This document discusses new developments in high performance liquid chromatography (HPLC) and the role of ultra performance liquid chromatography (UPLC) and nano liquid chromatography in pharmaceutical analysis. It begins with an introduction to HPLC and lists some new developments including UHPLC, HPLC-MS systems, and HPLC chips. It then focuses on UPLC, explaining that it can acquire results faster with more resolution compared to traditional HPLC. UPLC allows for more samples to be analyzed per system and scientist. The document concludes by listing references for further information.

1. New developments in HPLC – role and
principles of Ultra, Nano liquid
chromatography in pharmaceutical analysis
Presented by
G.Pavani
Y17MPHPA426
I/II M.Pharmacy
Department Of
Pharmaceutical Analysis
Chalapathi Institute of pharmaceutical sciences.
Lam,Guntur.

2. Contents
What is HPLC
New developments in HPLC
Role and principle of ultra performance liquid chromatography
Role and principle of nano liquid chromatography

3. What is HPLC

4. New developments in HPLC

5. Continuation
• HPLC –chip/MS system
• UHPLC (Ultra high performance liquid
chromatography)
• HPLC- MS

6. Ultra performance liquid
chromatography

10. Why to migrate from HPLC to UPLC?
Acquire results in less time and with more
resolution.
More information-faster
More robust method-more confidence
Better situational response
More samples analyzed per system, per
scientist

20. References
1. K. Hosoya et al., Anal. Sci. 24, 149 (2008) B.A.
Olsen, B.C. Castle, D.P. Myers, TrAC Trends Anal.
Chem. 25, 796 (2006)
2. P. Sadílek, D. Šatínský, P. Solich, TrAC Trends
Anal. Chem. 26, 375 (2007) H.A
3. . Claessens, M.A. van Straten, J. Chromatogr. A
1060, 23 (2004)
4. L.M. Hewitt, C.H. Marvin, Mutat. Res., Rev.
Mutat. Res. 589, 208 (2005)
5. J. Eijkel, Lab Chip 7, 815 (2007)
http://pubs.rsc.org/