Mitochondria are double-membrane bound organelles found in the cells of eukaryotic organisms. They are known as the "powerhouse of the cell" as their main role is to produce energy in the form of ATP through oxidative phosphorylation. Key functions of mitochondria include hosting the Krebs cycle and electron transport chain to generate ATP, regulating cellular metabolism and apoptosis. Mitochondrial DNA is separate from nuclear DNA and is maternally inherited. Dysfunctions in mitochondrial genes and proteins can lead to various mitochondrial diseases.
Post-transcriptional modification or co-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an RNA primary transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule
DNA = Deoxyribonucleic acid (DNA) is a molecule that encodes the genetic instructions used in the development and functioning of all known living organisms
Post-transcriptional modification or co-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an RNA primary transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule
DNA = Deoxyribonucleic acid (DNA) is a molecule that encodes the genetic instructions used in the development and functioning of all known living organisms
Genetic code, Deciphering of genetic code, properties of genetic code, Initiation & termination of codons, Gene Mutation, non sense codon, release factors, Transition , Trans versions
DNA structure, the bonds involved and it seperationMohit Adhikary
DNA structure, and the bonds that stabilizes it. The structural components, units and the proteins involved. Types of DNA and its separation methods. Chargaffs rule and its application
In this section, we describe digestion and absorption of Nucleic Acids and Most of the slides are cited from:
1. Lippincott's Illustrated Review Biochemistry
2. U. Satyran Biochemistry
Dr. Haroon
Structure and functions of MitochondriaICHHA PURAK
This Power Point Presentation (PPT) entitled “Structure and Functions of Mitochondria” consists of 118 slides with following sub-heads
INTRODUCTION
HISTORY
ORIGIN AND EVOLUTION OF MITOCHONDRIA
SYNTHESIS OF MITOCHONDRIA
ISOLATION OF MITOCHNDRIA
SHAPE , SIZE AND NUMBER OF MITOCHONDRIA
STRUCTURE OF MITOCHONDRIA
CHEMICAL COMPOSITION OF MITOCHONDRIA
FUNCTIONS OF MITOCHONDRIA
MITOCHONDRIA –POWER HOUSE OF CELL
MITOCHONDRIAL DNA/ GENOME
TRANSPORT OF PROTEINS INTO MITOCHONDRIA
MITOCHONDRIAL INHERITANCE
MITOCHONDRIAL DISEASES IN HUMAN
SUMMARY
QUESTIONS
BOOKS CONSULTED
REFERENCES
Genetic code, Deciphering of genetic code, properties of genetic code, Initiation & termination of codons, Gene Mutation, non sense codon, release factors, Transition , Trans versions
DNA structure, the bonds involved and it seperationMohit Adhikary
DNA structure, and the bonds that stabilizes it. The structural components, units and the proteins involved. Types of DNA and its separation methods. Chargaffs rule and its application
In this section, we describe digestion and absorption of Nucleic Acids and Most of the slides are cited from:
1. Lippincott's Illustrated Review Biochemistry
2. U. Satyran Biochemistry
Dr. Haroon
Structure and functions of MitochondriaICHHA PURAK
This Power Point Presentation (PPT) entitled “Structure and Functions of Mitochondria” consists of 118 slides with following sub-heads
INTRODUCTION
HISTORY
ORIGIN AND EVOLUTION OF MITOCHONDRIA
SYNTHESIS OF MITOCHONDRIA
ISOLATION OF MITOCHNDRIA
SHAPE , SIZE AND NUMBER OF MITOCHONDRIA
STRUCTURE OF MITOCHONDRIA
CHEMICAL COMPOSITION OF MITOCHONDRIA
FUNCTIONS OF MITOCHONDRIA
MITOCHONDRIA –POWER HOUSE OF CELL
MITOCHONDRIAL DNA/ GENOME
TRANSPORT OF PROTEINS INTO MITOCHONDRIA
MITOCHONDRIAL INHERITANCE
MITOCHONDRIAL DISEASES IN HUMAN
SUMMARY
QUESTIONS
BOOKS CONSULTED
REFERENCES
MITOCHONDRIA ,STRUCTURE ,Mt DNA ,PROTEIN TRANSPORT,ETC,OXIDATIVE PHOSPHORYLATIONLIFE SCIENCES
introduction, structure , functions,how proteins are transported into mitochondria,functions,electron transport chain,oxidative phosphorylation with animated videos
Mitochondria , its importance in neurosciencesNitish kumar
mitochondrial role in neurological sciences, its structure, major functions and the neurological diseases caused by improper functioning. complications , manifestations and further control.
Although majority of DNA in most eukaryotes is found in the nucleus,some DNA is present within the mitochondria also. Here, I have included the basics and importance of the Organelle/ Mitochondrial DNA which can help you in better understanding of the same.
Exploring the Powerhouse of the Cell: Mitochondria Unveiled
This PowerPoint presentation is tailored for Bachelor of Science students, offering a comprehensive exploration of mitochondria, the cellular powerhouses. Covering fundamental concepts such as structure, function, and cellular respiration, the presentation delves into the pivotal role mitochondria play in energy production. Additionally, it discusses the evolutionary origins, dynamic nature, and the intricate interplay between mitochondria and other cellular components. With engaging visuals and concise explanations, this presentation aims to provide a solid foundation for students to comprehend the significance of mitochondria in cellular biology.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
1. Mitochondria ( Power house of cell)
Ahsan Bashir 19024160-005
Umair Asghar 19024160-009
Muhammad Zeeshan Ali Janjua 19024160-022
Muhammad Shoaib Kazim 19024160-038
3. Introduction
Double membrane bound organelle found in all
Eukayotic cell.
Derived from Greek word “Mito” mean “thread”
“chondrion” mean “ granule like”.
First Observed By Richard Altman (1894).
Term mitochondria was coined by Carl Benda (1898)
Mitochondria are know known to be more than Hub of Richard Altman
Energy metabolism.
Basically found in all body cells except Red blood cells.
They produce enzyme for the metabolic conversion of food
To energy.
Carl Benda
4. Origin Of Mitochondria
There are two hypothesis about the origin of mitochondria
1. Endosymbiotic hypothesis
2. Autogenesis hypothesis
Endosymbiotic hypothesis:
According to this theory, the large anaerobic prokaryotes ingested small
aerobic bacteria. This aerobic bacteria stabilize themselves with in and later
developed into mitochondria.
The mitochondria became the site of aerobic respiration.
7. Morphology of mitochondria
Feature Morphology
Size 0.05 – 1.0 µm in diameter.
Length 1 – 10 µm long
Number Depends on type, size and functional state of cell. E.g : an average
liver cell contain around 1500 mitochondria.
Maximum (Liver cell)
Minimum ( Ear cell)
Location They are present in all type of eukaryotic cell except red blood cells
9. Outer membrane:
Simple phospholipid bilayer
It encloses the mitochondria
Contain large number of integral protein structures called porins, which allows molecules
to freely diffuse from one side of the membrane to the other.
The mitochondrial outer membrane can associate with the (ER) membrane.
The ER-mitochondria calcium signaling and the transfer of lipids between the ER and
mitochondria.
Ions, nutrient molecules, ATP, ADP etc. can pass through the outer membrane with ease.
10. Intermembrane space
It is also known as Perimitochondrial space.
The space between inner membrane and outer membrane.
The space between inner and outer membrane is approximately 70 A.
Because the outer membrane is freely permeable to small molecules, the concentration of
small molecules such as ions and sugars in the intermembrane space is the same as cytosol.
11. Inner membrane
It is freely permeable only to oxygen, carbon dioxide and water.
The inner mitochondrial membrane contain protein that performs redox reactions in
oxidative phosphorylation, ATP synthase, transport protein and mitochondrion fusion and
fission protein.
Several antiport systems exist, allowing exchange of anions between the cytosol and the
mitochondrial matrix.
12. Cristae
The folding of the inner membrane that allows more surface area, enhancing its
ability to produce ATP.
The inner surface of cristae in the mitochondrial matrix contain small knob like
structure called F1 particle.
The electron transport chain and chemiosmosis are the process which help to
produce ATP in final step of cellular respiration, occur in the cristae of mitochondria.
13. Mitochondrial matrix
Contain enzymes for the synthesis of ATP, ribosomes, tRNAs and the
mitochondrial DNA.
Matrix components can diffuse to inner membrane complexes and transport
proteins within short time.
Kreb cycle and Oxidative phosphorylation occur in matrix.
16. Mitochondrial Genome:
Mitochondrial genome was discovered in 1960.
This genome consist of a circular chromosome, 16.5 kb in size that is located inside the
mitochondrial organelle, not in the nucleus.
Here we discuss:
Mitochondrial DNA
Mitochondrial genetic system
Biogenesis
Inheritance of mitochondria
17. Mitochondrial DNA:
Mitochondrial DNA are Small, Double stranded ,covalently closed ,
circular molecule.
The DNA in the cell nucleus does not code for the construction
of mitochondria.
Mitochondrial DNA makes up less than 1% of the total cellular DNA.
Most usually remains attached to inner mitochondrial membrane.
Mitochondrial DNA consist of two strands:
Heavy chain which contain Purines ( Adenine, Guanine)
Light chain which contain pyrimidine ( Cytosine, thyamine, uracil)
18. Mitochondrial genetic system
Mitochondria are unique in their own way containing their own circular DNA and their
own ribosomes.
It has 16569 bp, encode 37 gene and no intron.
Out of 37 genes, 13 genes encode protein, 22 for tRNA and 2 for ribosomal RNA
This system consist of molecular machinery needed to replicate and gene contain
in DNA.
This machinery includes the macromolecule needed for transcription and
translation.
19. Cont.…
Replication of mitochondrial DNA:
Mitochondria show d-loop replication.
Replication start at replication site of
H strand.
There are two model for DNA replication:
Displacement replication
Symmetric replication
20. Biogenesis:
Mitochondrial biogenesis is the process by which cell increase mitochondrial mass.
It was first described by john Holloszy in the 1960.
Mitochondrial biogenesis is activated by numerous different signals during the time of
cellular stress.
Similar to cells, mitochondria divide and fuse with other mitochondria maintaining their
number of cells.
Our understanding of mitochondrial fission and fusion has improved in recent years with
the development of in vitro assays for their study and the identification of proteins
required for both events.
The balance between fusion and fission is likely a major determinant of mitochondrial
number, length, and degree of interconnection.
21.
22. Inheritance Of mitochondria
Mitochondrial DNA is inherited maternally in most animals.
Fathers only give genes to their children but mothers give both genes and cytoplasm
through their egg cell.
Since mitochondria are in the cytoplasm and reproduce themselves they only are
inherited from mothers.
Hence this pattern of mtDNA inheritance is well known as “ maternal inheritance”.
23. Homoplasmy and Heteroplasmy:
One daughter cell may be chance receive mitochondria that contain only a pure population
of normal mtDNA or a pure population of mutant mtDNA is called homoplasmy.
The daughter cell may receive a mixture of mitochondria some with and some without
mutation is called heteroplasmy.
Homoplasmy Heteroplasmy
25. Function of mitochondria
Energy transducer of the cell- synthesis ofATP
Kreb Cycle
ETC system
Oxidative phosphorylation
Extra mitochondrial inheritance:
mtDNA contains plasma genes (extra chromosomalgenes)
Transmitted from mother to the offspring
26. SITE OF SEVERAL METABOLIC REACTIONS
Structure Functions
Outer membrane Oxidation of epinephrine
Degradation of tryptophan
Elongation of fatty acid
Inner membrane Oxidative phosphorylation
Matrix Kreb cycle
Detoxification of ammonia in urea cycle
27. Kreb Cycle
It is also called citric acid cycle or Tricarboxylic (TCA) cycle.
Occur in the matrix of mitochondria.
Before entering this cycle, Pyruvate (C3) loses 1 carbon atom → acetic
acid (C2)
Acetic acid + Coenzyme A (CoA) → Acetyl CoA
28. Cont.…
In this cycle, Oxaloacetate (C4) + acetyl group (C2) = citrate (C6)
The final product of this cycle is:
2 CO2
3NADH
1 FADH2
1 GTP
29. Oxidative Phosphorylation
Synthesis of ATP in the presence of oxygen is called oxidative phosphorylation.
Oxidative Phosphorylation occur in matrix of mitochondria
Basically, 3 events occur in this reaction
1) Oxidation of coenzymes
2) Movement of protons
3) ATP synthesis by ATP sythase
30. Cont…
1. Oxidation of Coenzyme:
The oxidation of co enzyme cause to move electrons
The final electron acceptor is molecular oxygen → is reduced to water
31. Cont..
2. Movement of protons:
Are pumped into the intermembrane space →electrochemical proton gradient (proton-
motive force)
Protons have to flow back into the matrix trough ATP synthase
32. Cont..
3. ATP synthesis by ATP sythase:
Protons flow trough the complex (chemiosmosis) which cause this kinetic energy rotates
the F1 subunit → synthesis of ATP from ADP + Pi .
The final product is ATP
33. ATP production from bioorganic molecules
1 g fatty acid:
9Kcal/37kJ
1 g carbohydrate:
4Kcal/17kJ
1 g protein:
4Kcal/17kJ
34. Additional functions
Mitochondria play a central role in many other metabolic tasks:
• Regulation of the membrane potential
• Apoptosis (programmed cell death)
• Signaling (regulation of gene expression)
• Regulation of cellular metabolism
• Steroid synthesis
35. Mitochondrial disease
Mutations in genes for mitochondrial proteins encoded by both mtDNAand nuclear DNA
causes disorders such as :
Stoke
Cardiomyopathy
Neuropathy
Hearing
Blindness
Myopathy ( Muscular weekness)