We investigated the effects of protease (Ronozyme ProAct, PRO) supplementation on the AID of 3 cereals in the presence or not of a multi-enzyme complex with β-glucanase and β-xylanase activity (GLU+XYL). The AID of most of AA was higher for corn and wheat than for barley. The inclusion of GLU+XYL increased the AID of DM, CP and of all the AA, except Met and Lys. The inclusion of PRO increased the AID of Gly and tended to increase that of most of the other AA. The combination of both enzymes did not improve further AA digestibility any.

1. Influence of protease enzyme supplementation on
apparent ileal digestibility of amino acids of corn,
wheat and barley grains
L. Cámara1,2, M. V. Kimiaeitalab1, J. D. Berrocoso1, C. E. Grewehr1,3, P. Guzmán1, C. R. Angel4, A. Smith5, and G. G. Mateos1,2
1 Departamento de Producción Agraria, U. P. Madrid, 28040 Madrid, Spain
2 CEI Campus Moncloa, UCM-UPM, 28040 Madrid, Spain
3 Current address: Universidade do Estado de Santa Catarina, Lages, Brazil
4 Department of Animal and Avian Sciences, University of Maryland, College Park, 20742, Maryland
5 DSM Nutritional Products (UK) Ltd., Heanor Gate Industrial Estate, DE75 7SG Heanor, Derbyshire, UK
2,304 one-d-
old chicks
Ross 308
4 blocks
12 treatments
24 rep (8 chicks)/trt
Main cereal
(corn vs. wheat vs. barley)
Protease
(0 vs. 200 mg/kg)
GLU+XYL
(0 vs. 100 mg/kg)
Diet
1 to 18 d of age
Commercial diet
18 to 22 d of age
Experimental diet
92% cereal 8%
NFD
AID of the AA
Day 22
MATERIALS AND METHODS
DM Thr Glu Pro Gly Ala Cys Val Met Leu Lys CP
Cereal
Corn 81.3a 66.6 86.6ab 84.5b 72.8 85.9a 76.4 76.2 85.6a 87.3a 76.5 76.4
Wheat 72.1ab 69.4 92.5a 90.9a 75.2 74.6b 81.8 76.1 82.2ab 82.3ab 73.1 79.7
Barley 71.3b 66.7 85.4b 84.3b 70.3 72.0b 76.6 74.5 78.4b 78.2b 73.7 74.1
SEM1 2.252 2.772 1.424 1.534 2.568 1.499 2.325 2.270 1.262 1.395 2.138 2.260
* NS * * NS ** NS NS * * NS NS
1 n=32 (a cage with 8 chicks)
RESULTS
Main cereal of the diet and the AID of the AA, %
60.0
65.0
70.0
75.0
80.0
85.0
90.0
DM Thr Glu Pro Gly Ala Cys Val Met Leu Lys CP
+
+
+
+
*
NS +
+
+ NS
NS
NS
0 200
60
65
70
75
80
85
90
DM Thr Glu Pro Gly Ala Cys Val Met Leu Lys CP
b
b
b
b
b
c c
b
b c
b
c
a
a
a
a
a
ab ab
a
a ab
a
ab
a
a
a a
a
a
a
a
a a
a
a
ab
a
a a
a
b
b
a
ab b
ab
b
-(GLU+XYL) -PRO -(GLU+XYL) +PRO
+(GLU+XYL) -PRO +(GLU+XYL) +PRO
P < 0.001
We investigated the effects of protease
(Ronozyme ProAct, PRO) supplementation
on the AID of 3 cereals in the presence or
not of a multi-enzyme complex with β-
glucanase and β-xylanase activity (GLU+XYL).
The AID of most of AA was higher for corn
and wheat than for barley. The inclusion of
GLU+XYL increased the AID of DM, CP and of
all the AA, except Met and Lys. The inclusion
of PRO increased the AID of Gly and tended
to increase that of most of the other AA. The
combination of both enzymes did not
improve further AA digestibility any.
CONCLUSIONS
ABSTRACT
The supplementation of the 2 enzymes in isolation showed some
benefits on AID of the AA but no further effect was seen when
these were combined. The inclusion of XYL improved the AID of
DM and of all AA and CP except Met and Lys .The combination of
both enzymes however, did not improve AA digestibility any
further. Trends observed when combining different enzyme
activities in short term digestibility trials need further study and
may not reflect the response seen combining different enzyme
combinations on animal performance studies or digestibility
studies when fed as part of a complete diet from hatch to
slaughter.
Interaction GLU+XYL x protease (mg/kg) on the AID of AA, %
Effect of protease supplementation (mg/kg) on the AID of AA, %
SEM (n=72): 0.404-1.021
SEM (n=144): 0.285-0.722

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