This document provides an overview of different types of microscopes used to observe microorganisms and describes various staining techniques. It discusses light microscopes like compound, darkfield, phase contrast, and fluorescence microscopes. Electron microscopes like transmission and scanning electron microscopes are also outlined. Different staining methods such as simple, differential, and special stains are summarized, with examples like Gram staining and acid-fast staining.
INTRODUCTION TO MICRO LAB, STAINING TECHNIQUES & MORPHOLOGY OF BACTERIADrBhavikapatel
This PPT is helpful to understand first practical to 2nd year MBBS student.
I have added 2 video in this PPT to understand staining techniques properly.
Reference: 1 Gram stain video: Dr.G Bhanu prakash animated medical videos
2. Zn stain video: sridhar Rao
STAINING TECHNIQUES IN MICROBIOLOGY & CELL BIOLOGYManu Bhardwaj
Although living microorganisms can be directly examined with the light microscope, they often must be fixed and stained to increase visibility of specific morphological features, and preserve them for future study.
Staining methods are helpful for presumptive identification of Microbes like Gram stain helps to identify Gram positive and Gram negative bacteria, similarly Z-N stain helps to identify acid fast bacilli and India ink preparation capsule observation of Cryptococcus neoformans.
INTRODUCTION TO MICRO LAB, STAINING TECHNIQUES & MORPHOLOGY OF BACTERIADrBhavikapatel
This PPT is helpful to understand first practical to 2nd year MBBS student.
I have added 2 video in this PPT to understand staining techniques properly.
Reference: 1 Gram stain video: Dr.G Bhanu prakash animated medical videos
2. Zn stain video: sridhar Rao
STAINING TECHNIQUES IN MICROBIOLOGY & CELL BIOLOGYManu Bhardwaj
Although living microorganisms can be directly examined with the light microscope, they often must be fixed and stained to increase visibility of specific morphological features, and preserve them for future study.
Staining methods are helpful for presumptive identification of Microbes like Gram stain helps to identify Gram positive and Gram negative bacteria, similarly Z-N stain helps to identify acid fast bacilli and India ink preparation capsule observation of Cryptococcus neoformans.
Microbiology lab report :
1- introduction (Gram Staining)
2-Requirements (Reagents)
3-Method
4-Observations
College of Medicine
Al-Imam Mohammad Bin Saud Islamic University
First year
2013 – 2014
by asem shadid
This is a rundown of some staining techniques used in microbiology, including simple staining, negative staining, gram staining, acid-fast staining, endospore staining, and flagellar staining.
this presentation involves a comprehensive outlines regarding the most common different methods used in diagnostic microbiology to stain bacteria and their structures
Dal catasto ai servizi analitici - Conferenza amfm 20150701Tomaso Bertoli
Per SINFI oltre al catasto vero è proprio è possibile identificare una serie di servizi ad alto valore aggiunto per tutti gli stakeholder.
Per vincere le resistenze a cedere informazioni strategiche e per giustificare lo sforzo necessario a condividere in maniera tempestiva i dati servono bastone e carota. Oltre al sistema basato su premi e penalità utilizzato da AEEG possiamo immaginare che i nodi del Sistema Informativo Federato delle Infrastrutture possano offrire agli operatori coinvolti dei servizi «analitici» a supporto dell’ operatività quotidiana.
Microbiology lab report :
1- introduction (Gram Staining)
2-Requirements (Reagents)
3-Method
4-Observations
College of Medicine
Al-Imam Mohammad Bin Saud Islamic University
First year
2013 – 2014
by asem shadid
This is a rundown of some staining techniques used in microbiology, including simple staining, negative staining, gram staining, acid-fast staining, endospore staining, and flagellar staining.
this presentation involves a comprehensive outlines regarding the most common different methods used in diagnostic microbiology to stain bacteria and their structures
Dal catasto ai servizi analitici - Conferenza amfm 20150701Tomaso Bertoli
Per SINFI oltre al catasto vero è proprio è possibile identificare una serie di servizi ad alto valore aggiunto per tutti gli stakeholder.
Per vincere le resistenze a cedere informazioni strategiche e per giustificare lo sforzo necessario a condividere in maniera tempestiva i dati servono bastone e carota. Oltre al sistema basato su premi e penalità utilizzato da AEEG possiamo immaginare che i nodi del Sistema Informativo Federato delle Infrastrutture possano offrire agli operatori coinvolti dei servizi «analitici» a supporto dell’ operatività quotidiana.
Ветрова А., ЛаринаА., Гонцова В. Проект для "КонсультантПлюс Илан"prasu1995
Проект для компании «КонсультантПлюс Илан» (Красноярск)
Авторы: Валерия Гонцова (ИнАрхДиз АлтГТУ), Алена Ветрова, Анастасия Ларина (Связи с общественностью АлтГУ)
Выполнен в рамках интенсивного практического курса «Мастерская рекламы-3», Барнаул, 2012
Журко А., Фоминых А. Проект для компании «КонсультантПлюс Илан»prasu1995
Проект для компании «КонсультантПлюс Илан» (Красноярск)
Авторы: Александр Журко (ИнАрхДиз АлтГТУ), Анастасия Фоминых (Связи с общественностью АлтГУ)
Выполнен в рамках интенсивного практического курса «Мастерская рекламы-3», Барнаул, 2012
chapter 2- Microscopy.pptx Microscopy related with medicineHarikantSingh4
It is about microscope in details about microscope. It includes simple microscope, fluoresence microscope, electron microscope and dark field microscope. These are described in details in this presentation. This presentation has images also.
Introduction to microscopy
Different parts of a microscope & their function
Different types of microscopy
Different types of optical microscopy
Different types of electron microscopy
Different terms used in microscopy
Staining- Simple, Differential, Special
Gram Staining
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Generating a custom Ruby SDK for your web service or Rails API using Smithyg2nightmarescribd
Have you ever wanted a Ruby client API to communicate with your web service? Smithy is a protocol-agnostic language for defining services and SDKs. Smithy Ruby is an implementation of Smithy that generates a Ruby SDK using a Smithy model. In this talk, we will explore Smithy and Smithy Ruby to learn how to generate custom feature-rich SDKs that can communicate with any web service, such as a Rails JSON API.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
26. Mordant - intensifies the stain or coats a structure to make it thicker and easier to see after it is stained Example: Flagella - can not normally be seen, but a mordant can be used to increase the diameter of the flagella before it is stained Salmonella typhosa Counterstain: a second stain applied to a smear, provides contrast to the primary stain.
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38. Any Questions?? If you have any Qs, please don’t hesitate to stop by my office
Editor's Notes
Now, over the years the microscope has evolved and scientists have developed different kinds of microscopes to adequately examine different organisms/specimens.
Dark field microscopy is a very simple yet effective technique and well suited for uses involving live and unstained biological samples, such as a smear from a tissue culture or individual water-borne single-celled organisms. Considering the simplicity of the setup, the quality of images obtained from this technique is impressive.
Phase contrast microscopy is an optical microscopy illumination technique in which small phase shifts in the light passing through a transparent specimen are converted into amplitude or contrast changes in the image. A phase contrast microscope does not require staining to view the slide. This type of microscope made it possible to study the cell cycle . Phase contrast images have a characteristic grey background with light and dark features found across the sample. Light and dark fringes appear around regions with a change in optical density, for example the boundary between water and a cell. This normally manifests as a light halo around a dark object.
is an optical microscopy illumination technique used to enhance the contrast in unstained, transparent samples . DIC works on the principle of interferometry to gain information about the optical path length of the sample, to see otherwise invisible features. A relatively complex lighting scheme produces an image with the object appearing black to white on a grey background. This image is similar to that obtained by phase contrast microscopy but without the bright diffraction halo.
A fluorescence microscope is an optical microscope used to study properties of organic or inorganic substances using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption
Direct fluorescent antibody ( DFA or dFA ) (also known as "Direct immunofluorescence" [1] ) is a laboratory test that uses antibodies tagged with fluorescent dye that can be used to detect the presence of microorganisms . This method offers straight-forward detection of antigens using fluorescently labeled antigen-specific antibodies. Because detection of the antigen in a substrate of patient sample (cellular smear, fluid or patient- inoculated culture medium) is the goal, DFA is seldom quantitative.
An electron microscope is a type of microscope that uses a particle beam of electrons to illuminate the specimen and produce a magnified image. Electron microscopes (EM) have a greater resolving power than a light-powered optical microscope , because electrons have wavelengths about 100,000 times shorter than visible light ( photons ), and can achieve better than 50 pm resolution [1] and magnifications of up to about 10,000,000x, whereas ordinary, non-confocal light microscopes are limited by diffraction to about 200 nm resolution and useful magnifications below 2000x.
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes
In staining for microscopic examination for diagnosis or research acid dyes are used to color basic tissue proteins in contrast to basic dyes, which are used to stain cell nuclei and some other acidic components of tissues
A simple stain consists of a solution of a single dye. Some of the most commonly used dyes are methylene blue, basic fuchsin, and crystal violet. Simple stains allow one to distinguish the shape (morphology) of the bacteria. For example, E. coli and Bacillus Subtillus are bacilli or rod-shaped bacteria. Many bacilli occur singularly, but chains may also be observed. Bacilli very greatly in length and diameter. Staphylococcus aureus and Streptococcus pneumoniae are cocci or spherical bacteria. Cocci may occur singularly, in pairs (as in Streptococcus pneumoniae) , or in clusters (as in Staphylococcus aureus) . R. rubrum is a spirillum or curved bacterium, Spirilla always occur singularly.
Differential stains are more complex than simple ones and use more than one stain to differentiate cellular components. They are used to examine structural differences between bacterial groups or to provide contrast to different structures within the same organism
As mentioned, Gram-negative bacteria generally possess a thin layer of peptidoglycan between two membranes (diderms). Most bacterial phyla are Gram-negative, namely the cyanobacteria , spirochaetes and green sulfur , most Proteobacteria (the exceptions in the Proteobateria are some members of the Rhickettsiales and the insect-endosymbionts of the Enterobacteriales ) and many other phyla. [5] [14] [ edit ] Gram-positive bacteria Main article: Gram-positive bacteria On the other hand, Gram-positive bacteria have generally a single membrane (monoderm) surrounded by a thick peptidoglycan The Gram stain procedure uses 3 different stains. These are crystal violet, Gram’s iodine, and safranin. The cells are first stained with crystal violet, then Gram’s iodine. Following a rinse in alcohol, to de-colorize the cells, the cells are then stained with safranin. The Gram stain procedure separates almost all bacteria into two large groups: the Gram-positive bacteria that stain blue and the Gram-negative bacteria that stain pink. Bacteria take up the Gram stain differently because they differ in cell wall composition. Gram-positive bacteria have a thick cell wall layer. Alcohol does not readily penetrate to decolorize the cell wall of the previously applied crystal violet stain. Gram-negative cells have a thinner cell wall through which the alcohol readily penetrates. The crystal violet is removed from these cell walls that are then stained with the safranin counterstain
It is helpful in diagnosing Mycobacterium tuberculosis since its lipid rich cell wall makes it resistant to Gram stain . It can also be used to stain few other bacteria like Nocardia . The reagents used are Ziehl–Neelsen carbolfuchsin , acid alcohol and methylene blue . Acid-fast bacilli will be bright red after staining
Negative staining is an established method, often used in diagnostic microscopy , for contrasting a thin specimen with an optically opaque fluid. For bright field microscopy , negative staining is typically performed using a black ink fluid such as nigrosin . The specimen, such as a wet bacterial culture spread on a glass slide, is mixed with the negative stain and allowed to dry. When viewed with the microscope the bacterial cells, and perhaps their spores , appear light against the dark surrounding background
