The document summarizes microbiology techniques for culturing microbes and microscopy. It describes the five steps for culturing as inoculation, isolation, incubation, inspection, and identification. For microscopy, it explains how light and electron microscopes work, different microscope types, sample preparation including various staining techniques, and compares light vs electron microscopes.
Electron microscopy by SIVASANGARI SHANMUGAM.
Electron microscopy is a technique for obtaining high-resolution images of biological and non-biological specimens.
5. Electron spectroscopy for surface analysis.
Applications of scanning electron microscope Applications of Transmission electron Microscope Brief history of electron microscopy Coherency and stability on the electron beam Different kinds of electron microscopes Different parts of electron microscope Effect of Brightness Electron Microscopy Electron Sources Field emission Interaction of electrons with Matter Limitations of Transmission electron Microscope Magnification contrast etc Material Characterization techniques Resolution Scanning electron microscope Scattering of electrons Specimen preparation of Transmission electron Microscope Thermionic Emission Various sources of electron beams and Detectors
International Journal of Computational Engineering Research (IJCER) is dedicated to protecting personal information and will make every reasonable effort to handle collected information appropriately. All information collected, as well as related requests, will be handled as carefully and efficiently as possible in accordance with IJCER standards for integrity and objectivity.
Electron microscopy by SIVASANGARI SHANMUGAM.
Electron microscopy is a technique for obtaining high-resolution images of biological and non-biological specimens.
5. Electron spectroscopy for surface analysis.
Applications of scanning electron microscope Applications of Transmission electron Microscope Brief history of electron microscopy Coherency and stability on the electron beam Different kinds of electron microscopes Different parts of electron microscope Effect of Brightness Electron Microscopy Electron Sources Field emission Interaction of electrons with Matter Limitations of Transmission electron Microscope Magnification contrast etc Material Characterization techniques Resolution Scanning electron microscope Scattering of electrons Specimen preparation of Transmission electron Microscope Thermionic Emission Various sources of electron beams and Detectors
International Journal of Computational Engineering Research (IJCER) is dedicated to protecting personal information and will make every reasonable effort to handle collected information appropriately. All information collected, as well as related requests, will be handled as carefully and efficiently as possible in accordance with IJCER standards for integrity and objectivity.
Study of magnetic and structural and optical properties of Zn doped Fe3O4 nan...Nanomedicine Journal (NMJ)
Objective(s):
This paper describes synthesizing of magnetic nanocomposite with co-precipitation
method.
Materials and Methods:
Magnetic ZnxFe3-xO4 nanoparticles with 0-14% zinc doping (x=0, 0.025, 0.05, 0.075, 0.1 and 0.125) were successfully synthesized by co-precipitation method. The prepared zinc-doped Fe3O4 nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM) and UV-Vis spectroscopy.
Results:
results obtained from X-ray diffraction pattern have revealed the formation of single phase nanoparticles with cubic inverse spinal structures which size varies from 11.13 to 12.81 nm. The prepared nanoparticles have also possessed superparamagnetic properties at room temperature and high level of saturation magnetization with the maximum level of 74.60 emu/g for x=0.075. Ms changing in pure magnetite nanoparticles after impurities addition were explained based on two factors of “particles size” and “exchange interactions”. Optical studies results revealed that band gaps in all Zn-doped NPs are higher than pure Fe3O4. As doping percent increases, band gap value decreases from 1.26 eV to 0.43 eV.
Conclusion:
These magnetic nanocomposite structures since having superparamagnetic property
offer a high potential for biosensing and biomedical application.
Study of magnetic and structural and optical properties of Zn doped Fe3O4 nan...Nanomedicine Journal (NMJ)
Objective(s):
This paper describes synthesizing of magnetic nanocomposite with co-precipitation
method.
Materials and Methods:
Magnetic ZnxFe3-xO4 nanoparticles with 0-14% zinc doping (x=0, 0.025, 0.05, 0.075, 0.1 and 0.125) were successfully synthesized by co-precipitation method. The prepared zinc-doped Fe3O4 nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM) and UV-Vis spectroscopy.
Results:
results obtained from X-ray diffraction pattern have revealed the formation of single phase nanoparticles with cubic inverse spinal structures which size varies from 11.13 to 12.81 nm. The prepared nanoparticles have also possessed superparamagnetic properties at room temperature and high level of saturation magnetization with the maximum level of 74.60 emu/g for x=0.075. Ms changing in pure magnetite nanoparticles after impurities addition were explained based on two factors of “particles size” and “exchange interactions”. Optical studies results revealed that band gaps in all Zn-doped NPs are higher than pure Fe3O4. As doping percent increases, band gap value decreases from 1.26 eV to 0.43 eV.
Conclusion:
These magnetic nanocomposite structures since having superparamagnetic property
offer a high potential for biosensing and biomedical application.
its about the microscopes types and there significance in the world for diagnostic purposes .advantages and disadvantages of the types of different microscopes
Introduction to microscopy
Different parts of a microscope & their function
Different types of microscopy
Different types of optical microscopy
Different types of electron microscopy
Different terms used in microscopy
Staining- Simple, Differential, Special
Gram Staining
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
1. Microbiology – Chapter 3
Culturing Microbes
The Five “I’s
Innoculation: Producing a pure culture
Isolation: Colony on media, one kind of microbe,
pure culture
Incubation: growing microbes under proper
conditions
Inspection: Observation of characteristics (data)
Identification: use of data, correaltion, to ID
organism to exact species
2. Microbiology – Chapter 3
Culturing Microbes
The Five “I’s
Innoculation: Producing a pure culture
Introduce bacteria into a growth medium using “aseptic technique” to
prevent contamination. Tools: Bunsen burner, loop. Needle, etc.
3. Microbiology – Chapter 3
Innoculation: Producing a pure culture
Introduce bacteria into a growth medium using “aseptic technique” to
prevent contamination. Tools: Bunsen burner, loop. Needle, etc.
4. Microbiology – Chapter 3
Isolation: Colony on media, one kind of
microbe, pure culture: isolation on general
and special “differential media”
General growth media: NA, TSA
Differential: Mac, EMB, SS
These have dyes, salts, inhibiting
agents : see differences on
plates
6. Microbiology – Chapter 3
Isolation: Colony on media, one kind of
microbe, pure culture – Streak Plates
7. Microbiology – Chapter 3
Isolation: Colony on media, one kind of
microbe, pure culture. Many colonies? Use
a needle, pick one, and redo streak plate
8. Microbiology – Chapter 3
Differential: Mac, EMB, SS
These have dyes, salts, inhibiting
agents : see differences on plates
9. Microbiology – Chapter 3
• Blood agar : rich with nutrients, can see a
difference, thus differential; much more
later
10. Microbiology – Chapter 3
• Incubation: Allow organisms to grow under the optimal
conditions
• Temperature, with or without oxygen etc
11. Microbiology – Chapter 3
• Incubation: Allow organisms to grow under the optimal conditions
• Temperature, with or without oxygen etc
• Candle jar reduces oxygen
15. Microbiology – Chapter 3
• Identification: Correlating data from all observations to
ID organism to species
• Resources: flow charts, Bergey’s manual etc.
• Ex. Gram – bacilli, ferments lactose, green sheen on
EMB: E.coli
16. Microbiology – Chapter 3
• Identification: Correlating data from all observations to
ID organism to species
• Gram + cocci, grape like clusters, golden yellow colonies, catalase
+, coagulase +, resistant to Methicillin (MRSA)
• Staphylococcus aureus
17. Microbiology Chapter 3, part 2
Microscopy
Light microscope: Visible Light is the energy source
18. Microbiology Chapter 3, part 2
Light can be described as a form of energy that moves in “waves” .
Wavelengths of light in the visible spectrum are used in most
microscopes. Remember the “prism”? Light is composed of different
colors of light. Each color has different wavelength. Longer
wavelengths have less energy (red end). Shorter; more energy (violet to
UV).
19. Microbiology Chapter 3, part 2
When light strikes an object the light can be:
Reflected – Bounces off (Mirror)
Transmitted – Passes through (GLASS)
Absorbed – Soaked (black colored paper)
Diffracted – Scattered as it passes through
(bugs on a dirty windshield)
Refracted – Bent as it passes (objects seen
under water) Glass lenses
Refractive index: degree of bending,
based on lens material and shape
of lens
20. Microbiology Chapter 3, part 2
So What? It is a big deal. When light in a scope strikes an
object (stained bacteria on a slide) some of the light is:
Absorbed A pattern is collected by the lenses and our
Refracted eyes see a magnified “object”
Diffracted
Reflected
Transmitted
21. Microbiology Chapter 3, part 2
Compound Light Microscope: Lens system with two
magnifying lenses, magnification is calculated by multiplying
the power of the two lenses (10 X 10 = 100 power)
22. Microbiology Chapter 3, part 2
Technicality
Contrast: Bacteria have little contrast unstained. Light is
only slightly refracted – diffracted – reflected etc. as it
passes through the cells. To see them we usually stain
them. Stains are colored dyes (chromophores) that increase
contrast. Without stains, special expensive microscopes
are needed.
Resolution: aka “resolving power” The ability of a lens
system to allow an observer to see fine detail. Quality of
lens systems (fine quality of glass and special lens
coatings). The best lens systems allow one to see two
points as distinct points eve when they are tiny and very
close together.
23. Microbiology Chapter 3, part 2
The best light microscopes can resolve objects to only about
0.2 – 0.5 microns. It is a function of the energy of visible
light and its wavelength (we make really good lenses). To
increase resolving power we need and energy source with
more energy (shorter wavelength) thus the electron
microscope.
24. Microbiology Chapter 3, part 2
The best magnification on our scopes is achieved with the
“oil immersion” objective. Oil is used with the lens because
it has “the same refractive index as glass”. We can see
objects with clarity at about 1000X magnification. Less light
is refracted away from the tiny lens and objects are “clearer”.
No oil = fuzzy poor quality image.
25. Microbiology Chapter 3, part 2
• Types of Light Microscopes
– Brightfield – most common, objects are dark
against a bright background
– Darkfield - special condenser, objects are
light against a dark background – used to see
live microbes unstained (spirochetes in fluid)
– Phase contrast – expensive condenser and
internal lens components, change “phase of
light”, so live specimens appear with more
internal contrast
– Fluorescence – fluorescent dyes and UV light
30. Microbiology Chapter 3, part 2
• Electron Microscope: energy source for magnification is a beam of
electrons (negative charged subatomic particles
31. Microbiology Chapter 3, part 2
• Transmission electron microscope – very
high magnification (100,000 X)
• Scanning: tremendous surface detail
• Transmission Scanning
32. Microbiology Chapter 3, part 2
• Tunneling scanning electron microscope
• Molecular and atomic level? Research
33. Microbiology Chapter 3, part 2
• Compare and contrast Light and Electron Microscope
• Light Electron
• Energy – light Energy – electron beam
• Cost - $1200 Cost – $120,000
• Simple to use Complex processes.
trained technician
• Magnification – 1200X Magnification – 100,000X
• Viewed by eye, camera Viewed with CRT, photos
35. Microbiology Chapter 3, part 2
• Preparation of samples for light microscope
• Wet mounts (ex. Hanging drop) for live observation
36. Microbiology Chapter 3, part 2
• Simple stain – one dye
• Differential stain – complex procedure, see
difference between cells
– Grams + and (-)
– Acid fast + and (-)
– Negative – acid dye stains background and
cells are white (cell wall repels stain)
– Capsule – modified negative stain to show
capsule layer