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MICROBIOLOGY OF 
SEWAGE AND SEWAGE 
TREATMENT 
Pro. F. Tahir 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 1
Sewage or Wastewater Treatment 
• Introduction 
Sewage is waste matter carried in sewers or drains. It 
is a “mixture of water and solids separated from 
various types of water as a result of natural or 
artificial processes”. 
Sewage could be sludge or wastewater from: 
– Domestic used water and toilet wastes 
– Rainwater (surface runoffs) 
– Industrial effluent 
– Agricultural wastes 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 2
Purpose of treatment 
–Eliminate potential pathogens and toxins 
–Decrease nutrient content (reduce microbial growth) 
–Remove odour and chemical contaminants 
–Reduce B.O.D. 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 3
Nature and Characteristics of Sewage 
• The properties of sludge resulting from urban sewage 
wastewater treatment differ from place to place and 
depend on the structure of the population and its eating 
habits, season, diversity of industrial and agricultural units 
which overflow the wastewater into the urban sewage, on 
the way of its treatment in the purification plant, etc. 
• Sewage is 99.9% water and 0.02-0.04% solids and sewage 
sludge is formed at the wastewater sewage treatment 
plants by being evacuated through the sewage system. It 
contains mineral, organic, and biological impurities and 
microorganisms in soluble, insoluble and colloidal forms. 
12/2/2014 
MCB 503 - ENVIRONMENTAL 
MICROBIOLOGY 
4
Nature and Characteristics of Sewage continued 
• The important physical, chemical and biological 
characteristics of sewage are: 
Temperature 
The pH 
Colour and Odour 
Solids 
Nitrogen and Phosphorus 
Chlorides 
Toxic Metals and Compounds 
Organic Material 
• Biochemical Oxygen Demand (BOD) 
• Chemical Oxygen Demand (COD) 
12/2/2014 
MCB 503 - ENVIRONMENTAL 
MICROBIOLOGY 
5
Microorganisms found in sewage 
BACTERIA 
Salmonella typhimurium 
Vibrio cholerae 
Clostridium botulinum 
Escherichia coli 
Etc. 
NEMATODES 
Schistosomes, Guinea worm, 
Tapeworm, Ascaris etc. 
VIRUSES PROTOZOA 
Giardia lamblia 
Entomoeba histolytica 
Cryptosporidium parvum 
Etc. 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 6
B.O.D. Effects 
Biochemical oxygen demand (BOD): 
Amount of oxygen required for microbial 
decomposition of organic matter in sample 
• Oxygen is removed from water when organic matter is 
consumed by bacteria. 
• Low oxygen conditions may kill fish and other 
organisms. 
Raw sewage BOD: 300 to 400 mg/liter 
Natural water BOD: 5-10mg/L 
If you dump raw sewage into 
“receiving water”, the dissolved O2 
can be quickly depleted by microbes 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 7
Large Scale Wastewater Treatment 
Multi-series process 
• Primary (1°) treatment 
• Secondary (2°) treatment (4 methods) 
• Advanced treatment (3o treatment) 
Effluent (treated liquid) is 
discharged into water bodies 
Sludge (solid) is further 
treated in anaerobic digester 
and disposed of 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 8
Primary (1°) Treatment 
Filter & settle steps remove 
~50% of solids & 25% of BOD 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 9
Anaerobic Sludge Digestion 
• Anaerobic organisms act on solids (sludge) 
• Various populations act sequentially 
Organic matter  organic acids, CO2, H2 
Organic acids  acetate, CO2, H2 
Acetate, CO2, H2 
 methane 
• Remaining sludge dehydrated 
• Disposal: incineration, landfill, fertilizer 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 10
Secondary (2°) Treatment 
• Eliminates most of remaining BOD 
• Microbial degradation of organic 
material 
• 4 different options 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 11
2° Treatment cont. 
12/2/2014 MCB 503 - ENVIRONMENTAL 
MICROBIOLOGY 
12
Tertiary or Advanced Treatment 
Physical, chemical or biological processes 
Increased expense over 1° and 2 ° treatment 
Removal of ammonia, 
nitrates and phosphates 
• Ammonia stripping: Liberates 
gaseous ammonia from water 
• Denitrification: use of bacteria 
(creation of N2 gas) 
• Chemical precipitation: 
phosphate removal 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 13
Disinfection 
Performed before effluent is discharged. 
• Chlorine 
• Ozone 
• Ultraviolet light 
Purpose: reduce numbers of 
microorganisms and viruses 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 14
THANK 
YOU 
FOR LISTENING 
12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 15

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Microbiology of sewage and sewage treatment

  • 1. MICROBIOLOGY OF SEWAGE AND SEWAGE TREATMENT Pro. F. Tahir 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 1
  • 2. Sewage or Wastewater Treatment • Introduction Sewage is waste matter carried in sewers or drains. It is a “mixture of water and solids separated from various types of water as a result of natural or artificial processes”. Sewage could be sludge or wastewater from: – Domestic used water and toilet wastes – Rainwater (surface runoffs) – Industrial effluent – Agricultural wastes 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 2
  • 3. Purpose of treatment –Eliminate potential pathogens and toxins –Decrease nutrient content (reduce microbial growth) –Remove odour and chemical contaminants –Reduce B.O.D. 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 3
  • 4. Nature and Characteristics of Sewage • The properties of sludge resulting from urban sewage wastewater treatment differ from place to place and depend on the structure of the population and its eating habits, season, diversity of industrial and agricultural units which overflow the wastewater into the urban sewage, on the way of its treatment in the purification plant, etc. • Sewage is 99.9% water and 0.02-0.04% solids and sewage sludge is formed at the wastewater sewage treatment plants by being evacuated through the sewage system. It contains mineral, organic, and biological impurities and microorganisms in soluble, insoluble and colloidal forms. 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 4
  • 5. Nature and Characteristics of Sewage continued • The important physical, chemical and biological characteristics of sewage are: Temperature The pH Colour and Odour Solids Nitrogen and Phosphorus Chlorides Toxic Metals and Compounds Organic Material • Biochemical Oxygen Demand (BOD) • Chemical Oxygen Demand (COD) 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 5
  • 6. Microorganisms found in sewage BACTERIA Salmonella typhimurium Vibrio cholerae Clostridium botulinum Escherichia coli Etc. NEMATODES Schistosomes, Guinea worm, Tapeworm, Ascaris etc. VIRUSES PROTOZOA Giardia lamblia Entomoeba histolytica Cryptosporidium parvum Etc. 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 6
  • 7. B.O.D. Effects Biochemical oxygen demand (BOD): Amount of oxygen required for microbial decomposition of organic matter in sample • Oxygen is removed from water when organic matter is consumed by bacteria. • Low oxygen conditions may kill fish and other organisms. Raw sewage BOD: 300 to 400 mg/liter Natural water BOD: 5-10mg/L If you dump raw sewage into “receiving water”, the dissolved O2 can be quickly depleted by microbes 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 7
  • 8. Large Scale Wastewater Treatment Multi-series process • Primary (1°) treatment • Secondary (2°) treatment (4 methods) • Advanced treatment (3o treatment) Effluent (treated liquid) is discharged into water bodies Sludge (solid) is further treated in anaerobic digester and disposed of 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 8
  • 9. Primary (1°) Treatment Filter & settle steps remove ~50% of solids & 25% of BOD 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 9
  • 10. Anaerobic Sludge Digestion • Anaerobic organisms act on solids (sludge) • Various populations act sequentially Organic matter  organic acids, CO2, H2 Organic acids  acetate, CO2, H2 Acetate, CO2, H2  methane • Remaining sludge dehydrated • Disposal: incineration, landfill, fertilizer 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 10
  • 11. Secondary (2°) Treatment • Eliminates most of remaining BOD • Microbial degradation of organic material • 4 different options 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 11
  • 12. 2° Treatment cont. 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 12
  • 13. Tertiary or Advanced Treatment Physical, chemical or biological processes Increased expense over 1° and 2 ° treatment Removal of ammonia, nitrates and phosphates • Ammonia stripping: Liberates gaseous ammonia from water • Denitrification: use of bacteria (creation of N2 gas) • Chemical precipitation: phosphate removal 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 13
  • 14. Disinfection Performed before effluent is discharged. • Chlorine • Ozone • Ultraviolet light Purpose: reduce numbers of microorganisms and viruses 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 14
  • 15. THANK YOU FOR LISTENING 12/2/2014 MCB 503 - ENVIRONMENTAL MICROBIOLOGY 15

Editor's Notes

  1. Higher BOD-large amounts of degradable materials present
  2. Bacteria: typhoid, cholera (photo), leigeonella, botulism Protozoa: giardia (photo), entomoeba, cryptosporidium (diarrhea, bad in HIV+) Worms: schistosome (photo), guinea worm, tapeworm, ascaris Viruses: rotavirus (photo), hepatitis A, polio
  3. Sludge is removed for sludge digestion Liquid sent for secondary treatment Sludge digestion?
  4. (Fermentation & TCA cycle Many treatment plants use generated methane to power equipment Organic acids: Lactic acid, Acetic acid, citric acid May have concentrated heavy metals
  5. Can be used in combo Look up ozone antimicrobial activity: )3 made by passing O2 across UV light, made on site and bubbled through water, highly reactive because unstable (like H2O2)