Selenium

2. Introduction
• High ambient temperature Heat stress in broiler chicken
• Reduces feed intake, live weight gain, feed efficiency, and immune response
Increase production of reactive oxygen species (ROS)
• Disturb the balance between oxidation and antioxidant defense systems,
resulting in lipid peroxidation and damage the proteins, DNA, and cell
membrane integrity
 Balance between ROS production and the antioxidant system must be
established to maintain immune function, health and productivity

3. • Natural antioxidants play vital roles in protecting cells from ROS by
reducing free radicals and preventing the peroxidation of lipids
• The antioxidant system includes numerous antioxidant enzymes, such
as glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and
catalase (CAT)
• Selenium (Se) is a trace mineral required for the proper functioning of
primary metabolic pathways including the thyroid hormone
metabolism, immune system and antioxidant status.
• Increasing the dietary Se levels increases the feed intake and weight
gain and improves the antioxidant status and intestinal morphology
• However, increasing the dose of inorganic Se in broiler feed increases
the chances of toxicity
• Therefore, selenium nanoparticles (SeNPs) are being explored due to
their higher bioavailability, reduced toxicity and higher surface area,
which improve catalytic activity

4. Objectives
To compare the effects of two different Se sources (nano-Se) on growth
performance, lipid peroxidation, antioxidative status, immune function
and Se retention in broilers reared under thermoneutral or high ambient
temperature conditions

5. Material Method
• Site
• Kagoshima University, Japan
• Birds
 100 day old chicks of broiler (Ross breed)
 Feed
 Day 1-14: Commercial starter diet containing 22% crude protein and 3,000 kcal/kg
 Day 15-30: Basal diet plus treatments (36 birds selected randomly)
 The treatments were included Control (without selenium supplement), Sodium
Selenite SSe (basal diet with 0.3mg sodium seleinate of per Kg of diet) and nano
selenium nano-Se (basal diet with nano selenium 0.3mg of per kg)

6. Experimental design
Groups Control SSe Nano-Se
Thermoneutral
(22±1℃)
6 birds 6 birds 6 birds
High ambient temperature
(35±1℃ for 9 h)
6 birds 6 birds 6 birds
Relative humidity - 50% to 70%
Size of Se nanoparticles - 100 to 500 nm
Light – 24 hours

7. Parameters
• Growth performance
• Antioxidant status
• Immunity
• Muscle Se concentration

8. Statistical Analysis
• Differences among treatments were analyzed as a 3×2
(diet×temperature) factorial arrangement of treatments by
two-way ANOVA
• When a significant interaction was observed, the means for
each treatment were compared using Tukey’s multiple
comparison test
• P≤0.05 was setas the limitof significance

9. Results- Growth performance

10. Results- Antioxidant status

13. Results- Immunity

14. Result- muscle Se content

15. Conclusion
• The results of the this study suggest that dietary
supplementation with nano-Se (0.3 mg/kg diet) may improve
growth performance, antioxidative status, and immunity in
broilers reared under high ambient temperature conditions