UHPLC/UPLC: Ultra High Performance Liquid ChromatographyDarewin Mendonsa
Chromatography Techniques mainly include two basic sub-divisions: Separation Science and Analytical Science.
In 2004, separation science was revolutionized with the introduction of ‘Ultra High-Performance Liquid Chromatography which provides improved resolution, increased separation efficiency, shorter analysis time and lower operating costs.
It uses HPLC columns with a mean particle size less than 2μm and pressures up to 15,000 psi which drastically increases the number of theoretical plates of the column and results in enhanced column efficiency.
Fast, selective, and sensitive methods can be developed for the analysis of impurities
Offering many business benefits using UPLC and UPC2
Increase in sample throughput
Reduction in toxic solvent usage
Using mass spectral detection over UV detection provides
Improvement in sensitivity and selectivity
Reduced matrix effects
PDA and mass detection provide complementary information for peak assignment and structural confirmation of impurities
Many people pursue ideas of “efficiency” as an ideal for daily life; the same can be true in the HPLC laboratory. In this work, we demonstrate the efficiency, throughput, and reliability of a dual injection system for finished pharmaceutical products and in-process active pharmaceutical ingredients
This presentation covers an introduction to UPLC, its general chemistry, and laws behind it. It also discusses the instrumentation of UPLC, advantages, disadvantages, and application of UPLC.
HPLC[ HIGH PERPROMANCE LIQUID CHROMATOGRAPHY OR HIGH PRESSURE LIQUID CHROMAT...Dr. Ravi Sankar
GASSCHROMATOGRAPHY[GC], ADVANCED STUDY OF THE FOLLOWING AND THEIR APPLICATIONS, INTRODUCTION, THEORY, COLUMN OPERATION,INSTRUMENTATION AND DETECTION,APPLICATIONS AND ADVANTAGES OF GC,PRINCIPLE OF SEPARATION IN GC, HOW GC MECHINE WORKS? COLUMN, DETECTORS.
BY P.RAVISANKAR, VIGNAN PHARMACY COLLEGE, VADLAMUDI, GUNTUR, A.P, INDIA.
Industrial Applications Of Chromatography Techniquerita martin
Chromatography Technologies plays vital role in various industrial application sectors, this techniques as been used across various chemical and pharmaceutical industries. This technique is used to purify sugar from molasses, separation of enantiomers and purification of pharmaceutical proteins, pharmaceutical industry for the purification of enantiomers from racemic mixtures. Its applications were also used in food chemistry, biochemistry, petro chemistry, pharmaceutical chemistry. Also plays important roles in Purification of proteins, pharmaceuticals, fine chemicals
UHPLC/UPLC: Ultra High Performance Liquid ChromatographyDarewin Mendonsa
Chromatography Techniques mainly include two basic sub-divisions: Separation Science and Analytical Science.
In 2004, separation science was revolutionized with the introduction of ‘Ultra High-Performance Liquid Chromatography which provides improved resolution, increased separation efficiency, shorter analysis time and lower operating costs.
It uses HPLC columns with a mean particle size less than 2μm and pressures up to 15,000 psi which drastically increases the number of theoretical plates of the column and results in enhanced column efficiency.
Fast, selective, and sensitive methods can be developed for the analysis of impurities
Offering many business benefits using UPLC and UPC2
Increase in sample throughput
Reduction in toxic solvent usage
Using mass spectral detection over UV detection provides
Improvement in sensitivity and selectivity
Reduced matrix effects
PDA and mass detection provide complementary information for peak assignment and structural confirmation of impurities
Many people pursue ideas of “efficiency” as an ideal for daily life; the same can be true in the HPLC laboratory. In this work, we demonstrate the efficiency, throughput, and reliability of a dual injection system for finished pharmaceutical products and in-process active pharmaceutical ingredients
This presentation covers an introduction to UPLC, its general chemistry, and laws behind it. It also discusses the instrumentation of UPLC, advantages, disadvantages, and application of UPLC.
HPLC[ HIGH PERPROMANCE LIQUID CHROMATOGRAPHY OR HIGH PRESSURE LIQUID CHROMAT...Dr. Ravi Sankar
GASSCHROMATOGRAPHY[GC], ADVANCED STUDY OF THE FOLLOWING AND THEIR APPLICATIONS, INTRODUCTION, THEORY, COLUMN OPERATION,INSTRUMENTATION AND DETECTION,APPLICATIONS AND ADVANTAGES OF GC,PRINCIPLE OF SEPARATION IN GC, HOW GC MECHINE WORKS? COLUMN, DETECTORS.
BY P.RAVISANKAR, VIGNAN PHARMACY COLLEGE, VADLAMUDI, GUNTUR, A.P, INDIA.
Industrial Applications Of Chromatography Techniquerita martin
Chromatography Technologies plays vital role in various industrial application sectors, this techniques as been used across various chemical and pharmaceutical industries. This technique is used to purify sugar from molasses, separation of enantiomers and purification of pharmaceutical proteins, pharmaceutical industry for the purification of enantiomers from racemic mixtures. Its applications were also used in food chemistry, biochemistry, petro chemistry, pharmaceutical chemistry. Also plays important roles in Purification of proteins, pharmaceuticals, fine chemicals
A presentation outlining the various processes a chemical compound undergoes (thorough & rigorous screening procedures) before it is finally introduced into the drug market
UPLC is an advance analytical technique where it takes advantage of innovation in various technologies such as instrumentation and particle size to achieve dramatic increases in resolution, speed and sensitivity of the liquid chromatography. It operates at higher pressure than that used in HPLC and uses fine particles (less than 2.5µm) & mobile phases at high linear velocities. It can be hyphenated with other techniques such as (MS), (IC), (NMR) and (IR) etc. This is used in all industries and has found application in various fields such as pharmaceutical, food, environmental, forensic, toxicology and pesticide.
Recently, a new high-sensitivity detector was introduced that provides improved UHPLC system performance and includes new features that improve workplace efficiency.
This presentation introduces this technology and provides a description of its use for impurity analysis. For more information, go to www.ssi.shimadzu.com or follow Shimadzu on Twitter @shimadzussi.
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This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
ESC Beyond Borders _From EU to You_ InfoPack general.pdf
Mohini patil
1. ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY
(UPLC)
Presented By, Guided By,
Miss. Mohini Patil Dr. Rajesh J Oswal
Prof.Sandip Kshirsagar
Department of Pharmaceutical Chemistry
6/13/2012 1
2. INTRODUCTION
PRINCIPLE
INSTRUMENTATION
EXAMPLE OF UPLC SEPRATION
COMPARISON BETWEEN HPLC & UPLC
SEPRATION
APPLICATIONS
6/13/2012 2
3. INTRODUCTION:
UPLC provides improved resolution, speed, and
sensitivity
By using conventional particle
sizes, speed, pressures and peak capacity can be
extended to new limits and compromises with
sacrificing retention time.
As the particle size is 1.7µm there is a significant
gain in efficiency along with resolution and without
diminish efficiency. (Figure 1)
6/13/2012 3
4. Figure1. Van Deemter plot, illustrating the evolution of
particle
According to figure 1 ,
column efficiency (N) is inversely proportional to particle
size (dp).
Thus, smaller particles provide higher resolution and lower
retention time.
6/13/2012 4
5. PRINCIPLE:
The UPLC is based on the principal of HPLC.
It involves stationary phase consisting of particles sub 2
μm, as HPLC columns are typically filled with particles of 3
to 5 μm.
Efficiency is the primary separation parameter behind
UPLC since it relies on the same selectivity and retentivity as
HPLC.
In the fundamental resolution equation resolution is
proportional to the square root of N.
6/13/2012 5
6. Where,
N is number of theoretical plates,
α is Selectivity factor and
k is mean retention factor.
But since N is inversely proportional to particle size (dp);
N α 1/dp
As the particle size is lowered by thrice i.e. from 5 mm to1.7
mm, N is increased by three and the resolution by square root
of three i.e 1.7
6/13/2012 6
9. 4. Optional Sample Organizer: Sample organizer stores
micro titer or vial plates & transfer them to & from sample
manager.
5. Optical Detectors: The system can be configured with an
TUV (Tunable Ultraviolet), PDA (Photodiode Array) or ELS
(Evaporative Light Scattering) optical detector or any
combination of the three.
6/13/2012 9
10. Column: 2.1 by 30mm 1.7 mm ACQUI-TY UPLC BEH C18
at 358C.
A 20–40% B linear gradient over 1.0 minute, at a flow rate of
0.86mL/min was used.
6/13/2012 10
11. Mobile phase: A was 0.1% formic acid, B was Acetonitrile.
UV detection: at 254 nm and 40pts/sec.
Peaks are in order: 1: 7-hydroxycoumarin glucuronide, 7-
hydroxycoumarin, 4-hydroxy coumarin, coumarin, 7- methoxy
coumarin, 7-ethoxycoumarin, and 4-ethoxycoumarin.
6/13/2012 11
12. Figure shows an HPLC versus UPLC separation comparison
of a ginger root extract sample where both speed and
resolution are improved, as well as an increase in sensitivity.
6/13/2012 12
13. 1. UPLC with MS: UPLC coupled to quadrupole tandem mass
spectrometry which operates with rapid, generic gradients and
shows increase in analytical throughput and also shows
sensitivity in high throughput pharmacokinetics or bioanalysis
studies, the rapid measurement of potential p450
inhibition, induction, and drug-drug interactions had been
studied by UPLC/MS/MS.
2. UPLC in Pharmaceutical Development: Now a day’s UPLC
is a very attractive tool for the pharmaceutical development
laboratory because of high resolution obtained in extremely
short period of analysis times, as UPLC provides high
throughput, high productivity and high resolution
6/13/2012 13
14. 3. UPLC used in Identification of Metabolite: Biotransformation of
new chemical entities (NCE) is necessary for drug discovery. When a
compound reaches the trial stage, metabolite identification is required
and it is necessary for lab to successfully detect and identify all
circulating metabolites of a contender drug.
4. UPLC used. in Bioanalysis / Bioequivalence Studies: For
Pharmacokinetic, Bioequivalence and toxicity studies, the quantitative
analysis of a drug in biological samples is an important part of drug
development process and this is carried out by UPLC
5. UPLC used in stressed degradation Studies: The most common
analytical technique for monitoring forced degradation experiments is
HPLC with UV and/or MS detection for peak purity, mass balance, and
identification of degradation products but these HPLC-based
methodologies are time-consuming and provide only medium
resolution
6/13/2012 14
15. to ensure that all of the degradation products are accurately detected.
PDA/MS (photodiode array and MS) used along with UPLC, which
allows for faster and higher peak capacity separations of complex
degradation product profiles.
7. UPLC used in Impurity Profiling: UPLC PDA detector involves
two analytical flow cells with maximum flexibility and according to
application requirements, as one for maximum chromatographic
resolution and a second for high sensitivity. UPLC also ensure the
latest peak detection algorithms and custom calculations to optimize
data processing and reporting. It also assertively detects impurities in
compounds even at trace levels. To characterize impurities, it is often
necessary to perform several analytical runs to obtain the necessary
MS and MS/MS data.
6/13/2012 15
