Optics beyond the Abbe Limit
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Present State of Super Resolution Far Field Light Microscopy realized in the Christoph Cremer Lab (December 2011) Optical Resolution OR (resolvable distance): OR ~ 5 nm (~ 1/100 λexc, from localization precision) Structural Resolution (imaging capability): ● Mean (2D) distance between individual molecules actually detected: ~ 6 nm ( ~ 1/80 λexc) ● Density of individually detected Molecules : ~ 2,8 • 104/μm2 – Multicolor- Localization Microscopy: ● 2 – 3 different molecules types ● 3D single Molecule Resolution inside cells (best values): 3D Observation Volume about 1*104 times smaller (i.e. 3D resolution 104 times better) than in conventional Light Microscopy (“Abbe-limit”) Super Resolution Microscopy with viruses (single molecule imaging)
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Super Resolution Microscopy Christoph Cremer New Year 2014
Some exciting results of the Cremer lab in 2013: super resolution microscopy of mouse pachytene chromosomes, structured illumination microscopy in human retina diagnostics & combination of structured illumination and single molecule localization microscopy in one setup
1.22.2010
There are several key principles of microscopy. Thicker samples require sectioning to be accurately imaged. Various microscopy techniques like light, fluorescent, and electron microscopy can be used to image fixed or live samples. Confocal live imaging uses layered excitation of fluorescent molecules to visualize distinct optical layers within tissue. The resolution, contrast, magnification, and type of microscopy technique used depend on the size and features of structures needing examination.
Modern Techniques of Materials Characterisation
The document discusses various materials characterization techniques. It provides details on several microscopy techniques like metallography, polarized light microscopy and interference microscopy. It also describes various principles of X-ray diffraction techniques like Bragg's law and applications for structure determination. Additionally, the document outlines electron microscopy techniques like TEM, SEM and their components. It covers principles of various analytical techniques like X-ray spectroscopy, thermal analysis methods like DTA, DSC and TGA.
xrd rrn 03.pptx
This document discusses X-ray diffraction, which uses X-rays to determine the atomic structure of crystals. It explains the basic principles of XRD, including Bragg's Law which relates the diffraction angle to atomic spacing. The key instrumentation of X-ray diffractometers is described, including the X-ray tube, sample holder, and detector. Two common methods are powder XRD which uses small samples, and rotating crystal which orients single crystals. Applications include determining protein structures, distinguishing crystal structures, and studying crystal properties and deformation.
Content-based Image Retrieval
The project aims at development of efficient segmentation method for the CBIR system. Mean-shift segmentation generates a list of potential objects which are meaningful and then these objects are clustered according to a predefined similarity measure. The method was tested on benchmark data and F-Score of .30 was achieved.
Week 2. Diffusion magnetic resonance imaging, tractography, mapping the brain...
The document summarizes key points about multimodal neuroimaging techniques, with a focus on diffusion magnetic resonance imaging (MRI) and fiber tracking. It discusses how diffusion MRI can be used to measure and visualize water diffusion in the brain, which provides information about tissue microstructure and white matter pathways. Specifically, it describes diffusion tensor imaging (DTI) and how it is used to quantify diffusion anisotropy and direction. The clinical and research applications of DTI and fiber tractography are also summarized, such as characterizing white matter integrity and disorders, assessing brain tumors, and mapping brain connectivity and development.
Confocal microscopy by prabhat
Confocal microscopy was invented by Marvin Minsky in 1957 and aims to improve resolution over traditional microscopy. It uses point illumination and a pinhole to exclude out-of-focus light and produce thin optical sections and high-contrast images. The key components are a laser light source, dichromatic mirror, pinholes, and photodetector. Confocal microscopy finds applications in cell biology and materials science by allowing optical sectioning and 3D reconstruction. It provides advantages like non-invasiveness, live cell imaging, and depth analysis, but has disadvantages such as photobleaching and loss of intensity.
nano imaging its potential and future.pptx
This document provides an introduction to bionanoimaging. It discusses various nanoimaging techniques such as scanning probe microscopy, transmission electron microscopy, and scanning electron microscopy. It also discusses different materials used in nanoimaging like quantum dots, which can be tuned to emit specific wavelengths of light depending on their size. New approaches in nanoimaging include using contrast agents for ultrasound imaging to overcome limitations of traditional ultrasound and achieve improved resolution for deeper scans. Applications of nanoimaging span various fields like materials science, biology, medicine, electronics, and nanotechnology.
Nano-bio Dr Nedra Karunaratne
This document discusses various applications of nanotechnology in biology and medicine, including fluorescent biological labels, MRI contrast enhancement, biodetection of pathogens, detection of proteins, separation and purification of biomolecules and cells, probing of DNA structure, tumour destruction via heating, tissue engineering, biosensors, biomineralization, and drug and gene delivery. Quantum dots are discussed as an example of fluorescent biological labels. Carbon nanotubes have potential uses as nanoelectrodes, in lab-on-a-chip applications, DNA sequencing, artificial muscles, and vision chips. Commercial exploration of nanotechnology includes uses in toothpaste, cell separation, luminescent biomarkers, MRI shielding, implants, and drug/gene delivery.
Comparison of 7 Epithermal Neutron Beams
This document compares the dosimetric characteristics of seven epithermal neutron beams used for boron neutron capture therapy (BNCT) clinical trials in Sweden, Finland, Czech Republic, Netherlands, and United States. Measurements were taken of neutron fluence and absorbed dose in air and in a water phantom using standardized methods. Results showed the fast neutron and photon contamination levels varied between facilities, as did the epithermal neutron flux intensities available. However, penetration depth was sufficient (>8 cm) for treating brain lesions at the midline for all beams. The data provide the first consistent measurement of beam performance across centers and will help normalize calculated patient dosimetry between facilities.
Research statement
The document provides details about the research background and interests of researcher Fang Can. It outlines his educational background in electrical engineering and computer science. It also describes his technical skills in mathematics, optimization, algorithm development, and optics. The document discusses Fang's PhD research projects which took graph-theoretic and geometric approaches to problems in communication networks and wireless sensor networks. It provides examples of his current work developing multi-spectral optical probes and a spectrum-scanning microscope to analyze tissue and cell samples.
Introduction to material science What kind of analyses methods will .pdf
Introduction to material science What kind of analyses methods will you take to examine the
lattice constants surface morphology with 1-10 mu m dislocation preferred orientation?
Solution
a) For lattice constant X-ray diffraction (XRD) is the best technique to analyse.
The atomic planes of a crystal cause an incident beam of X-rays to interfere with one another as
they leave the crystal. This phenomenon is known as X-ray diffraction. X-ray diffraction has
been in use in two main areas, for the fingerprint characterization of crystalline materials and
determination of their structure. Each crystalline solid has its unique characteristic X-ray powder
pattern which may be used as a “fingerprint” for its identification.
b) For surface morphology Scanning electron microscopy (SEM) is the best technique to analyse.
Scanning electron microscope is an instrument where entire primary electron beams emitted
from an electron source are scanned on sample and a scanning image is obtained from a
detection signal produced by secondary particles generated from the sample. Scanning electron
microscope actually capable of obtaining a scanning image with high spatial resolution within a
low acceleration voltage region
c) For dislocation Weak-Beam Dark-Field is the best methdo to analyse.
d)For orientation Geiger counter xray spectrometer is the best device to use..
Priya application of quantum dots in biological sciences
This document discusses the application of quantum dots in biological sciences. It begins by explaining that quantum dots are nanocrystals made of semiconductor materials that glow when exposed to light and have size-dependent fluorescent properties. It then outlines several applications of quantum dots, including their use in cell imaging, cancer treatments, biosensors, and diagnostics like immunolabeling and nucleic acid detection. The document concludes that quantum dots are a leading research tool that can be applied to fields like human health, disease, robotics, and the environment due to their unique optical and physical properties.
Lensfree Microscopy and Tomography
This document discusses lensfree microscopy and tomography techniques developed by Serhan Isikman for biomedical applications. [1] Lensfree microscopy uses holograms recorded by a sensor array to digitally reconstruct microscope images over a wide field of view in a compact, low-cost system. [2] It has been used to rapidly count red blood cells on a chip with high accuracy. [3] Lensfree optical tomography similarly uses holograms from multiple angles to computationally generate 3D images without lenses, achieving micrometer-scale resolution.
Phil. Trans. R. Soc. B-2013-Michalet-
This document discusses developments in photon-counting detectors for single-molecule fluorescence microscopy. It describes two common optical configurations used: point-like excitation and detection of freely diffusing molecules, and wide field illumination and detection of surface-immobilized molecules. Each approach currently uses different optimal detectors, but there is room for improvement. Recent developments aim to increase the throughput of single-molecule fluorescence spectroscopy using parallel arrays of single-photon avalanche diodes, and develop large-area photon-counting cameras for fluorescence lifetime imaging at the single-molecule level with sub-nanosecond resolution.
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Super Resolution Microscopy Christoph Cremer New Year 2014
Super Resolution Microscopy Christoph Cremer New Year 2014
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Week 2. Diffusion magnetic resonance imaging, tractography, mapping the brain...
Introduction to material science What kind of analyses methods will .pdf
Introduction to material science What kind of analyses methods will .pdf
Priya application of quantum dots in biological sciences
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More from Dr. Andrea Nestl
S3 l stromumwandler tlb
S3L-Inverter / Schaltverlustfreier 3-Stufen-Pulswechselrichter mit Entlastungsschaltung
Der neue 3-Stufen-Pulswechselrichter / „S3L-Inverter (Soft Switching Three Level Inverter) ist von bestechender Einfachheit und somit geringen Kosten. Er arbeitet prinzipbedingt verlustfrei, weist somit höchste Wirkungsgrade auf, wird auf einfache Weise gesteuert, hat EMV- freundliche inhärente di/dt - und du/dt – Begrenzungen und kann mit preisgünstigen Standard - Halbleitern aufgebaut werden.
Anwendungsgebiete sind: Elektrische Antriebe, Solarwechselrichter, Windkraftwechselrichter, Unterbrechungsfreie Stromversorgungen.
S3 l inverter tlb
The new Soft Switching Three Level Inverter (S3L – Inverter) is of striking simplicity and is therefore inexpensive. Because of the underlying principle, it works without losses and thus with maximum efficiency. The inverter is easily controlled, has EMC friendly inherent di/dt- and du/dt limitation, and can be built using low-cost standard semiconductors.
Field of use: electric drives, solar power inverter, wind power inverter, uninterruptible power supplies (UPS).
Miniaturized light sheet based fluorescence microscopy Schneckenburger
A miniaturized setup for light sheet based fluorescence microscopy is adapted to a conventional microscope and used to measure the uptake and interaction of cytostatic drugs, metabolites and fluorescent dyes in 3D cell cultures. FOM 2013 [P1-D/07] A light sheet module for 3D fluorescence microscopy of tumour cell spheroids: H. Schneckenburger & S. Schickinger & M. Wagner & P. Weber & R. Wittig & T. Bruns (Hochschule Aalen, Germany)
TLB GmbH Newsletter Feb 2013
Themen; Berthold-Leibinger-Innovationspreis, Stahl Innovationspreis, optimierte Aquakultur, Extoll-Ausgründung, Businessdevelopment, Patentseminare, Markerfreies Nanoskop auf Hannovermesse
TLB Patentseminar an der Hochschule Karlsruhe
Erfindercafé an der Hochschule Karlsruhe – Technik und Wirtschaft
Die Vorträge und die Podiumsdiskussion zur Patentierung und Verwertung von Erfindungen sind speziell auf die Anforderungen von Erfindern der Hochschule Karlsruhe abgestimmt.
Die Veranstaltung unterstützt Wissenschaftler und Wissenschaftlerinnen in ihrem innovationsorientierten Denken und verdeutlicht die Bedeutung von Schutzrechten zur Absicherung von Forschungsergebnissen.
Innovative UV Lampe hilft bei Hygiene energiesparen
Vorteile
• Energierückgewinnung / Energieeinsparung
• Hoher Wirkungsgrad
• Flexibilität / Anpassungsmöglichkeit
• Hochfrequenzbetrieb
Universell einsetzbar
• Lebensmittelhygiene
• Medizintechnik
• Oberflächenbehandlung
• Halbleiterproduktion
Tlb Newsletter Juni 2012
Inhalt: Solarenergie, Unsichtbare Materialien, Jugendpillen, Oloid, interaktive Patentveranstaltung, F & E Kooperationen, Vorstellung neuer CEO Marcus Lehnen, Verabschiedung scheidender CEO Arno Basedow
Redaktion Dr. Regina Kratt
Variable Anströmung für Abgasturbolader MEDUSA
DIe Innovation holt mehr Drehmoment aus dem Motor + bewirkt eine Benzinerparnis
Unterdrückung von Reibschwingungen in KFZ-Kupplungen
Auch bei modernen Kupplungen können Reibschwingungen auftreten, die sich beispiels-weise durch „Rupfen“ und Vibrationen zeigen bzw. störende Geräusche verursachen. Durch die Innovation werden Schwingungen soweit unterdrückt, dass sie nicht mehr wahrgenommen werden.
Ihre Vorteile auf einen Blick:
• einfacher Aufbau
• wenig Zusatzkosten
• geringer Platzbedarf
• Begrenzung der Schwingungsamplitude
• Reibschwingungen werden wirksam unterdrückt
Heizpatrone heizkapsel gezieltes heizen von spritzgusswerkzeugen
Das Heizen von Spritzgusswerkzeugen an kritischen Stellen ist aufwändig, da Anpassungen am Werkzeug vorgenommen werden müssen. Eine induktiv erwärmte Heizpatrone heizt gezielt an diesen Stellen. Außerdem muss nicht das gesamte Werkzeug geheizt werden. Dieses spart Energie und erhöht den Durchsatz.
Prozessor mit integrierter, echtzeitfähiger speicherbereinigung
Damit lässt sich das Problem des Datenmülls beheben, der sich im Speicher angesammelt hat und den Computer langsam macht
Vorteile
geeignet für Systeme mit harten Echtzeitanforderungen
(z. B. in Embedded Systems)
geringer Overhead für Speicherbereinigung
keine Unterbrechungen durch die Speicherbereinigung
extrem robust durch Speicherschutz auf
Objektebene
funktionsfähiger Prototyp
(Prozessor und Java-Compiler)
Processor with integrated real time garbage collection
Collect garbage that has accumulated in the memory and makes your computer slow
Your Benefits at a Glance
Suitable for systems with hard real-time requirements
(in particular embedded systems)
Low garbage collection overhead
No interruptions caused by garbage collection
Extremely robust due to object-based memory
protection
Fully operational prototype (including a Java
compiler)
Presentation processor with integrated real time garbage collection
Collect garbage that has accumulated in the memory and makes your computer slow
Your Benefits at a Glance
Suitable for systems with hard real-time requirements
(in particular embedded systems)
Low garbage collection overhead
No interruptions caused by garbage collection
Extremely robust due to object-based memory
protection
Fully operational prototype (including a Java
compiler)
Wer nicht erfindet, verschwindet. wer nicht patentiert, verliert (erich haeus...
Patentseminar für Hochschulerfinder an der Hochschule Reutlingen
Am 19. April 2012 findet an der Hochschule Reutlingen eine Veranstaltung mit Vorträgen und einer Podiumsdiskussion zu den Themen Patentieren, Publizieren und Industriekooperationen statt. Die Veranstaltung wird von der Hochschule Reutlingen in Zusammenarbeit mit der TLB durchgeführt.
Das Programm ist speziell auf die Anforderung von Erfindern an der Hochschule Reutlingen abgestimmt.
Cell free GPCR ligand screening assay tlb
GPCR ligand screening assay
Monitors the very early activation stage of Frizzled-Receptors of the GPCR group
Our assay can be implemented in a cell-free chemical compound high-throughput target screening. GPCR is the largest known category of molecular targets with verified therapeutic value. Up to 40% of approved drugs are targeted to GPCRs. 25% of the top 200 best-selling drug targets are GPCRs.
Neuartige Doppelmutter für Kugelrollspindel verlängert die Lebensdauer
Je kleiner man also die Vorspannung – mit einer
gleichzeitigen Sicherung der geforderten Steifigkeit –
einstellen kann, desto kleiner wird die mechanische
Belastung und desto größer die zu erwartende
Lebensdauer. Es wird eine Restvorspannkraft in der entlasteten
Mutterhälfte erzeugt, wodurch sichergestellt wird, dass es
nicht zu übermäßigen Gleitbewegungen kommen
kann
Mikroskope und Mikroben (Super Resolution Mikroskopie von Bakteren & Viren)
Christoph Cremer, Universität Heidelberg:
Mikroskope und Mikroben, ein Rundgang durch die Medizingeschichte
Überall lauern Mikroorganismen, manchmal auch „Mikroben“ genannt, die Gesundheit und Leben von Menschen, Tieren und Pflanzen bedrohen. Es handelt sich dabei um mikroskopisch kleine Lebewesen, die als einzelne Individuen mit bloßem Auge nicht zu erkennen sind. Beispiele sind Bakterien, die für die Menschheitsgeißeln Pest, Cholera oder Syphilis verantwortlich sind. Zu den „Mikroben“ werden oft auch als ebenfalls submikroskopisch kleine Krankheitserreger die Viren gezählt, z.B. als Verursacher von Grippe, Gelbfieber, Pocken, AIDS, oder bestimmten Formen von Krebserkrankungen.
Low-cost production of therapeutics
This novel process for the purification of proteins, which is both highly efficient and low cost
•Shortening of the downstream protein purification processes
•Substantial cost and time savings
•Capable of being implemented on an industrial scale
•Fast kinetics
KMU-Erfolg durch Innovationsmanagement
Schutzrechte sind aktive Werte für Ihr Unternehmen
Kluge Köpfe, die ihre Ideen umsetzen, sind Ihr größtes Unternehmenskapital. TLB übernimmt die Erstellung gutachterlicher
technologischer Bewertungen von zum Patent angemeldeten Erfindungen oder bereits erteilten Patenten. Dabei arbeitet TLB mit Wirtschaftsprüfungsgesellschaften, Beratungsgesellschaften und spezialisierten Patentanwälten zusammen.
Yoga + Raw Flyer
Integration von Yoga und Raw Food in den eigenen Lifestyle. Yoga hat in der Welt der Erfolgreichen und Kreativen längst seinen festen Platz. Der noch junge
Trend, sich rohköstlich zu ernähren, bahnt sich mit prominenten Vorreiter wie Madonna, Anthony
Robbins, Demi Moore und Nena gerade schwungvoll seinen Weg. Seminare & Kurse
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Miniaturized light sheet based fluorescence microscopy Schneckenburger
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Innovative UV Lampe hilft bei Hygiene energiesparen
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Unterdrückung von Reibschwingungen in KFZ-Kupplungen
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Processor with integrated real time garbage collection
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Presentation processor with integrated real time garbage collection
Wer nicht erfindet, verschwindet. wer nicht patentiert, verliert (erich haeus...
Wer nicht erfindet, verschwindet. wer nicht patentiert, verliert (erich haeus...
Neuartige Doppelmutter für Kugelrollspindel verlängert die Lebensdauer
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Alt. GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using ...
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
DevOps and Testing slides at DASA Connect
My and Rik Marselis slides at 30.5.2024 DASA Connect conference. We discuss about what is testing, then what is agile testing and finally what is Testing in DevOps. Finally we had lovely workshop with the participants trying to find out different ways to think about quality and testing in different parts of the DevOps infinity loop.
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Optics beyond the Abbe Limit
- 1. January 1, 2012 Letizia Mancino Heidelberg Jackson Lab Mainz Optics beyond the Abbe Limit: “Molecular Constellations“ C. Cremer Institute of Molecular Biology (IMB), Mainz/DE Institut for Pharmacy & Molecular Biotechnology; Kirchhoff-Institute of Physics, University Heidelberg; Institute for Molecular Biophysics, The Jackson Laboratory,ME/USA For more information see www.imb-mainz.de
- 2. Conventional Resolution Localization Microscopy From: C. Cremer et al., Biotechnology J. 6 (2011) 200 nm___ From: All scale bars 500 nm Y. Markaki et al., CSH Symposia 75 (2011) 100 nm Above: Viruses; Below: 2 Protein types within a human cell nucleus. The crosses/circles denote positions of single molecules (exciting wavelength 488 nm)
- 3. Principle of Spectrally Assigned Localization Microscopy (SPDM): Optical Isolation of Diffraction Images of individual Molecules: Determination of Gravity Centers 3 point-like objects in x,y plane with next neigbour distances 50 nm a) Labelling with same spectral signature b) Labeling with different unique spectral signatures B,G,R Computation (scalar Theory): NA = 1.4, λexc = 488 nm Optical Isolation by the “Supernova“ Mode: Induction of stochastically distributed short flashes of light emission by Cremer et al. 1996, 1999, 2010, 2011 individual molecules
- 4. Present State of Superresolution Far Field Light Microscopy realized @ C. Cremer Lab (December 2011) - Optical Resolution OR (resolvable distance): OR ~ 5 nm (~ 1/100 λexc, from localization precision) - Structural Resolution (imaging capability): ● Mean (2D) distance between individual molecules actually detected: ~ 6 nm ( ~ 1/80 λexc) ● Density of individually detected Molecules : ~ 2,8 · 104/µm2 - Multicolor- Localization Microscopy: ● 2 – 3 different molecules types ● 3D single Molecule Resolution inside cells (best values): 3D Observation Volume about 1*104 times smaller (i.e. 3D resolution 104 times better) than in conventional Light Microscopy (“Abbe-limit”)
- 5. Some recent references: C. Cremer, Optics far Beyond the Diffraction Limit: From Focused Nanoscopy to Spectrally Assigned Localization Microscopy. Springer Handbook of Lasers and Optics, 2nd edition (F. Träger, Edit.), in press. Y. Markaki et al. (2011) Functional nuclear organization of transcription and DNA replication: a topographical marriage between chromatin domains and the interchromatin compartment. Cold Spring Harbor Symposia on Quantitative Biology 75: 1–18. doi:10.1101/sqb.2010.75.042. R. Kaufmann et al. (2011) Analysis of Her2/neu membrane protein clusters in breast cancer cells using localisation microscopy. J. of Microscopy 242: 46–54. G. Best et al. (2011) Structured illumination microscopy of autofluorescent aggregations in human tissue. Micron 42: 330–335. C. Cremer et al. (2011) Superresolution imaging of biological nanostructures by spectral precision distance microscopy, Biotechnology 6: 1037–1051. C. Cremer (2011) Lichtmikroskopie unterhalb des Abbe-Limits, Physik in Unserer Zeit 42: 21 – 29.