Bacteria have several advantages for genetic experiments, including being haploid, rapid reproduction, and ability to grow in large numbers with genetic identicaly. There are three main types of genetic transfer in bacteria: transformation, where bacteria take up external DNA; conjugation, where DNA transfers between bacteria via sex pili; and transduction, where bacteriophage introduce DNA into bacteria. Frederick Griffith's experiments in 1928 first demonstrated bacterial transformation, showing that a heat-killed pathogenic strain could transform a non-pathogenic strain through DNA. Further work by Avery, MacLeod, and McCarty in 1944 proved that DNA was the genetic material responsible for transformation.
Bacterial genetics /certified fixed orthodontic courses by Indian dental acad...Indian dental academy
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al-salam alykom ..
this lecture starts with basic definitions in genetic , also talk about DNA & RNA ( structures , types , similarities and differences ) .
it talks about bacterial DNA ( chromosome structure / plamids structure and functions / transponon types )
later , discusses about central dogma / gene expression starting from genetic code/codons , then DNA replication , trancription and finally translation
prepared by Sumia Abdalsalam Alfitoury / Libya
Viruses that infect and parsitized bacteria is known as bacteriophage.
It was discovered by Frederick.W.Twort in Great Britian (1915) and Felix d’ Herelle in France(1917).
D’ Herelle coined the term bacteriophage meaning ‘bacterial eater’ to describe the agent’s bacteriocidal activity. He observed lysis of a broth culture of a dysentry bacillus.
al-salam alykom ..
this lecture starts with basic definitions in genetic , also talk about DNA & RNA ( structures , types , similarities and differences ) .
it talks about bacterial DNA ( chromosome structure / plamids structure and functions / transponon types )
later , discusses about central dogma / gene expression starting from genetic code/codons , then DNA replication , trancription and finally translation
prepared by Sumia Abdalsalam Alfitoury / Libya
Viruses that infect and parsitized bacteria is known as bacteriophage.
It was discovered by Frederick.W.Twort in Great Britian (1915) and Felix d’ Herelle in France(1917).
D’ Herelle coined the term bacteriophage meaning ‘bacterial eater’ to describe the agent’s bacteriocidal activity. He observed lysis of a broth culture of a dysentry bacillus.
genetics is a study of heredity, by studying microbial genetics, which is the most basic, one can extrapolate it to complex genetic studies of complex biological systems. effect of mutagens on genes is eye opening
Cell notes for junior high school and beginning high school biology students. Introduction to cell biology, includes systems analysis, Cell theory, Characteristics of Living things as well as Compound Light Microscope use, and structure and function of cell organelles.
This presentation introduces young readers to several important text structures. Through examples, pictures, and practice, students will learn how to identify and use the text structures of sequence, description, compare and contrast, cause and effect, and problem and solution.
For a study guide and ready-to-use classroom texts, purchase my unit at http://www.teacherspayteachers.com/Product/Introduction-to-Text-Structure-451417
This power point presentation is an attempt to present some direct and some indirect evidences in favour of DNA as genetic material. Very few organisms have RNA as genetic material for example plant virus and some bacteriophages
Introduction to Genetic Material, Physical and Chemical properties of the same and various types of coiling mechanisms as well as information about chromosomal and extra-chromosomal DNA.
"Impact of front-end architecture on development cost", Viktor TurskyiFwdays
I have heard many times that architecture is not important for the front-end. Also, many times I have seen how developers implement features on the front-end just following the standard rules for a framework and think that this is enough to successfully launch the project, and then the project fails. How to prevent this and what approach to choose? I have launched dozens of complex projects and during the talk we will analyze which approaches have worked for me and which have not.
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Let's dive deeper into the world of ODC! Ricardo Alves (OutSystems) will join us to tell all about the new Data Fabric. After that, Sezen de Bruijn (OutSystems) will get into the details on how to best design a sturdy architecture within ODC.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
2. Introduction:
Bacteria have four important
advantages for "traditional types of
genetic experiments":
They are haploid (no masking).
New generation is produced every 20
minutes.
Easy to grow in ENORMOUS
NUMBERS.
Individual members of these large
populations are GENETICALLY
IDENTICAL (or very nearly so).
5. 3 MAJOR TYPES of genetic
transfer found in bacteria:
Transformation - this is what Avery
characterised remember? Somehow
or another, the bacteria can take up
externally provided DNA.
Conjugation - DNA is transferred
from one bacteria cell to another, via
"sex pilli".
Transduction - this is where DNA is
introduced into bacteria by injection
from a bacteriophage.
6. Bacterial Transformation
"Transformation" is simply the
process where bacteria manage to
"uptake" or bring in a piece of
external DNA (somehow or
another). Usually, this process is
used in the laboratory to introduce a
small piece of PLASMID DNA into a
bacterial cell.
7.
8. DNA is the genetic material -
The First demonstration of
bacterial transformation.
Experiments done by
Frederick Griffith (in London)
in 1928 found there were two
different types of the
bacterium Streptococcus
pneumoniae:
9. An "S" or SMOOTH coat strain,
which is lethal to the mice.
An "R" or rough strain, which will
not hurt the mouse.
11. However, if he were to take a mixture of the
heat-inactivated S strain,
mixed with the R strain, the bacteria would
die. Thus there was some
Material in the heat-killed S strain that was
responsible for "transforming" the R strain
into a lethal form.
12. Griffith (and a lab co-worker) was
killed in their laboratory in 1940
from a German bomb. However,
their work continued on in the U.S.,
and in 1944, Oswald Avery, C.M.
MacLeod, and M. McCarty carefully
demonstrated that the ONLY
material that was responsible for
the transformation was DNA - thus,
DNA was the "Genetic material" -
however, many scientists were still
not sure that it was REALLY DNA
(and not proteins) that was the
genetic material. responsible for
"transforming" the R strain into a
lethal form.
13. McCarty,M., The Transforming
Principle - Discovering that
Genes are made of DNA, (New
York: W.W.Norton & company,
1985) - Although this book was
written about 40 years after the
experiments took place (1985),
it is an excellent history of the
research that was going on in
the early 1940's
14. The genetic transfer of streptomycin resistance (strr) to the
streptomycin sensitive (strs) cells of E.coli.
The recovery of strs cells depends on the concentration of
the strr DNA.
15. Co-transformation is simply the
simultaneous transformation of two
different DNA fragments.
First, you must obtain your DNA - you
can do this by isolating DNA from a nice
bacteria that has some DNA you want to
use....
First, you must obtain
you DNA - you can do
this by isolating DNA
from a nice bacteria that
has some DNA you
want to use....
16. Now you do the transformation and
have a look at the products - if you're
transforming into a strain that lacks the
gene of interest (which you usually are),
then the process is quite easy - just look
for colonies that carries the trait of
interest:
17.
18. Bacterial Conjugation
Bacterial conjugation is the
pocess in which DNA is
transferred from a bacterial
donor cell to a recipient cell by
cell-to-cell contact. It has been
observed in many bacterial
species and is best understood
in E.coli, in which it was
discovered by Joshua
Lederberg in 1951.
19.
20. "SEX in a Blender"
By some clever use of timing
experiments, it is possible to generate
a genetic map of E.coli!