0.21% in
Broiler chickens diets from starter till 42 days
improved the performance of the chicken through
better feed conversion ratio and better immune
system.[35]
C. vulgaris inclusion at 1.25% of
laying birds diets positively affected oxidative
stability of the egg yolk lipids and stored eggs
this was evaluated by replacement of microalgae
with fishmeal, it was concluded that microalgae
have potential replacement capability due to its
nutritional compositions.[38]
OXIDATIVE STRESS BIOMARKERS OF
PHYSIOLOGICAL REPRODUCTIVE
SIGNIFICANCE
Cells contain a large number of antioxidants that
naturally prevent or repair the damage caused
by prooxidants and also regulate redox-sensitive
signaling pathways; these antioxidant enzymes
include SODs, CAT, and GPx. Shortfall of these
internal body antioxidant systems are an indication
of oxidative stress and this occurs time-to-time in
animals hence feeding antioxidant-rich feedstuff
such as algae can contribute to improve internal
antioxidant system in animals whereby all these
enzymes will be available at optimum levels.
As biological markers, poor or low activities of
these antioxidant enzymes can serve as indicators
of levels of oxidative stress damage at different
reproductive stages.
SODs are metalloenzymes that catalyze the
dismutation of superoxide anion to molecular
oxygen and hydrogen peroxide which is a
strong mechanism for development of cellular
antioxidant in the body. Poor or low activities of
SODs results into increased oxidative stress. SOD
work in complementation with another antioxidant
enzyme called CAT. SOD converts superoxide
radicals by dismutation into hydrogen peroxide
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while CAT convert hydrogen peroxide into water
in order to become harmless in the body. This is
the mechanisms of action of SODs. SODs type
depend on associated metals either Manganese
SOD or Copper-Zinc SOD. Manganese SOD is
located in the mitochondria while Copper-Zinc
SOD is located in the cytoplasm.[48]
CAT is antioxidant enzymes which convert
hydrogen peroxide – a very strong oxidative
damagingcompoundintowaterwhichisaharmless
compound in the body. Activities of CAT enzyme
are largely located in subcellular organelles called
peroxisome in the cell. Measuring of the CAT
can be done using CAT ELISA kit where CAT
activity is measured by a spectrophotometric
procedure evaluating peroxide removal.[49]
GPx
is an enzyme that catalyzes the reaction which
reduces peroxides such as hydrogen peroxides
to protect cell from oxidative damage. GPx is an
enzyme that catalyzes the reaction which reduces
peroxides such as hydrogen peroxides to protect
cell from oxidative damage. Higher concentration
of this antioxidant can reduce oxidative stress
effects in animals.
Mitochondria respiration at cellular level is a
process of oxidative phosphorylation leading
to the production of by-products which are free
radicals that cause cellular damages. Natural
process of cellular protection against these free
radicals was identified as physiological roles
of uncoupling proteins (UCPs) family. It can,
therefore, be hypothesized that UCPs are potential
part of cellular antioxidant defense system
because of its inhibition of oxidative damage on
mitochondrion as well as elevated levels of ROS
due to its absence. UCPs are group of molecular
compounds associated with protection of
mitochondria during the process of thermogenesis.
They uncouple respiration (oxidation) from
adenosine triphosphate (ATP) synthesis in
mitochondria. In a situation whereby oxidation is
uncoupled from production of energy in the form
of ATP; generation of ROS is inhibited in the cell
and hence these proteins (UCPs) can, therefore, be
categorized as part of cellular antioxidant defense
system. These proteins are categorized as UCPs
1, 2 and 3; they are anion carrier proteins found
in the mitochondria and distributed in cells of
different parts of the body including spleen, lungs,
stomach, and white adipose tissue.[50]
Mitochondria as the focal point for cellular
thermogenesis and heat dissipation exerts its
metabolic responsibilities through passive
exchange of small metabolites between
cytoplasm and the intermembrane space with
the aid of transporter proteins known as UCPs.
Structurally their amino acids is hundred and
contain two domains of transmembrane while
their molecular weight is between 28 KDa and
34 KDa.[51]
Members of this protein family
include UCPs 1, 2, 3, 4, and 5 and are generally
anioncarriers.UCP-1isassociatedwithmetabolic
protection against cold stress. It is found in the
brown adipose tissue which is responsible for
mediation of internal heat generation for survival
of external cold environments. Another pointer
to roles of these protein in energy generation
and balance is the effects of thyroid hormone,
leptin and noradrenalin hormone on its levels.
These hormones are all involved in the process
of energy generations and balance, and they also
increase levels of UCP-1; therefore, it can be
deduced that the protein is an important carrier
in mitochondria physiological role of energy
production and dissipation.[51]
CONCLUSION
Oxidative stress is a biochemical process that it
naturally inevitable for normal body functions;
however, it can turn-out to become a biochemical
process negatively affecting reproduction hence
it can be hypothesized that oxidative stress is a
nexus between functional biochemical process
and improved productivity. Therefore, the study
concluded that abatement of oxidative stress is
highly important for promotion of reproduction
and dietary supplementation of microalgae can
serve as natural sources of antioxidants for
reducing negative effects of oxidative stress.
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