1. The cell was first observed and named by Robert Hooke in the 1600s. Theodor Schwann and others later established the Cell Theory which states that cells are the basic unit of life, cells only come from existing cells, and all cells contain the basic machinery to carry out life functions.
2. Cells are typically small, around 10 micrometers, because their surface area to volume ratio allows for efficient exchange of materials. Larger cells would not be able to support their inner volumes.
3. The light microscope allows observation of cells at low magnifications while the electron microscope provides higher resolution images at the molecular level. Various structures within cells, like organelles, carry out specific functions to keep the cell alive.
The word cell is derived from the Latin word “cellula” which means “a little room”
It was the British botanist Robert Hooke who, in 1664, while examining a slice of bottle cork under a microscope, found its structure resembling the box-like living quarters of the monks in a monastery, and coined the word “cells”
The word cell is derived from the Latin word “cellula” which means “a little room”
It was the British botanist Robert Hooke who, in 1664, while examining a slice of bottle cork under a microscope, found its structure resembling the box-like living quarters of the monks in a monastery, and coined the word “cells”
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
4. THE CELL THEORY
1. Cells are the smallest units of life.
2. Cells come only from existing cells
3. Cell is the unit of function of all living cells
Theodor Schwann Matthais Schleidon Rudolf Virchow
5. Are there exceptions to the cell theory??
No protoplasm No definite nucleus
One celled
Many nuclei
No nucleus
7. Why are cells so small?
The functions of cell’s volume-
– The rate of heat and waste production and
– Rate of resource consumption.
As the width increases the surface area also increases, but it is slower
than the increase in volume.
As a cell grows larger at some point its surface area becomes too
small to allow these materials to enter the cell quickly enough to
meet the cell's need.
Rate of diffusion α Surface Area x Concentration Difference
Distance
8. CELL SIZE
1. A few types of cells are large enough to be seen by the unaided eye.
The human egg (ovum) is the largest cell in the body, and can (just)
be seen without the aid of a microscope.
2. Most cells are small for two main reasons:
a). The cell’s nucleus can only control a certain volume of active
cytoplasm.
b). Cells are limited in size by their surface area to volume ratio. A
group of small cells has a relatively larger surface area than a
single large cell of the same volume. This is important because
the nutrients, oxygen, and other materials a cell requires must
enter through it surface.
9. • Molecules of Biological significance are around 1 nm in
size where as the cell membrane is about ten times
thicker at 10nm.
• Where as a virus is ten times larger again at around
100nm.
• where as a bacteria is ten times larger again at around 1
um.
• where as a eukaryotic animal cell is is ten time larger
again at around 10 um.
• where as a eukaryotic plant cell is ten times larger again
at around 100 um.
Relative sizes:
1. molecules (1nm).
2. cell membrane thickness
(10nm).
3. virus (100nm).
4. bacteria (1um).
5. organelles (less 10um).
6. cells (<100 um).
7. generally plant cells are
larger than animal cells.
11. Simple and compound microscope.
Simple
microscopes:
Consist of a
single lens.
Compound
microscopes:
Consist of more
than one lens
12. MICROSCOPE
• Two properties:
1. Magnification: the ability of the microscope
to increase the size of the image of an object
formed on the retina of the eye.
2. It can be represented as:
MP =Size of retinal image formed with the microscope ( size of image)
Size of retinal image formed with unaided normal eye (size of specimen)
________________________________________
13. TGES BIOLOGY IBDP
Resolving power
• The ability of a microscope or any magnifying instrument to
separate or distinguish between two closely placed points.
• This depends on the wave length and the light gathering
capacity of the objective lens- numerical aperture.
14. Light microscope:
The source of
illumination is light.
Electron microscope:
The source of
illumination is electron
beam.
27. CYTOPLASM
• Homogeneous translucent, jelly like part of the
protoplasm after the cell organelles are removed.
Functions-
• Intracellular distribution of substances
• Exchange of materials between organelles
28.
29. ENDOPLASMIC RETICULUM
• All cells except RBC, eggs,
embryonic cells & resting
cells
• Made up of –
– Cisternae,
– tubules and
– vesicles
• Types–
• SER –makes lipids modifies
proteins.(adipose, liver cell)
• RER- studded with
ribosomes and makes
proteins
30. MITOCHONDRIA
• Absent in prokaryotes,
lost in RBC.
Outer chamber or perichondrial
space
Algae with one
mitochondria
Microsterias
Highest number of
mitochondria in insect flight
muscles.
31. MITOCHONDRIA
Ultra structure
• Outer mitochondrial
membrane.
• Inner membrane–
• Inner membrane has
folds– cristae– studded
knob-like particles
called elementary
particles or oxysomes.
Outer chamber or perichondrial space
34. Chloroplast
• Structure:
– Double membrane
envelope-peri-
plastid space
– The stroma or
matrix
– Grana –contains
sac like thylakoids
35. CHLOROPLASTS: AUTONOMY AND FUNCTION
• Manufacture some
proteins
• New arise from division
of older ones
Functions:
• Photosynthesis
• Oxygen supply
• Storage of starch
temporarily
• Fixation of CO2
• Greenery
• Chromoplasts
36. LEUCOPLASTS
Colourless plastids
No grana and
photosynthetic
pigments.
They are of three
types
■ Amyloplasts –
starch containing
■ Proteoplasts-
stores proteins
■ Elaioplasts-fat
storing
37. CHROMOPLAST
Chromoplast
• Coloured plastids
• Contain –carotenoids
• Functions
■ Impart colours to flowers
■ Bright colour to fruits
■ When green carry out photosynthesis
■ Sites of synthesis of membrane lipids
40. LYSOSOMES
• LYSOSOMES
• Rounded or spherical with single
membrane containing acid hydrolases.
■ Structure: Two subunits- Large
subunit and small subunit
■ Present freely in the cytoplasm.
■ FUNCTION- SITE OF PROTEIN
SYNTHESIS
RIBOSOMES
41. Centrioles
• Non membranous cell organelle.
• Occur in pairs- diplosome
• Occur at right angles to each other in the
cytoplasm –centrosphere.
• Centriole and centrosphere- centrosome
• Function- initiates cell division
42. VACUOLES
• Sap filled vesicles in
the cytoplasm
• Bounded by
tonoplast, fluid- cell
sap
43. NUCLEUS
Structure:
• 1. Nuclear envelope or karyotheca
• 2. Nucleoplasm -karyolymph
• 3. Chromatin net
• 4. Nucleolus
Occurrence:
• Absent in bacteria, BGA
(prokaryotes)
• Present in all eukaryotes