This study investigates the effect of Bacillus licheniformis protease on the growth performance and the nutrient utilization of broilers. This reduces dietary requirements and environmental impact of excretion whilst increasing energy utilisation and growth in broilers. Join the LinkedIn discussion on Feed Enzymes: http://www.linkedin.com/groups?home=&gid=4738175&trk=anet_ug_hm&goback=%25 Learn more about DSM Animal Nutrition at: http://www.dsm.com/markets/anh/en_US/home.html Or why not follow us on Twitter: @DSMFeedTweet

1. Supplementation of a monocomponent Bacillus licheniformis
protease improved growth
performance and selectively
improved nutrient digestibility in
broilers
1Olukosi,
1ASRC,
O. A., 1Beeson, L. A., 2Pirgozliev, V. and 3Smith, A.
SRUC, Ayr, UK
2Harper Adams University College, Newport, UK
3DSM Nutritional Products, Heanor Gate, UK

2. Contents
•
•
•
•
Introduction
Study
Materials and Methods
Results
– Growth performance
– Nutrient utilisation
• Summary and Conclusion
2

3. Outline
• Introduction
• Study
• Materials and Methods
• Results
– Growth performance
– Nutrient utilisation
• Summary and Conclusion
3

4. Proteases
Proteases: enzymes responsible for
the breakdown of proteins into its
constituent peptides and amino acids
4

5. Introduction
Exogenous
protease
Improve
energy
utilisation
Reduce
anti-nutritional
factors
Reduce
endogenous
losses
5

6. Anti-nutritional factors
E.g. protease/ trypsin inhibitors, lectins
•
•
•
•
•
Digestibility
Endogenous N losses
Gut irritation = Mucus production
Bacterial fermentation
Initiation of immune response
6

7. Endogenous losses
• Nitrogen
• Dietary Crude Protein
• Endogenous enzymes
Endogenous proteases
• Mucus
7

8. Energy utilisation
Proteases may help improve energy utilisation:
Reduced energy expenditure on unnecessary
processes
– Enzyme synthesis
– Mucus production
– Immune response
Energy for muscle/ protein accretion = GROWTH
8

9. Benefits of exogenous proteases
•
N/ AA digestibility
•
Energy utilisation
• Complementation of endogenous enzyme
activity
•
Dietary CP and energy requirements
•
N excretion
9

10. Outline
• Introduction
• Study
• Materials and Methods
• Results
– Growth performance
– Nutrient utilisation
• Summary and
Conclusion
10

11. Objective and Hypothesis
Objective: To investigate the effects of a protease on
broiler growth performance and nutrient utilisation
Hypothesis: Exogenous protease
reduction in dietary CP level
will
enable
11

12. Enzyme characteristics
• Mono-component protease
• Fermentation of a sporulation-deficient Bacillus
licheniformis strain
• Expresses synthetic gene encoding serine protease
(EC3.4.21.-)
12

13. Outline
• Introduction
• Study
• Materials and Methods
• Results
– Growth performance
– Nutrient utilisation
• Summary and Conclusion
13

14. Experimental design
• 1116 one-day-old male broilers
• Randomised Complete Block Design
• Three treatments
• 12 replicate pens, 31 birds per pen
14

15. Diets
3 wheat-SBM dietary treatments
– (PC) adequate CP diet
– (NC) 97% of CP requirements
– NC + protease (15,000 PROT/kg)
Background phytase (2000FYT/kg) and xylanase
(200FXU/kg)
3-phase feeding-programme:
Starter 0-13d; Grower 13-35d; Finisher 35-42d
15

16. Analyses
• Ileal digesta collection on d21
• Digesta and diet analysed for GE, N, AA, Ti
• Data analyses using SAS
– Generalised Linear Model
– Tukey’s test
– Significance at P ≤ 0.05
16

17. Outline
• Introduction
• Study
• Materials and Methods
• Results
–Growth performance
–Nutrient utilisation
• Summary and Conclusion
17

18. Growth performance – Starter, d 0-13
Weight gain (g)
1.228
400
a
1.224
b
b
FCR
Gain (g)
395
390
FCR
385
380
1.220
1.216
375
1.212
370
1.208
PC
NC
Protease
PC
NC
Protease
Protease increased weight gain during starter phase
18

19. Growth performance – Grower, d 13-35
FCR
Weight gain (g)
1.700
1.680
2200
FCR
Gain (g)
2250
2150
2100
1.660
1.640
1.620
2050
PC
NC
Protease
1.600
PC
NC
Protease
Protease during grower phase matches PC performance
19

20. Energy and Nitrogen
N digestibility (%)
IDE (MJ/kg)
80
15.6
a
a
15.2
78
ab
14.8
b
b
b
76
14.4
74
14.0
13.6
72
PC
NC
Protease
PC
NC
Protease
Protease improves IDE & N digestibility (P < 0.05)
IDE – Ileal Digestible Energy
20

21. Ileal digestibility
66
80
64
78
62
76
60
58
74
Dry matter, %
68
a
64
60
a
Lysine, %
PC
NC
Protease
b
56
52
Protease increased Cys
digestibility (P < 0.01)
48
Cysteine, %
21

22. Outline
•
•
•
•
Introduction
Study
Materials and Methods
Results
– Growth performance
– Nutrient utilisation
• Summary and Conclusion
22

23. Summary and Implications
•
Dietary CP requirements
•
Energy utilisation for growth
•
Growth efficiency
•
N excretion – Environmental benefits
23

24. Conclusion
The serine mono-component enzyme tested:
• Is effective at promoting weight gain
and nutrient digestibility during
the starter phase
• Improved energy and
N digestibility
24

25. Acknowledgements
DSM Nutritional Products
Basel, Switzerland
25