ENZYME BENEFIT: • ENVIRONMENTAL IMPACT (N and P) • COST REDUCTION / PERFORMANCE INCREASE • REDUCTION OF WET LITTER COMPLAINTS • INCREASED FEED INTAKE (PHYTASE) • MORE CONSISTENT PERFORMANCE • FORMULATION FLEXIBILITY

1. GETTING THE MOST OUT OF YOUR DIETS:
OPTIMAL FORMULATION WITH DSM ENZYMES
BRETT ROOSENDAAL
20 NOVEMBER 2013

3. RAPID ADOPTION

4. OBJECTIVEOBJECTIVE
Ileal Protein
Dig.Coeff.
AME (MJ/kg DM) Starch Dig.Coeff.
CONTROL 0.78bc 14.07e 0.75c
XYLANASE 0.77c 14.37d 0.83b
PHYTASE 0.81ab 14.62c 0.86ab
PROTEASE 0.82a 14.81bc 0.83b
XYL + PHY 0.81abc 14.75c 0.89ab
XYL + PROT 0.80abc 14.66c 0.86ab
PHY + PROT 0.83a 14.99ab 0.88a
XYL + PROT + PHY 0.81ab 15.18a 0.87a
SEM 0.01 0.06 0.01
Sultan, A., Li, X., Zhang, D., Cadogan, D.J. and Bryden, W.L. (2010) Dietary enzymes alter sorghum
Protein digestibilityand AME content. APSS 21
Sultan, A., Gan, C.Y., Li, X., Zhang, D. and Bryden, W.L. (2011) Dietary enzyme combinations improve
sorghum ileal protein and starch digestibilityduring the broiler starter phase. APSS 22

5. PHILOSOPHY ON ENZYMESPHILOSOPHY ON ENZYMES
• IMPROVE DIGESTION OF UNDIGESTED SUBSTRATES
• ENZYMES ARE CHOSEN ON THE BASIS OF SUBSTRATES
• IMPROVED BIRD PERFORMANCE (ADG, FCR, MORTS)
THROUGH MAXIMISING NUTRIENT UTILIZATION
• IMPROVED PROFITABILITY

6. EXPECTATION
• SUPERDOSING OF PHYTASE?
• CORN-SOYA DIETS – 85 TO 95% OF STARCH, PROTEIN
AND LIPIDS ARE DIGESTED
• POORLY DIGESTED INGREDIENTS AS LOW AS 75%
• UNREALISTIC TO EXPECT ENZYMES TO ACHIEVE 100%
• REALISTIC TO EXPECT 25-35% OF UNDIG. FRACTION

7. THE BIRD
pH Ret. Min %
CROP 5.7 10 to 50 17.5
PROV 1.5
30 to 90 32GIZZ 2.7
PDUO 4.6
5 to 10 3.5DDUO 6
JEJEN 6.3 20 to 30 11
ILEUM 6.7 50 to 70 25
CECA 8 20 to 30 11

8. THE ENZYMES
ENZYME TARGET SUBSTRATE TARGET FEEDSTUFF
PHYTASES PHYTIC ACID ALL PLANT INGREDIENTS
B-GLUCANASES B-GLUCAN BARLEY, OATS, RYE
XYLANASES ARABINOXYLANS WHEAT, BARLEY, RYE,
TRITICALE, FIBROUS
PLANT MATERIALS
PROTEASES PROTEINS ALL PROTEIN
INGREDIENTS
AMYLASE STARCH CEREAL GRAINS, GRAIN
LEGUMES
MANNANASES MANNANS SOYABEAN, PALM
KERNAL

9. PHYTASE
Van Der Klis, J.D. and Star, L. (2012) Efficacy of different Phytase Products on Productivity
And Bone Ash in Broilers. Schothorst Feed Research Report No. 1186

10. PHYTASE
Van Der Klis, J.D. and Star, L. (2012) Efficacy of different Phytase Products on Productivity
And Bone Ash in Broilers. Schothorst Feed Research Report No. 1186

11. THE PHYTASE SUBSTRATE

12. PHYTASE
Walk, C.L., Bedford, M.R., Santos, T.S., Paiva, D., Bradley, J.R., Wladecki, H., Honaker, C. and
McElroy, A.P. (2013) Extra-phosphoriceffects of superdoses of a novel microbialphytase.
Poult. Sci. 92:719-725

13. PROTEASE
Phase 2 Feed
Nutritional Level in the Feed AA Ratios RONOZYME ProAct Contribution Contribution of RONOZYME ProAct in Feed
CP (%) 22 3.311 CP (%) 0.728
Lys Dig. (%) 1.290 2.823 Lys Dig. (%) 0.036
Met Dig. (%) 0.593 46% 3.749 Met Dig. (%) 0.022
M+C Dig. (%) 0.903 70% 5.424 M+C Dig. (%) 0.049
Thr Dig. (%) 0.826 64% 7.244 Thr Dig. (%) 0.060
Trp Dig. (%) 0.219 17% 1.680 Trp Dig. (%) 0.004
Arg Dig. (%) 1.445 112% 3.034 Arg Dig. (%) 0.044
Val Dig. (%) 0.993 77% 3.561 Val Dig. (%) 0.035
Leu Dig. (%) 1.213 94% 1.143 Leu Dig. (%) 0.014
Ile Dig. (%) 0.864 67% 2.921 Ile Dig. (%) 0.025
His Dig. (%) 0.477 37% 3.630 His Dig. (%) 0.017
G+S Dig. (%) 1.896 147% 3.836 G+S (%) 0.073
RONOZYME ProAct Dose RONOZYME ProAct Matrix Value
200 CP (%) 3,642
Lys Dig. (%) 182.084
Safety Margin to be consider (x*SD) Met Dig. (%) 111.237
0 M+C Dig. (%) 244.894
Thr Dig. (%) 299.037
Trp Dig. (%) 18.421
Arg Dig. (%) 219.201
Val Dig. (%) 176.834
Leu Dig. (%) 69.308
Ile Dig. (%) 126.227
His Dig. (%) 86.639
G+S (%) 363.684

14. THE PROTEASE SUBSTRATE

15. PROTEASE

16. PROTEASE

17. COMBINATION ENZYMES
• MULTIPLE COMPLEXES TARGETING THE SAME
SUBSTRATE NOT BENEFICIAL
• XYLANASE + PHYTASE
• IMPROVE PHOSPHORUS UTILIZATION THROUGH
INCREASING CELL WALL PERMEABILITY
(Coppedge,J.R., Klein, J., Brown, B., Ratliff, B., Ruch, F. and Lee, J.T. (2011) Effects of co-administration of phytaseand NSPase on broiler
performanceand bone ash. Int. J. Poult.Sci. 10:12:933-939)
• EXTENDED GASTRIC RESIDENCE TIME – PHYTASE
POTENTIATING (WHEN NEEDED) AND IMPROVING
GASTRIC PROTEOLYSIS (WHEN NEEDED)
(Kalmendal, R. and Tauson,R. (2012) Effects of a xylanaseand protease,individually or in combination,and an ionophorecoccidiostat on
performance,nutrient utilization, and intestinal morphology in broiler chickensfed a wheat-soyabean-meal based diet. Poult. Sci. 91:1387-1393)
(Mike Bedford, personal communication,3/10/2013)

18. THE NSP SUBSTRATE

19. VARIABILITY IN RESPONSE
0
20
40
60
80
100
120
0,25 0,275 0,3 0,325 0,35 0,375 0,4 0,425 0,45 0,475 0,5
Retainable Phosphorus %
OPTIMAL PHYTASE TEST SITUATION
Response
PCNC
CALCULATED
DEFICIENCY = 0.1%
ACTUAL
DEFICIENCY = 0.1%
VARIABLE + UN-PREDICTABLE
• ENZYME
• SOURCE
• DOSE RATE
• SIDE ACTIVITIES
• DIET
• INGREDIENT QUALITY
• DIET COMPOSITION
• FORM
• PARTICLE SIZE
• DENSITY
• BIRD
• AGE
• SEX
• BREED

20. COMMERCIAL RESULTS
0,00
0,50
1,00
1,50
2,00
2,50
0 5 10 15 20 25 30 35 40
Live weight
Age
Rainbow KZN (Cycle 264-285)
Cobb Standard
B19 Trial 2011-014 (Cobb)
University Trial 2011-005 (Cobb)

21. COMMERCIAL TRIALS

22. COMMERCIAL TRIALS

23. COMMERCIAL TRIALS

24. COMMERCIAL TRIALS

25. METEN WETEN

26. ENZYME BENEFIT
• ENVIRONMENTAL IMPACT (N and P)
• COST REDUCTION / PERFORMANCE INCREASE
• REDUCTION OF WET LITTER COMPLAINTS
• INCREASED FEED INTAKE (PHYTASE)
• MORE CONSISTENT PERFORMANCE
• FORMULATION FLEXIBILITY

27. ENZYME BENEFIT

28. CONCLUDING REMARKS
• ACTIVE AREA OF RESEARCH AND DEVELOPMENT
• ROI
• ENZYME BENEFIT WELL RECOGNISED
• REALISTIC EXPECTATIONS
• STRATEGY IS TO INCREASE DIGESTION OF
UNDIGESTED SUBSTRATES