The document summarizes key information about blood cells and cell structure as seen under light and electron microscopes. It discusses how blood cells are viewed using magnification and staining. It also describes the structures and functions of the plasma membrane, including the fluid mosaic model, and compares animal and plant cell structures beyond what can be seen with light microscopy alone.

1. Blood

4. How do we see what’s in our blood? Magnification Staining

8. e

10. Spot the difference! More info to follow in lesson 4

11. Using a haemocytometer

23. Microscopy The light microscope v the electron microscope

24. Optical microscope Light microscope allows you to see the position of the cell membrane, cytoplasm, and nucleus in eukaryotic cells. Small organelles can not be seen clearly.

26. Light Microscopy: This is the oldest, simplest and most widely-used form of microscopy. Specimens are illuminated with light, which is focussed using glass lenses and viewed using the eye or photographic film. Specimens can be living or dead, but often need to be stained with a coloured dye to make them visible. Many different stains are available that stain specific parts of the cell such as DNA, lipids, cytoskeleton, etc. All light microscopes today are compound microscopes , which means they use several lenses to obtain high magnification. Light microscopy has a resolution of about 200 nm, which is good enough to see cells, but not the details of cell organelles.

33. Transmission EM

37. Scanning EM

38. Scanning EM

43. LEUCOCYTES

46. Granulocytes

48. Lymphocytes

50. Monocytes

52. What are these? Giant sized Normal sized PLATELETS- initiate blood clotting

53. Cell structure – beyond light microscopy

56. We are examples of Protista!

57. We are .....

58. We are ...

59. Plasma membrane Cytoplasm Nucleus EUKARYOTIC

60. Animal cell

61. Cell wall Cell membrane Vacuole Nucleus Chloroplast Starch grain EUKARYOTIC

62. Plant cell

69. The Plasma Membrane

74. Cell membrane at approx 240 000 x magnification (electron microscope)

75. Cell membrane at a lower magnification Lighter area = extracellular space

76. How the membrane becomes more fluid at higher temperatures

77. Cholesterol