This study investigates the effect of Bacillus licheniformis protease on the growth performance and the nutrient utilization of broilers. This reduces dietary requirements and environmental impact of excretion whilst increasing energy utilisation and growth in broilers.
Join the LinkedIn discussion on Feed Enzymes:
http://www.linkedin.com/groups?home=&gid=4738175&trk=anet_ug_hm&goback=%25
Learn more about DSM Animal Nutrition at: http://www.dsm.com/markets/anh/en_US/home.html
Or why not follow us on Twitter: @DSMFeedTweet
This study investigates the effect of Bacillus licheniformis protease on the growth performance and the nutrient utilization of broilers. This reduces dietary requirements and environmental impact of excretion whilst increasing energy utilisation and growth in broilers.
Join the LinkedIn discussion on Feed Enzymes:
http://www.linkedin.com/groups?home=&gid=4738175&trk=anet_ug_hm&goback=%25
Learn more about DSM Animal Nutrition at: http://www.dsm.com/markets/anh/en_US/home.html
Or why not follow us on Twitter: @DSMFeedTweet
We are a strong India based Animal health care company established in year 1999 and well known for innovative and quality products. Our capabilities include manufacturing nutritional supplements in dosage forms like gels, powders, drenches and suspensions. Our product range include vitamins, minerals, direct fed microbials, enzyme blends, immune-globulins, amino acids and electrolytes with packaging options ranging from few ounces to 30lts. We make products for dairy, beef, sheep, goats, poultry, horses, dogs and cats.
Our quality system is ISO:9001:2008 certified and followed strict cGMP.
I encourage you to visit our website at www.vetcoindia.com and learn more about our company and products.
Some enzymes work against anti-nutritional factors (ANF), which are produced by plants, in order to obtain more nutrients from plants for increased animal nutrition.
The correct enzyme (or combination of enzymes) need to be used for the specific target. This depends on the plant cell wall structure. For example, the cell walls of the endosperm of corn is comprised mainly of insloluble arabinoxylans, which means that only xylanases are able to degrade this wall to increase the energy value of corn based diets.
Read the presentation to find out more about the activity of different types of enzymes working alone or together.
>Since the ban of antibiotic growth promoters (AGPs) in the European Union, the industry has been looking for valid alternatives to improve health, immune status and performance in animal agriculture.
>The industry focuses on developing solutions that mimic the effects of AGPs. However, the exact mode of action of the AGPs to improve poultry performance is not fully understood.
Most AGPs alternatives try to prevent the proliferation of pathogenic bacteria and to modulate indigenous bacteria to improve immune status and performance.
>As an industry, we have made limited progress in improving gut health. Maybe we need to change the approach to find the most optimal solution.
>As part of a stable ecological system, pathogens per se do not represent a threat to animal health. The dynamic nature of the gastrointestinal microflora in chickens makes maintaining the right balance in the microbial ecosystem.
>Despite a large amount of scientific work has been done on the topic, a lack of consistent improvement has been registered.
>To improve gut health more consistently, a broader approach, involving a combination of nutrition, feed technology and husbandry management needs to be taken.
>In addition, gut-health is too complex to be solved from
only one point of view. Input should be obtained from different disciplines, including food technology, human nutrition, veterinary and human medical sciences.
>DSM will start an holistic program to come to tailor made solutions to improve the gut ecosystem with the objective of reducing the use of antibiotics in the poultry industry.
We are a strong India based Animal health care company established in year 1999 and well known for innovative and quality products. Our capabilities include manufacturing nutritional supplements in dosage forms like gels, powders, drenches and suspensions. Our product range include vitamins, minerals, direct fed microbials, enzyme blends, immune-globulins, amino acids and electrolytes with packaging options ranging from few ounces to 30lts. We make products for dairy, beef, sheep, goats, poultry, horses, dogs and cats.
Our quality system is ISO:9001:2008 certified and followed strict cGMP.
I encourage you to visit our website at www.vetcoindia.com and learn more about our company and products.
Some enzymes work against anti-nutritional factors (ANF), which are produced by plants, in order to obtain more nutrients from plants for increased animal nutrition.
The correct enzyme (or combination of enzymes) need to be used for the specific target. This depends on the plant cell wall structure. For example, the cell walls of the endosperm of corn is comprised mainly of insloluble arabinoxylans, which means that only xylanases are able to degrade this wall to increase the energy value of corn based diets.
Read the presentation to find out more about the activity of different types of enzymes working alone or together.
>Since the ban of antibiotic growth promoters (AGPs) in the European Union, the industry has been looking for valid alternatives to improve health, immune status and performance in animal agriculture.
>The industry focuses on developing solutions that mimic the effects of AGPs. However, the exact mode of action of the AGPs to improve poultry performance is not fully understood.
Most AGPs alternatives try to prevent the proliferation of pathogenic bacteria and to modulate indigenous bacteria to improve immune status and performance.
>As an industry, we have made limited progress in improving gut health. Maybe we need to change the approach to find the most optimal solution.
>As part of a stable ecological system, pathogens per se do not represent a threat to animal health. The dynamic nature of the gastrointestinal microflora in chickens makes maintaining the right balance in the microbial ecosystem.
>Despite a large amount of scientific work has been done on the topic, a lack of consistent improvement has been registered.
>To improve gut health more consistently, a broader approach, involving a combination of nutrition, feed technology and husbandry management needs to be taken.
>In addition, gut-health is too complex to be solved from
only one point of view. Input should be obtained from different disciplines, including food technology, human nutrition, veterinary and human medical sciences.
>DSM will start an holistic program to come to tailor made solutions to improve the gut ecosystem with the objective of reducing the use of antibiotics in the poultry industry.
The rising global human population and the improving general human welfare standards comes with an increasing demand for animal proteins. According to an outlook report by the Organisation for Economic Co-operation and Development (OECD) and the Food and Agriculture Organisation of the United Nations (FAO), the poultry meat production will grow over the next 10 years at around 2.3 percent annually to around 134.5 million tonnes of meat making it the largest meat sector from 2020 onwards.
Dr Carlene Starck, Postdoctoral Research Fellow at Riddet Institute, New Zealand: http://www.kiwifruitsymposium.org/presentations/kiwifruit-and-digestive-comfort-in-vitro-and-in-vivo-supporting-evidence/
Presentation at the 1st International Symposium on Kiwifruit and Health.
Kiwifruit (Actinidia deliciosa) hosts a number of beneficial properties for gut health. In addition to its high fibre content, water holding capacity and levels of the vitamins C and E, its consumption has been reported to provide relief of symptoms of gastrointestinal discomfort.
Zoo-technical performances of weaner rabbits fed Nutryzyme® supplemented dietsAI Publications
The study assessed the zoo-technical performances of weaner rabbits fed Nutrizyme supplement diets. This study aimed at determining the zoo-technical performances of the weaner rabbits fed Nutrizyme supplement diets while we specifically determined the zoo-technical performances, the apparent nutrient digestibility as well as the hematological and serum Biochemical of weaner rabbits fed Nutrizyme supplement diets. Eighteen rabbits were allotted into three dietary treatments with each having three replicates in a completely randomized design format. Each replicate (unit) housed two rabbits. The Nutrizyme powder was incorporated in the diets at 0, 125, and 250 ppm respectively. The parameters appraised include average daily feed intake, feed efficiency, average final weight and the heamatological and serum profiles. All data generated were subjected to analysis of variance using statistical packages for social sciences (SSPM) packages. There were significant (p<0.05) differences in the total weight, average daily weight, average daily feed intake as well as the feed efficiency. Rabbits fed diets 3 (250mg/kg Nutrizyme inclusion) had the best result in terms of the total weight (1068g), average daily (0.25), respectively. There were significant (p<0.05) differences in the digestibility of nutrients among the rabbits as indicated in the results. The crude protein digestibility improved with increased enzyme inclusion in the diets. Rabbits fed the control diet had the least nutrient digestibility. The heamoglobin concentrations, white blood cells and serum metabolites were not significantly influenced (p<0.05) by dietary treatments. The packed cell volumes (PCV) of 36.03 to 41.06% were within the normal values of 35 to 45%, also red blood cell counts and the mean cell heamoglobin concentration (MCHC), were within the range reported for rabbits. Enzyme was found to be good nutrient metabolite that could enhance the growth of weaned rabbits. The already established quantity (125g/ton of feed) should be maintain as either increase or decrease in the internationally recommended quantity did not significantly (p<0.05) affect the zoo-technical performances nutrient digestibility, heamatological and serum metabolites of weaner rabbits.
Presentation for first doctoral seminar on Advances in poultry nutrition.pptxPallaviMali14
Due to ban on use of antibiotics, introduction of probiotics/ prebiotics
/synbiotic (combination of pro-prebiotic) / organic
acids in poultry nutrition as replacement for
antibiotics.
Conclusions
• Each additive affects microflora in a different manner
• Succesful and Sustentable Additives should contribute to mantain microflora diversity
• Some additives may also affect the host directly, not only the microbial communities
• Effects at host level should be understood and used to improve holistic efficiency
Breeding for nutritional quality in pulsesDhanuja Kumar
Legumes have been part of the human diet since the early ages of agriculture. Legumes are consumed in many forms: seedling and young leaves are eaten in salads, fresh immature pods and seeds provide a green vegetable, and dry seeds are cooked in various dishes. Legume seeds provide an exceptionally varied nutrient profile, including proteins, fibres, vitamins and minerals.
Breeding for nutritional quality entails an improvement primarily in protein quantity and quality which are of paramount significance.
PROBLEMS AND PROSPECTS OF BREEDING FOR NUTRITIONAL QUALITY
• Negative correlation between yield and protein content.
• Negative correlation between protein and sulphur containing amino acids
• Lack of proper field screening technique.
Bio fortification through Genetic EngineeringBalaji Rathod
Crop Bio-fortification is the idea of breeding crops to increase their nutritional value.
Bio-fortification differs from ordinary fortification because it focuses on making plant foods more nutritious as the plants are growing, rather than having nutrients added to the foods when they are being processed.
This is an improvement on ordinary fortification when it comes to providing nutrients for the rural poor, who rarely have access to commercially fortified foods.
Similar to Maximixing Protein Value: Impact of Enzyme on Poultry Cultivation (20)
Perpres 71/2015 ttg Penetapan dan Penyimpanan Barang Kebutuhan Pokok dan Barang Penting, Harga HPP Telur, dan harga telur 2 tahun terakhir tiap bulannya.
This presentation, created by Syed Faiz ul Hassan, explores the profound influence of media on public perception and behavior. It delves into the evolution of media from oral traditions to modern digital and social media platforms. Key topics include the role of media in information propagation, socialization, crisis awareness, globalization, and education. The presentation also examines media influence through agenda setting, propaganda, and manipulative techniques used by advertisers and marketers. Furthermore, it highlights the impact of surveillance enabled by media technologies on personal behavior and preferences. Through this comprehensive overview, the presentation aims to shed light on how media shapes collective consciousness and public opinion.
Acorn Recovery: Restore IT infra within minutesIP ServerOne
Introducing Acorn Recovery as a Service, a simple, fast, and secure managed disaster recovery (DRaaS) by IP ServerOne. A DR solution that helps restore your IT infra within minutes.
This presentation by Morris Kleiner (University of Minnesota), was made during the discussion “Competition and Regulation in Professions and Occupations” held at the Working Party No. 2 on Competition and Regulation on 10 June 2024. More papers and presentations on the topic can be found out at oe.cd/crps.
This presentation was uploaded with the author’s consent.
Have you ever wondered how search works while visiting an e-commerce site, internal website, or searching through other types of online resources? Look no further than this informative session on the ways that taxonomies help end-users navigate the internet! Hear from taxonomists and other information professionals who have first-hand experience creating and working with taxonomies that aid in navigation, search, and discovery across a range of disciplines.
0x01 - Newton's Third Law: Static vs. Dynamic AbusersOWASP Beja
f you offer a service on the web, odds are that someone will abuse it. Be it an API, a SaaS, a PaaS, or even a static website, someone somewhere will try to figure out a way to use it to their own needs. In this talk we'll compare measures that are effective against static attackers and how to battle a dynamic attacker who adapts to your counter-measures.
About the Speaker
===============
Diogo Sousa, Engineering Manager @ Canonical
An opinionated individual with an interest in cryptography and its intersection with secure software development.
Sharpen existing tools or get a new toolbox? Contemporary cluster initiatives...Orkestra
UIIN Conference, Madrid, 27-29 May 2024
James Wilson, Orkestra and Deusto Business School
Emily Wise, Lund University
Madeline Smith, The Glasgow School of Art
3. 3
Protein as 2nd most expensive component of feed
On a typical broiler feed,
protein sources are
around 30% inclusion to
meet amino acid
requirements.
Poultry ME: 3000 kcal; Dig Lys: 1.15%; *Current Ingredient prices were converted from IDR to USD
50%
30%
12%
NutrientComposition
Energy Protein Others
TraceMinerals Vitamins Amino Acid
4. 4
Based on INRA 2002 / Rhone Poulenc 1996
100%
50% 55% 60% 65% 70% 75% 80% 85% 90% 95%
industrial AA
corn
SBM 46
wheat
fish meal 70
sunflower 28 or 34
SBM 44
rape meal 34
Barley
peanut meal 49
wheat bran
MBM 55
cotton seed meal
PBPM 58
feather meal
Average Digestibility of Amino Acids
Challenge of Protein Sources in Relation to Digestibility
5. 5
What is the negative effect of
undigested protein in poultry?
7. 7
Quality of Protein Source: Many SBMs Have
High Trypsin Inhibitor Level
Traditional SBM
CDE
ABCD
B
CDE
DE
BCDE
AB
CDE
CDE
BCDE
E
ABCD
ABCDE
DE
ABCDE
BCDE
Interquartile
range (IQR)
Outlier
Outlier
Outlier
Median
Whisker:
smallest value
within 1.5*IQR
from Q1
Whisker: largest
value within
1.5*IQR from Q3
Q1 (25th %)
Q3 (75th %)
Mean
A
ABCD
ABCDE
AB
9. 9
How trypsin inhibitors in soy bean meal affect
performance of swine and poultry?
• Trypsin inhibitors; a protein that mostly found in
leguminous seeds and inhibit activity of trypsin in GIT
• Causes of hypertrophy of pancreas
• Reduces protein and amino acids digestibility (4-6%)
• Reduces energy utilization (3-4%)
• Increase mucus production (2%)
• Interfere normal gut microbiota
• Increase incidence of necrotic lesions
• Over all gut health implications
10. 10
Higher TI values
in soy products
result in lower
AA digestibility
DP100/EP150
25% inclusion of full-fat extruded soybean
varying in TI level, corn-SBM diets
Cys Thr Gly Ser Val Asp Ala Phe Pro Leu His Ile Met Glu Lys Arg
Basal
AA
AID,
%
0
66
68
70
72
74
76
78
80
82
84
86
88
TI = 4.08 mg/g in soy products
TI = 5.79 mg/g in soy products
High Trypsin Inhibitors in SBM reduce
amino acid digestibility
11. 11
Undigested Protein and Poor Quality SBM
impacts Gut Health
The gut is responsible for the digestion and absorption of nutrients.
If gut function is impaired, digestion and absorption of feed will be
reduced and bird performance and welfare will be compromised.
12. 12
What technology can be applied to
mitigate the effect of undigested
protein and poor quality SBM to
maximize protein value?
13. 13
Common Enzymes in Feed
Phytase Phytic acid
P and Ca
Anti
nutritional
factor
Energy
CP/AA
Trace minerals
NSP
Non starch
polisaccharides
Energy
Viscosity &
Complex structure
CP/AA
14. 14
Protease Enzyme: The Future Is Now
Protease
Undigested
protein/AA
CP/AA-Energy
Gut health factor
15. 15
Background on Proteases
• Industrial enzyme market is about $2.5–3 billion per year
– enzymes used in food processing ($800 million)
– enzymes for animal feed ($400 million)
– nonfood/feed enzyme applications ($1.4–1.7 billion)
• Proteases --- about 60% of the total enzyme market ($1.5–1.8 B/year)
• Alkaline Proteases: with approximately $1 billion per year (mostly use in
detergent)
Examples of Alkaline Proteases Cleavage site (C-terminal)
Chymotrypsin Aromatic residues – Tyr, Trp, Phe
Trypsin Basic residues – Lys, Arg
Comprehensive Biotechnology, 3rd edition, Volume 3, 2011
https://doi.org/10.1016/B978-0-444-64046-8.00187-7
16. 16
85%
5%
10%
Protein/AA
Digestible Cibenza DP100 Undigestible
85%
15%
Protein/AA
Digestible Undigestible
Protease Enzyme: The Future Is Now
With increasing
price of SBM, it is
important to
maximize the
value of protein
for poultry
production.
19. 19
Immature digestive system and less
secretion of digestive enzymes
Higher incidence of microbial fermentation
in hind gut which leads to increase in
pathogen production
Higher susceptibility to anti-nutritional
factors and lower nutrient digestibility
Proteases can support to address common
problems during Early Broiler Stage
20. 20
• Modify gut microbiota, prevent dysbacteriosis
– Reduce ileal Clostridium perfringens
• F. Yan, J. Dibner, C. D. Knight, M. Vazquez-Anon, N. Odetallah,
and S. Carter, 2011. Effect of dietary protein and protease
supplementation on performance and gut health of broiler chicks.
Abstract 57. PSA-AAAP Joint Annual Meeting, July 16-19, 2011,
St. Louis, Missouri. Poult. Sci. 90 (E-Suppl. 1): 19
• F. Yan, J. J. Dibner, C. D. Knight, and M. Vazquez, 2017. Effect of
carbohydrase and protease on growth performance and gut health
of young broilers fed diets containing rye, wheat, and feather
meal. Poultry Science. 96: 817-828.
http://dx.doi.org/10.3382/ps/pew300
– Increase gut microbiota species diversity
• F. Yan, J. Chen, J. Escobar, C. Foran, T. Wineman, C. Atwell,
and M. Vazquez-Anon, 2016. Effect of protease on growth
performance, intestinal morphology, gut barrier function, and gut
microbiota of broilers. Abstract 0278. The XXV World’s Poultry
Congress, September 5-9, 2016. Beijing, China
– Reduce ileum and colon E. coli
• Taiwan study
– Increase ceca lactic acid bacterial
• Taiwan study
– Decrease ceca VFA
• Taiwan study
• Improve gut morphology: ↑ V/C ratio; better villus tip
integrity
– H. Wang, Y. Guo, and J. Shih, 2008. Effects of dietary
supplementation of keratinase on growth performance, nitrogen
retention and intestinal morphology of broiler chickens fed diets with
soybean and cottonseed meals. Animal Feed Science and
Technology, 140: 376–384
– Effects of Xylanase and Protease on Gut Health and Growth
Performance of Newly Hatched Broiler Chickens. HERCHLER,
MARISSA M.S. thesis, North Carolina State University
• Improve barrier function, reduce acute phase protein
– F. Yan, J. Dibner, C. D. Knight, M. Vazquez-Anon, N. Odetallah, and
S. Carter, 2011. Effect of dietary protein and protease
supplementation on performance and gut health of broiler chicks.
Abstract 57. PSA-AAAP Joint Annual Meeting, July 16-19, 2011, St.
Louis, Missouri. Poult. Sci. 90 (E-Suppl. 1): 19
• Reduce digesta viscosity
– F. Yan Poultry Science paper above. Poultry Science.
http://dx.doi.org/10.3382/ps/pew300
– Effects of Xylanase and Protease on Gut Health and Growth Performance
of Newly Hatched Broiler Chickens. HERCHLER, MARISSA M.S. thesis,
North Carolina State University
Supporting trials for Protease Cibenza DP100
on Gut Health Benefits
21. 21
• Reduce inflammation - decrease IL-10, IL-1β
– Effect of protease and bacitracin methylene disalicylate on growth performance, gut morphology, and gut barrier function of broilers.
Abstract T162. International Poultry Scientific Forum , Atlanta Georgia January 25-26, 2016 (IL-1 β)
– B00IFY016026 (IL-10)
• Alleviate oxidative stress -reduce serum and ileum MDA and ileum protein carbonyl
– Effects of Xylanase and Protease on Gut Health and Growth Performance of Newly Hatched Broiler Chickens. HERCHLER, MARISSA M.S. thesis,
North Carolina State University
• Decrease excreta and ceca ammonia
– Taiwan study
• Aside from scientific research and studies, numerous field trials and testimonials supporting
the improvement in growth performance and uniformity of the flock with the use of protease
Cibenza DP100.
Supporting trials for Protease Cibenza DP100
on Gut Health Benefits
22. 22
The value of all enzymes is …
…To have a more profitable business
23. 23
Formulation Savings using
Protease Cibenza DP100 –
Reducing Feed Cost
• Use of matrix values (based on
improving protein digestibility
studies)
• Can maximize use of alternative
ingredients (+ 2-3% more of less
digestible ingredients)
$3.28 savings/ton of
a typical grower feed
Poultry ME: 3000 kcal; Dig Lys: 1.15%
24. 24
Unique Protease Feed Enzymes
Broad spectrum serine
protease from Bacillus
licheniformis
600,000 IU/g
protease activity
Intrinsically
heat-stable
Complements
endogenous enzymes
Provides healthy gut
for young animals
Effective in
hydrolyzing:
• Protein from both plant and
animal origin
• Anti-nutritional factors
(i.e. trypsin inhibitor)
25. Improves over-
all profitability
of the farm
Hydrolyzes
trypsin
inhibitors
Improves
gut
health
• Balances gut microbiota
– Improves gut morphology
– Reduces C. perfringens population
in the gut
– Reduces anti-inflammatory
response in birds
• Improves over-all profitability
– Formulation savings
– Flexibility in use of alternative
ingredients
Benefits of Protease CIBENZA DP100
26. 26
Message
• Poor soy bean meal quality may affect performance of poultry production.
• Undigested Protein can have negative impact on growth performance of flock.
• Technology and years of research on protease can help reduce the impact of
high ingredient prices on poultry cultivation.
• Use of Protease: #TheFutureIsNow.
