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TRANSMISSIONAND
SCANNING ELECTRON
MICROSCOPY: BASIC
PRINCIPLES.
By,
Padala Srinivas Gunaditya
4th B.Tech, Metallurgical Engineering ,JNTUH College of
Engineering , Hyderabad
Kukatpally, Hyderabad-500085,Telangana, India
eBehindTheTeachers’ Desk (eBTTD)
20th to 28th august , 2020
CSIR NML
Introduction
 Material Science is one of the most important research
areas Material Characterization techniques are one of
the most important aspects of material science
 Determination of material using various methods
including Optical microscopy, Metallurgical
microscopy, Fluorescent Microscopy, X-Ray Diffraction,
Transmission electron microscopy, Scanning electron
microscopy, atom probe Microscopy and most
advanced material characterization technique and
Nuclear spectroscopy.
 The Material Characterization techniques that we are
going to discuss in this article is Electron Microscopy.
BasicTerms in
Microscopy
:An
Introduction
Resolution is the minimum distance
between two points or objectives which
can be distinguished as two separate or
individual entities.
Magnification generally means Enlarging
the size of a given object to observe
features present in it.
Contrast: Contrast means the relative
change in light intensity between an
object and its background.
Electron
Microscopy :A
Brief History
 A brief history of electron microscopy
 Let's discuss briefly the important discovers that lead to electron
microscopy.
 In 1925: DE Broglie theorized that electrons had wave-like
characteristics.
 In 1927: Davisson & Germier; Thompson & Reid
 In 1932: Knoll and Ruska
 In 1936: First TEM, Metropolitan-Vickers EM 1 (UK)
 In 1939: Successful commercial models developed
 In the 1960s: attempts were made to construct High Voltage Electron
Microscopes (HVEM)
 In the 1980s: only 1 HVEM .
 In the 1990s: 3 HVMs
Different
kinds of
electron
microscopes
 High-resolution TEM
 High voltage electron microscope
 Intermediate voltage electron microscope
 . Scanning Transmission electron microscope
 Analytical electron microscope
Electron
microscopes
structure
An Illumination system
Electromagnetic coils
Specimen stage
 Magnification system
Vacuum pump
Image capturing Devices (Detectors).
Electron
Sources:
a)Thermioni
c Emission
Guns
b)Field
Emission
 Electron gun :Electron gun contains an electrode, and the electrode
contains three parts cathode anode and Wehnelt electrode.
 Field emission guns:
Electro
magnetic
lenses and
Interaction of
electrons with
Matter:
Scattering of
electrons
Effect of
Brightness,
Coherency and
Stability on
electron beam
Brightness: Brightness is the current
density per unit solid angle of source
Coherency: It is defining how well the
electron waves are in step.
Stability: We also need the stability of the
current coming from the source
Specimen
preparation
of
Transmissio
n electron
Microscope
 Ion Milling
 Electropolishing
 Ultra-microtome
Applications
of scanning
electron
microscope
and
Transmission
electron
Microscope
F-Doped Hydroxyapatite, chemical formula
Ca 10 (PO 4) 6 (OH) 2)

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Electron Microscopy