Northwestern blotting is a technique used to detect interactions between RNA-binding proteins and RNA molecules. It involves separating proteins by electrophoresis, transferring them to a membrane, and incubating the membrane with a labeled RNA probe. This allows RNA-binding proteins to be identified based on their ability to interact with the probe. While the technique can detect RNA-protein binding, it does not identify the specific RNA sequences bound. Other methods may be used along with Northwestern blotting to determine a protein's specific RNA targets.