The first simple forms of life appeared on earth more then 3 billion years ago. Microscopic forms of life are present in vast numbers in nearly every environment like soil, water, food, air , etc.
Medical microbiology is the study of causative agents of infectious diseases of humans and their reactions to such infections. In other words it deals with etiology, pathogenesis, laboratory diagnosis, specific treatment and control of infection (immunization).
Biofilm formation has been implicated in persistent tissue infections such as chronic wound infection, chronic otitis media, chronic osteomyelitis, chronic rhinosinusitis, recurrent urinary tract infection, endocarditic and cystic fibrosis-associated lung infection.They are equally resistant to various antimicrobial treatments compared to their planktonic form
Microbiology
study of organisms too small to be seen by the naked eye.
Microbes or Microorganisms
commonly referred to as “germs” or “bugs”
include bacteria, viruses, fungi, algae, protozoa and helminths.
Prions (“infectious proteins”) are recent addition.
Springer Series on Biofilms: Vol. 9 - The Root Canal BiofilmLuis Chavez de Paz
The Root Canal Biofilm
Editors: Chávez de Paz, Luis E., Sedgley, Christine M., Kishen, Anil (Eds.)
Compiles all the basic information needed on root canal biofilms
Discusses the basic biology of root canal biofilms
One focus is on observational and experimental evidence of root canal microbial biofilms
Sheds some light on how infections caused by root canal biofilms are clinically treated and reviews the implementation of novel anti-biofilm approaches
The first simple forms of life appeared on earth more then 3 billion years ago. Microscopic forms of life are present in vast numbers in nearly every environment like soil, water, food, air , etc.
Medical microbiology is the study of causative agents of infectious diseases of humans and their reactions to such infections. In other words it deals with etiology, pathogenesis, laboratory diagnosis, specific treatment and control of infection (immunization).
Biofilm formation has been implicated in persistent tissue infections such as chronic wound infection, chronic otitis media, chronic osteomyelitis, chronic rhinosinusitis, recurrent urinary tract infection, endocarditic and cystic fibrosis-associated lung infection.They are equally resistant to various antimicrobial treatments compared to their planktonic form
Microbiology
study of organisms too small to be seen by the naked eye.
Microbes or Microorganisms
commonly referred to as “germs” or “bugs”
include bacteria, viruses, fungi, algae, protozoa and helminths.
Prions (“infectious proteins”) are recent addition.
Springer Series on Biofilms: Vol. 9 - The Root Canal BiofilmLuis Chavez de Paz
The Root Canal Biofilm
Editors: Chávez de Paz, Luis E., Sedgley, Christine M., Kishen, Anil (Eds.)
Compiles all the basic information needed on root canal biofilms
Discusses the basic biology of root canal biofilms
One focus is on observational and experimental evidence of root canal microbial biofilms
Sheds some light on how infections caused by root canal biofilms are clinically treated and reviews the implementation of novel anti-biofilm approaches
Microbiology is the study of all living organisms that are too small to be visible with the naked eye. This includes bacteria, archaea, viruses, fungi, prions, protozoa and algae, collectively known as 'microbes'.
Microbiology Medical Subject Department Development - www.biomed.fitBiomed Fit
Microorganism is the general term for all tiny microorganisms that are invisible or invisible to the naked eye. The structure is relatively simple, the individual is small (generally <0.1mm), and can be divided into prokaryotes, eukaryotes and non-cells according to their evolution level and traits
The process of human understanding of microorganisms
The hard-to-understand microbial world
Application of bacteria in engenetic engineering.pptx voice.pdfkhushbushastri
#Application of bacteria in genetic engineering
#Application of Genetic Engineering
#Genetic engineering of Bacteria
#Bacteria as biofactories: to produce insulin
for diabetic patients
#Genetically Engineered Bacteria Could Prevent Obesity
#Role of Restriction Enzymes
#Genetically-engineered bacteria :
can inhale CO2 from the air and convert it into energy.
#Genetically engineered E. coli
# Role of Genetically engineered bacteria help in the production of insulin
#producing large amounts of pure human proteins for use in medicine
#Most DNA manipulation takes place within bacterial plasmids before being transferred to another host
#Engineering bacteria : Use as a treatment of genetic diseases
#Animation of synthetic bacteria communicating
#Important of Genetic Engineering
#Important tool for natural scientists
#Application of Bacillus thuringiensis
Bacteriology studies of bacteria
# branch of microbiology
# study of bacteria
# application
# role of bacteriology
# bacteria
# Koch postulate
#History
# role of bacteriology
# branch of bacteriology
# Recombination bacteria
# pathogenetic bacteriology
Prokaryotic and Eukaryotic Cells
# Introduction
# Definition of Prokaryotic Cells
# Definition of Eukaryotic Cells
# Feature of Prokaryotic and Eukaryotic Cells
# Common Factor of Prokaryotic and Eukaryotic Cells
# Difference Prokaryotic and Eukaryotic Cells
# Cell structure of Prokaryotic & Eukaryotic Cells
# EXAMPLES OF Prokaryotic & Eukaryotic Cells
Monoclonal antibody
# Definition
# A general representation of the method used to produce monoclonal antibodies
#Applications
# Monoclonal antibodies for cancer.
#MAbs approved by the FDA for cancer include:
# Factor affecting monoclonal antibody immunogenicity
#Monoclonal Antibodies : 4 Types
# Monoclonal Antibodies : 4 Types images
Economic importance of bacteria
#Economic importance of bacteria
#Bacteria : economically important as these microorganisms are used by humans for many purposes.
#Beneficial uses of bacteria
#Genetic engineering :
#Biotechnology :
#Food processing :
#Bioremediation
#Industry importance of bacteria
#Fiber industry:
#Medicine (probiotics)
#Agriculture importance
Mitosis and meiosis
# 2 types of cell division
# definition of meiosis
# phases of meiosis
# definition of mitosis
# stages of mitosis
# different between mitosis and meiosis
# similarities between mitosis and meiosis
Actinomyces: Genus of the actinobacteria
# Prokaryotic organisms
#Actinomyces colonies : form fungus-like branched networks of hyphae
# known as a ray fungus
#Scientific classification
# life cycle of sporulating actinomycetes
#Characteristics and Functions of Actinomycetes
# Generally anaerobic bacteria
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
2. MEANING OF MICROBIOLOGY
Microbiology : from Greek word
μῑκρος, mīkros, :"small";
βίος, bios, :"life";
-λογία, -logia
Branch of biology
that dealing with
microscopic forms of life cells.
4. DEFINATION OF MICROBIOLOGY
• MICROBIOLOGY : study of microorganisms
• Unicellular : single cell
• Multicellular :cell colony
or
• Acellular : lacking cells.
6. TYPES OF MICROORGANISMS
EUKARYOTIC:
microorganisms possess membrane
bound organelles
Include :FUNGI & PROTISTS
PROKARYOTIC—
microorganisms: conventionally classified as
lacking membrane-bound organelles
Include : BACTERIA & ARCHAEA
VIRUS :variably classified as organisms,
considered as a either as very simple
microorganisms or very complex molecules.
7. APPLICATION OF MICROBIOLOGY
INDUSTRIAL FERMENTATION
Antibiotic production
Tissues Engineering
DRUG delivery
biotechnologically
important Enzymes such as
Taq Polumerase
Industrial production
of Amino Acids :eg
Alcohol, Vinegar and Dairy
Products Production
BIOPOLYMER Production
Microbial Biodegradation
Various strains of non-
pathogenic Clostridia can
infiltrate and replicate
within solid tumors.:
Clostridial vectors.
